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Full-Text Articles in Animal Sciences
Comparing Calf Sex Ratio And Semen Sex Ratio Determined By Conventional Pcr, Tara Marie Taylor
Comparing Calf Sex Ratio And Semen Sex Ratio Determined By Conventional Pcr, Tara Marie Taylor
LSU Master's Theses
Thirty-nine ejaculates from 8 Holstein bulls were collected. Straws of semen (n=750) were distributed among dairies in three states. Ten straws per ejaculate were sent to Louisiana State University (LSU) Dairy Improvement Center for conventional polymerase chain reaction (PCR) analysis. Spermatozoal DNA was extracted and PCR analysis was done using one primer set amplifying a single copy 125 base pair (bp) section of the Bos taurus factor IX (Christmas factor) precursor (found on the X chromosome) and another primer set amplifying a single copy section of the Bos taurus sex determining region Y protein (SRY) gene (found on the Y …
Proportional Assessment Of X And Y Chromosome-Bearing Spermatozoa In Bull And Boar Ejaculates Using Conventional And Real-Time Pcr Techniques, Julius Braden Paul
Proportional Assessment Of X And Y Chromosome-Bearing Spermatozoa In Bull And Boar Ejaculates Using Conventional And Real-Time Pcr Techniques, Julius Braden Paul
LSU Doctoral Dissertations
Considerable variation in the percentage of Y-chromosome bearing spermatozoa (%Y-CBS) between ejaculates in the same male has been reported. Collection regime has been speculated to influence the degree of variation in %Y-CBS in bull ejaculates (Chandler et al., 1998). Experiments were designed to evaluate the effects of two collection regimes on %Y-CBS. Conventional PCR combined with gel electrophoresis and image analysis was employed to determine %Y-CBS in ejaculates from two bulls collected on 7-day intervals and two bulls on 21-day intervals. Real-time PCR technology was used to quantify %Y-CBS in the same ejaculates. Boar ejaculates were also analyzed with both …