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2014

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Full-Text Articles in Agriculture

Broadband In Nebraska: Current Landscape And Recommendations, Nebraska Information Technology Commission, Nebraska Broadband Initiative, Nebraska Public Service Commission, University Of Nebraska-Lincoln, Nitc Community Council, Nebraska Department Of Economic Development, Aim Dec 2014

Broadband In Nebraska: Current Landscape And Recommendations, Nebraska Information Technology Commission, Nebraska Broadband Initiative, Nebraska Public Service Commission, University Of Nebraska-Lincoln, Nitc Community Council, Nebraska Department Of Economic Development, Aim

Rural Futures Institute: Publications

N ebraska’s broadband vision is that residents, businesses, government entities, commu-nity partners, and visitors have access to affordable broadband service and have the necessary skills to effectively utilize broadband technologies.

Objectives

To increase economic development opportunities, create good-paying jobs, at-tract and retain population, overcome the barriers of distance, and enhance qual-ity of life in Nebraska by stimulating the continuing deployment of broadband technologies which meet the need for increasing connection speeds.

To increase digital literacy and the widespread adoption of broadband technolo-gies in business, agriculture, health care, education, government and by individu-al Nebraskans.

Goals

The following goals and targets help …


Health Professionals’ Roles In Animal Agriculture, Climate Change, And Human Health, Aysha Z. Akhtar, Michael Greger, Hope Ferdowsian, Erica Frank Dec 2014

Health Professionals’ Roles In Animal Agriculture, Climate Change, And Human Health, Aysha Z. Akhtar, Michael Greger, Hope Ferdowsian, Erica Frank

Michael Greger, MD, FACLM

What we eat is rapidly becoming an issue of global concern. With food shortages, the rise in chronic disease, and global warming, the impact of our dietary choices seems more relevant today than ever. Globally, a transition is taking place toward greater consumption of foods of animal origin, in lieu of plantbased diets. With this transition comes intensification of animal agriculture that in turn is associated with the emergence of zoonotic infectious diseases, environmental degradation, and the epidemics of chronic disease and obesity. Health professionals should be aware of these trends and consider them as they promote healthier and more …


Studies To Characterize Heavy Metal Content And Migration From Recycled Polyethyleneterephthalate, Michael John-Ross Whitt Dec 2014

Studies To Characterize Heavy Metal Content And Migration From Recycled Polyethyleneterephthalate, Michael John-Ross Whitt

Master's Theses

Packaging Materials account for 31% of the world’s municipal solid waste. Agencies like the Environmental Protection Agency (EPA) and the Agency for Toxic Substances and Disease Registry (ATSDR) are pushing for the increased use of recycled thermoplastic materials. Polyethylene terephthalate (PET) is a commonly recycled thermoplastic which is used to package ready-to-eat fruits and vegetables. Most recycled polyethylene terephthalate (RPET) packaging materials contain heavy metal catalysts, the most common being antimony. The recent increased use of recycled plastic materials has been suspected as the source of increased human heavy metal exposure. In this study, cadmium, chromium, nickel, lead and antimony …


Flax: Food And Fiber, Madeleine K. Charney Oct 2014

Flax: Food And Fiber, Madeleine K. Charney

Madeleine K. Charney

Highlights the versatile and nutritious flax plant, now cultivated worldwide.


Effects Of Extreme Climate Events On Tea (Camellia Sinensis) Functional Quality Validate Indigenous Farmer Knowledge And Sensory Preferences In Tropical China, Selena Ahmed, John Richard Stepp, Colin M. Orians, Timothy S. Griffin, Corene Matyas, Albert Robbat, Sean Cash, Dayuan Xue, Chunlin Long, Uchenna Unachukwu, Sarabeth Buckley, Edward J. Kennelly Oct 2014

Effects Of Extreme Climate Events On Tea (Camellia Sinensis) Functional Quality Validate Indigenous Farmer Knowledge And Sensory Preferences In Tropical China, Selena Ahmed, John Richard Stepp, Colin M. Orians, Timothy S. Griffin, Corene Matyas, Albert Robbat, Sean Cash, Dayuan Xue, Chunlin Long, Uchenna Unachukwu, Sarabeth Buckley, Edward J. Kennelly

Publications and Research

Climate change is impacting agro-ecosystems, crops, and farmer livelihoods in communities worldwide. While it is well understood that more frequent and intense climate events in many areas are resulting in a decline in crop yields, the impact on crop quality is less acknowledged, yet it is critical for food systems that benefit both farmers and consumers through high-quality products. This study examines tea (Camellia sinensis; Theaceae), the world’s most widely consumed beverage after water, as a study system to measure effects of seasonal precipitation variability on crop functional quality and associated farmer knowledge, preferences, and livelihoods. Sampling was conducted in …


Growing South Dakota (Fall 2014), College Of Agriculture &. Biological Sciences Oct 2014

Growing South Dakota (Fall 2014), College Of Agriculture &. Biological Sciences

Growing South Dakota (Publication of the College of Agriculture, Food and Environmental Sciences)

[Page] 2 Abundant Opportunities: Bright Ag & Bio Career Outlook Fuels Enhancements
[Page] 4 Future Focus: New Institute Supports Efforts In Science, Technology, Engineering, Math
[Page] 7 Grant Funding Updates
[Page] 8 Profiles in Leadership: Engaging Students Utilizing Problem-Based Learning
[Page] 9 Profiles in Leadership: The Value Of Experience-Based Learning
[Page] 10 Campus News
[Page] 12 Steps To Ensure Student Success: Support Fostered In Living-Learning Communities
[Page] 14 New Student Advising Model Gets High Marks
[Page] 15 Student To Student: Ag Bio Ambassadors Relish Role As Front Line Recruiters
[Page] 16 Remembering Dr. Robert Pengra: SDSU Alums Establish Tribute Scholarship …


African Starchy Foods, Gastric Emptying, And Starch Digestion In Malian Stunted Children, Fatimata Cisse Oct 2014

African Starchy Foods, Gastric Emptying, And Starch Digestion In Malian Stunted Children, Fatimata Cisse

Open Access Dissertations

Starch serves as the main energy source in cereal and tuber-rich diets, and its glycemic response profile has been associated with health-related conditions. Sorghum and millet are known to have relatively low starch digestibility, a potentially desirable property for controlling blood glucose response and providing sustained energy. Gastric emptying rates of traditional sorghum and millet-based African foods of the Sahelian region (couscous, thick and thin porridges made from millet and/or sorghum) were compared to those of non-traditional "modern" foods that are mostly consumed in urban areas using a non-invasive 13C-labelled octanoic acid breath test in healthy volunteers. The obtained results …


Prediction Of Parturition And Dystocia In Holstein-Friesian Cattle, And Cesarean Section In Dystocic Beef Cattle, Wen Han Mark Hiew Oct 2014

Prediction Of Parturition And Dystocia In Holstein-Friesian Cattle, And Cesarean Section In Dystocic Beef Cattle, Wen Han Mark Hiew

Open Access Dissertations

Dystocia is a major problem in the dairy industry as it causes livestock and economic loss. It is more frequently seen in primiparous cattle compared to their multiparous counterparts due to their smaller stature and the slow maturation of pelvic dimensions. In some instances, human intervention of the parturition process is imperative to avoid pain, injury, and mortality of the neonate and the dam. The ability to accurately predict dystocia and the time of parturition will ensure that timely assistance can be given to animals that are of high risk of dystocia.^ The present thesis contains four studies that explore …


Food Insecurity And Psychological Well-Being Among Women Living With Hiv/Aids On Antiretroviral Therapy In The Alabama Black Belt, Andrew A. Zekeri, Youssouf Diabate Sep 2014

Food Insecurity And Psychological Well-Being Among Women Living With Hiv/Aids On Antiretroviral Therapy In The Alabama Black Belt, Andrew A. Zekeri, Youssouf Diabate

Professional Agricultural Workers Journal

The objective of this research was to estimate the prevalence of food insecurity and determine if food insecurity is associated with psychological well-being among women living with HIV/AIDS. Survey data were collected from 268 women living with HIV/AIDS attending two clinics that provide medical and social support services to HIV-positive patients who live in 23 counties in Southeast Alabama. The results indicated that, using USDA food security scale, 54% of the women were food insecure. Multiple regression analysis results indicated that income, depressive symptoms, race, and participation in SNAP were significant predictors of food insecurity; employment and education were not …


Biofeedback Relaxation Techniques In Addressing Cortex-Hypothalamus Control Loop For Motivation Of Obesity Avoidance, Liljana Bozinovska, Ahmed Majekodunmi Sep 2014

Biofeedback Relaxation Techniques In Addressing Cortex-Hypothalamus Control Loop For Motivation Of Obesity Avoidance, Liljana Bozinovska, Ahmed Majekodunmi

Professional Agricultural Workers Journal

This research explores how the achievement motivation pathway in the cortex influences hypothalamic activity, which could influence appetite control and obesity avoidance. The objective was to examine the sympathetic/parasympathetic feedback loop, and the influence of various biofeedback relaxation techniques on lowering heart rate (HR) and electrodermal activity (EDA). The methodology used was a standard biofeedback mechanism with visual representation of the measured physiological parameters. The feedforward part of the said feedback loop is controlled by hypothalamus while the feedback part is evaluated by the cortex. The participants were young adults and the sample size was ten. The results revealed that …


Identification And Characterization Of Cysteine Protease Genes In Tobacco For Use In Recombinant Protein Production, Kishor Duwadi Aug 2014

Identification And Characterization Of Cysteine Protease Genes In Tobacco For Use In Recombinant Protein Production, Kishor Duwadi

Electronic Thesis and Dissertation Repository

Plants are an attractive host system for pharmaceutical protein production. Many therapeutic proteins have been produced and scaled up in plants at a low cost compared to the conventional microbial and animal based systems. The main technical challenge during this process is to produce sufficient level of proteins in plants. Low yield is generally caused by proteolytic degradation during expression and downstream processing of recombinant proteins. The yield of a human therapeutic protein interleukin (IL) -10 produced in transgenic tobacco leaves was found to be below the critical level, and is potentially due to degradation by tobacco cysteine proteases (CysPs). …


Exploring Germplasm Diversity To Understand The Domestication Process In Cicer Spp. Using Snp And Dart Markers, Manish Roorkiwal, Eric J. Von Wettberg, Hari D. Upadhyaya, Emily Warschefsky, Abhishek Rathore, Rajeev K. Varshney Jul 2014

Exploring Germplasm Diversity To Understand The Domestication Process In Cicer Spp. Using Snp And Dart Markers, Manish Roorkiwal, Eric J. Von Wettberg, Hari D. Upadhyaya, Emily Warschefsky, Abhishek Rathore, Rajeev K. Varshney

College of Agriculture and Life Sciences Faculty Publications

To estimate genetic diversity within and between 10 interfertile Cicer species (94 genotypes) from the primary, secondary and tertiary gene pool, we analysed 5,257 DArT markers and 651 KASPar SNP markers. Based on successful allele calling in the tertiary gene pool, 2,763 DArT and 624 SNP markers that are polymorphic between genotypes from the gene pools were analyzed further. STRUCTURE analyses were consistent with 3 cultivated populations, representing kabuli, desi and pea-shaped seed types, with substantial admixture among these groups, while two wild populations were observed using DArT markers. AMOVA was used to partition variance among hierarchical sets of landraces …


Volume 11, Number 1 (Spring/Summer 2014), Ut Institute Of Agriculture Jul 2014

Volume 11, Number 1 (Spring/Summer 2014), Ut Institute Of Agriculture

Tennessee Land, Life and Science Magazine

Issue Highlights:

  • The four pillars of the Institute of Agriculture
  • Alumna forges partnership amid Waldo Canyon fire
  • Fishery biologists revive a river


Growing South Dakota (Summer 2014), College Of Agriculture &. Biological Sciences Jul 2014

Growing South Dakota (Summer 2014), College Of Agriculture &. Biological Sciences

Growing South Dakota (Publication of the College of Agriculture, Food and Environmental Sciences)

[Page] 2 Extending Knowledge, Changing Lives: SDSU Extension Marks 100-Year Milestone
[Page] 5 Preparing for SDSU Extension’s Future
[Page] 5 SDSU College of Agriculture & Biological Sciences Administrative Team [Page] 6 Summer College News
[Page] 7 Profiles In Leadership: Joseph Cassady; Local Leadership Important For Ag’s Future
[Page] 8 Advancing Agriculture: SDSU Precision Ag Program Evolves, Expands [Page] 9 On The Front Line: ADRDL Leads Important Effort For Animal Health Diagnostics
[Page] 10 4-H Philanthropy: Supporters Continue Campaign For New 4-H Exhibit Hall [Page] 11 Growing Global Citizens: AgBio Courses Offer International Learning Opportunities
[Page] 12 Guidance For Gardeners: Several …


The 24th Annual Research Conference Abstract Booklet, Nikki Lynn Rogers Jun 2014

The 24th Annual Research Conference Abstract Booklet, Nikki Lynn Rogers

University of Gondar Research Conferences

Staff members, postgraduate and senior undergraduate students of the University, invited guests and speakers participated in the conference. The annual conference of the University is meant to share experiences in research activities among juniors and seniors, staff and students, and invited guests. It is also meant to motivate students and young faculty to engage in research and also to initiate and strengthen interdisciplinary collaborations. The findings of the studies and the resulting recommendations are expected to be used in solving the diverse societal problems we have been facing.

Research activities at the University of Gondar are primarily aimed at solving …


The Public Health Impacts Of Concentrated Animal Feeding Operations On Local Communities, Michael Greger, Gowri Koneswaran May 2014

The Public Health Impacts Of Concentrated Animal Feeding Operations On Local Communities, Michael Greger, Gowri Koneswaran

Michael Greger, MD, FACLM

Large-scale farm animal production facilities, also known as concentrated animal feeding operations (CAFOs), release a significant amount of contaminants into the air and water. Adverse health effects related to exposure to these contaminants among CAFO workers have been welldocumented; however, less is known about their impact on the health of residents in nearby communities. Epidemiological research in this area suggests that neighboring residents are at increased risk of developing neurobehavioral symptoms and respiratory illnesses, including asthma. Additional research is needed to better understand community-scale exposures and health outcomes related to the management practices and emissions of CAFOs.


The Human/Animal Interface: Emergence And Resurgence Of Zoonotic Infectious Diseases, Michael Greger May 2014

The Human/Animal Interface: Emergence And Resurgence Of Zoonotic Infectious Diseases, Michael Greger

Michael Greger, MD, FACLM

Emerging infectious diseases, most of which are considered zoonotic in origin, continue to exact a significant toll on society. The origins of major human infectious diseases are reviewed and the factors underlying disease emergence explored. Anthropogenic changes, largely in land use and agriculture, are implicated in the apparent increased frequency of emergence and reemergence of zoonoses in recent decades. Special emphasis is placed on the pathogen with likely the greatest zoonotic potential, influenzavirus A.


Evaluating Colostrum Quality With Niacin Supplementation In The Diet Of Dairy Cattle, Allison Marie Pike May 2014

Evaluating Colostrum Quality With Niacin Supplementation In The Diet Of Dairy Cattle, Allison Marie Pike

Student Research Projects

Dairy calves are born with a naive immune system, and must obtain passive immunity through ingestion of colostrum. Colostrum provides the newborn with antimicrobial proteins called immunoglobulins, such as immunoglobulin G (IgG), that protect the calf for the first few weeks of life. Calves that receive good quality colostrum (>50 g/L of IgG) are more likely to survive to adulthood. Niacin is a vasodilator that may increase blood flow to the mammary gland during production of colostrum in the mother. This experiment investigated the effect of niacin supplementation at a rate of 48 g/cow/day for 3 weeks before calving …


A Content And Visual Analysis Of Promotional Pieces Used To Promote The Arkansas Soybean Promotion Board, Amy Hughes May 2014

A Content And Visual Analysis Of Promotional Pieces Used To Promote The Arkansas Soybean Promotion Board, Amy Hughes

Graduate Theses and Dissertations

A communications campaign was developed for a commodity promotion board in the southern region of the United States with promotional pieces produced and disseminated by a third-party communications group to reach the general public, soybean producers, and animal agriculture producers target audiences through key themes and messages. A systematic, content-driven approach assessed the potential impact on perceptions of individuals. This study utilized a content and visual analysis based on semiotic theory to analyze creative pieces and focus groups to assess content quality and impact.

Many of the creative pieces displayed too many themes, and the themes did not accurately represent …


Genome-Wide Analysis Of The Variation In Host Genetics In Response To Experimental Challenges With Porcine Circovirus 2b, Taylor B. Engle May 2014

Genome-Wide Analysis Of The Variation In Host Genetics In Response To Experimental Challenges With Porcine Circovirus 2b, Taylor B. Engle

Department of Animal Science: Dissertations, Theses, and Student Research

Porcine circovirus is the primary causative agent responsible for inducing a group of associated diseases known as porcine circovirus associated diseases (PCVAD), which can have detrimental effects on production efficiency as well as lead to mortality. The variation in host genetics plays a role in the ability to initiate an effective immune response. The objective of this research was to identify major genetic variants and genes that influence immune response and PCVAD susceptibility. Commercial crossbred pigs (n=974) were experimentally infected with a PCV2b strain to analyze genetic sources of variation in PCVAD susceptibility. The strain used for experimental infection was …


Growing South Dakota (Spring 2014), College Of Agriculture &. Biological Sciences Apr 2014

Growing South Dakota (Spring 2014), College Of Agriculture &. Biological Sciences

Growing South Dakota (Publication of the College of Agriculture, Food and Environmental Sciences)

This issue contains the SDSU Extension 2013 Annual Report.

[Page] 2 The New SDSU Extension: Two Years Later, Change Has Brought New Successes
[Page] 4 Your 24-7 Connection: iGrow.org Makes SDSU Extension Easily Accessible, Responsive
[Page] 5 SDSU Regional Extension Centers: Your Front Door To SDSU
[Pages] 6-7 Leading The Challenge: Projects To Address Food Security, Financial Knowledge Underway
[Pages] 8-9 Regional Collaboration: SET Grants & Food Networks Initiated
[Pages] 10-11 Encouraging & Empowering: Native American Program Assists Reservations In Striving For Healthy Food, Healthy Communities & Youth Science Programming
[Page] 12 Evaluating Yields: Crop Performance Testing Provides Important, Unbiased …


Heterogeneity, Not Randomness, Sets Challenges For Quantitative Genetics And Epidemiology: A Response To Davey Smith’S “Gloomy Prospect”, Peter J. Taylor Mar 2014

Heterogeneity, Not Randomness, Sets Challenges For Quantitative Genetics And Epidemiology: A Response To Davey Smith’S “Gloomy Prospect”, Peter J. Taylor

Working Papers on Science in a Changing World

Social epidemiologist Davey Smith (2011) argues that epidemiologists should accept a gloomy prospect: considerable randomness at the individual level means that they should keep their focus on modifiable causes of disease at the population level. The difficulty epidemiology has had in moving from significant population-level risk factors to improved prediction of cases at an individual level is analogous to the lack of success in the search for systematic aspects of the non-shared environmental influences that human quantitative genetics claims overshadow common environmental influences (e.g., the family’s socioeconomic status which siblings have in common). This article responds to the argument and …


An Ethnobotanical, Ecological And Lc-Ms-Based Chemometric Investigation Of Phaleria Nisidai, A Traditional Adaptogen Containing Diterpene Esters From Palau, Micronesia, Daniel Kulakowski Feb 2014

An Ethnobotanical, Ecological And Lc-Ms-Based Chemometric Investigation Of Phaleria Nisidai, A Traditional Adaptogen Containing Diterpene Esters From Palau, Micronesia, Daniel Kulakowski

Dissertations, Theses, and Capstone Projects

An ethnobotanical, ecological and LC-MS-based chemometric investigation of Phaleria nisidai, a traditional adaptogen containing diterpene esters from Palau, Micronesia

Palau is a country with a rich heritage of traditional medicine still being practiced. One of the most popular and respected remedies in Palau is a tea made from fresh leaves of Phaleria nisidai Kaneh. (Thymelaeaceae). Interviews conducted to determine the use of this plant revealed that it is employed non-specifically to treat a variety of general health concerns. Its use as a prophylactic to keep away sickness, as a 'system cleaner', as well as for strength and energy indicate …


Phages Of Non-Diary Lactococci: Isolation And Characterization Of Phi L47, A Phage Infecting The Grass Isolate Lactococcus Lactis Ssp Cremoris Dpc6860, Daniel Cavanagh, Caitríona M. Guinane, Horst Neve, Aidan Coffey, R. Paul Ross, Gerald F. Fitzgerald, Olivia Mcauliffe Jan 2014

Phages Of Non-Diary Lactococci: Isolation And Characterization Of Phi L47, A Phage Infecting The Grass Isolate Lactococcus Lactis Ssp Cremoris Dpc6860, Daniel Cavanagh, Caitríona M. Guinane, Horst Neve, Aidan Coffey, R. Paul Ross, Gerald F. Fitzgerald, Olivia Mcauliffe

Department of Biological Sciences Publications

Lactococci isolated from non-dairy sources have been found to possess enhanced metabolic activity when compared to dairy strains. These capabilities may be harnessed through the use of these strains as starter or adjunct cultures to produce more diverse flavor profiles in cheese and other dairy products. To understand the interactions between these organisms and the phages that infect them, a number of phages were isolated against lactococcal strains of non-dairy origin. One such phage, ΦL47, was isolated from a sewage sample using the grass isolate L. lactis ssp. cremoris DPC6860 as a host. Visualization of phage virions by transmission electron …


Effects Of Sodium Hydroxide Pretreatment On Structural Components Of Biomass, Alicia A. Modenbach, Sue E. Nokes Jan 2014

Effects Of Sodium Hydroxide Pretreatment On Structural Components Of Biomass, Alicia A. Modenbach, Sue E. Nokes

Biosystems and Agricultural Engineering Faculty Publications

Pretreatment is a unit operation in the conversion of biomass to valuable products that utilizes various combinations of conditions, including chemicals, heat, pressure, and time, to reduce the recalcitrance of lignocellulose. Many such pretreatments have been developed over the years, as the operating conditions can be adapted so that lignocellulose is modified in ways unique to each pretreatment. By tailoring pretreatment conditions to achieve these modifications, the types of final products produced can be controlled. The purpose of this review is to provide a consolidated source of information for sodium hydroxide effects on lignocellulose. The structural characteristics of lignocellulose and …


The Journal Of Undergraduate Research: Volume 12 Jan 2014

The Journal Of Undergraduate Research: Volume 12

The Journal of Undergraduate Research

This is the complete issue of the South Dakota State University Journal of Undergraduate Research, Volume 12.


Shade Coffee: Update On A Disappearing Refuge For Biodiversity, Shalene Jha, Christopher M. Bacon, Stacy M. Philpott, V. Ernesto Méndez, Peter Läderach, Robert A. Rice Jan 2014

Shade Coffee: Update On A Disappearing Refuge For Biodiversity, Shalene Jha, Christopher M. Bacon, Stacy M. Philpott, V. Ernesto Méndez, Peter Läderach, Robert A. Rice

College of Agriculture and Life Sciences Faculty Publications

In the past three decades, coffee cultivation has gained widespread attention for its crucial role in supporting local and global biodiversity. In this synthetic Overview, we present newly gathered data that summarize how global patterns in coffee distribution and shade vegetation have changed and discuss implications for biodiversity, ecosystem services, and livelihoods. Although overall cultivated coffee area has decreased by 8% since 1990, coffee production and agricultural intensification have increased in many places and shifted globally, with production expanding in Asia while contracting in Africa. Ecosystem services such as pollination, pest control, climate regulation, and nutrient sequestration are generally greater …


Volume 10, Number 2 (Fall/Winter 2014), Ut Institute Of Agriculture Jan 2014

Volume 10, Number 2 (Fall/Winter 2014), Ut Institute Of Agriculture

Tennessee Land, Life and Science Magazine

Issue Highlights:

  • The four pillars of the Institute of Agriculture
  • Alumna forges partnership amid Waldo Canyon fire
  • Fishery biologists revive a river


Nebraska Bqa: Antibiotic Use Guidelines, Rob Eirich Jan 2014

Nebraska Bqa: Antibiotic Use Guidelines, Rob Eirich

Nebraska Beef Quality Assurance Program

Cattlemen have demonstrated a commitment to animal health through vaccination programs developed carefully in cooperation with their veterinarian. When animals must be treated with an antibiotic after disease diagnosis, producers need to follow important antibiotic use guidelines established through the National Beef Quality Assurance (BQA) program.


Nebraska Bqa: Effective Cattle Handling, Rob Eirich Jan 2014

Nebraska Bqa: Effective Cattle Handling, Rob Eirich

Nebraska Beef Quality Assurance Program

Cattlemen know the importance of proper cattle handling and its effect on animal health and performance. Beef Quality Assurance has been implementing effective cattle handling (low-stress) into educational programs since its establishment in the 1980s.