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Full-Text Articles in Agriculture
Identification Of Novel Seroreactive Antigens In Johne’S Disease Cattle By Using The Mycobacterium Tuberculosis Protein Array, John Bannantine, Joseph J. Campo, Lingling Li, Arlo Randall, Jozelyn Pablo, Craig A. Praul, Juan Antonio Raygoza Garay, Judith R. Stabel, Vivek Kapur
Identification Of Novel Seroreactive Antigens In Johne’S Disease Cattle By Using The Mycobacterium Tuberculosis Protein Array, John Bannantine, Joseph J. Campo, Lingling Li, Arlo Randall, Jozelyn Pablo, Craig A. Praul, Juan Antonio Raygoza Garay, Judith R. Stabel, Vivek Kapur
United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications
Johne’s disease, a chronic gastrointestinal inflammatory disease caused by Mycobacterium avium subspecies paratuberculosis, is endemic in dairy cattle and other ruminants worldwide and remains a challenge to diagnose using traditional serological methods. Given the close phylogenetic relationship between M. aviumsubsp. paratuberculosis and the human pathogen Mycobacterium tuberculosis, here, we applied a whole-proteome M. tuberculosis protein array to identify seroreactive and diagnostic M. avium subsp. paratuberculosis antigens. A genome-scale pairwise analysis of amino acid identity levels between orthologous proteins in M. avium subsp. paratuberculosis and M. tuberculosis showed an average of 62% identity, with more than half the orthologous proteins sharing …
Envelope Protein Complexes Of Mycobacterium Avium Subsp. Paratuberculosis And Their Antigenicity, Fernando L. Leite, Timothy A. Reinhardt, J. P. Bannantine, Judith R. Stabel
Envelope Protein Complexes Of Mycobacterium Avium Subsp. Paratuberculosis And Their Antigenicity, Fernando L. Leite, Timothy A. Reinhardt, J. P. Bannantine, Judith R. Stabel
United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications
Mycobacterium avium subsp. paratuberculosis (MAP) is the causative agent of Johne’s disease, a chronic enteric disease of ruminant animals. In the present study, blue native PAGE electrophoresis and 2D SDS-PAGE were used to separate MAP envelope protein complexes, followed by mass spectrometry (MS) to identify individual proteins within the complexes. Identity of individual proteins within complexes was further confirmed by MS upon excision of spots from 2D SDS-PAGE gels. Among the seven putative membrane complexes observed, major membrane protein (MAP2121c), a key MAP antigen involved in invasion of epithelial cells, was found to form a complex with cysteine desulfurase (MAP2120c). …
Monoclonal Antibodies Bind A Snp-Sensitive Epitope That Is Present Uniquely In Mycobacterium Avium Subspecies Paratuberculosis, John Bannantine, Judith R. Stabel, Elise A. Lamont, Robert E. Briggs, Srinand Sreevatsan
Monoclonal Antibodies Bind A Snp-Sensitive Epitope That Is Present Uniquely In Mycobacterium Avium Subspecies Paratuberculosis, John Bannantine, Judith R. Stabel, Elise A. Lamont, Robert E. Briggs, Srinand Sreevatsan
United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications
Due to a close genetic relatedness, there is no known antibody that detects Mycobacterium avium subspecies paratuberculosis (MAP), which causes Johne’s disease in cattle and sheep, and does not cross-react with other M. avium subspecies. In the present study, a monoclonal antibody (MAb; 17A12) was identified from mice immunized with a cell membrane fraction of MAP strain K-10. This antibody is 100% specific as it detected a 25-kDa protein in all 29 MAP whole cell lysates, but did not bind to any of the 29 non- paratuberculosis strains tested in immunoblot assays. However, the antibody revealed variable reactivity levels in …