Agriculture Commons^™

Towards The Quantification Of Estrone And 17Β-Estradiol Conjugates In Dairy Cattle Urine Sorptive Stir Bar Extraction And Gas Chromatography-Mass Spectometry, S.M. Istiak Ahmed

Masters Theses & Specialist Projects

The global concern regarding the presence of compounds with estrogenic properties in the environment has grown significantly. Endocrine-disrupting compounds (EDCs) are environmental pollutants that disrupt the endocrine systems of both wildlife and humans. Estrogens, with their potent estrogenic effects, are particularly noteworthy among the various substances with endocrine-disrupting properties. They are of concern due to their prevalence in the environment, especially due to dairy waste contamination. These lipophilic compounds can accumulate in livestock fat tissues, potentially entering the human food chain. Sulfated forms of estrone and β- estriol, which exhibit a higher degree of saturation than their non-conjugated counterparts, have …

Liquid Chromatography Mass Spectrometry Method Development For The Determination Of Β–Defensins In Bovine Milk, Elisha Ogbebor

Masters Theses & Specialist Projects

Bovine mastitis is caused by a wide range of pathogens which results in a substantial economic loss for the dairy cattle livestock industry. β-defensins are a part of the innate immune system and act as the first line of defense against mastitis in bovine. β-defensins (~6- 10kDa) are antimicrobial peptides that contain about 28 to 67 amino acids, the presence of six cysteine residues results in their three disulfide bonds and a β-sheet structure. A few β- defensins have been identified in cows based on the site of occurrence and time of expression in tissue, but not in milk. A …

Effects Of Exercise When Transitioning From Free Choice Tall Fescue (Lolium Arundinaceum) Grass Diet In Late Summer To Free Choice Tall Fescue Hay Diet In Late Fall On Plasma Fructosamine Concentrations, Insulin Levels, Body Weight, And Body Condition Scores Of Stock Horse Mares And Gelding, Riley Brown

Masters Theses & Specialist Projects

Equine exercise may influence a horse’s metabolic and physiological functions. Studies can help to prove this linkage, and to better understand the nutritional requirements of the horse. The parameters that are being evaluated are plasma-fructosamine concentrations, insulin levels, body weight (lbs.), and body condition score (BCS) as a measurement of obesity. Eight horses used in this study were split into two groups; Worked horses were exercised three times a week while the unworked horses were not exercised. All eight horses were fed an ad libitum diet of tall fescue grass and hay for 127 days.

Forage samples were collected to …

Liquid Chromatography With Tandem Mass Spectrometry Method Development For The Determination Of Β–Defensins In Bovine Milk, Symone T. Whalin

Masters Theses & Specialist Projects

Bovine mastitis, caused by a wide array of pathogens, results in a substantial economic loss for the dairy cattle livestock industry. β-defensins are a part of the bovine’s innate immune system and act as the first line of defense against mastitis. Only foundational research has been done on β-defensins’ ability to treat and prevent mastitis. There have been no analytical methods reported in the literature for analyzing β-defensins in bovine milk. This research aims to create an analytical approach to determine β-defensins in bovine milk. It is challenging to determine an analyte in a complex sample matrix, and milk is …

Evaluation Of Supplementing Whole Cottonseed Within Feedlot Rations During The Stockerphase Of Production With The Goal Of Increasing Average Daily Gain, Emily Lauren Cook

Masters Theses & Specialist Projects

Beef calves are weaned at 6-months of age and enter the stocker phase of beef production. Stocker producers have the primary goal of adding weight inexpensively to increase profit on weaned calves. Whole cottonseed is an inexpensive feed ingredient that is popularly top-dressed within dairy and finishing beef diets and is high in protein and energy. During three periods lasting 50-days (fall 2019, fall 2020, and spring 2020), freshly weaned calves were placed on a finishing ration with the goal of increasing average daily gain. Base rations were calculated at 25% tall fescue hay, 23% corn, and 52% distillers’ solubles. …

Bodyweight Management And Relationship To Health In The Horse (Equus Caballus), Beverly Ann Gartland

Masters Theses & Specialist Projects

The health of the horse is of interest to animal caretakers. Horses that fall within a prescribed range of bodyweight and body condition may be healthier than horses that are lower or higher. Two studies were conducted related to body weight and condition of the horse. The first study examined if restricting the amount of space available for horses to graze could reduce bodyweight without having a strong impact on time budgets of the horse or removing horses from pasture. This method was determined to be effective in preventing weight gain, although there was an increase in grazing behavior displayed …

Evaluating The Success Of Female Selected Sex-Sorted Semen At Western Kentucky University's Dairy Farm, Briley Loggan

Masters Theses & Specialist Projects

The purpose of this study was to evaluate the use of female selected sex-sorted semen and to determine the association of variables on the success of Western Kentucky University’s Dairy Farm. Official breeding and calving records (n=144) were used to determine the relation of lactation number, breeding season, breeding number, breeding year and semen type on pregnancy results, sex of offspring, and the mortality of the offspring. Previous research has shown pregnancy results can be influenced by lactation number, breeding season, number of breedings and semen type. Results from this study show that pregnancy results were not associated with lactation …

Quantification Of Tylosin Antibiotics In Cattle Waste, Appala Keerthi

Masters Theses & Specialist Projects

Antibiotics are used as prophylactic agents to promote growth and for treating infections in animals. However, the irrational use of antibiotics in livestock management is a significant cause of the development of antibioticresistant genes in the environment. Each year 2 million people suffer from the infections caused by bacteria which are resistant to antibiotics and 23,000 of these people are estimated to die because of antibiotic resistance. New drugs are continually coming into the market but are at the risk of developing resistance. Thus, there is a need for the development of analytical methods which can be used to monitor …

Effects Of Transitioning From A Free Choice Tall Fescue (Lolium Arundinaceum) Hay Diet In Late Winter To A Free Choice Spring Tall Fescue Pasture Diet On Plasma Fructosamine Concentrations, Body Weight, And Body Condition Scores Of Stock Horse Mares, Paige A. Smith

Masters Theses & Specialist Projects

Approximately half of all reported laminitis cases are the result of “grass founder” (laminitis associated with long-term over consumption of lush, early spring pastures). Elevated body weights (BW), body condition scores (BCS), and blood glucose concentrations have all been associated with the onset of grass founder. Plasma fructosamine concentrations (PFC) have recently been used as an indicator of long-term (14–21 d), mean blood glucose concentrations in horses and numerous authors have reported that elevated PFC were observed in laminitic horses (Murphy et al., 1997; Keen et al., 2004; Knowles et al., 2012). This study was conducted to evaluate the long-term …

Efficacy Of An Electronic Scarecrow On 4 Mammalian Crop-Raiders In Limpopo Province, South Africa, Merrie Renee Richardson

Masters Theses & Specialist Projects

In South Africa, 2 primate species, Chacma baboons (Papio ursinus) and vervet monkeys (Chlorocebus pygerythrus), and 2 nocturnal mammals, Cape porcupine (Hystrix africaeaustralis) and bushbuck (Tragelaphus scriptus), are among many species of crop raiders. Currently, cost-effective, non-lethal solutions are lacking. From June through December 2012, I installed novel electronic scarecrows on two commercial citrus orchards and a private reserve and used video-recording remote cameras to assess cropraiders’ reactions to them in Limpopo Province, South Africa. I used focal animal sampling data from treatment and control group animals to examine differences in activity …

Spatial Distribution Of Human Elephant Conflict (Hec) And Characterization Of Crop-Raiding Elephants In Kasigau Region, Kenya, Simon Kasaine Kagwa

Masters Theses & Specialist Projects

Human-elephant conflict (HEC) is complex and a serious elephant conservation concern across Africa and Asia where elephants are found. HEC occurs whenever people and elephants share common interests. For HEC to be ameliorated and elephant conservation to be successful locally and regionally, the distribution and implications of HEC should be understood. The purpose of this study was to determine the spatial distribution of HECs and characterize elephant herds in terms of herd size responsible for crop-raiding in Kasigau. The study was generally guided by two working a priori hypotheses: (1) farms near the bush edge or livestock watering points will …

The Effects Of Multispecies Grazing On Pasture Management And Utilization, Chelsea Williams

Masters Theses & Specialist Projects

Multispecies grazing research was conducted using meat-type goats (Capra hircus L.) and Jersey heifers (Bos taurus L.) to determine the relationships between multiple grazing treatments and pasture utilization. The study was conducted for 60 days on the Western Kentucky University Farm in Bowling Green, KY. Cattle and goats have shown to differ in grazing preferences and to be economically important to the area. Grazing treatments included goats and cattle grazing simultaneously, sequentially, and goats grazing alone. A typical established Kentucky pasture was utilized with no weed management practices employed. Predominant forage species included tall fescue (Festuca arundinacea Schreb.), orchardgrass (Dactylis …

Sensory Evaluations Of Usda Select Strip Loin Steaks Enhanced With Sodium And Potassium Phosphates And Usda Choice Strip Loin Steaks For Comparable Palatability Factors, Lindsey Reynolds

Masters Theses & Specialist Projects

The cyclic nature of the beef industry is dependent on the supply and demand transaction initiated by consumers’ acceptability of quality meat products. When purchasing beef at the grocery store, consumers are dependent on USDA grades and visual appraisal; consumers expect consistency in products. Upon this appraisal, quality is determined by the color, marbling content, and texture of a particular retail cut, as well as cooked characteristics of tenderness, flavor, and juiciness. Variability in meat tenderness is a major quality defect of beef (Morgan et al., 1991b; Smith et al., 1992). A common practice used by beef processors to extend …

A Comparison Of Pregnant Mare Serum Gonadotropin And P.G. 600® To Improve Reproductive Efficiency In Sows, Evan Tate

Masters Theses & Specialist Projects

Three hundred and nineteen sows were utilized to study the effects of pregnant mare serum and P.G 600 on reproductive efficiency. Sows were randomly allotted to treatments. Sows were evaluated for age (# of parities), breed composition and body condition score. Three sows who shared all three fields in common were randomly treated with one of three treatments the morning after weaning: 1. (S) Controls: 5ml of Sterile Saline Solution 0.9% 2. (M) Treatment (PMSG): 12ml of Pregnant Mare Serum 3. (P) Treatment (PG600)® 5ml of P.G. 600 (Intervet America Inc., Millsboro, DE) No differences were observed for total number …

Relationships Of Body Condition, Blood Glucose And Insulin Concentration Of Grazing Horses, Ashley Montfort

Masters Theses & Specialist Projects

A recent study has reported that blood glucose levels and founder in horses consuming forage/concentrate mixed diets are positively correlated (Pass et al., 1998). Other studies have reported body fat and insulin resistance are also positively correlated in horses and humans (Hoffman et al., 2003; Boshell et al., 1968; DeFonzo et al, 1991; Kahn et al., 2000). Few studies have monitored these relationships in horses consuming forage only diets, even though the incidence of grass founder is quite high in obese horses and ponies Four thin, four moderate, and four obese horses were grouped two mares and two geldings per …

Effects Of Training And Experience On Repeatable And Reliable Assessment Of Beef Cattle Body Condition Scores, Pete Burmeister

Masters Theses & Specialist Projects

In recent years, body condition scores (BCS) have been included in Expected Progeny differences (EPD) calculations for maintenance energy. This experiment was designed to identify the acuteness at which technicians of different skill levels can correctly quantify BCS in beef cattle. Thirteen students enrolled in agriculture at Western Kentucky University were assigned to three experimental technician groups based on training level. Five members of the colligate livestock judging team with extensive cattle backgrounds were BCS trained and assigned to the experienced level, four students were BCS trained by the WKU livestock judging coach and assigned to the trained level, and …

A Comparison Of Intrauterine And Cervical Artificial Insemination Rods On Farrowing Rate And Litter Size In Artificially Mated Sows, Robert Fitzgerald

Masters Theses & Specialist Projects

This experiment compared the effects of two artificial insemination (AI) rods on farrowing rate and number of piglets born per litter. Three hundred eighty-nine sows were allotted into two experimental groups based on parity, body condition, and breed of sire influence of the sows. One hundred ninety-three matings were performed using the experimental intrauterine rod, and one hundred ninety-six matings were performed using the traditional cervical rod as the control. Total number of piglets born per litter was measured after each sow farrowed, and the farrowing rates were calculated at the end of the study. Farrowing rates were 68% and …

The Effect Of Calf Morbidity On Feedlot Performance And Profitability, Jessica Gentry

Masters Theses & Specialist Projects

Two "Value-Added Calf (VAC) Programs were evaluated relative to feedlot performance and profitability. Two hundred seventy-three head of feeder calves were included in this study. Ninety-five Certified: Preconditioned for Health (CPH), ninety KCA Gold Tag and eighty-eight "Sale Barn" cattle were fed at Horton's Research Feedyard in Fort Lupton, Colorado. No background information regarding the health status of the Sale Barn cattle was known. Cattle were purchased in December 1997 and were entered in the Rocky Mountain Ranch-to-Rail program on January 6, 1998. The cattle were checked daily for illness and taken to a hospital pen for treatment if symptoms …

Synchronizing Estrus And Improving Subsequent Production And Performance With Prostaglandin F2 Alpha And Pregnant Mare Serum Gonadotropin, Bryan Kutz

Masters Theses & Specialist Projects

Two-hundred-seventy-two sows were utilized in two trials to evaluate the effects of prostaglandin and pregnant mare serum on reducing weaning to estrus interval, increasing the degree of estrus synchrony and improving fertility at estrus resulting in improved pregnancy rates and subsequent litter performance. Sows were randomly allotted to treatments. In trial 1, sows were assigned to one of three treatments: T1 (n=18; l0mg of PGF2α /hd 24-48 hrs. after parturition), T2 (n=20; lOmg of PGF 2 α /hd at weaning) and controls (n=20). No differences (P>.05) were observed for survival rate and 21D litter wt. among treated and the …

Repeatabilities Of Pelvic Area Measurements Between And Within Technicians With Varying Levels Of Experience, Steven Ftizner

Masters Theses & Specialist Projects

There has been some concern recently about the ability of technicians to accurately measure and rank prospective replacement heifers and herd bulls for pelvic area. Two studies were conducted to determine the repeatabilities between and within technicians with varying levels of experience in measuring pelvic areas.

In both studies, virgin yearling heifers were measured for pelvic height and width to determine pelvic area. In each study, technicians included a veterinarian with several years experience in reproductive physiology practice and Western Kentucky University students with varying levels of experience in rectal examinations. In the first study , the veterinarian used both …

Swine Breed Differences In Agglutination Titers Following Vaccination With Sheep Red Blood Cells And Pasteurella Multocida (Serotype A), Kenneth Stalder

Masters Theses & Specialist Projects

An investigation into the genetic differences in the humoral immune response of swine following vaccination with a sheep red blood cell solution (SRBC) and a commercially prepared Pasteurella multocida (serotype A) bacterin (PmA) was conducted on a total of 268 pigs from two individual trials. This study was also conducted to evaluate the humoral immune response of pigs to a non-pathogen (SPEC) and a known pathogen to swine (PmA). The pigs used in the first trial were from 22 litters born between January 1991 and July 1991. The pigs consisted of Hampshire x Yorkshire (n=114), purebred Yorkshire (n=70) and Hampshire …

Distribution Of Sexual Activities Within Estrus Of Dairy Cattle, Donna Durbin

Masters Theses & Specialist Projects

Previous studies of dairy cattle have indicated that heifers and cows in hot, cold and moderate weather had different patterns of sexual activities. The purpose of this study was to compare distribution of sexual activities within estrus of dairy cattle. Three groups of lactating cows in hot, cold and moderate weather and a group of heifers in cold weather were observed. Estrus for each individual was divided into 12 equal periods. Sexual activities for 3 and 6 hours before and after estrus were also observed. The numbers of mounts and distribution of total sexual activities was analyzed. Mounts received per …

The Relationship Between The Murine Primary Immune Response To Sheep Red Blood Cells & Heterosis, Russell Gailor

Masters Theses & Specialist Projects

Two inbred strains of mice, BALB/c and C57BL, were crossbred to produce Fl hybrids. This breeding scheme ensured a high degree of genetic heterozygosity as exemplified by the fact that the average weight of the Fl hybrids at 10 weeks of age was 10.1 percent greater that that of their contemporaries in the inbred parental strains.

The three lines of mice were then challenged with a 0.5 ml intraperitoneal injection of two-percent sheep red blood cells (SRBC). After a 15-day period the mice were bled and their sera were microtitrated for anti-SRBC agglutinin and the titers statistically analyzed. The Fl …

Studies On I) Dry Matter And Nitrogen Disappearance Of Six Soybean Protein Products In Situ And Ii) Contamination Of In Situ Dry Matter And Nitrogen Disappearance With Acid Detergent Fiber, James Coomer

Masters Theses & Specialist Projects

In experiment I), dry matter disappearance (DMD) and nitrogen disappearance (ND) of raw soybeans (RAW), solvent extracted soybean meal (SBM), heat treated whole soybeans (HT), mechanically extracted soybean meal (MEX), dry extruded soybeans (DEX), and wet extruded soybeans (TEX), were studied in situ for times of 3, 6, 12 and 24 h of rumen exposure. Five gram, air dry, samples were suspended in the rumen of a lactating Holstein cow fed a total mixed ration twice daily. The percent DMD for 24 h was as follows: RAW-85.9; SBM-56.6; HT-39.0; MEX-40.2; DEX-28.0; TEX-43.3. The greatest DMD was observed with RAW and …

Performance & Carcass Traits Of Swine Of Four Different Phenotypes & Three Usda Grades, Jon Althaus

Masters Theses & Specialist Projects

The purpose of this study was to compare the performance and carcass characteristics of swine of four different phenotypes and three USDA grades.

Hogs were placed into phenotypic groups of 24 each and penned by group. These pens were ideal, cutability, light muscled, fat, VS US and US The hops were fed a 16% corn and soybean meal ration fortified with appropriate minerals and vitamins. They were housed in a total confinement curtain-sided Kentucky plan building with a partially slatted floor. The hogs were weighed at 6-week intervals throughout the project to monitor average daily gain and pen feed efficiency. …

Use Of Soybean Meal, Raw Soybeans, & Heat-Treated Soybeans As Protein Supplements With & Without Niacin For Dairy Cows In Early Lactation, Daniel Aguilar

Masters Theses & Specialist Projects

Twenty three cows with an average of 2.7 lactations were used to measure the effects of different protein sources (soybean meal, soybeans, and heat-treated soybean) and supplementation with niacin on milk production, milk components (protein, fat, somatic cell counts), 4% fat corrected milk, solid corrected milk, dry matter intake, body weight changes, and rumen volatile fatty acids (acetate, propionate, butyrate).

Diets affected performance of cows. The results indicate, in general, that feeding heat-treated soybean and soybean meal to lactating dairy cows in early lactation resulted in greater milk production, milk protein, dry matter intake and less loss of body weight …

Comparisons Of Performance & Carcass Traits Of Littermate Boars & Barrows, Hiromi Hirano

Masters Theses & Specialist Projects

The purpose of this study was to determine differences between littermate boars and barrows for performance and carcass traits in the Iowa and Northeast Iowa Swine Testing Stations. Data were obtained from 917 litters including 1,804 boars and 917 barrows. The data included 1,086 boars and 581 barrows from the Iowa Station at Ames, Iowa, and 718 boars and 336 barrows from the Northeast Iowa Station at New Hampton, Iowa. The Iowa Station data were obtained over a period of 12 seasons from the fall of 1979 through the fall of 1985, and the data from the Northeast Iowa Station …

Analysis Of The Reproductive Efficiency Of The Dairy Herd At Western Kentucky University Farm, Gregorio Lagombra

Masters Theses & Specialist Projects

A study of the reproductive performance of 179 dairy cows during the period from December 1978 through March 1984 was conducted at the Western Kentucky University Farm, Bowling Green, Kentucky. The climatic data showed seasonal variation in temperature as follows: winter 3.10C, spring 15.7°C, summer 23.8°C, and fall 12.0°C. The seasonal humidity was similar for winter (81.2%), spring (83.4%), and fall (85.3%); but for summer it was considerably higher (89.4%).

The conception rate varied through all the months but was lowest during the summer months. Seasonal data for conception rate were obtained and the results were as follows: winter 54.1%, …

The Antibody Production By Swine In Response To Sheep Red Blood Cells, Deborah Seymour

Masters Theses & Specialist Projects

Two experiments were conducted to study the antibody response of pigs challenged with the general antigen sheep red blood cells (SRBC).

In one experiment SRBC's were injected at one of four sties: intramuscularly into the neck, intramuscularly into the ham, subcutaneously into the fore flank or rear flank. These treatments were repeated four weeks later. The antibody responses to the four treatments were determined by microtiter and analyzed statistically. No significant (P> 0. 10) differences were found among responses to the four treatments during the fourth and eighth weeks after injections, but a significant (P<0.05) difference was found during the fifth week among all four groups. Coefficients of correlation showed highly significant (P<.0.01) relationships between SRBC antibody titer during the fourth week after injections and increase in weight of test animals from the beginning of the study until slaughter.

In a second experiment pigs …

The Relationship Between Linear Measurements & Subjective Placings In Elite Angus Cattle, Ted Dyer

Masters Theses & Specialist Projects

The cattle shown from 1981 to 1984 at the American Angus Breeders' Futurity were studied to determine the relationship between linear measurements and the judge's subjective placings. Linear measurements taken prior to the showing included wither height, hip height, and body length for both bulls and heifers. In addition body weight and scrotal circumference were recorded for bulls. A total of 624 cattle were studied-- 407 heifers and 217 bulls.

The cattle were divided into five groups by age and sex to analyze the annual difference in means and standard deviations for all recorded measurements. Among all five groups of …