Agriculture Commons^™

Identification And Functional Analysis Of Thadh1 And Thadh4 Genes Involved In Tolerance To Waterlogging Stress In Taxodium Hybrid ‘Zhongshanshan 406’, Lei Xuan, Jianfeng Hua, Fan Zhang, Zhiquan Wang, Xiaoxiao Pei, Ying Yang, Yunlong Yin, David Creech

Faculty Publications

The Taxodium hybrid ‘Zhongshanshan 406’ (T. hybrid ‘Zhongshanshan 406’) [Taxodium mucronatum Tenore × Taxodium distichum (L.). Rich] has an outstanding advantage in flooding tolerance and thus has been widely used in wetland afforestation in China. Alcohol dehydrogenase genes (ADHs) played key roles in ethanol metabolism to maintain energy supply for plants in low-oxygen conditions. Two ADH genes were isolated and characterized—ThADH1 and ThADH4 (GenBank ID: AWL83216 and AWL83217—basing on the transcriptome data of T. hybrid ‘Zhongshanshan 406’ grown under waterlogging stress. Then the functions of these two genes were investigated through transient expression and overexpression. The results showed that the …

Light Emitting Diode (Led) Color And Broiler Growth: Effect Of Supplementing Blue/Green Led To White Led Light On Broiler Growth, Stress, And Welfare, Jill R. Nelson, Joey L. Bray, Juliette Delabbio, Gregory S. Archer

Faculty Publications

Light emitting diode (LED) lighting provides an affordable lighting option for use in commercial poultry production. However, more information is needed to understand the effects of LED color on broiler welfare and growth. Five consecutive flocks (1 in summer, 1 in fall, 2 in winter, and 1 in spring) of straight run Ross 708 × Ross 708 broilers were reared in commercial type barns for 45 D. For white only (WO) treatment, birds were reared under white LED only (Agrishift MLB). For white supplemented (WS) treatment, birds were reared under white LED (Agrishift MLB) in the center aisle, with supplemental …

The Effect Of Sodium Chloride On Hybrid Taxodium Selections, Anna Koonce, Edward Bush, David Creech

Faculty Publications

Saltwater intrusion is a major cause of coastal erosion. New hybrid bald cypress species have been shown to exhibit salinity tolerance compared to native bald cypress species. Planting these hybrids, or Nanjing Bot Garden genotype (NBG), trees where their roots can stabilize soil and preserve land may reduce coastal erosion in areas subjected to saltwater intrusion. The purpose of this experiment was to determine whether or not selected bald cypress NBG genotype tree species survive better than native bald cypress trees in areas inundated with saline water. Leaves were harvested, dried and analyzed for their elemental concentration. NBG genotype trees …

Effectiveness Of Plant Species For Removing Atmospheric Ammonia, Marife B. Anunciado, Sheryll B. Jerez, Hans Williams, Joey Bray, Dean W. Coble, Rena Saito

Faculty Publications

Six plant species of Yaupon, Eastern red cedar, American holly, Arizona cypress, Arborvitae and Roughleaf dogwood were utilized to determine their effectiveness in the removal of atmospheric ammonia. All species were exposed to three ammonia levels (1, 5 and 10 ppm) in an environmental chamber. Foliar ammonia content was quantified using an enzymatic technique. The effects of exposure to ammonia on the physiological responses (e.g. photosynthetic activity, stomatal conductance, and transpiration rate) of plants in ambient condition were also determined using an open design photosynthetic gas exchange system. Foliar ammonia content was significantly different among the six plant species (p<0.0001) with Eastern red cedar exhibiting the highest content. The physiological responses differed significantly depending on the plant species and the ammonia treatment level. The photosynthetic response of plants to the presence of ammonia was mixed. At low exposure level, all species except Arborvitae had decreased photosynthetic activity, reducing by as much as 44.5% for Yaupon. At the highest concentration, however, Yaupon’s photosynthetic activity improved by about 10%. Exposure to ammonia caused increased stomatal conductance and transpiration rate on American holly and Arizona cypress, making them more susceptible to water loss.

Associations Between Residual Feed Intake And Apparent Nutrient Digestibility, In Vitro Methane-Producing Activity, And Volatile Fatty Acid Concentrations In Growing Beef Cattle, Jocelyn R. Johnson, Gordon E. Carstens, Wimberly K. Krueger, Phillip A. Lancaster, Erin G. Brown, Luis O. Tedeschi, Robin C. Anderson, Kristen A. Johnson, Arieh Brosh

Faculty Publications

The objectives of this study were to examine the relationship between residual feed intake (RFI) and DM and nutrient digestibility, in vitro methane production, and volatile fatty acid (VFA) concentrations in growing beef cattle. Residual feed intake was measured in growing Santa Gertrudis steers (Study 1; n = 57; initial BW = 291.1 ± 33.8 kg) and Brangus heifers (Study 2; n = 468; initial BW = 271.4 ± 26.1 kg) fed a high-roughage-based diet (ME = 2.1 Mcal/kg DM) for 70 d in a Calan-gate feeding barn. Animals were ranked by RFI based on performance and feed intake measured …

Seed Yield Prediction Models Of Four Common Moist-Soil Plant Species In Texas, Daniel P. Collins, Warren C. Conway, Corey D. Mason, Jeffrey W. Gunnels

Faculty Publications

Seed production by moist-soil plant species often varies within and among managed wetlands and on larger landscapes. Quantifying seed production of moist-soil plants can be used to evaluate wetland management strategies and estimate wetland energetic carrying capacity, specifically for waterfowl. In the past, direct estimation techniques were used, but due to excessive personnel and time costs, other indirect methods have been developed. Because indirect seed yield models do not exist for moist-soil plant species in east-central or coastal Texas, we developed direct and indirect methods to model seed production on regional managed wetlands. In September 2004 and 2005, we collected …

Utilization Of Pyrosequencing To Monitor The Microbiome Dynamics Of Probiotic Treated Poultry (Gallus Gallus Domesticus) During Downstream Poultry Processing, Vamsy Priya Guttala, Enrique G. Medrano, Joey Bray, Beatrice Clack

Faculty Publications

Antibiotic growth promoters that have been historically employed to control pathogens and increase the rate of animal development for human consumption are currently banned in many countries. Probiotics have been proposed as an alternative to control pathogenic bacteria. Traditional culture methods typically used to monitor probiotic effects on pathogens possess significant limitations such as a lack in sensitivity to detect fastidious and non-culturable bacteria, and are both time consuming and costly. Here, we tested next generation pyrosequencing technology as a streamline and economical method to monitor the effects of a probiotic on microbial communities in juvenile poultry (Gallus gallus …

High Throughput Genomic Sequencing Of Bioaerosols In Broiler Chicken Production Facilities, Kate M. O’Brien, Michael S. Chimenti, Morgan Farnell, Tom Tabler, Thomas Bair, Joey Lynn Bray, Matthew W. Nonnenmann

Faculty Publications

Chronic inhalation exposure to agricultural dust promotes the development of chronic respiratory diseases among poultry workers. Poultry dust is composed of dander, chicken feed, litter bedding and microbes. However, the microbial composition and abundance has not been fully elucidated. Genomic DNA was extracted from settled dust and personal inhalable dust collected while performing litter sampling or mortality collection tasks. DNA libraries were sequenced using a paired-end sequencing-by-synthesis approach on an Illumina HiSeq 2500. Sequencing data showed that poultry dust is predominantly composed of bacteria (64–67%) with a small quantity of avian, human and feed DNA (< 2% of total reads). Staphylococcus sp. AL1, Salinicoccus carnicancri and Lactobacillus crispatus were the most abundant bacterial species in personal exposure samples of inhalable dust. Settled dust had a moderate relative abundance of these species as well as Staphylococcus lentus and Lactobacillus salivarius. There was a statistical difference between the microbial composition of aerosolized and settled dust. Unlike settled dust composition, aerosolized dust composition had little variance between samples. These data provide an extensive analysis of the microbial composition and relative abundance in personal inhalable poultry dust and settled poultry dust.

Exposure Of Workers To Dust And Bioaerosol On A Poultry Farm, Sheryll B. Jerez, Y. Cheng, Joey Bray

Faculty Publications

Poultry houses are known for generating excessive dust, which originates from bedding materials, fiberglass insulations, feed, dried fecal materials, and feather particles. Dust may contain microorganisms, including endotoxins, fungi, and bacteria, that may affect living things when inhaled. Dust that contains living organisms is referred to as bioaerosol, and its particle size may range from 0.5 to 100 µm. Respirable dust, which has an aerodynamic diameter of less than or equal to 4 µm, can travel to and be deposited in the gas-exchange region of the human respiratory system. This is of particular concern because of the greater health hazard …

Evaluation Of Electrostatic Particle Ionization And Biocurtain™ Technologies To Reduce Air Pollutants From Broiler Houses, Sheryll B. Jerez, W Faulkner, K. D. Casey, M S. Borhan, R. A. Smith

Faculty Publications

The continuing growth of poultry production, along with the increasing urbanization of rural areas, is leading to more odor-related complaints from neighboring communities and more scrutiny from policy makers. It is, therefore, in the best interest of poultry producers to look at control methods for abating odors. Previous studies have shown that substantial amounts of volatile and odorous compounds are adsorbed and transported by dust particles. Thus, by reducing the amount of dust emitted from poultry facilities such as broiler houses, odor may be reduced as well. The objective of this study was to evaluate the effectiveness of two commercially …

Measurement Of Particle Size Distribution In A Swine Building, Sheryll B. Jerez, Yuanhui Zhang, X Wang

Faculty Publications

The majority of the research in animal buildings has been on measured concentrations of contaminants that the workers and animals are exposed to; emission measurements have only gained attention in recent years due to potential federal regulations on air quality emissions from animal feeding operations (AFOs). The contribution of AFOs to ambient PM10 and PM2.5 entails reliable measurement of particle size distribution. The objective of this study was to measure and compare the size distribution of particulate matter (PM) at multiple locations inside and at the exhausts of a wean‐to‐finish commercial swine building. The particle size distribution was measured by …

Spatial And Temporal Distributions Of Dust And Ammonia Concentrations In A Swine Building, Sheryll B. Jerez, Yuanhui Zhang, X Wang

Faculty Publications

Pollutants, especially dust, are rarely uniformly distributed within ventilated air spaces due to non‐uniform flow fields, particle inertia, gravitational settling, and diffusion. Thus, selecting suitable sampling locations for representative sampling is a challenge. The objective of this study was to determine the spatial and temporal distributions of dust and ammonia concentrations(NH3) in a swine building. Results of this study are useful in the design of sampling strategies that require limited sampling locations and in studying pollutant transport. This study was conducted in a commercial swine building in Illinois. The total suspended particulate (TSP) matter and ammonia concentrations were measured at …

Performance Comparison Between The Use And Nonuse Of An Enteric Health Medication Program Across Five Consecutive Commercial Broiler Flocks, Joey Lynn Bray, C. S. Taylor, T. E. Cherry, J. Carey

Faculty Publications

A study was conducted to examine broiler performance and carcass yield across 5 consecutive commercial broiler flocks after the removal of roxarsone (ROX) and growth-promoting antibiotics (GPA) from the feed. Over a 1-yr period, approximately 552,000 broilers were reared in 4 solid-walled, tunnel-ventilated houses, divided into 2 paired-house facilities, and were assigned 1 of 2 dietary treatments. The treated group received basal diets containing salinomycin, ROX, and GPA, whereas the control group received the same diets without ROX and GPA. Average BW were recorded for 200 sample birds/treatment per flock at 18, 35, and 48 d of age. Average BW, …

Effects Of Intermittent Suckling On Sow And Piglet Performance, Erin G. Brown, Lindsey B. Krebs, Chris L. Boone, Ty Cauthen

Faculty Publications

The objective of this study was to evaluate effects of intermittent suckling on sow and litter performance. Seventeen crossbred sows and litters were randomly assigned to treatment groups seven days prior to weaning: continuous suckling (CS) and intermittent suckling (IS; litters removed for 6 hr each from day 21 to 28). Litters were weaned at 28 days of age. Feed and water were available to litters and sows at all times. Feed intake was recorded. Body condition scores were collected on sows before farrowing and at weaning. Number of days to return-to-estrus for the sows was also recorded. Litters were …

Influence Of A Modified Pot-In-Pot Production Strategy On Root Temperature And Growth Of Rhododendron × ‘Mrs. G.G. Gerbing’ In Full Sun, Brennan Whitehead, David Creech

Faculty Publications

No abstract provided.

Enzymes As Feed Additive To Aid In Responses Against Eimeria Species In Coccidia-Vaccinated Broilers Fed Corn-Soybean Meal Diets With Different Protein Levels, J. Parker, E. O. Oviedo Rondon, Beatrice A. Clack, S. Clemente-Hernandez, J. Osborne, J. C. Remus, H. Kettunen, H. Makivuokko, E. M. Pierson

Faculty Publications

This research aimed to evaluate the effects of adding a combination of exogenous enzymes to starter diets varying in protein content and fed to broilers vaccinated at day of hatch with live oocysts and then challenged with mixed Eimeria spp. Five hundred four 1-d-old male Cobb-500 chickens were distributed in 72 cages. The design consisted of 12 treatments. Three anticoccidial control programs [ionophore (IO), coccidian vaccine (COV), and coccidia-vaccine + enzymes (COV + EC)] were evaluated under 3 CP levels (19, 21, and 23%), and 3 unmedicated-uninfected (UU) negative controls were included for each one of the protein levels. All …

Intestinal Microbial Ecology Of Broilers Vaccinated And Challenged With Mixed Eimeria Species, And Supplemented With Essential Oil Blends, E. O. Oviedo Rondon, M. E. Hume, C. Hernandez, S. Clemente-Hernandez

Faculty Publications

Intestinal microbiota is an important component in the development of defense mechanisms in the gut mucosa. This project determined the dynamics of intestinal microbial communities (MC) of broilers vaccinated at first day of age with live oocysts of Eimeria species and fed diets supplemented with 2 specific essential oil (EO) blends, Crina Poultry (CP) and Crina Alternate (CA). Five treatments were analyzed: 1) unmedicated-uninfected (UU) control; 2) unmedicated-infected (UI) control; 3) vaccinated with Advent cocci-vaccine and without feed additive (COV) supplements; 4) vaccinated with Advent and supplemented with CP; and 5) vaccinated with Advent and supplemented with CA. The EO …

Effects Of Feed Additives And Mixed Eimeria Species Infection On Intestinal Microbial Ecology Of Broilers, M. E. Hume, S. Clemente-Hernandez, E. O. Oviedo Rondon

Faculty Publications

Evaluation of digestive microbial ecology is necessary to understand effects of growth-promoting feed. In the current study, the dynamics of intestinal microbial communities (MC) were examined in broilers fed diets supplemented with a combination of antibiotic (bacitracin methylene disalicylate) and ionophore (Coban 60), and diets containing 1 of 2 essential oil (EO) blends, Crina Poultry (CP) and Crina Alternate (CA). Five treatments were analyzed: 1) unmedicated uninfected control; 2) unmedicated infected control; 3) feed additives monensin (bacitracin methylene disalicylate) + monensin (Coban 60; AI); 4) EO blend CP; and 5) EO blend CA. Additives were mixed into a basal feed …

Responses Of Coccidia-Vaccinated Broilers To Essential Oil Blends Supplementation Up To Forty-Nine Days Of Age, E. O. Oviedo Rondon, S. Clemente-Hernandez, P. Williams, R. Losa

Faculty Publications

Coccidiosis control may become a greater problem as the use of growth-promoting antibiotics (GPA) and ionophores declines. Vaccination with live oocysts may turn into a popular alternative to the use of coccidiostats in broilers, although cocci vaccination is frequently linked to temporary lower performance in young flocks. This experiment evaluates the dietary supplementation of 2 specific essential oil (EO) blends (Crina Poultry and Crina Alternate), either as alternatives to GPA and ionophores (BMD + Coban) or as feed additives that help to improve the performance of cocci-vaccinated broilers. Live performance and lesion scores were observed. These 2 specific EO blends …

Requirements Of Sodium And Chloride By Leghorn Laying Hens, A. E. Murakami, M. I. Sakamoto, J. R. G. Franco, E. N. Martins, E. O. Oviedo Rondon

Faculty Publications

Sodium and Cl are low-cost nutrients with great influence on feed conversion ratio (FCR), eggshell quality, and excreta moisture. Actual values of dietary requirements of these minerals for commercial laying hens are not well defined. These requirements were reevaluated in a factorial experiment using corn-soybean meal basal diets. No significant influence of Na and Cl levels was observed on egg production (%), egg weight (g), or feed intake (g/d), but levels of these minerals had variable effects on FCR, eggshell quality, and excreta moisture. The optimum requirement of Na changed according to the variable evaluated and level of Cl used.

Effect Of Electrolyte Balance In Low-Protein Diets On Broiler Performance And Tibial Dyschondroplasia Incidence, A. E. Murakami, J. R. G. Franco, E. N. Martins, E. O. Oviedo Rondon, M. I. Sakamoto, M. S. Pereira

Faculty Publications

A proper dietary electrolyte balance (DEB) is essential to ensure an optimum acid-base equilibrium and broiler performance. In low-CP diets, this balance can be affected by reduction of soybean meal and inclusion of high levels of synthetic amino acids. Although, some studies have related low-protein diets supplemented with amino acids and DEB, these relations are not well explained, because some research demonstrates confusion about the deficiency and balance of nutrients. The objective of these experiments was to evaluate the DEB effects of diets with low levels of protein supplemented with amino acids on broiler performance and bone development. Results indicated …

Survey Of Pathogens In Poultry Litter In The United States, Mac Terzich, Melody J. Pope, Tim E. Cherry, Jessie Hollinger

Faculty Publications

Poultry litter is one of many components resulting from the production of broilers. Understanding poultry litter microbiological composition is very beneficial when attempting to improve the broiler's environmental conditions and searching for the best uses for this valuable industry by-product. The objective of this study was to collect samples of poultry litter throughout the United States and determine the presence of bacteria in the litter. Tests were conducted for total bacteria, Gram negative bacteria, Gram positive bacteria, Staphylococcus, Escherichia coli, and coliforms. Poultry litter samples were taken from 12 different regions throughout the United States and were analyzed …

An Evaluation Of The Presence Of Pathogens On Broilers Raised On Poultry Litter Treatment-Treated Litter, M. J. Pope, T. E. Cherry

Faculty Publications

Two trials were conducted to evaluate the presence of salmonella, campylobacter, and generic Escherichia coli on broilers raised on Poultry Litter Treatment (PLT®)-enhanced litter in comparison with those raised on untreated litter. Two Company A farms included three houses on each farm as the treated group and three houses per farm as controls. Two complete growouts were evaluated on each farm. The Company B study included 10 farms with two paired houses per farm, one house as the treated group and one house as the control. One growout was evaluated per farm. The pathogen sampling consisted of litter sampling and …

Eyespray Vaccination: Infectivity And Development Of Immunity To Eimeria Acervulina And Eimeria Tenella, H. David Chapman, Tim E. Cherry

Faculty Publications

The infectivity of a coccidiosis vaccine and its ability to immunize chickens against two species of Eimeria was examined. The vaccine was administered to newly-hatched chicks by spraying directly onto the eye. The method resulted in a high proportion of chicks infected with E. acervulina and E. tenella. Vaccinated birds reared in cages in the absence of reinfection did not develop immunity to either species by 4 wk of age, but birds reared in floor pens developed immunity to both E. acervulina and E. tenella.

Stand Risk Rating For The Southern Pine Beetle: Integrating Pest Management With Forest Management, Gordon L. Autry

Faculty Publications

Stand risk rating for the southern pine beetle.

Dendroctonus frontalis Zimm., is a first step toward dealing with a serious but sporadic insect problem. Two approaches, one utilizing readily available resource data, the other employing data obtained from aerial photographs, illustrate application of current knowledge to meet the protection needs of resource management-the primary role of integrated pest management. During a 36-month period in Louisiana, high-risk stands accounted for 13.4 infestations per 1,000 acres, four times the rate of low-risk stands; in Texas high-risk stands accounted for 9 .9 infestations per 1 ,000 acres during 1973-1978, almost five times the …