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Description Of Baetao-Manteiga 41 And ‘Yunguilla’ Superior Andean Common Beans For Tanzanian Production Environments, S. Nchimbi Msolla, Phillip N. Miklas, D. Fourie, M. Kilango, T. Porch

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

An international effort was initiated with the evaluation of a diverse set of largeseeded Andean common beans (Phaseolus vulgaris L.), the Andean Diversity Panel, in sub-Saharan Africa. Several entries in the panel have been selected for extensive characterization on the basis of high performance acrossmultiple location × year trials in Tanzania—conducted both on station and on farm. Baetao- Manteiga 41, tested asADP-190, has a commercialKablanketi (light purple speckled) seed type, and ‘Yunguilla’, tested as ADP-447, has a commercial Calima (red mottled) seed type. Both lines exhibited yield stability, vigorous growth under low fertility conditions, angular leaf spot resistance, andmoderate …

Suppression Of Aflatoxin Production In Aspergillus Species By Selected Peanut (Arachis Hypogaea) Stilbenoids, Victor S. Sobolev, R. S. Arias, Kerestin Goodman, Travis Walk, Valerie Orner, Paola C. Faustinelli, Alicia Massa

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Aspergillus flavus is a soil fungus that commonly invades peanut seeds and often produces carcinogenic aflatoxins. Under favorable conditions, the fungus-challenged peanut plant produces and accumulates resveratrol and its prenylated derivatives in response to such an invasion. These prenylated stilbenoids are considered peanut antifungal phytoalexins. However, the mechanism of peanut−fungus interaction has not been sufficiently studied. We used pure peanut stilbenoids arachidin-1, arachidin-3, and chiricanine A to study their effects on the viability of and metabolite production by several important toxigenic Aspergillus species. Significant reduction or virtually complete suppression of aflatoxin production was revealed in feeding experiments in A. flavus, …

The Lx Report Of The Bean Improvement Cooperative No. 60, March 2017.

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

CONTENTS

LX Annual Report of the Bean Improvement Cooperative xi

BIC Coordinating Committee Membership - 1957 to 2017 . xii

BIC Meritorious Service and Distinguished Achievement Award Recipients - 1957 to 2015 xiii

BIC Awards Nomination Request for 2017 xvi

BIC/NAPIA Meeting in 2017 . xix

In Memory of Guillermo E. Galvez-Enriquez xx

RESEARCH PAPERS FOR 2017

OPTIMIZING SPORULATION OF Pseudocercospora griseola IN VITRO • Paula F. de Pádua1, Rafael Pereira1, Luanna B. W. Gomes1 and Elaine A. de Souza1* 1

FINE MAPPING THE BROAD SPECTRUM ANTHRACNOSE RESISTANCE GENE IN AMENDOIM CAVALO 3 • Gilio, T.A.S.1, Oscar P. Hurtado-Gonzales2, Giseli …

Characterization Of Small Rna Populations In Non-Transgenic Andaflatoxin-Reducing-Transformed Peanut, Imana L. Power, Phat M. Dang, Victor S. Sobolev, Valerie Orner, Joseph L. Powell, Marshall C. Lamb, Renée S. Arias

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Aflatoxin contamination is a major constraint in food production worldwide. In peanut (Arachis hypogaea L.), these toxic and carcinogenic aflatoxins are mainly produced by Aspergillus flavus Link and A. parasiticus Speare. The use of RNA interference (RNAi) is a promising method to reduce or prevent the accumula-tion of aflatoxin in peanut seed. In this study, we performed high-throughput sequencing of small RNApopulations in a control line and in two transformed peanut lines that expressed an inverted repeattargeting five genes involved in the aflatoxin-biosynthesis pathway and that showed up to 100% less aflatoxin B1 than the controls. The objective was …

Chemical Interruption Of Late Season Flowering To Improve Harvested Peanut Maturity, Marshall C. Lamb, Ronald B. Sorensen, Christopher L. Butts, Phat M. Dang, C. Y. Chen, Renée S. Arias

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Peanut (Arachis hypogaea) is a botanically indeterminate plant where flowering, fruit initiation, and pod maturity occurs over an extended time period during the growing season. As a result, the maturity and size of individual peanut pods vary considerably at harvest. Immature kernels that meet commercial edible size specifications negatively affect quality during processing due to their increased propensity for off flavors, higher moisture and water activity, and variable roasting properties. As peanuts progress toward maturation, late season flowers set within 40 days till harvest will not have sufficient time to develop into mature, marketable pods prior to harvest. …

Century-Old Mystery Of Puccinia Striiformis Life History Solved With The Identification Of Berberis As An Alternate Host, Yue Jin, Les J. Szabo, Martin Carson

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

The life history of Puccinia striiformis remains a mystery because the alternate host has never been identified. Inoculation of grasses using aeciospores from naturally infected Berberis chinensis and B. koreana resulted in infection on Poa pratensis, producing uredinia typical of stripe rust caused by P. striiformis. Analyses using real-time polymerase chain reaction and DNA sequence confirmed the rust fungus as P. striiformis. Pycnia and aecia were produced on B. chinensis, B. holstii, B. koreana, and B. vulgaris after inoculation using germinating telia of P. striiformis f. sp. tritici. Wheat inoculated with aeciospores from B. chinensis resulted in …

Tillage Effects On Soil Quality Indicators And Nematode Abundance In Loessial Soil Under Long- Term No-Till Production, R. B. Mendoza, T. G. Franti, John W. Doran, Thomas O. Powers, C. W. Zanner

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Soil quality indicators and nematode abundance were characterized in a loessial soil under long-term conservation tillage to evaluate the effects of no-till, double-disk, chisel, and moldboard plow treatments. Indicators included soil electrical conductivity (EC), soil texture, soil organic matter (SOM), and total particulate organic matter (tPOM). Nematode abundance was positively correlated with EC, silt content, and total POM and negatively correlated with clay content. Clay content was the main source of variation among soil quality indicators and was negatively correlated with nematode abundance and most indicators. The gain in SOM in the no-till system amounted to 10887 kg over the …