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United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications
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Envelope Protein Complexes Of Mycobacterium Avium Subsp. Paratuberculosis And Their Antigenicity, Fernando L. Leite, Timothy A. Reinhardt, J. P. Bannantine, Judith R. Stabel
Envelope Protein Complexes Of Mycobacterium Avium Subsp. Paratuberculosis And Their Antigenicity, Fernando L. Leite, Timothy A. Reinhardt, J. P. Bannantine, Judith R. Stabel
United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications
Mycobacterium avium subsp. paratuberculosis (MAP) is the causative agent of Johne’s disease, a chronic enteric disease of ruminant animals. In the present study, blue native PAGE electrophoresis and 2D SDS-PAGE were used to separate MAP envelope protein complexes, followed by mass spectrometry (MS) to identify individual proteins within the complexes. Identity of individual proteins within complexes was further confirmed by MS upon excision of spots from 2D SDS-PAGE gels. Among the seven putative membrane complexes observed, major membrane protein (MAP2121c), a key MAP antigen involved in invasion of epithelial cells, was found to form a complex with cysteine desulfurase (MAP2120c). …