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Induction Of Phytoalexin Accumulation In Broad Bean (Vicia Faba L.) Cotyledons Following Treatments With Biotic And Abiotic Elicitors, Soner Soylu, Mark H. Bennett, John W. Mansfield
Induction Of Phytoalexin Accumulation In Broad Bean (Vicia Faba L.) Cotyledons Following Treatments With Biotic And Abiotic Elicitors, Soner Soylu, Mark H. Bennett, John W. Mansfield
Turkish Journal of Agriculture and Forestry
Broad bean (Vicia faba) cotyledons that were inoculated artificially with fungal pathogens or exposed to various abiotic agents were analysed for phytoalexin production. Biotic elicitors, such as Botrytis cinerea and B. allii, and abiotic elicitors, such as ultraviolet (UV) radiation (254 nm) and freezing-thawing, were used to induce phytoalexin accumulation. Wyerone and other wyerone derivatives were the major phytoalexins responding in broad bean cotyledons. The quantities of wyerone within elicitor-treated tissues were examined by thin layer chromatography. The highest amount of wyerone was induced by B. cinerea (943 \mug/g fresh weight). Treatment of cotyledons with UV radiation (452 \mug/g f.wt), …