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Determination Of Tobacco Alkaloid Enantiomers Using Reversed Phase Uplc/Ms/Ms, Huihua Ji, Ying Wu, Franklin Fannin, Lowell P. Bush
Determination Of Tobacco Alkaloid Enantiomers Using Reversed Phase Uplc/Ms/Ms, Huihua Ji, Ying Wu, Franklin Fannin, Lowell P. Bush
Kentucky Tobacco Research and Development Center Faculty Publications
Nʹ-Nitrosonornicotine (NNN), a carcinogenic tobacco-specific Nʹ-nitrosamine (TSNA), is on the FDA list of harmful and potentially harmful constituents (HPHCs). Nornicotine, a product of the demethylation of nicotine, is the immediate alkaloid precursor for NNN formation. Nicotine, nornicotine and NNN are optically active. The accumulation of the isomers of nicotine, nornicotine, and NNN impacts their biological activity. In this paper, we report the determination of tobacco alkaloid enantiomers (including nicotine, nornicotine, anabasine, and anatabine) in samples of different tobacco lines using a reversed phase ultra-performance liquid chromatography-tandem mass spectrometer (UPLC/MS/MS) method. Current method demonstates excellent detection capability for all alkaloid enantiomers, …