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Full-Text Articles in Agriculture
Determination Of Tobacco Alkaloid Enantiomers Using Reversed Phase Uplc/Ms/Ms, Huihua Ji, Ying Wu, Franklin Fannin, Lowell P. Bush
Determination Of Tobacco Alkaloid Enantiomers Using Reversed Phase Uplc/Ms/Ms, Huihua Ji, Ying Wu, Franklin Fannin, Lowell P. Bush
Kentucky Tobacco Research and Development Center Faculty Publications
Nʹ-Nitrosonornicotine (NNN), a carcinogenic tobacco-specific Nʹ-nitrosamine (TSNA), is on the FDA list of harmful and potentially harmful constituents (HPHCs). Nornicotine, a product of the demethylation of nicotine, is the immediate alkaloid precursor for NNN formation. Nicotine, nornicotine and NNN are optically active. The accumulation of the isomers of nicotine, nornicotine, and NNN impacts their biological activity. In this paper, we report the determination of tobacco alkaloid enantiomers (including nicotine, nornicotine, anabasine, and anatabine) in samples of different tobacco lines using a reversed phase ultra-performance liquid chromatography-tandem mass spectrometer (UPLC/MS/MS) method. Current method demonstates excellent detection capability for all alkaloid enantiomers, …
Agricultural Livelihoods In Harlan County: A Case Study Approach Of Two Farms, Heather Kinney
Agricultural Livelihoods In Harlan County: A Case Study Approach Of Two Farms, Heather Kinney
Theses and Dissertations--Community & Leadership Development
This thesis explores agricultural livelihoods in Harlan County with two case studies in order to challenge dominant narratives about Eastern Kentucky. Harlan County, and Appalachia more broadly, is often written about in terms of its relationship to extractive industries. Absentee landownership in Appalachia has been well documented, especially in the case of coal counties. However, the relationship between extraction and agricultural livelihoods in Appalachia warrants more attention. The story of agricultural livelihoods in the region is often pushed to the periphery much like the practices of “hillside” farming. While geography makes much of the land unsuitable for farming in Harlan …