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Field studies were conducted over a three-year period at a total of 82 site-years to evaluate the sulfur (S) status of representative Illinois soils. In addition, greenhouse studies were conducted using soils from two of the three years of field experiments. The sites selected were either low in organic matter, sandy in texture and/or in areas 

where apparent S deficiency had been reported. At each location, gypsum was applied at a rate to supply 50 lb S/ac prior to the final tillage operation before planting. Whole plant samples were collected at the sixth-leaf stage of growth and ear leaf samples were …