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Oxyrase And Pyruvate Inclusion In Extenders For Cold Stored Stallion Spermatozoa, Jordan T. Shore
Oxyrase And Pyruvate Inclusion In Extenders For Cold Stored Stallion Spermatozoa, Jordan T. Shore
MSU Graduate Theses
A current method in addressing subfertility in stallions include manipulating the semen extender media by supplementing antioxidants and energy sources. Two experiments (EXP 1/EXP 2) were conducted to assess the effects of pyruvate and Oxyrase® suspended in commercial diluents for preservation of stallion spermatozoa. Assessment of total (TM) and progressive motility (PM), velocity and direction of movement (VAP, VSL, VCL, and elongation) were recorded by CASA. In EXP 1, 3 different ejaculates were collected from each of 4 stallions. Aliquots of each ejaculate were suspended into 4 treatments of INRA96 with or without Oxyrase® supplemented at 2.4 U/ml …