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Expression And Characterization Of Alkaline Β-Glucosidase In Pichia Pastoris From Bacillus Pumilus, Kan Jinsong, Zhang Min, Xu Tao
Expression And Characterization Of Alkaline Β-Glucosidase In Pichia Pastoris From Bacillus Pumilus, Kan Jinsong, Zhang Min, Xu Tao
Food and Machinery
Engineered Pichia pastoris strain secreting and expressing β-glucosidase gene of Bacillus pumilus was constructed and enzymatic properties of recombinant enzyme were investigated. Based on the codon usage of Pichia pastoris, the codon of the β-glucosidase gene of Bacillus pumilus was optimized, the whole gene sequence was designed and synthesized, and the expression vector was constructed and transferred into Pichia pastoris. The results showed that the β-glucosidase gene of Bacillus pumilus had been successfully transferred into the yeast and secreted and expressed. The activity of the fermentation broth after 72 h induction could reach 25.39 U/mL. The optimum temperature and pH …