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Changsha University of Science and Technology

2019

ARTP; Saccharomyces cerevisiae; acid production; alcohol production

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Full-Text Articles in Agriculture

Breeding Of Saccharomyces Cerevisiae High-Yield Of Alcohol And Acid By Atmospheric Room Temperature Plasma, Wang Liye, Wang Haocheng, Ma Shan, Liu Weibing, Li Zehan, Song Jingjing, Yang Huafeng, Zhang Ying, Wu Yun May 2019

Breeding Of Saccharomyces Cerevisiae High-Yield Of Alcohol And Acid By Atmospheric Room Temperature Plasma, Wang Liye, Wang Haocheng, Ma Shan, Liu Weibing, Li Zehan, Song Jingjing, Yang Huafeng, Zhang Ying, Wu Yun

Food and Machinery

The Y6 strain screened from the grape surface was subjected to atmospheric pressure room temperature plasma (ARTP) mutagenesis, and the Saccharomyces cerevisiae strain with strong alcohol production ability, high acid production ability and stable fermentation performance were selected. The single-factor test confirmed that the optimal mutagenesis condition was lethality rate at 98.70% treated for 100 s. The target strain Y6-8 producing alcohol and producing acid was screened by 2,3,5-triphenyltetrazolium chloride (TTC) method, bromocresol green medium method and Du's tubule, and then its genetic stability was studied. The results showed that the mutagenized strain Y6-8 was …