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Secondary Metabolism Inducing Treatments During In Vitro Development Of Turmeric (Curcuma Longa L.) Rhizomes, Matthew M. Cousins, Jeffrey W. Adelberg, Feng Chen, James Rieck
Secondary Metabolism Inducing Treatments During In Vitro Development Of Turmeric (Curcuma Longa L.) Rhizomes, Matthew M. Cousins, Jeffrey W. Adelberg, Feng Chen, James Rieck
Jeffrey W Adelberg
Turmeric (Curcuma longa L.) plants that were grown in vitro for 17 or 22 weeks as a fed-batch culture in 2.5 L vessels yielded 39 to 43 g and 62 to 70 g of fresh rhizomes per vessel, respectively (95 % confidence interval). The MS liquid medium was maintained at 6 % sucrose through media addition twice during the experiment. Various methods were employed in attempts to increase secondary metabolism. Antioxidant and total phenolics assays were employed to characterize phytochemical activity. A first experiment exposed four clones to phenylalanine and/or methyl jasmonate (MeJa) from week 12 to 17 in culture. …
Short-Term And Long-Term Time Course Studies Of Turmeric (Curcuma Longa L.) Microrhizome Development In Vitro, Jeffrey Adelberg, Matthew M. Cousins
Short-Term And Long-Term Time Course Studies Of Turmeric (Curcuma Longa L.) Microrhizome Development In Vitro, Jeffrey Adelberg, Matthew M. Cousins
Jeffrey W Adelberg
Turmeric (Curcuma longa L.) plantlets were cultured in MS (Murashige and Skoog Physiol Plant 15:473–497, 1962) liquid medium with 6% sucrose. Microrhizome development was observed in the presence of methyl jasmonate (MeJa) (0, 5 and 16 μM) and benzyladenine (BA) (0, 0.32 and 1 μM). Leaf, root, rhizome growth, and sugar use were measured weekly for 6 weeks in small vessels (180 ml) and four times in 23 weeks in larger vessels (2.5 l). MeJa reduced leaf, root, and rhizome biomass. BA had a positive effect on biomass accumulation. Microrhizome mass increased at a linear rate during 6 weeks of …