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Full-Text Articles in Agriculture
Unitized, Nonselective Cutting Of In Vitro Watermelon, Jeff W. Adelberg, Y Alper, R E. Young, B B. Rhodes
Unitized, Nonselective Cutting Of In Vitro Watermelon, Jeff W. Adelberg, Y Alper, R E. Young, B B. Rhodes
Jeffrey W Adelberg
Unitized, nonselective mass cutting of Stage II Citrullus lanatus cv. Charlee (watermelon) plant tissue cultures has been achieved with various configurations and sizes of wire cutter devices. Time studies revealed that the wire cutters increased the productivity of the cutting function over conventional scalpel and forceps by a factor of 14, Total transfer productivity, including the manual functions of removing tissue from an initial culture vessel and filling (sorting and placing cut tissue segments) new vessels, was increased by a factor of 1,8, The square grid-type cutting devices yielded from 48 to 59% as many viable bud clusters per culture …
Approaching Mechanization Of Plant Micropropagation, Jeffrey Adelberg, R E. Young, A Hale, N D. Camper
Approaching Mechanization Of Plant Micropropagation, Jeffrey Adelberg, R E. Young, A Hale, N D. Camper
Jeffrey W Adelberg
Investigations of materials and methods for growing plant tissue in a continual-flow, liquid nutrient medium as an alternative to semisolid agar medium have been made. Enhanced growth of plant tissue on microporouspolypropylene membranes floating on a liquid nutrient has been demonstrated. Moreover, in vitro plantlets on the microporous membrane are free from entanglement with the support matrix and readily available to mechanizedhandling. Trained growth of plantlets through polypropylene netting shows potential for mechanization by mass handling (separation, singulation, and transfer) of plant tissue cultures.
Mass Handling Of Watermelon Microcuttings, Jeffrey Adelberg, Y Alper, R E. Young, B B. Rhodes
Mass Handling Of Watermelon Microcuttings, Jeffrey Adelberg, Y Alper, R E. Young, B B. Rhodes
Jeffrey W Adelberg
Modifications were made in the configurations of the unitizing, nonselective wire cutters used by Alper et al.(1992) for mass cuttings of Stage II Citrullus lanatus cv. Charlee (watermelon) plant tissue cultures to further enhance productivity. Mounting the cutter in an inverted position over the receiving vessel eliminated time required for filling. This cut-and-dump technique became 4.8 times more productive for the total transfer process than the conventional scalpel and forceps technique when both time and yield of cut segments with visible buds were considered. A concept for growing fewer, larger tissue clusters per vessel in mini-trays with orienting cells and …