Efficacy Of Biopesticides For Organic Management Of Cucumber Beetles, Mary A. Rogers

Doctoral Dissertations

Organic growers are limited in crop protection techniques for cucumber beetle management. Spotted (Diabrotica undecimpunctata howardi) and striped (Acalymma vitatta) cucumber beetles and are significant pests of cucurbits in the U.S. Feeding results in aesthetic damage and reduction in marketable yields as well as transmission of bacterial wilt that can result in plant mortality. Biopesticides are products formulated from naturally occurring organisms such as fungi and bacteria that are pathogenic or toxic to insect pests. Advantages to these products are that they have low environmental risk, low risk to non-target organisms including mammals and beneficial insects, …

Mortality Assessment Of Redwood And Mixed Conifer Forest Types In Santa Cruz County Following Wildfire, Steve R. Auten

Master's Theses

On August 12, 2009, the Lockheed Fire ignited the west slope of the Santa Cruz Mountains burning approximately 7,819 acres. Foresters and other land managers were left with challenging decisions on how to evaluate tree mortality. Big Creek Lumber Company, California Polytechnic State University, San Luis Obispo (Cal Poly)’s Swanton Pacific Ranch (SPR), and other resource professionals familiar with this region teamed up to develop a method for evaluating damage and thereby mortality for redwood, California nutmeg, live oak, tanoak, California bay, Pacific madrone, big leaf maple, Douglas-fir, Monterey pine, and knobcone pine. Quantitative damage criteria were used to design …

Cadmium Accumulation And Distribution In Lettuce And Barley, Fardausi Akhter

Electronic Thesis and Dissertation Repository

Cadmium (Cd) is a non-essential trace element and its environmental concentrations are increasing due to human activities. Edible plants can accumulate high concentrations of Cd, which could be toxic to humans. Understanding how and where Cd is stored in plants is important for ensuring lower concentration of Cd in the food. In this thesis, the accumulation and distribution of Cd in three agricultural plants, namely lettuce (Lactuca sativa L.), barley (Hordeum vulgare L.) and radish (Raphanus sativus L.), were investigated with a focus on the potential mechanisms involved in the localization of Cd in the root. The …

Protein Body Formation In Stable Transgenic Plants Of Nicotiana Tabacum Expressing Elastin-Like Polypeptide And Hydrophobin Fusion Proteins, Sonia P. Gutierrez

Electronic Thesis and Dissertation Repository

Plants are recognized as an efficient and inexpensive system to produce valuable recombinant proteins. However, the use of plants still faces two main limitations: the low accumulation levels of some recombinant proteins and the lack of efficient protein purification methods. Two fusion partners, elastin-like polypeptides (ELP) and hydrophobin I (HFBI) were found to increase the accumulation of recombinant proteins and induce the formation of protein bodies (PBs) when targeted to the ER in transient expression assays. In this study I examined the effect of these tags in stable transgenic plants of two Nicotiana tabacum cultivars when fused to green fluorescent …

Hairy Roots As A Model To Investigate The Role Of Suberin In The Phytophthora Sojae-Soybean Pathosystem, Pooja Sharma

Electronic Thesis and Dissertation Repository

Part of the resistance mechanism of soybean (Glycine max [L.] Merr.) to Phytopthora sojae Kauf. & Gerd. involves pre-formed root suberin. In order to investigate the role of suberin in this host-pathogen interaction, I characterized hairy roots, formed as a result of Agrobacterium rhizogenes (Riker et al.) Conn infection, as a model to be used as a reliable soybean transformation system. I established hairy root cultures and demonstrated that they were a result of A. rhizogenes infection. The anatomy and suberin deposition in soybean hairy roots was examined, and found to be very similar to that of wild-type roots. …

High School Horticulture Curriculum, Margaret Maratsos

Horticulture and Crop Science

Horticulture is just one of the many topics covered in a high school agriculture curriculum, and yet, there are very few lesson plans or resources available to teachers for these classes specifically. The objectives of this project were to compile a set of lesson plans, lab plans, and tests that would emphasize interactive and investigative learning. The lesson plans were written in such a way that they reflected a certain set of standards, set down by the state of California, and were meant to serve as a bare outline of topics that would be discussed within a week’s worth of …

The Impact Of Selected Forage Legumes On Cattle Performance, Forage Production, And Soil Quality, And Evaluation Of Legume Persistence Under Grazing, Bradley Edward Briggs

Graduate Theses and Dissertations

Interest in substituting legumes for N fertilizer in beef cattle grazing systems has recently increased with rising fertilizer prices. Legumes are well known for their ability to fix atmospheric N and decrease dependence on input of N fertilizer. However, there are still difficulties associated with legume utilization including establishment and persistence. Two experiments were conducted to evaluate legume performance under herbivory. The objective of Experiment 1 was to compare forage production and beef cattle gains from annual ryegrass [Lolium multiflorum (L.)] and bermudagrass [Cynodon dactylon (L.) Pers.] pastures fertilized with N or overseeded with legumes. Gelbvieh × Angus …

Cholesterol Lowering Effects Of Milk With Added Phytosterols, Laura Kells Cusack

Master's Theses

Foods incorporating plant sterols (PS) reduce Low-density lipoprotein (LDL) on average approximately 10%. PS with a higher lipid solubility may promote greater reductions. We examined the cholesterol lowering effect of a novel triglyceride recrystallized phystosterol (TRP). Twenty subjects (mean ± SD; age, 56 ± 10 years; BMI, 27 ± 5) with elevated LDL (>100 mg/dL) participated in three 4-week phases; Phase I, 2% milk; Phase II, milk with 2.0 grams (g) free PS; Phase III, milk with 2.0 g TRP. Before and after each phase two fasting blood draws were obtained for determination of serum cholesterol. Between the 2% …

An Investigation Of Fraxinus Americana Branch Sway Using A 3 Dimensional Motion Capture System, Anna T. Campiformio

Master's Theses

Abstract

Wind is a dominant abiotic factor that a tree experiences throughout its lifetime and can cause severe tree damage, resulting in risk of injury to humans, and economic and ecological losses. It is thought that trees develop structural properties and architectures that help withstand loading conditions by dissipating wind energy through damping mechanisms. The role of branch motion in reducing potential dangerous wind loads has been the focus of relatively few studies. Even fewer studies have examined tree sway response to natural wind loaded conditions. In this investigation, branch frequencies were calculated for three Fraxinus americana using a three-dimensional …

Multifunctionality In U.S. Rice Production: A Logit Analysis In Farmers Participation, Juan Tur Cardona

Graduate Theses and Dissertations

Multifunctional agriculture is particularly fundamental to some working lands conservation policies and programs, such as the Environmental Quality Incentive Program (EQIP), Conservation Security Program (CSP) and Wildlife Habitat Incentive Program (WHIP). Farmers can also be engaged in providing recreational and agri-tourism services such as hunting, fishing, bird-watching, farm tours, petting zoos and hospitality services. Using the Agricultural Resource Management Survey (ARMS) we analyze factors associated with participation in conservation, recreation and agri-tourism activities as a function of farm structure, farm financial measures, production practices, and socio-demographic characteristics of the farm operator. To estimate the functional relationships we estimate a binary …

Chemical Responses Of Two Deciduous Trees After 10-Years Of Exposure To Free Air Co2 Enrichment, Keonhee Kim

Masters Theses

Globally, the mean atmospheric carbon dioxide level has risen steadily since pre-industrial times, which is largely attributable to human activities such as increased emissions from fossil fuel burning, and clearing of forests. Numerous research efforts have been made to understand responses of trees in higher atmospheric carbon dioxide levels in short term, small-scale experiments. To overcome the limitations of these experiments and understand the response of trees in a natural environment, the Free Air Carbon dioxide Enrichment (FACE) project was proposed. FACE is a global project that involves experimentally enriching carbon dioxide in the atmosphere of terrestrial ecosystems. The FACE …

Prevalence Of Copper Resistance Among Foliar Bacterial Pathogens Of Tomato In Tennessee, Jonathon Thomas Mixon

Masters Theses

Foliar bacterial diseases are among the most important diseases of tomato. Bacterial spot is caused by four species: Xanthomonas euvesicatoria, X. vesicatoria, X. perforans, and X. gardneri, hereafter referred to as Xanthomonas spp. Bacterial speck is caused by Pseudomonas syringae pv. tomato and bacterial canker is caused by Clavibacter michiganensis subsp. michiganensis. Fixed copper products are relied upon extensively for control, due to a lack of effective and economical alternatives. Copper resistance in bacterial spot and speck pathogens has been reported worldwide. Copper resistance quite likely exists in Tennessee, but the extent has never been …

Strawberry Growth, Yield, Fruit Nutrition, And Control Of Verticillium Wilt With Pre-Plant Soil Fumigants, Ozone, And Biological Control, Justin J. Scurich

Master's Theses

Verticillium wilt is a widespread soilborne disease of strawberry historically controlled by soil fumigation with methyl bromide (MB). MB was banned by the United Nations in 1995 and will be completely phased out by 2015. Research has concentrated on alternative methods of disease control without finding a single alternative able to replace MB in widespread disease control and yield increase. For the current study, strawberries were greenhouse grown in container pots filled with soil from both infested and non-infested areas of a commercial strawberry field in Watsonville, CA. Treatments included pre-plant soil fumigation with commercially available formulations of methyl bromide, …

Potential For Barn Owl As Rodent Biological Control In Central California Vineyards, Hannah Tillmann

Biological Sciences

The pocket gopher (Thomomys bottae) and voles (Microtus spp.) cause significant economic damage to vineyards. In response, many growers have taken steps to attract Barn Owls (Tyto alba) to their properties to help keep these rodent populations in check. This research project investigated Barn Owl consumption of pocket gophers and voles in Central California vineyards in order to assess the efficiency of this integrated pest management strategy. I collected a total of 715 owl pellets from five vineyard locations in Templeton and Paso Robles, California over an eight-month period during nesting and post-fledging seasons. I …

Evaluation Of Heat Shock Protein 70a (Hsp70a) In Chlamydomonas Reinhardtii, Sarah Nicole Short

Theses and Dissertations--Biosystems and Agricultural Engineering

Algae are being considered as a possible tool for carbon dioxide mitigation because they uptake carbon dioxide during photosynthesis. Using flue gas from a coal-fired power plant as a carbon source would allow the algae to remove CO₂ from the flue gas before it is emitted into the atmosphere. Because algae do not grow well at the high temperature, low pH conditions presented by flue gas, the traditional approach has been to alter the flue gas to suit the needs of the algae; however, this work aimed to genetically modify the algae Chlamydomonas reinhardtii to grow better at less …

Soil Microbial Community Response To Climate Change: Results From A Temperate Kentucky Pasture, Lindsey C. Slaughter

Theses and Dissertations--Plant and Soil Sciences

Climate change is likely to alter plant species composition and interactions between plants and soil microbes that together dictate the quantity and quality of forage produced in pastures, the base of animal production in central Kentucky. This study assessed the seasonal dynamics of soil microbes and their response to increased temperature (+3^oC) and growing season precipitation (+30% of the mean annual). Total soil microbial biomass, community composition, enzyme activities, potential carbon mineralization, and catabolic responses to selected substrates were measured seasonally in the different climate treatments. In this system, seasonal variability was a dominant driving factor for all …

Enantioselective Demethylation: The Key To The Nornicotine Enantiomeric Composition In Tobacco Leaf, Bin Cai

Theses and Dissertations--Plant and Soil Sciences

Nicotine and nornicotine are the two main alkaloids that accumulate in Nicotiana tabacum L. (tobacco), and nornicotine is the N-demethylation metabolite of nicotine. Nicotine is synthesized in the root, and probably primarily in the root tip. Both nicotine and nornicotine exist as two isomers that differ from each other by the orientation of H atom at the C-2' position on the pyrrolidine ring. (S)-nicotine is the dominant form in tobacco leaf and the enantiomer fraction of nicotine (EF_nic), the fraction of (R)-enantiomer over the total nicotine, is approximately 0.002. Despite considerable efforts to elucidate nicotine and nornicotine …

Using An Active Optical Sensor To Improve Nitrogen Management In Corn Production, Donato Titolo

Theses and Dissertations--Plant and Soil Sciences

Corn nitrogen (N) applications are still done on a field basis in Kentucky, according to previous crop, soil tillage management and soil drainage. Soil tests, as well as plant analysis for N, are not very useful in making N fertilizer rate recommendations for corn. Recommended rates assume that only 1/3 to 2/3 of applied N is recovered, variability largely due to the strong affect of weather on the release of soil N and fertilizer N fate. Many attempts have been made to apply N in a more precise and efficient way. Two experiments were conducted at Spindeltop, the University of …