Agriculture Commons^™

Towards The Quantification Of Estrone And 17Β-Estradiol Conjugates In Dairy Cattle Urine Sorptive Stir Bar Extraction And Gas Chromatography-Mass Spectometry, S.M. Istiak Ahmed

Masters Theses & Specialist Projects

The global concern regarding the presence of compounds with estrogenic properties in the environment has grown significantly. Endocrine-disrupting compounds (EDCs) are environmental pollutants that disrupt the endocrine systems of both wildlife and humans. Estrogens, with their potent estrogenic effects, are particularly noteworthy among the various substances with endocrine-disrupting properties. They are of concern due to their prevalence in the environment, especially due to dairy waste contamination. These lipophilic compounds can accumulate in livestock fat tissues, potentially entering the human food chain. Sulfated forms of estrone and β- estriol, which exhibit a higher degree of saturation than their non-conjugated counterparts, have …

Liquid Chromatography Mass Spectrometry Method Development For The Determination Of Β–Defensins In Bovine Milk, Elisha Ogbebor

Masters Theses & Specialist Projects

Bovine mastitis is caused by a wide range of pathogens which results in a substantial economic loss for the dairy cattle livestock industry. β-defensins are a part of the innate immune system and act as the first line of defense against mastitis in bovine. β-defensins (~6- 10kDa) are antimicrobial peptides that contain about 28 to 67 amino acids, the presence of six cysteine residues results in their three disulfide bonds and a β-sheet structure. A few β- defensins have been identified in cows based on the site of occurrence and time of expression in tissue, but not in milk. A …

Liquid Chromatography With Tandem Mass Spectrometry Method Development For The Determination Of Β–Defensins In Bovine Milk, Symone T. Whalin

Masters Theses & Specialist Projects

Bovine mastitis, caused by a wide array of pathogens, results in a substantial economic loss for the dairy cattle livestock industry. β-defensins are a part of the bovine’s innate immune system and act as the first line of defense against mastitis. Only foundational research has been done on β-defensins’ ability to treat and prevent mastitis. There have been no analytical methods reported in the literature for analyzing β-defensins in bovine milk. This research aims to create an analytical approach to determine β-defensins in bovine milk. It is challenging to determine an analyte in a complex sample matrix, and milk is …

Quantification Of Tylosin Antibiotics In Cattle Waste, Appala Keerthi

Masters Theses & Specialist Projects

Antibiotics are used as prophylactic agents to promote growth and for treating infections in animals. However, the irrational use of antibiotics in livestock management is a significant cause of the development of antibioticresistant genes in the environment. Each year 2 million people suffer from the infections caused by bacteria which are resistant to antibiotics and 23,000 of these people are estimated to die because of antibiotic resistance. New drugs are continually coming into the market but are at the risk of developing resistance. Thus, there is a need for the development of analytical methods which can be used to monitor …

Agricultural Contaminant Source And Transport In A Karst Groundwater Basin, Patricia Kambesis

Masters Theses & Specialist Projects

Agricultural land use in areas that are located in karst groundwater basins negatively impact groundwater quality because karst terrains provide multiple, direct hydrologic connections from the surface into karst aquifers. The connections and rapid velocities associated with surface and subsurface flow in karst aquifers allow for contaminants to move quickly into and through a groundwater basin. When groundwater returns to the surface via a spring or springs, any contaminants within the water become part of surface streams and rivers. These in turn, impact water quality in areas located downstream of the spring or springs. The purpose of this study was …

Evaluation Of Continuous Flow Constructed Wetlands Treating Swine Waste, Stephanie Rivard

Masters Theses & Specialist Projects

The ability for wetlands to purify contaminated water is not a new concept. Natural wetlands have been cleansing water within our environment for ages. After studying the process of natural wetlands the same concept was applied to municipal waste. It is the success of constructed wetlands treating municipal waste that created the new idea for the application of a wetland system to treat wastewater from livestock facilities. Through proper design and management, constructed wetlands may be useful for reducing the nutrient concentration of waste (Rieck el al., 1996). Taking into consideration the higher nutrient loads of livestock waste, the same …

Evaluation Of Constructed Wetlands For The Waste Management Of A Large Scale Swine Production Unit, Robert Sutton

Masters Theses & Specialist Projects

The effectiveness of using constructed wetlands to remove unwanted nutrients, increase dissolved oxygen while at the same time decreasing the biological oxygen demand, and to reduce the levels of the Fecal Coliform Bacteria from a swine operation was evaluated. The indicator of proper waste purification will be the result of testing for the following: ammonia nitrogen, nitrate nitrogen, total phosphorus, total suspended solids, dissolved solids, dissolved oxygen, biological oxygen demand, and Fecal Coliform Bacteria. The wetland was divided into nine connected cells that covered approximately 3.8 hectares. Material was loaded from an anaerobic holding lagoon on four separate occasions during …

Physical Properties Of Tar Sand Tailings That Influence Their Ability To Sustain Plant Growth, Timothy Cole

Masters Theses & Specialist Projects

The mining of bituminous sandstone rock for the purpose of extracting bitumen has created a need for reclamation. One such mine is located in northern Logan County. Their mining operation involves removing large sections of sandstone, which is impregnated with bitumen, and crushing it to sand-sized material. The sand-sized material is then mixed with organic solvents which extract the bitumen. Once separated, the bitumen is stored for future refinement and the spent sand is stockpiled for later disposal.

The spent sands or waste material are referred to as tar sand tailings. These tailings have particular properties which make reclamation efforts …