Agriculture Commons^™

Ecology, Behavior And Bionomics First Genotyping Of Spodoptera Frugiperda (J. E. Smith) (Lepidoptera: Noctuidae) Progeny From Crosses Between Bt-Resistant And Bt-Susceptible Populations, And 65-Locus Discrimination Of Isofamilies, Renee S. Arias, Maribel Portilla, J. D. Ray, Carlos A. Blanco, S. A. Simpson, Brian E. Scheffler

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Concerns exist that Bt-resistant populations of Spodoptera frugiperda (J. E. Smith) (Lepidoptera: Noctuidae) found in Puerto Rico, could spread to continental United States, and about the lack of molecular tools to monitor potential crosses or distinguish populations. In this work, the feasibility of genotyping S. frugiperda crosses between Bacillus thuringiensis (Berliner) (Bacillales: Bacillaceae) (Bt) resistant and susceptible populations using simple sequence repeats (SSRs, or microsatellites) was assessed. Parents and their corresponding progeny (five resistant, five susceptible phenotype) were genotyped using 192 SSRs on three reciprocate crosses alternating male and female from Bt-susceptible and Bt-resistant populations. Oviposition, mortality and fecundity were …