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Full-Text Articles in Agriculture
High Throughput Genomic Sequencing Of Bioaerosols In Broiler Chicken Production Facilities, Kate M. O’Brien, Michael S. Chimenti, Morgan Farnell, Tom Tabler, Thomas Bair, Joey Lynn Bray, Matthew W. Nonnenmann
High Throughput Genomic Sequencing Of Bioaerosols In Broiler Chicken Production Facilities, Kate M. O’Brien, Michael S. Chimenti, Morgan Farnell, Tom Tabler, Thomas Bair, Joey Lynn Bray, Matthew W. Nonnenmann
Faculty Publications
Chronic inhalation exposure to agricultural dust promotes the development of chronic respiratory diseases among poultry workers. Poultry dust is composed of dander, chicken feed, litter bedding and microbes. However, the microbial composition and abundance has not been fully elucidated. Genomic DNA was extracted from settled dust and personal inhalable dust collected while performing litter sampling or mortality collection tasks. DNA libraries were sequenced using a paired-end sequencing-by-synthesis approach on an Illumina HiSeq 2500. Sequencing data showed that poultry dust is predominantly composed of bacteria (64–67%) with a small quantity of avian, human and feed DNA (< 2% of total reads). Staphylococcus sp. AL1, Salinicoccus carnicancri and Lactobacillus crispatus were the most abundant bacterial species in personal exposure samples of inhalable dust. Settled dust had a moderate relative abundance of these species as well as Staphylococcus lentus and Lactobacillus salivarius. There was a statistical difference between the microbial composition of aerosolized and settled dust. Unlike settled dust composition, aerosolized dust composition had little variance between samples. These data provide an extensive analysis of the microbial composition and relative abundance in personal inhalable poultry dust and settled poultry dust.
Exposure Of Workers To Dust And Bioaerosol On A Poultry Farm, Sheryll B. Jerez, Y. Cheng, Joey Bray
Exposure Of Workers To Dust And Bioaerosol On A Poultry Farm, Sheryll B. Jerez, Y. Cheng, Joey Bray
Faculty Publications
Poultry houses are known for generating excessive dust, which originates from bedding materials, fiberglass insulations, feed, dried fecal materials, and feather particles. Dust may contain microorganisms, including endotoxins, fungi, and bacteria, that may affect living things when inhaled. Dust that contains living organisms is referred to as bioaerosol, and its particle size may range from 0.5 to 100 µm. Respirable dust, which has an aerodynamic diameter of less than or equal to 4 µm, can travel to and be deposited in the gas-exchange region of the human respiratory system. This is of particular concern because of the greater health hazard …
Measurement Of Particle Size Distribution In A Swine Building, Sheryll B. Jerez, Yuanhui Zhang, X Wang
Measurement Of Particle Size Distribution In A Swine Building, Sheryll B. Jerez, Yuanhui Zhang, X Wang
Faculty Publications
The majority of the research in animal buildings has been on measured concentrations of contaminants that the workers and animals are exposed to; emission measurements have only gained attention in recent years due to potential federal regulations on air quality emissions from animal feeding operations (AFOs). The contribution of AFOs to ambient PM10 and PM2.5 entails reliable measurement of particle size distribution. The objective of this study was to measure and compare the size distribution of particulate matter (PM) at multiple locations inside and at the exhausts of a wean‐to‐finish commercial swine building. The particle size distribution was measured by …
Spatial And Temporal Distributions Of Dust And Ammonia Concentrations In A Swine Building, Sheryll B. Jerez, Yuanhui Zhang, X Wang
Spatial And Temporal Distributions Of Dust And Ammonia Concentrations In A Swine Building, Sheryll B. Jerez, Yuanhui Zhang, X Wang
Faculty Publications
Pollutants, especially dust, are rarely uniformly distributed within ventilated air spaces due to non‐uniform flow fields, particle inertia, gravitational settling, and diffusion. Thus, selecting suitable sampling locations for representative sampling is a challenge. The objective of this study was to determine the spatial and temporal distributions of dust and ammonia concentrations(NH3) in a swine building. Results of this study are useful in the design of sampling strategies that require limited sampling locations and in studying pollutant transport. This study was conducted in a commercial swine building in Illinois. The total suspended particulate (TSP) matter and ammonia concentrations were measured at …