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Intsormil Crsp: Sorghum, Millet And Other Grains Collaborative Research Support Program: Leader With Associates Cooperative Agreement No. Eep-A-00-06-0016-00; Award From The U.S. Agency For International Development To The University Of Nebraska-Lincoln, Joseph Schmidt

INTSORMIL Scientific Publications

Pursuant to the authority contained in the Foreign Assistance Act of 1961, as amended, the U.S. Agency for International Development (USAID) hereby awards to University of Nebraska - Lincoln (hereinafter referred to as the "Recipient"), the sum of $9,000,000.00 to provide support for a program in Sorghum, Millet, and Other Grains Collaborative Research Support Program as described in the Schedule of this award and in Attachment B, entitled "Program Description."

This Leader with Associates Cooperative Agreement is effective and obligation is made as of 09-30-2006 and shall apply to expenditures made by the Recipient in furtherance of program objectives during …

Proceedings Of The 33rd Annual Meeting, Southern Soybean Disease Workers (March 8-9, 2006, Jackson, Tennessee), Michael E. Schmidt, Ray Schneider, Daniel H. Poston, Jason Bond, Steve R. Koenning

Southern Soybean Disease Workers: Conference Proceedings

Contents

Southern Soybean Disease Workers 2005 treasury report

Southern United States Soybean Disease Loss Estimates for 2005. Compiled by SR Koenning

Virulence in Phytopbthora sojae Isolates to Soybeans with Rps8 Resistance. DA Smith, TS Abney, and JG Shannon

New Sources of Resistance to SCN in Soybean. PR Arelli

Soybean Disease Management in Louisiana. B Padgett, MA Purvis, and BW Garber

Efficacy and Profitability of Foliar Fungicide in the Absence of Soybean Rust. M Newman and W Percell

Fungicide and Insecticide Combinations for Enhancing Soybean Health and Yield. RP Mulrooney and RW Taylor

Overview of Soybean Rust Monitoring in the US. …

Effects Of Substrate And Temperature On Growth Of Aspergillus Flavus In Peanuts From Georgia, Premila Achar, Andres Sachez

Faculty and Research Publications

We investigated the effects of different substrates (potato dextrose agar [PDA], nutrient agar [NA], and corn meal agar [CMA]) and temperatures on growth and aflatoxin production in Aspergillus flavus. Contaminated peanuts from retailers at different locations in Georgia were used throughout the experiment and 200 seeds were selected from each sample. PDA, NA, and CMA served as the substrate to express the mold from the contaminated seeds. Seeds were plated equidistantly on each of the media and incubated at 10° C, 27° C, 30° C, and 37° C, respectively. Seeds incubated on moist filter paper served as control. Neither growth …

Evaluation Of The Single Kernel Characterization System (Skcs) For Measurement Of Sorghum Grain Attributes, S. R. Bean, O. K. Chung, M. R. Tuinstra, Jeffrey F. Pedersen, J. Erpelding

Department of Agronomy and Horticulture: Faculty Publications

The single kernel characterization system (SKCS) has been widely used in the wheat industry, and SKCS parameters have been linked to end-use quality in wheat. The SKCS has promise as a tool for evaluating sorghum grain quality. However, the SKCS was designed to analyze wheat, which has a different kernel structure from sorghum. To gain a better understanding of the meaning of SKCS predictions for grain sorghum, individual sorghum grains were measured for length, width, thickness (diameter), and weight by laboratory methods and by the SKCS. SKCS predictions for kernel weight and thickness were highly correlated to laboratory measurements. However, …

Chemical Composition And Response To Dilute-Acid Pretreatment And Enzymatic Saccharification Of Alfalfa, Reed Canarygrass, And Switchgrass, Bruce S. Dien, Hans-Joachim G. Jung, Kenneth P. Vogel, Michael D. Casler, Joann F. S. Lamb, Loren Iten, Robert C. Mitchell, Gautum Sarath

Department of Agronomy and Horticulture: Faculty Publications

Alfalfa stems, reed canarygrass, and switchgrass; perennial herbaceous species that have potential as biomass energy crops in temperate regions; were evaluated for their bioconversion potential as energy crops. Each forage species was harvested at two or three maturity stages and analyzed for carbohydrates, lignin, protein, lipid, organic acids, and mineral composition. The biomass samples were also evaluated for sugar yields following pretreatment with dilute sulfuric followed by enzymatic saccharification using a commercial cellulase preparation. Total carbohydrate content of the plants varied from 518 to 655 g kg^-1 dry matter (DM) and cellulose concentration from 209 to 322 g kg …

Mutations In Arabidopsis Yellow Stripe-Like1 And Yellow Stripe-Like3 Reveal Their Roles In Metal Ion Homeostasis And Loading Of Metal Ions In Seeds, Brian M. Waters, Heng-Hsuan Chu, Raymond J. Didonato, Louis A. Roberts, Robynn B. Eisley, Brett Lahner, David E. Salt, Elsbeth L. Walker

Department of Agronomy and Horticulture: Faculty Publications

Here, we describe two members of the Arabidopsis (Arabidopsis thaliana) Yellow Stripe-Like (YSL) family, AtYSL1 and AtYSL3. The YSL1 and YSL3 proteins are members of the oligopeptide transporter family and are predicted to be integral membrane proteins. YSL1 and YSL3 are similar to the maize (Zea mays) YS1 phytosiderophore transporter (ZmYS1) and the AtYSL2 iron (Fe)-nicotianamine transporter, and are predicted to transport metal-nicotianamine complexes into cells. YSL1 and YSL3 mRNAs are expressed in both root and shoot tissues, and both are regulated in response to the Fe status of the plant. β-Glucuronidase reporter expression, driven by …

Soybean Cultivars Resulted From More Recombination Events Than Unselected Lines In The Same Population, T. R. Stefaniak, D. L. Hyten Jr., V. R. Pantalone, A. Klarer, T. W. Pfeiffer

Department of Agronomy and Horticulture: Faculty Publications

The selection of superior adapted cultivars has contributed to the doubling of soybean [Glycine max (L.) Merr.] yields in the USA since 1930. Genetic variation was required for this selection to be effective. The objective of this study was to evaluate the importance of homologous chromosome meiotic recombination in the creation of soybean cultivars. A set of 10 adapted high-yielding cultivars selected from the cross ‘Williams’ × ‘Essex’ was com-pared with a set of 156 random recombinant inbred lines (RILs) from the same population. Crossover events were identified using 143 simple sequence repeat (SSR) markers span-ning all 20 soybean …

Snp-Phage – High Throughput Snp Discovery Pipeline, Lakshmi K. Matukumalli, John J. Grefenstette, David L. Hyten, Ik-Young Choi, Perry B. Cregan, Curt P. Van Tassell

Department of Agronomy and Horticulture: Faculty Publications

Background: Single nucleotide polymorphisms (SNPs) as defined here are single base sequence changes or short insertion/deletions between or within individuals of a given species. As a result of their abundance and the availability of high throughput analysis technologies SNP markers have begun to replace other traditional markers such as restriction fragment length polymorphisms (RFLPs), amplified fragment length polymorphisms (AFLPs) and simple sequence repeats (SSRs or microsatellite) markers for fine mapping and association studies in several species. For SNP discovery from chromatogram data, several bioinformatics programs have to be combined to generate an analysis pipeline. Results have to be stored in …

Application Of Machine Learning In Snp Discovery, Lakshmi K. Matukumalli, John J. Grefenstette, David L. Hyten, Ik-Young Choi, Perry B. Cregan, Curtis P. Van Tassell

Department of Agronomy and Horticulture: Faculty Publications

Background: Single nucleotide polymorphisms (SNP) constitute more than 90% of the genetic variation, and hence can account for most trait differences among individuals in a given species. Polymorphism detection software PolyBayes and PolyPhred give high false positive SNP predictions even with stringent parameter values. We developed a machine learning (ML) method to augment PolyBayes to improve its prediction accuracy. ML methods have also been successfully applied to other bioinformatics problems in predicting genes, promoters, transcription factor binding sites and protein structures. Results: The ML program C4.5 was applied to a set of features in order to build a SNP classifier …

Impacts Of Genetic Bottlenecks On Soybean Genome Diversity, D. L. Hyten, Qijian Song, Youlin Zhu, Ik-Young Choi, Randall L. Nelson, Jose M. Costa, James E. Specht, Randy C. Shoemaker, Perry B. Cregan

Department of Agronomy and Horticulture: Faculty Publications

Soybean has undergone several genetic bottlenecks. These include domestication in Asia to produce numerous Asian landraces, introduction of relatively few landraces to North America, and then selective breeding over the past 75 years. It is presumed that these three human-mediated events have reduced genetic diversity. We sequenced 111 fragments from 102 genes in four soybean populations representing the populations before and after genetic bottlenecks. We show that soybean has lost many rare sequence variants and has undergone numerous allele frequency changes throughout its history. Although soybean genetic diversity has been eroded by human selection after domestication, it is notable that …

Ethylene Could Influence Ferric Reductase, Iron Transporter, And H+-Atpase Gene Expression By Affecting Fer (Or Fer-Like) Gene Activity, Carlos Lucera, Brian M. Waters, Francisco Javier Romera, María José García, María Morales, Esteban Alcántara, Rafael Pérez-Vicente

Department of Agronomy and Horticulture: Faculty Publications

In previous works, it has been shown, by using ethylene inhibitors and precursors, that ethylene could participate in the regulation of the enhanced ferric reductase activity of Fe-deficient Strategy I plants. However, it was not known whether ethylene regulates the ferric reductase gene expression or other aspects related to this activity. This paper is a study of the effects of ethylene inhibitors and precursors on the expression of the genes encoding the ferric reductases and iron transporters of Arabidopsis thaliana (FRO2 and IRT1) and Lycopersicon esculentum (= Solanum lycopersicum) (FRO1 and IRT1) plants. The effects …