Life Sciences Commons^™

The Effects Of Ammonia On The Functionality Of Bovine Granulosa Cells, Micah Henrie

All Graduate Theses and Dissertations, Fall 2023 to Present

It is common practice in the livestock animal production industries to feed high levels of protein to maximize the yield of each animal in production. However, diets high in protein can cause decreased reproductive success within these animals. The causes behind this decrease are not fully understood. Increased dietary protein leads to increased levels of both ammonia and urea in the blood of the animal. These increased metabolite levels may be a possible explanation for the decline in reproductive success. The goal of this study is to identify how various levels of ammonia affect the function of bovine granulosa cells …

Apomixis And The Reproductive Dynamics Of Eastern Gamagrass Tripsacum Dactyloides L., B Kindiger, C L. Dewald

IGC Proceedings (1997-2023)

Studies were conducted to determine the reproductive characteristics of Tripsacum, a perennial, native pasture species and to identify methods for breeding agronomically superior cultivars. Reproductive methods and types of individuals generated were evaluated with triploid and hexaploids possessing a novel method for transferring sexual germplasm into apomictic tetraploids and tetraploid germplasm into sexual diploids. Triploids and hexaploids are demonstrated to be useful materials for introgressing and transferring desirable alleles across ploidy levels and reproductive barriers.

Physiological Impact Of Ergot Alkaloid Consumption In Ruminant Livestock, J. L. Klotz, S. K. Duckett, D. L. Harmon

IGC Proceedings (1997-2023)

Ergot alkaloids in feeds and forages are a worldwide concern. Consumption of ergot alkaloids by ruminant livestock can range from extreme cases that threaten the life of the animal to more frequent and chronic outcomes where livestock productivity is decreased. Consumption of ergot alkaloids alters ruminant physiology such that it outwardly manifests in lower animal intake and gain, decreased reproductive efficiency, and a compromised circulatory system. This talk will cover current research that is improving our understanding of how ergot alkaloids alter cell and tissue physiology that results in the compromised growth and reproduction observed at the whole animal level.

On The Oviposition Of Homonota Aff. Darwinii In The Puna Region Of The Central Andes Of Argentina, Franco Valdez Ovallez, Rodrigo Gómez Alés, Rodrigo Nieva Cocilio, Tomás Martínez, Juan Carlos Acosta, José Villavicencio, Graciela Blanco

Turkish Journal of Zoology

Communal nest egg-laying refers to females laying their eggs with those of their conspecifics under or within artificial or natural structures. Homonota aff. darwinii is a recently discovered species that has not yet been formally described. The objective of this work is to characterise the oviposition of this species in the Puna region in the Central Andes of Argentina. We recorded the type and surface of the rocks where the eggs were laid, the temperature of the substrate, and the temperature of the rock surface facing the substrate. In addition, we recorded the daily temperature variation that a randomly selected …

Spawning Asynchrony Of The Endangered Acropora Cervicornis: Are Light Pollution And Abnormally Warm Temperatures The Culprits?, Krista Laforest

All HCAS Student Capstones, Theses, and Dissertations

The persistence of reefs relies on mature corals spawning synchronously to maximize fertilization and produce larvae to replenish local populations. Corals synchronize the release of gametes by responding to temperature, sun, and moon light cycles; however, abnormalities in these patterns can disrupt synchrony. This study is the first to describe regional asynchronous spawning of Acropora cervicornis by quantifying gamete development and spawning times among two reefs, an in situ nursery off Fort Lauderdale, and an in situ nursery in the Florida Keys. While A. cervicornis in the Florida Keys synchronously spawned within the predicted window of 2-5 days after the …

Impact Of Cadmium Stress On Soil Virus Reproduction And The Persistence Of Viruses Under Abiotic Conditions, Zhibo Cheng

Doctoral Dissertations

Soil viruses are ubiquitous and greatly impact the structure and function of soil microbial communities, with their effects modulated by various environmental factors. This study investigates the inactivation of naturally occurring soil viruses in sterilized soil, as well as the effects of cadmium (Cd) exposure and carbon (C) and nitrogen (N) nutrient availability on the population dynamics of virus-host communities in agricultural soil.

Lab-scale slurry and unsaturated experiments were performed to examine virus inactivation in the absence of host bacteria in sterilized soil. In slurry experiments, virus abundance declined by over 90% after 10 days of incubation. The addition of …

Assessing The Effects Of The Spring Hunting Season Start Date On Wild Turkey Seasonal Productivity And Hunter Behavior, Joseph Quehl

Masters Theses

Many states throughout the Southeast have documented declines in wild turkey (Meleagris gallopavo) harvest and estimates of recruitment in poult-per-hen ratios. Wild turkey populations are driven by seasonal productivity, so the decline in these parameters may indicate a decline in the overall population. One hypothesis as to why we are seeing a reduction in productivity and a potential population decline is that the spring hunting season is disrupting the reproductive cycle by harvesting too many males before they have had the opportunity to breed, or by harvesting dominant males and disrupting the social hierarchy of the flock. Our …

Evaluating The Role Of The Bovine Vaginal Microbiome In Neonatal And Maternal Health Outcomes, Riley D. Messman

Theses and Dissertations

The dam vaginal microbiota is the first major microbial inoculating community within the neonate. The composition of the dam vaginal microbiota has implications in calf commensal microbiota development. Alterations of the dam microbial community prior to parturition could alter inoculating communities and immune responses in both the dam and calf. Thus, authors aimed to elucidate the microbial community composition of the bovine dam vaginal and calf nasal microbiota post-partum after utilizing betadine lavages (BL). The dam vaginal and calf nasal microbial communities and immune responses were evaluated at 0-, 15-, 30- and 60-day post-partum. Microbiota composition of the dam haircoat, …

Effect Of Supplementation Prior To Artificial Insemination And During Gestation In Beef Females, Landon Tadich

Department of Animal Science: Dissertations, Theses, and Student Research

Objectives of Experiment 1 was to evaluate the impact of increased energy prior to AI on BW, ADG, and reproductive efficiency in heifers developed on range or in a dry lot. During the winter development period (131 ± 3.5 d/yr) heifers (n= 100/yr) were assigned to either upland range (RANGE) or fed a dry lot diet in two pens that either remained constant (DLLO) or increased in energy prior to AI (DLHI). Heifers developed on RANGE grazed upland winter range until 38 d prior to AI where they then received the DLLO diet. Pre-breeding BW was greater (P £ …

Mating Ewes On Condensed Tannin-Containing Forages Increases Ewe Reproductive Rate And Reduces Lamb Mortality, T. N. Barry, C. A. Ramirez-Restrepo, E. L. Mcwilliam, N. Lopez-Villalobos, P. D. Kemp

IGC Proceedings (1997-2023)

Action of condensed tannin (CT) reduces forage protein degradation in the rumen and increases the absorption of amino acids from the small intestine (Barry & McNabb 1999). This paper reports the effects of grazing ewes on two CT-containing forages during mating upon ewe reproductive rate and lamb mortality.

Detection And Observed Correlations Of Statin And Beta-Blocking Pharmaceuticals In West Virginia Surface Waters And Ichthyofauna, Joseph W. Kingsbury

Graduate Theses, Dissertations, and Problem Reports

Within this thesis the source, presence, and the estimated ecotoxicological effects of cardiovascular medicines, specifically statins and beta-blockers are described within the Buckhannon, Tygart Valley, and West Fork Rivers of West Virginia. Included within this thesis is a general introduction providing key background information and relevant research related to the source, presence, and known toxicity of statins and beta-blockers. Following the general introduction there are 3 distinct chapters. Chapter 1 focuses on the estimated demographic based source loadings and the surface water concentration characteristics of statins and beta-blockers within the three rivers. Chapter 2 investigates the effects of statins and …

Lifetime Inclusive Fitness Effects Of Cooperative Polygamy In The Acorn Woodpecker, Walter D. Koenig, Sahas Barve, Joseph Haydock, Eric L. Walters

Biological Sciences Faculty Publications

Although over 50 y have passed since W. D. Hamilton articulated kin selection and inclusive fitness as evolutionary explanations for altruistic behavior, quantifying inclusive fitness continues to be challenging. Here, using 30 y of data and two alternative methods, we outline an approach to measure lifetime inclusive fitness effects of cooperative polygamy (mate-sharing or cobreeding) in the cooperatively breeding acorn woodpecker Melanerpes formicivorus. For both sexes, the number of offspring (observed direct fitness) declined while the number of young parented by related cobreeders (observed indirect fitness effect) increased with cobreeding coalition size. Combining these two factors, the observed inclusive …

Parental Instincts: The Neurological And Biological Factors Associated With Parenthood, Jared Reeder

Undergraduate Honors Theses

The following project involves a systematic review of the scientific literature on neural and biological changes of mothers and fathers in parenthood. Until very recently, little scientific research was devoted to studying how bearing children affects a man or woman’s long-term biology. Over the last twenty years, studies of neuroplastic changes in new mothers show specific neural mechanisms responsible for altering the behaviors of mothers during and after pregnancy. These changes in neuroplasticity alter behavior in such a way that led to mothers requiring less sleep and being more prone to hearing the cries of their children. In addition to …

The Molecular Regulators, Pathways, And Environmental Impacts Of White Flowers, Sagheer Ahmad, Zaibun Nisa, Muhammad Zeeshan Munir, Muhammad Imran, Shaista Nosheen, Kai Zhao

Turkish Journal of Agriculture and Forestry

White flowers are an important element of natural beauty, although they are thought of colorless. Anthocyanins are the major compounds responsible for a variety of pigments in flowers. White flowers surely lack pigmented anthocyanins and other compounds, but there are regulatory mechanisms that hinder the deposition of color pigments in white flowers. Moreover, there are also some compounds that deposit to instigate white appearance and the genetic regulators control the biosynthesis and deposition of such compounds. Experts have shown that fluctuation in the equilibrium of FLS and DFR genes determines the degree of accumulation of anthocyanins and flavonols in the …

2023 Cattlemen’S Day Full Report, Liz Boyle

Kansas Agricultural Experiment Station Research Reports

This report includes research conducted in 2022 on beef cattle management, reproduction, nutrition, and meat science conducted at Kansas State University.

Quantifying Life History Energetics Of An Oviparous Elasmobranch Subject To Future Warming Water, Carolyn R. Wheeler

Graduate Doctoral Dissertations

Marine ectothermic fishes – fishes that do not regulate internal body temperature independent from the water temperature – are some of the most vulnerable organisms to rapid and alarming increases in environmental temperature occurring due to climate change mediated-ocean warming. For tropical fish species that live near their thermal maxima, this warming could be of grave consequence to individuals, populations, and whole marine ecosystems. Certain key life stages such as embryos and reproducing adults may be particularly vulnerable to warming given the excess energetic costs of these life stages, which may disallow compensation for extreme changes in temperature. One taxonomic …

Research Note: Association Of Single Nucleotide Polymorphism Of Akt3 With Egg Production Traits In White Muscovy Ducks (Cairina Moschata)., Semiu Folaniyi Bello, Haiping Xu, Kan Li, Lijin Guo, Siyu Zhang, Ridwan Olawale Ahmed, Endashaw Jebessa Bekele, Ming Zheng, Mingjian Xian, Bahareldin Ali Abdalla, Adeniyi Charles Adeola, Adeyinka Abiola Adetula, Raman Akinyanju Lawal, Weijian Zhu, Dexiang Zhang, Xiquan Zhang, Congliang Ji, Qinghua Nie

Faculty Research 2022

Prior studies on transcriptomes of hypothalamus and ovary revealed that AKT3 is one of the candidate genes that might affect egg production in White Muscovy ducks. The role of AKT3 in the uterus during reproductive processes cannot be overemphasized. However, functional role of this gene in the tissues and on egg production traits of Muscovy ducks remains unknown. To identify the relationship between AKT3 and egg production traits in ducks, relative expression profile was first examined prior to identifying the variants within AKT3 that may underscore egg production traits [age at first egg (AFE), number of eggs at 300 d …

An Evaluation Of Bovine Reproductive Function From The Microrna Expression In The Female To Performance Testing In The Male, Erin Amiss

All Theses

Reproductive function is crucial to the success of a beef or dairy herd. Both the female and male play significant roles in the outcome of bovine reproduction. The bull contributes to reproductive success in many ways, such as genetics. The initial role that the bull has in reproductive success is fertilization, whether natural or by artificial insemination. One method of assessing bull performance are central bull test stations, which are traditionally administered by a university, extension center, or are privately owned. Central bull tests assess the performance of bulls from various management systems, breeds, and ages. Some measurements taken during …

Purification And Characterization Of An Anti-Basigin Monoclonal Antibody: Application To The Study Of Human Uterine Cells And Tissues, Jeannine M. Barna

All NMU Master's Theses

Between 1990 and 2017, the global rate of female infertility increased by over 15% (Sun et al., 2019). An embryo's failure to implant into the uterus is the primary cause of early pregnancy loss. Several studies have identified molecules necessary for successful implantation, but their function during implantation remains poorly understood (S. Zhang et al., 2013). The cell surface glycoprotein basigin-2 is necessary for embryo implantation in the mouse and plays a role in several cellular functions, including cell communication and extracellular matrix remodeling (Chen et al., 2009; K. Li & Nowak, 2020). In vitro studies using human uterine cells …

Reproductive Traits And Change In Body Shape Of Neonates In The Oak Forest Skink, Plestiodon Lynxe, Manuel Feria-Ortiz, Uri Omar García-Vázquez, Carlos Joaquín Pavón-Vázquez, Adrián Nieto-Montes De Oca

Publications and Research

Reproductive traits are critically important for understanding how organisms adapt to their respective environments. In this study, we provide information on relative litter mass (RLM) and other litter and neonate related characters of nine female Plestiodon lynxe captured in the field. We also recorded seven body dimensions in 16 neonates and 15 two-month juveniles, and on the basis of these dimensions we compared the body shape of these two age classes to detect changes in the proportions of body parts. The average litter size (4.55) is larger than that found in other viviparous species of Plestiodon, but smaller than …

Climate Impacts On North American Quail, Shelby M. Perry, Erin Moser, Jeffrey G. Whitt, Kelly S. Reyna

National Quail Symposium Proceedings

North America’s quail population trends are often linked to regional climate. Extreme climate events such as severe drought, hard freezes, or excessive winter precipitation can reduce quail populations by as much as 84%. Above-average spring and summer temperatures coincident with drought can reduce the laying season for quail by ≤60 days. Exposure of quail eggs to high temperatures during preincubation can initiate and alter embryonic development. Here, we review the impacts of extreme climate events and a changing climate on the survival, reproduction, and population trends of 6 North American quail species: California quail (Callipepla californica), Gambel’s quail …

Applying Multistate Mark-Recapture Models With State Uncertainty To Estimate Survival And Reproduction Of Quail, Rebekah E. Ruzicka

National Quail Symposium Proceedings

Obtaining unbiased estimates of vital rates and understanding how vital rates change in response to environmental stimuli are a continual pursuit of ecologists. Multistate mark-recapture (MSMR) models provide a flexible framework for evaluating dependent vital rates in a comprehensive analysis. For example, a bird must remain alive during breeding season to initiate a nest (i.e., transition from a nonbreeding to a breeding state); thus, the probability that a bird initiates a nest is dependent on the probability that it is still alive. Traditional MSMR models allow only for the estimation of survival, detection, and state transition parameters and depend on …

Survival, Movement, And Habitat Use Of Translocated Northern Bobwhite In Texas, Ricardo Cagigal Perez, Nova J. Silvy, Brian L. Pierce, Therese A. Catanach

National Quail Symposium Proceedings

For decades there has been a noticeable decline in northern bobwhite (Colinus virginianus; hereafter, bobwhite) populations. Few studies have assessed the survival of translocated bobwhite. We evaluated the effectiveness of reintroduction of bobwhite into the Texas (USA) Parks and Wildlife Department’s Gus Engeling Wildlife Management Area (GEWMA), where they had been extirpated but now have suitable habitat. Before reintroduction, GEWMA was surveyed (spring call counts) to make sure no bobwhite were present. Forty-six bobwhite were trapped from March–April 2019 in South Texas, banded, bled, radio-tagged, transported to GEWMA, and released. In addition, 17 bobwhite were trapped banded, bled, …

Breeding Season Survival And Reproduction In A High-Density Bobwhite Population: A Case Study, William E. Palmer, Shane D. Wellendorf, D. Clay Sisson

National Quail Symposium Proceedings

The demographic behavior of northern bobwhite (Colinus virginianus; hereafter, bobwhite) populations at high densities could provide important insights into why bobwhite populations fluctuate. Therefore, we documented breeding season demographics of bobwhites to understand how prebreeding density influenced reproductive effort and postbreeding density on an intensively managed property in Leon County, Florida, USA, 2002–2006. We estimated prebreeding bobwhite density each April using multi-observer strip-transects and postbreeding densities each November using covey call grid surveys. We radio-tagged 217 bobwhites in March and located bobwhites at least 5 days/week, 15 April–30 September to determine vital rates. Prebreeding density ranged from 1.5–8.6 …

Role Of Nucleobindin-2/Nesfatin-1 In The Regulation Of Energetic Status And Maternal Mouthbrooding In The Cichlid Fish, Astatotilapia Burtoni, Saachi Chugh

LSU Master's Theses

Feeding and energetics are fundamental processes in all species that must also be balanced with reproductive investment. The peptide hormone nesfatin-1 is encoded by the nucleobindin-2(nucb2) gene and has recently emerged as a key player involved in both feeding and reproduction across vertebrates. How nesfatin-1 might help regulate trade-offs between feeding and reproduction, however, are relatively unexplored. The female cichlid fish Astatotilapia burtoniis an ideal system to examine the role of nesfatin-1 in regulating metabolic and reproductive functions because this species cycles between a gravid feeding state while preparing to mate, followed by a forced starvation …

Estimating Age In Mule Deer: Accuracy And Influence On Reproduction, Morgan S. Hinton

Theses and Dissertations

Accurate estimates of parameters and demographic rates of wildlife populations are crucial to management and conservation. One parameter of interest is age because it influences multiple demographic rates and behaviors including reproduction, dispersal, migration, and disease transmission. There are a number of methods used to estimate ages of wildlife. One of the most common methods used for mammals is evaluation of patterns in dental eruption and tooth wear. In the first chapter, we evaluate the accuracy associated with estimates of age from dental eruption and patterns of tooth wear for mule deer (Odocoileus hemionus). In the second chapter, we use …

Biological Flora Of Coastal Wetlands: Sporobolus Cynosuroides (L.) P.M. Peterson & Saarela, Richard Stalter, Robert I. Lonard

Biology Faculty Publications and Presentations

Sporobolus cynosuroides (L.) P.M. Peterson & Saarela¼Spartina cynosuroides (L.) Roth is a temperate zone rhizomatous grass that often is a dominant species in coastal brackish marshes on the Gulf coast and Atlantic coasts of the United States where salinity ranges from 0 to 10 psu. Sporobolus cynosuroides (L.) P.M. Peterson & Saarela ¼ Spartina cynosuroides (L.) Roth is usually absent where salinity values are .12 psu. Sporobolus cynosuroides occurs in coastal habitats characterized by infrequent tidal flooding and moderate nutrient levels. Also known as big cordgrass, it may account for net productivity in high marshes that rivals productivity of Sporobolus …

Reproductive Timing In Percina Aurora And Other Percina Species In The Pascagoula River Watershed, Malia Davidson

Honors Theses

The Southeastern United States is home to high levels of aquatic biodiversity and was recently named one of the Earth’s biodiversity hotspots. Within this region, growing human populations continue to place pressure on aquatic ecosystems and the resident species. Darters (Percidae: Percina) represent a large proportion of the freshwater fish diversity in the region. However, for some species the fundamentals of age, growth, reproductive timing and effort, and ecology remain poorly understood. That said, understanding the timing, duration, and effort allocated to reproduction is vital to successful management of any species. The pearl darter, Percina aurora, has been …

The Reproductive Biology Of Small Fishes And The Clutch Concept: Combining Macroscopic And Histological Approaches, David C. Heins, Nancy J. Brown-Peterson

Faculty Publications

Accurate estimates of reproductive parameters important in understanding life history evolution and conservation of small fishes are dependent upon careful assignment of ovarian phases. Proper assignment is based upon the stages of propagule (oocyte) development, oocyte maturation and the location of any clutch or portion thereof within the ovaries. Macroscopic inspection and assignment of ovarian developmental phases have often been used for small freshwater fishes. By contrast, histological methods for assignment of reproductive phases have been developed and are widely used for marine fishes, but they have rarely been used for small freshwater fishes. We review oocyte development, ovum maturation, …

Effects Of Administration Of A Growth Promoting Implant During The Suckling Phase Or At Weaning On Growth, Reproduction, And Ovarian Development In Replacement Heifers Grazing Native Range, Shelby L. Rosasco, Emily A. Melchior-Tiffany, Cierrah J. Kassetas, Shad H. Cox, Richard L. Dunlap, Jennifer A. Hernandez Gifford, Eric J. Scholljegerdes, Robert A. Cushman, Adam F. Summers

Roman L. Hruska U.S. Meat Animal Research Center: Reports

Management strategies utilized during pre-breeding development of replacement heifers can impact fertility and the ovarian reserve. Angus- Hereford crossbred heifers (n = 233) were utilized over a 3-yr period to determine the effects of administration of a growth promoting implant at either branding or weaning on growth, reproduction, and ovarian development. Heifer calves were randomly assigned to one of three treatments: 1) nonimplanted controls (CON; n = 79), 2) implanted at approximately 2 mo of age (average calf age = 58 d) with Synovex-C (BIMP, n = 82), or 3) implanted at approximately 7 mo of age (average calf …