Life Sciences Commons^™

Psd-Project Sequence Database, Matthew Kayala, Akhila Kambhampati, Stephanie Forschner, Bethany D. Jenkins

Bioinformatics Software Design Projects

1. Introduction

The sequencing of nucleic acids and the subsequent analysis of their encoded the genes and proteins has revolutionized biological sciences and allowed for the determination of evolutionary origins and biochemical potential of organisms. While the technology to determine molecular sequence data has become extremely powerful in terms of both automation and thoughput, sequence analysis technology has lagged behind, unable to efficiently keep up with the vast amount of sequence data being generated both in individual labs and by researchers worldwide. The foundation of robust sequence analysis is dependent on the building and maintaining of project sequence databases (PSD) …

Dolomedes Scriptus (Water Spider): Male Response To Females’ Putative Pheromones On Perches., Katherine Hunt

Undergraduate Theses and Capstone Projects

Sexual signals are commonly used among animals to enhance reproductive success. Chemical signals such as pheromones are commonly used to attract mates. Little is known, though, about how these signals are received or interpreted by the target. I studied the water spider, Dolomedes scriptus in the pisaurid family. Most Dolomedes species only make webs as a nursery for their young. However, females often create silk drag lines, potentially serving for species recognition. In some Dolomedes species, females who are previously mated are known to cannibalize most approaching males. Males might avoid cannibalism by avoiding mated females, particularly if they can …

Abstracts Of Papers, 84th Annual Meeting Of The Virginia Academy Of Science

Virginia Journal of Science

Full abstracts of papers for the 84th Annual Meeting of the Virginia Academy of Science, May 25-26, 2006, Virginia Polytechnic Institute and State University, Blacksburg, VA

Plasmid Probes For Screening Specific Genes In Large Fragment Dna Libraries, Leah Ferguson

Senior Honors Projects

Heliothis virescens, a member of the lepidopteran family, is of great importance to many people who rely on agriculture as a source of income because it is a pest of many important crops. Because H. virescens feeds on a broad array of hosts including cotton, tobacco, tomato, and soybean it has earned its place as a major nuisance in the United States. Heliothis subflexa is a close taxonomic relative of H. virescens, but the two species differ in that H. subflexa is a specialized herbivore that only feeds on plants in the genus Physalis. Studies have been performed to determine …

Regulation Of Cytoplasmic Dynein Atpase By Lis1, Mariano T. Mesngon, Cataldo Tarricone, Sachin Hebbar, Aimee Guillotte, E. William Schmitt, Lorene Lanier, Andrea Musacchio, Stephen J. King, Deanna S. Smith

Faculty Publications

Mutations in Lis1 cause classical lissencephaly, a developmental brain abnormality characterized by defects in neuronal positioning.Over the last decade, a clear link has been forged between Lis1 and the microtubule motor cytoplasmic dynein. Substantial evidenceindicates that Lis1 functions in a highly conserved pathway with dynein to regulate neuronal migration and other motile events. Yeasttwo-hybrid studies predict that Lis1 binds directly to dynein heavy chains (Sasaki et al., 2000; Tai et al., 2002), but the mechanistic significance of this interaction is not well understood. We now report that recombinant Lis1 binds to native brain dynein and significantly increases the microtubule-stimulated enzymatic …

Use Of Lolium Multiflorum For Remediation Of Phosphorus From Poultry-Litter-Contaminated Media, Daniel L Starnes

Mahurin Honors College Capstone Experience/Thesis Projects

No abstract provided.

Contribution To The Taxonomical And Biological Characteristics Of Sciurus Anomalus In Turkey (Mammalia: Rodentia), İrfan Albayrak, Ati̇lla Arslan

Turkish Journal of Zoology

This study is based on 62 Sciurus anomalus specimens obtained from Konya, Karaman, Kütahya and Çorum provinces between 1997 and 2002. Some ecological and biological features of the species, including habitat, nest-sites, fur colour, baculum and phallus were investigated. In this species, the chromosome number (2n) is 40. For the taxonomical evaluation of Sciurus anomalus, morphometric data of the specimens were used. The specimens were discussed at the subspecies level, comparing to the relevant literature, and it was concluded that our specimens represent the nominate form.

Establishment Of Polarity During Organization Of The Acentrosomal Plant Cortical Microtubule Array, Ram Dixit, Eric Chang, Richard Cyr

Biology Faculty Research

The plant cortical microtubule array is a unique acentrosomal array that is essential for plant morphogenesis. To understand how this array is organized, we exploited the microtubule (+)-end tracking activity of two Arabidopsis EB1 proteins in combination with FRAP (fluorescence recovery after photobleaching) experiments of GFP-tubulin to examine the relationship between cortical microtubule array organization and polarity. Significantly, our observations show that the majority of cortical microtubules in ordered arrays, within a particular cell, face the same direction in both Arabidopsis plants and cultured tobacco cells. We determined that this polar microtubule coalignment is at least partially due to a …

Identifying Exclusion Genes Of Bacteriophage Hk239, Ali Wright

Mahurin Honors College Capstone Experience/Thesis Projects

Temperate bacteriophage can adopt either a lytic or a lysogenic lifestyle. In the lytic lifestyle, the bacteriophage infects a cell, uses the host's cellular machinery to replicate, and lyses the cell to release the phage progeny. In the lysogenic lifestyle the bacteriophage genome is integrated into the bacterial genome to generate a prophage. Most of the genes of the prophage are repressed. However, those genes that are expressed have a wide variety of functions including exclusion, the prevention of phage infection. Lysogens of bacteriophage HK239 have the ability to exclude a wide variety of phage, including (lambda), T4rII, Plvir, P2, …

Photochemical Transformations Of Riverine Dissolved Organic Matter: Effects On Estuarine Bacterial Metabolism And Nutrient Demand, Erik M. Smith, Ronald Benner

Faculty Publications

By experimentally simulating the exposure of terrigenous dissolved organic matter (DOM) to sunlight during the mixing of river water and seawater in an estuary (St. Helena Sound, South Carolina), this study examined how photochemical alterations may affect both the magnitude and fate of DOM utilization by estuarine bacteria. In a series of experiments performed with water samples collected from 3 blackwater rivers draining into an estuary of the South Atlantic Bight (USA), exposure to light resulted in significant decreases in dissolved organic carbon (DOC) concentration and DOM absorption at 350 nm relative to dark controls. When these water samples were …

Phosphotyrosine Phosphatases Of The Att-20 Murine Corticotroph Cell Line, Kari A. Belin

Seton Hall University Dissertations and Theses (ETDs)

Somatostatin (somatotropin release inhibitory factor; SRIF) is a peptide-signaling molecule that activates a family of heterotrimeric guanine nucleotide binding protein (G­ protein) coupled receptors (sst.-sst-). SRIF receptors control essential intracellular signaling events and, by reducing cyclic nucleotide levels, ion concentrations and protein phosphorylation, ultimately controlling cell proliferation and secretion. In the current study, we investigated the intracellular phosphatase activity present in the AtT-20 cell, as well as whether these enzymes were under direct SRIF receptor control. AtT-20 cells retain many of the properties of anterior pituitary corticotrophs, yet are an established cell line that expresses at least two SRIF receptor …

Law And Behavioral Biology, Owen D. Jones, Timothy H. Goldsmith

Vanderbilt Law School Faculty Publications

Society uses law to encourage people to behave differently than they would behave in the absence of law. This fundamental purpose makes law highly dependent on sound understandings of the multiple causes of human behavior. The better those understandings, the better law can achieve social goals with legal tools. In this Article, Professors Jones and Goldsmith argue that many long held understandings about where behavior comes from are rapidly obsolescing as a consequence of developments in the various fields constituting behavioral biology. By helping to refine law's understandings of behavior's causes, they argue, behavioral biology can help to improve law's …

Size And Organic Content Of Eggs Of Marine Annelids, And The Underestimation Of Egg Energy Content By Dichromate Oxidation, William Jaeckle, Bruno Pernet

Scholarship

Dichromate oxidation is a simple technique that is often used to estimate the energy content of eggs in studies of marine invertebrate life histories (1). We used this method to measure the energy contents of the eggs of 12 species of marine annelids. In combination with measures of egg ash-free dry weight (AFDW), these data yielded estimates of AFDW-specific energy density that were mostly lower than the average weight-specific energy density of carbohydrates. This seemed unlikely to be correct, as invertebrate eggs typically contain little carbohydrate and instead are composed primarily of energy-dense protein and lipid (1, 2). After validating …

A Patch Hath Smaller Patches: Delineating Ecological Neighborhoods For Parasites, Derek Zelmer, John R. Seed

Faculty Publications

Use of the host individual as a boundary for parasite populations and communities provides an unambiguous spatial unit that is useful for pattern description, but this framework precludes consideration of the host landscape and within-host population dynamics. Recognizing host individuals as spatially and temporally complex landscapes requires modified concepts of parasite populations and communities. An outline of the currently accepted hierarchies of parasite populations and communities is provided on the basis of ecological neighborhoods that are delineated by discrete habitat patches or functional dynamics (or both), as opposed to host individuals. This parasite-based framework accommodates consideration of both within- and …

Individual Cell Growth Rates Of Marine Bacteria, Measured By Bromodeoxyuridine Incorporation, Koji Hamasaki, Richard A. Long, Farooq Azam

Faculty Publications

We tested the application of 5-bromo-2’-deoxyuridine (BrdU), a thymidine analogue that becomes incorporated into DNA during growth, to measure growth rates of individual marine bacteria cells. Immunocytochemical detection of BrdU incorporation into bacterial DNA has the potential for single-cell-based growth measurement. Optimized procedure for immunocytochemistry was applicable to 14 marine heterotrophic bacterial isolates belonging to g-proteobacteria, α-proteobacteria, Cytophaga-Flavobacterium-Bacteroides (CFB) group and Gram-positive bacteria. The relationship between cell-specific fluorescence intensity and specific growth rate was linearly correlated among CFB group isolates, which indicated a potential of the method for quantitative measurement. Analysis of the detection limit indicated …

Particle Removal Rates By The Mud Shrimp Upogebia Pugettensis, Its Burrow, And A Commensal Clam: Effects On Estuarine Phytoplankton Abundance, Blaine D. Griffen, Theodore H. Dewitt, Chris Langdon

Faculty Publications

The burrowing shrimp Upogebia pugettensis is an abundant intertidal invertebrate of Pacific Northwest, USA bays and estuaries where it lives commensally with the bivalve Cryptomya californica. Suspension-feeding activities by the shrimp and by its commensal clam, as well as particle settlement within the burrow, represent 3 different components that could remove phytoplankton from water drawn into shrimp burrows. These 3 components together comprise what we call the ‘U. pugettensis shrimp-burrow complex’. In laboratory experiments, we measured particle removal by each of these components. Our results indicated that U. pugettensis itself is responsible for filtering the majority of phytoplankton removed …

Variability In Broods Of The Seastar Leptasterias Aequalis, William B. Jaeckle, Brian L. Bingham, Kehualani Giles

Scholarship

Enormous variation exists in the reproductive output of marine invertebrates (e.g., in the numbers of em¬bryos produced, the volumes of embryos, and the energy that they contain). It is not clear why there is such great variability or what the population-level consequences are. We sampled a population of the brooding seastar Leptasterias aequalis (Stimpson, 1862) to collect basic information on brood sizes, embryo volume, and embryo energy content with a goal to better understand the reproductive ecology of this species. We collected brooding females in February and again in April. We measured the size of their broods and sampled the …

The Cortical Microtubule Array: From Dynamics To Organization, Ram Dixit, Richard Cyr

Biology Faculty Research

Cortical microtubules (CMTs) are essential for normal plant morphogenesis because they affect the axes of cell elongation and predict the placement of cell division planes. The function of the CMTs is intimately linked to their organizational state, which is subject to spatial and temporal modifications by developmental and environmental cues. CMT assembly dynamics govern when, where, and how microtubules appear in a cell's cortex, and the regulation of these properties affects their organization. However, the principles that link microtubule assembly dynamics to cortical array organization and reconfiguration are not well understood. This essay focuses on recent advances in the understanding …

Encounters Between Dynamic Cortical Microtubules Promote Ordering Of The Cortical Array Through Angle-Dependent Modifications Of Microtubule Behavior, Ram Dixit, Richard Cyr

Biology Faculty Research

Ordered cortical microtubule arrays are essential for normal plant morphogenesis, but how these arrays form is unclear. The dynamics of individual cortical microtubules are stochastic and cannot fully account for the observed order; however, using tobacco (Nicotiana tabacum) cells expressing either the MBD-DsRed (microtubule binding domain of the mammalian MAP4 fused to the Discosoma sp red fluorescent protein) or YFP-TUA6 (yellow fluorescent protein fused to the Arabidopsis alpha-tubulin 6 isoform) microtubule markers, we identified intermicrotubule interactions that modify their stochastic behaviors. The intermicrotubule interactions occur when the growing plus-ends of cortical microtubules encounter previously existing cortical microtubules. Importantly, the outcome …

Scale Model Of A Soil Aggregate And Associated Organisms: A Teaching Tool For Soil Ecology, Mary Anne Bruns, Loren B. Byrne

Arts & Sciences Faculty Publications

Soil is a complex habitat for diverse biota. A significant challenge in teaching soil ecology is our inability to observe organisms as they live and interact in the soil. The objective of this article is to describe an interactive class project to help students visualize the sizes of different groups of soil organisms and to relate these to soil structural components. This project was carried out by students in an upper-level undergraduate soil ecology class. It involved the design and construction of a 4000× scale model of a soil aggregate and its associated organisms. The body of the model was …

From Biology To Consciousness To Morality, Ursula Goodenough, Terrence W. Deacon

Biology Faculty Research

Social animals are provisioned with prosocial orientations that operate to transcend self-interest. Morality, as used here, describes human versions of such orientations. We explore the evolutionary antecedents of morality in the context of emergentism, giving considerable attention to the biological traits that undergird awareness and our emergent human forms of mind. We suggest that our moral frames of mind emerge from our primate prosocial capacities, transfigured and valenced by our symbolic languages, cultures, and religions.

Portions of this article were given by Deacon in a paper at the forty-ninth annual conference of IRAS, “Is Nature Enough? The Thirst for …

P-Limitation Of Respiration In The Sargasso Sea And Uncoupling Of Bacteria From P-Regeneration In Size-Fractionation Experiments, Ingrid Obernosterer, Nobu Kawasaki, Ronald Benner

Faculty Publications

Community respiration rates were measured in unfiltered seawater collected in the upper 75 m of the water column along a transect in the Sargasso Sea and at the Bermuda Atlantic Time-series Study (BATS) station (31° 50’ 00 N; 64° 10’ 00 W) during a cruise in June and July 2001. Community respiration rates in the upper 75 m of the water column averaged 1.1 ± 0.4 μM O₂ d^-1 and exhibited significant spatial and temporal variability. Concurrent determination of the heterotrophic and autotrophic community revealed no relationship between community respiration and the abundance of any of the major …

Microalgal-Meiofaunal Trophic Relationships In Muddy Intertidal Estuarine Sediments, James L. Pinckney, Kevin R. Carman, S. Elizabeth Lumsden, Sabrina N. Hymel

Faculty Publications

The determination of the primary factors controlling carbon (C) transfer rates in situ are a basic requirement for developing a mechanistic view of the processes that regulate benthic microbial community structure and function. In many estuarine ecosystems, the base of the food web is dominated by microalgal production; however, information on the quantitative relationships and process rates of meiofaunal grazers and benthic microalgal (BMA) producers is limited to a few habitats and short temporal scales. The primary objective of this study was to examine the trophic interactions between meiofaunal grazers and BMA producers to determine if grazing is a primary …

A Watershed Analysis Of Threemile Pond: Implications For Water Quality And Land Use Management, Colby Environmental Assessment Team, Colby College, Problems In Environmental Science Course (Biology 493), Colby College

Colby College Watershed Study: Threemile Pond (2003)

The 2003 Colby Environmental Assessment Team chose to study the Threemile Pond watershed. The Threemile Pond watershed is located in Vassalboro, China., Windsor, and Augusta, Maine. Threemile Pond is a popular site for recreation and is home to a wide range of flora and fauna. Like all other lakes in Maine, it is a young lake. However, intensive human activity in the watershed contributes a substantial amount of nutrients and the lake has algal blooms annually in the summer months. The purpose of this study was to assess the impact of land use and development on the water quality of …

Ec03-176 Noxious Weeds Of Nebraska: Musk Thistle, Fred Roeth, Steven R. Melvin, Irvin L. Schleufer

University of Nebraska-Lincoln Extension: Historical Materials

Musk Thistle is an introduced invasive broadleaf weed native to Europe, North Africa, and Asia. In these areas it is a minor weed because natural enemies keep its population low. When the plant was introduced into North America, its natural enemies were left behind. Without these natural checks, the thistle is able to thrive and compete with native vegetation. This Extension Circular discusses the history, biology, identification, distribution and control of the musk thistle.

Ec03-177 Noxious Weeds Of Nebraska: Purple Loosestrife, Stevan Z. Knezevic

University of Nebraska-Lincoln Extension: Historical Materials

Purple loosestrife is an introduced invasive weed that is overrunning thousands of acres of wetlands and waterways in the Midwest. Once purple loosestrife invades a wetland, natural habitat is lost and the productivity of native plant and animal communities is severely reduced. This Extension Circular discusses the biology, identification, distribution and control of purple loosestrife.

Genes And Justice: The Future Of Cloning In The United States - An Analysis Of Ethical, Scientific, & Legal Policy, Marlo Diane Curiel

Whittier Scholars Program

This project is a report that surveys the “Biotech Age” expansion and the resulting ethical dilemmas in regards to human cloning while providing an analysis of ethical, scientific, and legal policy. This project is split into sections which discuss the Biotech Age including the Biotech trends, recent legal and scientific breakthroughs, and insight into controversy. This project revolves around the science of cloning human beings, ethical dilemma and legal background. In conclusion a cloning bill is proposed by the scholar.

John Russell Bozeman Papers, Zach S. Henderson Library Special Collections

Finding Aids

This collection consists of research materials belonging to Georgia Southern College instructor, John Russell Bozeman. Materials span 1948-1994 and include research files on beach erosion, the ecology of the Georgia barrier islands, and general information on Southeastern ecology and topography.

Find this collection in the University Libraries' catalog.

Improving The Mass Rearing Possibilities Of Serangium Montazerii Fürsch (Coleoptera: Coccinellidae) On Different Host Plants Of Bemisia Tabaci (Genn.) (Homoptera: Aleyrodidae), Gonca Vatansever, M. Rifat Ulusoy, Lerzan Bakircioğlu Erkiliç

Turkish Journal of Agriculture and Forestry

The objectives of this study were to identify suitable plant species for mass rearing and to determine some biological characteristics of the predator Serangium montazerii Fürsch (Coleoptera: Coccinellidae) used in the biological control of Dialeurodes citri (Ashmead) (Homoptera: Aleyrodidae). The development time, mortality rate, preoviposition, oviposition and postoviposition periods and fecundity of S. montazerii were studied on various host plants using Bemisia tabaci (Genn.) (Homoptera: Aleyrodidae). Two cotton (Çukurova-1518 and Deltapine) and two eggplant (Pala and Çukurova topağı) cultivars were used at 25 ºC and 65 ± 5% RH in growth chambers. Additionally, life tables were constructed from the data …

Trends. Terrorist Brains, Ibpp Editor

International Bulletin of Political Psychology

This Trends article discusses the construct of biological reductionism in the context of the case of Red Army Faction member Ulrike Meinhof. For the author, overly emphasizing the biological aspects of Ms. Meinhof’s case might obscure legitimate grievances, and ultimately, lead to more cases of violence associated with terrorism.