Life Sciences Commons^™

Healing Of Prosthetic Arterial Grafts, G. Pasquinelli, A. Freyrie, P. Preda, T. Curti, M. D'Addato, R. Laschi

Scanning Microscopy

Numerous synthetic biomaterials have been developed as vascular substitutes. In vitro, ex vivo and in vivo studies have demonstrated that in animals, selected materials, i.e., Dacron and ePTFE (expanded polytetrafluoroethylene) grafts, are successfully incorporated in both the large and the small caliber host arteries through a process which is generally referred to as graft healing. Morphologically, this process consists of a series of complex events including fibrin deposition and degradation, monocyte-macrophage recruitment and flow-oriented cell-layer generation, this last event being the complete endothelialization of the arterial substitute.

In contrast to experimental animals, the flow surface of synthetic vascular grafts remains …

Ec90-2502 Perspectives On Nitrates, Gary W. Hergert, Richard A. Wiese, Delynn Hay, William A. Lee, Ann Ziebarth, Richard B. Davis, Constance Kies, Carolyn Bednar, Norman Schneider, Alex Hogg, Robert A. Britton, J. David Aiken

University of Nebraska-Lincoln Extension: Historical Materials

The potential adverse consequences of nitrate on both human and animal health has long been recognized. This extension circular is comprised of six papers that include:

• Nitrogen in our Environment
• Alternatives When Excessive Nitrate is Present in Drinking Water
• Nitrates, Nutrites and Methemoglobinemia
• Nitrates, Nutrities, N-Nitroso Compounds and Nutrition
• Excessive Nitrate/Nitrite Exposure: Nitrate Poisoning and Related Animal Health Effects
• Nitrates and Ground Water Quality Protection Policies

Embryonic Chicken Fibroblast Collagen Binding Proteins: Distribution, Role In Substratum Adhesion, And Relationship To Integrins, Roy C. Ogle, A. Jeannette Potts, Marchall Yacoe, Charles D. Little

School of Medical Diagnostics & Translational Sciences Publications

Collagen binding proteins (CBP) are hydrophobic, cell surface polypeptides, isolated by collagen affinity chromatography. Antibodies to CBPs inhibit the attachment of embryonic chicken heart fibroblasts to native type I collagen fibrils in a dose-dependent manner. The CBP antibodies also induce rounding and detachment of cells adherent to a planar substratum. This process of antibody-mediated substratum detachment resulted in a clustering of CBP and cell-associated extracellular matrix at the cell surface, and the rearrangement of filamentous actin. Other functional studies showed that cells grown within a three-dimensional gel of type I collagen cannot be immunostained at the cell surface with CBP …

G89-919 Quality Laboratory Samples Necessary For Accurate Disease Diagnosis, Duane Rice, Douglas G. Rogers

University of Nebraska-Lincoln Extension: Historical Materials

This NebGuide discusses the importance of submitting proper specimens to the veterinary diagnostic laboratory as an aid in diagnosing infectious diseases, feed imbalance, or animal poisoning.

To comprehend the reasons for failures in disease diagnosis at veterinary diagnostic laboratories, it is important to understand what disease is, what causes disease, how the animal resists disease (becomes immune), and what happens to the animal if disease develops and the animal does not become immune.

According to Stedmans Dictionary, disease is an interruption, cessation or disorder of body functions, systems or organs. Some diseases may be obvious to the untrained eye, …

A Light And Electron Microscopic Study Of The Rat Olfactory Tubercle: Normal Morphology And Acetylcholinesterase Localization, James Curtis Woodley

Biological Sciences Theses & Dissertations

A cytoarchitectural analysis of the rat olfactory tubercle using Nissl-stained coronal sections revealed that the dense cell layer (DCL) consisted of medium sized striatal cells in the cortical regions and small sized "granule" cells in the cap regions. Also delineated from this experiment was a rim of neuropil, nearly devoid of neurons as well as neuronal processes, outlining the islands of Calleja. Acetylcholinesterase (AChE) localization utilizing light microscopy revealed that the olfactory tubercles (OT) contained AChE-positive fibers that were orientated dorsoventrally in the molecular and multiform layers. The DCL consisted of only fibers en passant and putative terminals. Diisopropylfluorophosphate (DFP) …

The Effect Of Iron And Lead Inhibition, Beth Brownell

Whittier Scholars Program

This project is an experiment to test the hypothesis that the amount of iron present in the body affects the biosynthetic pathway of heme in experimental animals. This study was done by monitoring the protoporphyrin IX blood levels in four mice groups: no lead in drinking water with normal iron pellet diet, no lead in drinking water with no iron pellet diet, 1mM lead in drinking water with normal iron pellet diet, and 1mM lead in drinking water with no iron pellet diet. Fluorescence spectra of diluted whole blood from lead-exposed mice with different iron levels were found to be …

Parentage Analysis With Genetic Markers In Natural Populations I The Expected Proportion Of Offspring With Unambiguous Paternity, R Chakraborty, T R Meagher, P E Smouse

Faculty, Staff and Student Publications

Recent studies indicate that polymorphic genetic markers are potentially helpful in resolving genealogical relationships among individuals in a natural population. Genetic data provide opportunities for paternity exclusion when genotypic incompatibilities are observed among individuals, and the present investigation examines the resolving power of genetic markers in unambiguous positive determination of paternity. Under the assumption that the mother for each offspring in a population is unambiguously known, an analytical expression for the fraction of males excluded from paternity is derived for the case where males and females may be derived from two different gene pools. This theoretical formulation can also be …

Response Of Breeding Birds To Commercial Clearcutting Of Aspen In Southwestern Colorado [Usa], V.E. Scott, G.L. Crouch

Aspen Bibliography

Breeding birds on an aspen forest in southwestern Colorado increased in species diversity after 25% of the timber sale area forest was clearcut in patches of 3 to 20 acres. Bird population density on the forest with clearcuts was not significantly different from that on an uncut forest. Of the 20 species evaluated, six were more and one was less abundant than on the uncut forest.

In Vitro Reconstitution Of Exocytosis From Sea Urchin Egg Plasma Membrane And Isolated Cortical Vesicles, Joseph H. Crabb, Paul A. Modern, Robert C. Jackson

Dartmouth Scholarship

We have succeeded in reconstituting an exocytotically active egg cortex fraction by recombining purified cortical vesicles (CVs) with egg plasma membrane (PM). CVs were dislodged from a suspension of egg cortex by gentle homogenization in a dissociative buffer with a pH of 9.1, and purified by two rounds of differential centrifugation. Egg PM was prepared by shearing the cortical vesicles from a cortical lawn preparation with a jet of isotonic buffer. PM lawns produced by this procedure consist of an array of CV-free PM fragments attached via their extracellular surface to a polylysine coated glass slide. When a neutralized suspension …

The Homeo Domain Of A Murine Protein Binds 5' To Its Own Homeo Box., Abraham Fainsod, Leonard D. Bogarad, Tarmo Ruusala, Martin Lubin

Dartmouth Scholarship

Nuclear protein extracts from day 12.5 mouse embryos were used to study protein binding to DNA sequences 5' of the Hox 1.5 homeo box. Embryos of this developmental stage are known to express this gene. DNA binding protein blotting and retardation gel techniques show that murine embryonic nuclear proteins specifically bind a 753-base pair (bp) DNA fragment from the region upstream of the Hox 1.5 homeo box. A fusion protein containing the Hox 1.5 homeo domain constructed in lambda gt11 also binds the same 753-bp DNA fragment. Specific binding of the fusion protein to the upstream DNA fragment shows that …

Study Of A Small Mammal Population In Bowling Green, Kentucky, Richard Cambron

Masters Theses & Specialist Projects

The small mammal population of a restricted study area was live-trapped on the University farm at Western Kentucky University from November 1984 through November 1985. A total of 1800 trap-nights yielded 81 different individuals representing five species. Members of the genera Peromyscus and Microtus were the dominant individuals representing over 90% of the total. Also caught were Mus musculus and Blarina brevicauda. The members of the two major genera were examined for population size and dynamics. In addition, a statistical analysis was carried out on the randomness of the location of the home ranges. This analysis gave evidence that …

Atp-Dependent Formation And Motility Of Aster-Like Structures With Isolated Calf Brain Microtubule Proteins., Richard C. Weisenberg, Robert D. Allen, Shinya Inoue

Dartmouth Scholarship

Microtubule proteins isolated from calf brain will undergo gelation-contraction in the presence of ATP. We have now examined this process by video-enhanced contrast microscopy. After ATP addition to steady-state microtubules, slow (1-5 micron/min), linear movements of particles and microtubules toward aggregation centers occur. The resulting structures resemble mitotic spindle asters. During the time when gel contraction occurs, asters move (at 1-5 micron/min) toward other nearby asters. This is accompanied by the apparent shortening of the microtubules running between the asters. This is the first example of isolated microtubules undergoing a process that has similarities to half-spindle shortening during anaphase A. …

Absence Of A Structural Basis For Intracellular Recognition And Differential Localization Of Nuclear And Plasma Membrane-Associated Forms Of Simian Virus 40 Large Tumor Antigen., Donald L. Jarvis, Charles N. Cole, Janet S. Butel

Dartmouth Scholarship

The simian virus 40 large tumor antigen (T-ag) is found in both the nuclei (nT-ag) and plasma membranes (mT-ag) of simian virus 40-infected or -transformed cells. It is not known how newly synthesized T-ag molecules are recognized, sorted, and transported to their ultimate subcellular destinations. One possibility is that these events depend upon structural differences between nT-ag and mT-ag. To test this possibility, we compared the structures of nT-ag and mT-ag from simian virus 40-infected cells. No differences between the two forms of T-ag were detected by migration in polyacrylamide gels, by Staphylococcus aureus V8 partial proteolytic mapping of methionine- …

G86-790 Fumigating Farm-Stored Grain With Aluminum Phosphide (Revised May 1998), Clyde Ogg, David L. Keith

University of Nebraska-Lincoln Extension: Historical Materials

This NebGuide provides step-by-step instructions for fumigating stored grain on the farm with aluminum phosphide.

Fumigants act on all insect life stages. They control pests by diffusing through the air spaces between grain kernels as well as into the kernel itself. Fumigants are able to penetrate into places that are inaccessible to insecticide sprays or dusts.

Regardless of formulation, all fumigants are poisonous and toxic to humans and other warm-blooded animals as well as to insects and other pests. Because fumigant chemicals are highly toxic and hazardous to use, they are Restricted Use pesticides. They can only be used by …

Vertical Migration Of Onychiurus Subtenuis (Collembola) In Relation To Rainfall And Microbial Activity, M. Hassall, S. Visser, D. Parkinson

Aspen Bibliography

No abstract provided.

Two Separable Functional Domains Of Simian Virus 40 Large T Antigen: Carboxyl-Terminal Region Of Simian Virus 40 Large T Antigen Is Required For Efficient Capsid Protein Synthesis., Joanne Tornow, Maryellen Polvino-Bodnar, George Santangelo, Charles N. Cole

Dartmouth Scholarship

The carboxyl-terminal portion of simian virus 40 large T antigen is essential for productive infection of CV-1 and CV-1p green monkey kidney cells. Mutant dlA2459, lacking 14 base pairs at 0.193 map units, was positive for viral DNA replication, but unable to form plaques in CV-1p cells (J. Tornow and C.N. Cole, J. Virol. 47:487-494, 1983). In this report, the defect of dlA2459 is further defined. Simian virus 40 late mRNAs were transcribed, polyadenylated, spliced, and transported in dlA2459-infected cells, but the level of capsid proteins produced in infected CV-1 green monkey kidney cells was extremely low. dlA2459 large T …

Ruffed Grouse Habitat Relationships In Aspen And Oak Forests Of Central Wisconsin Usa, J.F. Kubisiak

Aspen Bibliography

Ruffed grouse habitat requirements and management opportunities have been defined by Bump et al. (1947), Grange (1948), Dorney (1959), Gullion at al. (1962), Moulton (1968), and Gullion (1972), among others. It is generally agreed that interspersion of cover types and age classes is one of the keys to better grouse populations.

Isolation Of A Collagenase Cdna Clone And Measurement Of Changing Collagenase Mrna Levels During Induction In Rabbit Synovial Fibroblasts., Robert H. Gross, Lynn A. Sheldon, Colin F. Fletcher, Constance E. Brinckerhoff

Dartmouth Scholarship

To facilitate our studies on the mechanisms controlling collagenase production at a molecular level in rabbit synovial fibroblasts, we have constructed a cDNA library using mRNAs isolated from cells induced with crystals of monosodium urate monohydrate. We have screened this library with cDNA probes made from induced and control mRNA populations. From among 30 clones that hybridized preferentially to the induced-cell probe, 4 contained collagenase sequences. The largest, a clone of 650 base pairs, was identified by its ability to hybrid select a mRNA that could be translated in a cell-free system into a product that was precipitable with monospecific …

Evidence For Hydrated Spermidine-Calf Thymus Dna Toruses Organized By Circumferential Dna Wrapping, Kenneth A. Marx, George C. Ruben

Dartmouth Scholarship

In spermidine-condensed calf thymus DNA preparations, torus–shaped condensates were shown by transmission electron microscopy to exist under the hydrated conditions of the freeze fracture experiment. Using extremely low Pt metal deposition levels (9 A Pt/C) high–contrast replicas of the spermidine–DNA toruses were obtained that showed circumferential wrapping of single DNA double helix–size surface fibres. Stereoscopic analysis of high magnification stereomicrographs established some details of the three-dimensional organization of two DNA double helix sections winding circumferentially on the inner surface of one such torus. These measurements demonstrate the usefulness of stereoscopic analysis of these high macromolecular organization magnification. Measurements on a …

An Indirect Method To Estimate The Aerial Biomass Of Small Single Stemmed Woody Plants, R.D. Fitzgerald

Aspen Bibliography

No abstract provided.

Monoclonal Antibodies To Novel Myeloid Antigens Reveal Human Neutrophil Heterogeneity., Edward D. Ball, Robert F. Graziano, Li Shen, Michael W. Fanger

Dartmouth Scholarship

Three cytotoxic murine monoclonal antibodies that recognize myeloid-specific antigens have been produced by immunization with normal human neutrophils or myeloblasts from a patient with acute myelomonocytic leukemia. Two of these, PMN 6 and PMN 29, are specific for neutrophils; the third monoclonal antibody, AML-2-23, is reactive with the majority of normal monocytes as well as a subpopulation of mature neutrophils. Although neutrophils from all individuals tested expressed these antigens, cytofluorographic analysis revealed that the percentage of cells bearing the PMN 6 and AML-2-23 antigens varied among individuals. Significant additional heterogeneity in the density of each antigen among antigen-bearing cells was …

The Cytoskeletal System Of Nucleated Erythrocytes. I. Composition And Function Of Major Elements, William D. Cohen, Diana Bartelt, Richard Jaeger, George M. Langford, Iris Nemhauser

Biology - All Scholarship

We have studied the dogfish erythrocyte cytoskeletal system, which consists of a marginal band of microtubules (MB) and trans-marginal band material (TBM). The TBM appeared in whole mounts as a rough irregular network and in thin sections as a surface-delimiting layer completely enclosing nucleus and MB. In cells incubated at 0 degrees C for 30 min or more, the MB disappeared but the TBM remained. MB reassembly occurred with rewarming, and was inhibited by colchicine. Flattened elliptical erythrocyte morphology was retained even when MBs were absent. Total solubilization of MB and TBM at low pH, or dissolution of whole anucleate …

Genetically Controlled Variation Of “Acid” Beta-Galactosidase Detected In Rattus Norvegicus By Isoelectric Focusing, T C Douglas, K A Kimmel, P E Dawson

Faculty, Staff and Student Publications

Two genetically variant forms of rat "acid" beta-galactosidase were found to differ in isoelectric point and pH dependence, but not in thermostability or sensitivity to inhibition by p-mercuribenzoate (PMB). The results of two backcrosses and an intercross indicated that the isoelectric focusing phenotypes are controlled by two codominant alleles at a single autosomal locus, for which we propose the name Glb-1. No significant linkage between Glb-1 and albino (LG I), brown (LG II), or hooded (LG VI) was observed. Strain-specific differences in total levels of kidney beta-galactosidase were detected, but it is not yet known whether the variation is controlled …

Synthesis Of Low Molecular Weight Heat Shock Peptides Stimulated By Ecdysterone In A Cultured Drosophila Cell Line., Robert C. Ireland, Edward M. Berger

Dartmouth Scholarship

Treatment of Schneider's line 3 Drosophila cells with the steroid hormone ecdysterone rapidly stimulated the synthesis and accumulation of the polypeptide previously designated p7 [Berger, E. M., Ireland, R. C. & Wyss, C. (1980) Somatic Cell Genet. 6, 119-129]. In this report, p7 is identified as the 23,000-dalton heat shock polypeptide (hsp23). In addition to hsp23, the synthesis of the low molecular weight heat shock polypeptides hsp22, hsp26, and hsp27 was also stimulated by ecdysterone, although to different extents. Hybridization of a nick-translated genomic clone containing the hsp23 gene to a total RNA blot showed that ecdysterone stimulation of hsp23 …

The Problem Of Pain: What Do Animals Really Feel?, Dana H. Murphy

Sentience Collection

Much of the contention and confusion that seem inevitably to arise whenever the subject of pain in animals comes up appear to stem principally from problems with the word "pain" itself. When used to describe responses in humans, "pain" can mean any subset of an incredibly broad spectrum of sensations and emotions, ranging from the instantaneous, galvanizing effect of a dentist drill hitting the nerve in a molar, to more airy notions such as the "pain" of rejection or "painfully" embarrassing situations. Humans even use concepts as abstruse as the German term, weltschmerz, or "world pain," which denotes a vaguely …

Detergent Extraction Of A Presumptive Gating Component From The Voltage-Dependent Sodium Channel, W J. Culp, D T. Mckenzie

Dartmouth Scholarship

A physiologically characterized radiolabeled neurotoxin complex obtained from venom of the scorpion Leiurus quinquestriatus has been used to identify detergent-solubilized presumptive sodium channel components in sucrose gradients. This toxin-binding component is found in extracts prepared from three sources of excitable membrane but appears to be absent from similar extracts prepared from nonexcitable membrane or from Torpedo californica membrane. Procedures that destroy the physiological activity of the Leiurus neurotoxin lead to a corresponding loss of toxin binding to the putative sodium channel component. The major component recognized by the Leiurus toxin sediments at 6.5 S. Scatchard analysis of quantitative binding experiments …

The Isolation Of Ecdysterone Inducible Genes By Hybridization Subtraction Chromatography, Michael P. Vitek, Susan G. Kreissman, Robert H. Gross

Dartmouth Scholarship

We have developed a procedure for selectively enriching a mRNA population for inducible sequences. Other than the induced mRNA species, the population of mRNA in control cells is approximately the same as the mRNA population in induced cells. Cytoplasmic mRNA from control cells is bound to oligo (dT)-cellulose and used as a template for reverse transcriptase, the oligo (dT) serving as a primer. After removing the template mRNAs, the cDNA-cellulose column is used to hybridize a population of mRNAs from induced cells. The non-hybridized poly A ⁺ RNAs are greatly enriched in the inducible sequences. We have used this technique …

History Of Animal Experimentation Control In The U.K., Judith E. Hampson

Laws and Legislation Collection

The legislative control of the use of animals in experiments in the UK lies in the Cruelty to Animals Act of 1876. Animal Welfare groups and individuals in Britain have pressed for reform of this law almost since its inception 105 years ago, and the British government has recently agreed to bring this legislation up to date. Any new or amended legislation could have far-reaching implications, both for laboratory animal welfare and upon the scientific community and is therefore of considerable importance both in this country and overseas. No proper appreciation of the problem would be possible without reference to …

Utilization Of Aspen Populus-Tremuloides Trees As A Ruminant Feed Component, M. Singh, L.D. Kamstra

Aspen Bibliography

When traditional roughages are in short supply or high in price, other fibrous feed sources for ruminants should be considered if available at a competitive price. One such fibrous material shown to have potential as a ruminant feed is the aspen tree (Populus tremuloides Michx.) harvested in its entire form to include bark, leaves and trunk. The aspen tree is the most widespread tree species in North America and the least utilized. Estimates exceed 6 million acres of mature trees in an area which would include the Black Hills (58,000 acres), the Great Lakes region and the Rocky …

Eye Movement Related Single-Unit Recording In The Nucleus Reticularis Tegmenti Pontis In The Alert Monkey, William Franklin Crandall Jr.

University of the Pacific Theses and Dissertations

Nucleus Reticularis Tegmenti Pontis (NRTP) is a structure which lies in the mid-brain ventral to the oculomotor complex. Anatomical studies strongly implicate it in oculomotor function as most of its inputs and outputs are to systems previously shown to be intimately involved in eye movement generation--superior colliculus (SC) and cerebellum. These reports show an integrative structure with inputs and outputs distributed in a way well suited for performing relay and feedback tasks. Physiological data in the alert, trained monkey has been gathered in an attempt to characterize these neurons and to classify them in functional terms. Three monkeys were trained …