Life Sciences Commons^™

Wildflower Of The Year; Mayapple (Podophyllum Peltatum), W. John Hayden

Biology Faculty Publications

Mayapples are perennial herbaceous plants with thick fibrous roots that arise from a creeping rhizome.

L-Theanine Modulates Stress Physiology And Immunology In Nile Tilapia, Sammie Miller, Lindee Mason, Shah Sumaiya Khaled, Elena Deng, Ahmed Mustafa

Mansoura Veterinary Medical Journal

Background: Fish are a prominent food source within the growing aquaculture industry, particularly within recirculating aquaculture systems, to alleviate global hunger demands. Diminishing detrimental stress conditions such as overcrowding prevalence and chemical toxins remain a primary goal. Nutraceuticals, particularly L-theanine, pose a natural alternative solution to alleviate these conditions. The amino acid L-theanine was scrutinized as a potential nutraceutical additive within commercial feed within the diet of Nile tilapia, Oreochromis niloticus, subjected to stress and unstressed in-vivo conditions.

Methods: To accomplish this, both 0.004% of body weight of L-theanine and 0.01% of body weight of hydrocortisone were implemented into feed …

Pharmacological Safety Profiling Of Nigella Sativa (Ns) And Hibiscus Sabdariffa (Hs) Extracts In Zebrafish (Danio Rerio) Embryos: An Integrative Vet-Pharmacotoxicological Evaluation Of Lc₅₀, Teratogenicity, And Developmental Outcomes, Ahmed E. A. Mostafa

Mansoura Veterinary Medical Journal

Herbal medicines are deeply rooted in Egyptian and Middle Eastern traditions, with Nigella sativa (NS) and Hibiscus sabdariffa (HS) being among the most widely consumed remedies. Despite extensive pharmacological evidence of their therapeutic benefits, systematic safety validation remains limited. Conventional toxicity assessments predominantly rely on rodents and higher vertebrates, which are costly, time-consuming, and ethically challenging. This gap necessitates alternative models that align with the 3R principles (Reduction, Replacement, Refinement). In this study, the zebrafish (Danio rerio) embryo model was employed to evaluate the developmental toxicity and median lethal concentration (LC50) of NS and HS aqueous extracts. Zebrafish embryos at …

Mental Fatigue Effect On Joint Repositioning, Collin Kehrwald

Dissertations and Theses

Mental fatigue influences both cognitive and physical performance, but its specific effects on proprioception have not been widely explored. This study aimed to examine how mental fatigue affects shoulder and knee joint repositioning senses. A joint repositioning task was used to assess proprioception in 18 college-aged males, with measurements taken for both shoulder and knee joint angles before and after a mental fatigue-inducing intervention. The Stroop task was employed to induce mental fatigue between the conditions. Results showed a significant increase in shoulder flexion variable error (p = 0.01, pre-Stroop = 2.79 ± 1.31 degrees, post-Stroop = 4.97 ± 3.20 …

Blattella Germanica As A Vector Of Salmonella Typhimurium, Matthew Turner

Dissertations and Theses

The following investigates the role of the German cockroach (Blattella germanica) as a vector of the bacterium Salmonella enterica subspecies enterica serovar Typhimurium (S. Typhimurium). As such, this work examines various host-pathogen-microbiome interactions that define the vectorial capacity of the German cockroach for Salmonella. To date, the majority of literature focuses on the general association of the German cockroach with various human pathogens, claiming any dissemination to be carried out via passive mechanical transmission. This project recognizes those claims, while expanding to include the possibility of biological transmission, involving active and complex host-pathogen interactions, including mechanisms like replication within the …

Small Mammal Diet As An Indicator Of Lyme Disease And The Road To Endemicity In Michigan’S Upper Peninsula, Jacqueline E. Suits

Dissertations, Master's Theses and Master's Reports

Lyme disease is expanding in the Upper Midwest due to climate change, forest degradation, and shifting wildlife communities. Small mammals are key reservoir hosts, and their foraging behavior offers insight into environmental conditions that sustain pathogen transmission. We used DNA metabarcoding to investigate small mammal diets across seasonal, spatial, and infection gradients in Michigan’s Upper Peninsula. We identified dominant dietary plant and arthropod taxa, seasonal shifts, and slight variation by species, site, and Lyme infection status. Taxa associated with Lyme-positive individuals were primarily linked to moist, edge, and disturbed habitats, areas that also support ticks and disease spread. These results …

Rotten Through The Cores: What Decomposition Can Tell Us About Carbon Cycling, Eileen C. Reeves

Dissertations, Master's Theses and Master's Reports

Sequestration of carbon (C) in forests means that the C is stored in the ecosystem and not emitted to the atmosphere, thus slowing the accumulation of greenhouse gases and mitigating the acceleration of manmade climate change. Quantifying the pools and fluxes of C in forests is therefore of considerable interest to landowners, policy makers, and scientists.

Decomposition is an ecological process central to the movement of C through ecosystems. This dissertation uses several techniques to help determine the factors influencing the speed of decomposition and long-term fate of decomposed plant material. In Chapter Two, phospholipid fatty acid analysis was used …

Understanding Trends In Zostera Research, Stressors, And Response Variables, Hannah R. Lyford, Emma Guerrini Romano, Shayna A. Sura, Sarah Joy Bittick

Biology Faculty Works

Background. Seagrass meadows are ecologically significant habitats that are globally threatened. Thus, there is increased interest in conservation of seagrasses as they face widespread decline. Biotic and abiotic factors that influence seagrass can be classified as stressors, such as rising temperature and eutrophication. Our study met an imminent need to consolidate data from previous studies to discern knowledge gaps and identify trends in studies, stressors, species, and geographic origination of research for the genus Zostera. For our systematic review, the objectives were to (A) qualitatively assess and summarize the current state of literature focused on seagrass species within the Zostera …

A Comprehensive Embryonic Staging Series Of The Turtle Trachemys Scripta, Christina M. Noravian, Perla G. Rand, Amira Mahomed, Maxellende Ezin, Annie Rickard, Oscar D. Juvera, Maria Elena De Bellard

Biology Faculty Works

Background: Turtles hold a unique place in vertebrate evolutionary history, making them critical assets in embryology research. Yet, they remain understudied as potential model organisms in the field. Here, to support experimental manipulations with turtle embryos, we have created a complete normal table of development for comprehensive embryonic staging of Trachemys scripta, one of the most common invasive turtle species worldwide.

Results: The development of T. scripta embryos from 0 days post-oviposition (DPO) to hatching (~60 DPO) was described from approximately 300 viable eggs collected at California State University, Northridge during the 2021–2024 nesting seasons. Thirty-one stages between oviposition and …

Nature, Nurture, And Serial Murder: A Biocriminological Analysis Of Jeffrey Dahmer And Aileen Wuornos, Dori W. Jal

Honors Thesis

In recent years, there has been an increase in public fascination with serial killers, however current criminological explanations continue to focus on social and psychological factors, lacking commentary on potential biological implications. This thesis addresses a gap in the literature by examining how biological processes, specifically epigenetic, neurobiological, and endocrinological mechanisms, interact with environmental stressors to shape serial homicidal behavior. The goal is to determine whether serial murder can be more effectively understood through a biosocial framework that integrates biological vulnerability with lived experience, rather than relying on singular explanatory models.

Using a qualitative multiple-case study methodology, this research analyzes …

Investigating Mitochondrial Stress Response Mechanisms In Aging, Aileigh E. Daniel

Theses and Dissertations

Mitochondrial dysfunction is widely recognized as a key contributor to aging and age-related diseases. The concept of mitohormesis suggests that low levels of ROS can activate stress-responsive transcription factors, promoting adaptive mechanisms that enhance longevity. We hypothesized that changes in protein abundance under conditions of mild mitochondrial stress reflect an adaptive response, and that commonly altered proteins across diverse genetic backgrounds of mitochondrial stress can help identify key regulators of stress resilience and aging. Our study identified conserved mitochondrial stress response pathways and highlighted candidate regulators for further investigation. These regulators appear to impair mitochondrial energy production by downregulating ATP …

Unleashing Serine Palmitoyltransferase In Test Tubes And Mice, Matthew W. Peart

Theses and Dissertations

Canonically known both for structural contributions to lipid bilayers and roles in cell signaling, the sphingolipids comprise a dynamic, multifaceted class of molecules which are studied to understand homeostatic cell biology as well as pathophysiology. Sphingolipids contribute to tissue-specific structures such as myelin, a multilayer membrane that allows for rapid conduction of action potentials along neuronal axons. All sphingolipids are downstream products of the rate-limiting and initiating enzyme in the de novo sphingolipid synthesis pathway, Serine palmitoyltransferase (SPT). Demonstrative of its status as the enzyme that catalyzes a crucial step in a biochemical pathway, SPT activity is strictly regulated. This …

Climate Change-Induced Shifts In Mediterranean Fungal Fruiting Phenology And Productivity, Rachel Demay

Theses and Dissertations

We used a 29-year dataset of mushroom fruiting from the Pinar Grande forest in northern Spain to investigate the effects of climate change on the phenology, productivity, and diversity of mycorrhizal and saprotrophic mushrooms. We investigated the long-term trends in fruiting season length, yield (by count, total weight, and average weight), and genus richness. Additionally, we identified associations between monthly temperature and monthly precipitation with these fungal responses across functional groups: mycorrhizal, saprotrophic, litter-decaying saprotrophic, and wood-decaying saprotrophic mushrooms, and the economically-important edible genera Boletus and Lactarius. We found that autumnal season length in the Pinar Grande has shortened over …

Molecular Mechanisms Of Kynurenine-Mediated Lifespan Regulation In Yeast, Ryan E. Counterman Jobb

Theses and Dissertations

The kynurenine pathway is a highly conserved pathway that acts as the primary pathway for tryptophan catabolism and has been found to be involved in lifespan regulation across many diverse organisms. Caloric restriction (CR) is the only known dietary intervention to date that both increases the mean lifespan and the health span of multiple organisms, although the molecular mechanisms of these effects are not fully understood. There are known and sometimes contradictory relationships between the kynurenine pathway activity and the life- extending effect of CR but the crosstalk between the two is not well characterized. Understanding these relationships could lead …

Evaluation Of The Impact Of Secondhand E-Cigarette Aerosols On Ros-Mediated Alterations Of Epigenetic Events: Implications For Copd Pathogenesis And Assessment Of The Efficacy Of Cold Atmospheric Plasma In Degrading Organophosphate Pesticides On Kevlar Materials, Ta-Chun Lin

Masters Theses

"We explored 1) the toxicological impacts of e-cigarette aerosols and 2) the decontamination efficacy of Cold Atmospheric Plasma (CAP) technology over organophosphates. In examining the effects of firsthand and secondhand e-cigarette aerosol exposure on A549 and BEAS-2B lung cells, we observed significant cytotoxicity, increased ROS levels, and epigenetic modifications, including heightened DNA methylation and downregulation of histone deacetylases (HDACs). These molecular alterations may contribute to chronic respiratory conditions, such as COPD, underscoring the health risks of e-cigarette exposure.

We investigated CAP’s effectiveness in degrading organophosphate pesticide (OP) residues on fabric surfaces, revealing that CAP treatment effectively decomposes OPs such as …

Bacteria-Engineered Vesicles For Cancer Immunotherapy: From Immunomodulation In Vitro To Anti-Tumor Effects In Melanoma Models, Lan Li

Theses and Dissertations--Chemistry

Bacterial vesicles hold immense potential in various biomedical fields. Among these, outer membrane vesicles (OMVs) produced by Gram-negative bacteria are the most extensively studied. While the exact mechanism of OMV production remains unclear, numerous environmental factors have been shown to influence both their yield and composition. In this study, we investigated the effect of three different antimicrobial families on OMV production by E. coli. Interestingly, antimicrobials within the same family did not provide the same effects on OMV yield, suggesting that OMV production may not directly correlate with the antimicrobial mechanism of action. OMVs have demonstrated tumor-inhibitory activity in multiple …

Functional And Structural Impacts Of Bison Grazing Intensity On Plant Communities, Samantha Lynn Berk

Graduate Research Theses & Dissertations

The reintroduction of grazing disturbance has become an increasingly popular tool in grassland restoration to build and maintain diverse and functioning ecosystems, but our understanding of how grazers like bison (Bison bison) shape plant communities is limited by binary grazing treatments and unanswered calls for in-depth and mechanistic examinations of community function and structure. Little research has focused on how natural variations in bison grazing intensity impact the function and structure of restored tallgrass prairie; whereby functional diversity is purely defined by changes in trait expression according to plant responses to grazing, and examining both vegetation structure and ecosystem function …

"On The Mat They Will Get Treated The Same": Male High School Wrestlers' Attitudes Towards Female Opponents, Emily Ann Kaht

Graduate Research Theses & Dissertations

Wrestling has historically been considered a male sport. Until recently, so few girls wrestled that those who did had no choice but to take on male opponents. Media reports and the literature indicate that those girls faced a wide range of attitudes from their opponents. While some women reported feeling supported by their male teammates, others reported hostility. Some stakeholders have, in the past, argued that wrestling is inherently unsuitable for girls, and some girls had trouble securing matches due to widespread refusal of boys to wrestle against them. Reasons given for boys forfeiting against girls have included a fear …

Redox Control In A Fused Electron Transfer Flavoprotein From A Thermophilic Archaeon And Expanded Significance Of A Hydrogen Bond From A Conserved Histidine Residue That Contributes To Flavin Redox Tuning And Activation For Covalent Modification, Debarati Das

Theses and Dissertations--Chemistry

In absence of O₂ as terminal electron acceptors in anaerobic bacteria and archaea, carbohydrate metabolism is 15 times less efficient compared to aerobic energy metabolism resulting in energy deficit conditions. Despite their meager resources these anaerobes were able to generate H₂ and a chemiosmotic potential able to drive energy demanding reactions such as CO₂ or N₂ fixation. These observations raised concerns as production of high energy reductants (H₂) from mediocre fuels (NADH) defied the laws of thermodynamics.

In 2008, a known mechanism “electron bifurcation” but with flavins as redox mediators instead of quinones was …

Evaluation Of Blue Catfish Populations In Four Midwestern Power Cooling Reservoirs, Tyler Joseph Murray

Masters Theses

Blue Catfish (Ictalurus furcatus) are the largest catfish in North America and as such angler desire for trophy fisheries have grown in addition to harvest fisheries. The Illinois Department of Natural Resources (IDNR) has stocked Blue Catfish into several reservoirs in the state including four that have powerplants that utilize the water for cooling and discharge it back into the reservoir at increased temperatures. This study explored the potential impacts of these warm water effluents on Blue Catfish population demographics, as well as whether management changes need to be implemented in these systems. I sampled Powerton, LaSalle, Springfield, …

Soils From Different Solidago Altissima Clones Mediate Intraspecific Interactions In Schizachyrium Scoparium, Sarah Alharbi

Masters Theses

While previous studies have examined plant intraspecific competition and soil microbial effects separately, fewer studies have investigated how these factors interact, particularly in the context of genotype-specific soil conditioning effects. Plant-soil feedbacks can significantly alter interspecific competitive outcomes, yet few studies have examined how local variation in soil conditioning influences plant responses to density-dependent competition. I investigated how soil inocula derived from four Solidago altissima clones that were previously shown to differ in their soil conditioning effects (Foster et al., 2022) affected the growth and root traits of Schizachyrium scoparium across a gradient of intraspecific competition (1, 2, or 4 …

Barriers To Leisure-Time Physical Activity Among College Students, Ben Andrew Winfield

Masters Theses

Obesity is an increasing problem worldwide. In general, people eat more and exercise less, with a lot of individuals not meeting World Health Organization recommendation of 150-300 minutes of moderate-intensity physical activity or 75-150 minutes of vigorous-intensity physical activity weekly. At times, individuals may face barriers which prevent or inhibit their ability to participate in leisure-time physical activity (LTPA), exercise completed in one’s free time. These barriers are known as leisure constraints and are comprised of three categories: intrapersonal, feelings and psychological aspects; interpersonal, relationship aspects; and structural, physical aspects. Body image, the perception individuals have about their bodies and …

Optimization Of Transformation And Regeneration In The Sunflower (Helianthus Annuus), Sara M. Kilar

Masters Theses

In addition to their value as prized ornamentals, sunflowers are important crop species due to the nutritional value and high oil content of the seeds. Sunflower oil is a significant contributor to global seed oil production, which can be used as a food additive and cooking agent as well as a raw material for biodiesel production. While traditional breeding efforts have led to the development of many unique cultivars, genetically modified variations have proven more difficult due to the recalcitrance of sunflower plants to genetic transformation. As a result, facile and efficient transformation methods for sunflowers have the potential to …

Influence Of Thermophilic Biofilms On Proliferation Of Opportunistic Pathogens In Domestic Water Systems, Olajumoke Akinbami

Masters Theses

Domestic hot-water systems quietly grow biofilms under elevated temperatures that can serve as shelter to Opportunistic Premise Plumbing Pathogens. Although focus has been placed on microbes in domestic systems, there has been no clear conclusion on the mechanism of survival and persistence of opportunistic pathogens despite various disinfection strategies. In this study, we determined the distinction between showerhead and drip-flow biofilms at the whole-community level and analyzed the coexistence of thermotolerant organisms with opportunistic genera, such as Legionella. We sampled eight showerheads and simulated four biofilm samples in the laboratory using the drip-flow biofilm reactor.

We observed a strong …

Insect Foraging Behaviors Across Habitat Types In Weddell’S Saddle-Back Tamarins, (Leontocebus Weddelli Weddelli) In Pando, Bolivia, Brianna Nicole Abba

Graduate Research Theses & Dissertations

Researchers assume that small-bodied primates like the callitrichines are highly adaptable to environmental change and can increase their consumption of insects in disturbed habitats, however, these assumptions have rarely been tested. To better understand how habitat and other factors may influence the frequency of insect foraging, the type of prey capture techniques used, and prey capture success, I collected detailed observational data on one group of Weddell’s saddle-back tamarins (Leontocebus weddelli weddelli) in northwestern Bolivia in July and August 2022. I found that tamarins spent 62% of their total foraging and feeding time on insects. They spent 89% of their …

Gustatory Behaviors Of House Flies: Body Parts Used And Response To Bitter, R M Panchalie Bhagya Gunathunga

Graduate Research Theses & Dissertations

Insects use multiple body parts, not just their mouth, to taste. Whether a particular body part has a taste function can be determined indirectly by examining the morphology or electrophysiological response of its sensilla (sense organs) or directly by the insects’ behavioral response. Understanding the taste system of insects allows development of better management strategies against pest insects and strategies to protect beneficial insects.House flies, Musca domestica L., are pests at livestock facilities. House fly numbers are controlled mainly by manure management and chemical controls. Toxic granular fly baits remain one of the most popular and effective forms of chemical …

Geology, Paleoecology And Vertebrate Biostratigraphy Of The Hell Creek Formation, Carter County, Montana, Usa, Joshua Collin Mathews

Graduate Research Theses & Dissertations

The Hell Creek Formation of the Northern Great Plains records one of the greatest mass extinction events in Earth’s history, bringing to a close the reign of the dinosaurs at the end of the Cretaceous period. This rock unit comprises one of the most studied geological formations in the world and is one of the few locations on the planet that preserve terrestrial deposits containing ejecta from the bolide impact that slammed into the Yucatan Peninsula 66 million years ago. This profound event in the geologic record has led to numerous studies of paleobiotas, climate change, ecosystem recovery dynamics, and …

Alternative Mechanisms For The Control Of House Flies, Elizabeth Taylor

Graduate Research Theses & Dissertations

This dissertation examines three alternative mechanisms for the control of house flies, Musca domestica L., a cosmopolitan filth fly pest common in agricultural and urban areas where decaying organic matter is available for larval development. Adult M. domestica are nuisances and vectors of disease. When sanitation, screens, and sticky fly strips fail to control house fly populations, insecticide baits are often used. However, the use of baits can lead to the evolution of resistance, including not only physiological resistance to the toxicant but also behavioral resistance to the phagostimulant and to the toxicant. This evolution of resistance to phagostimulants and …

Establishing A Microfiber Recovery Rate In Human Lung Tissue, Kylie Nicole Babin-Howard

Graduate Research Theses & Dissertations

Microplastics (plastic particles smaller than 5mm) are of increasing concern to environmental and human health as they have persistently been found in all areas of study—becoming a ubiquitous part of the planet. Microplastics have been found in a variety of human tissues (lungs, blood, brain matter, etc.) yet their health effects are poorly understood, with experimental exposure model studies indicating adverse health effects. Microfibers have been found to be more toxic when compared to other microplastic shapes and are also shed from a majority of everyday items, with environmental reports showing that fibers are more than twice as prevalent than …

Development And In Vitro Evaluation Of Novel Near-Ir Fluorescent Sensors For Egfr Kinase, Haniyeh Jadidi

Graduate Research Theses & Dissertations

This thesis presents the in vitro evaluation of a novel near-infrared (NIR) fluorescent small-molecule probe AB-139 that selectively binds to epidermal growth factor receptor (EGFR) kinase and operates through a turn-on fluorescence mechanism. The sensor addresses several limitations associated with conventional protein-based probes by offering small-molecule specificity, tunable modularity, deep-tissue imaging potential, and reduced background noise. AB-139 comprises four key components: A Zn-phthalocyanine fluorophore that serves as the NIR emission reporter; hydrophilic triethylene glycol chains that enhance aqueous solubility and reduce aggregation; cationic quaternary ammonium groups that facilitate cellular permeability; and a gefitinib-derived recognition anchor that targets the intracellular ATP-binding …