Life Sciences Commons^™

The Chemical Ecology Of A Caribbean Boulder Coral During Restoration And The Interplay Between Fish Predation And Microbiome Variation, Catherine E. Raker

Open Access Dissertations

Coral reefs are one of the most biodiverse and productive ecosystems on the planet. In recent decades, however, climate change and other global threats have caused severe declines in live coral cover worldwide. Coral propagation, or “gardening,” is a popular strategy to attempt to restore healthy corals to degraded reefs. Slow-growing boulder corals are often restored using microfragmentation, where cutting coral colonies into roughly 1 cm² fragments can increase their growth rates up to 5x. However, extreme early mortality of fragments has been observed in the field, which has been at least partially attributed to predation. It has been …

Fluke Egg Sedimentation Test Procedure, Department Of Primary Industries And Regional Development, Western Australia

Animal production and livestock factsheets

The department’s approved procedure for detecting trematode eggs and the Eimeria leuckarti sedimentation method (FEST) on faecal samples.

Skeleton Weed In Western Australia: 2025 Management Guide, Department Of Primary Industries And Regional Development, Western Australia

Biosecurity bulletins

Skeleton weed can reduce crop yields by competing for moisture and nutrients (mainly nitrogen).

This guide helps you manage and eradicate skeleton weed infestations on your property, and helps to stop the spread across WA.

Without the coordinated program aimed at controlling the spread, skeleton weed would now be much more abundant and widely established throughout cereal growing areas.

If you find skeleton weed on your property, we can help you get rid of it.

Skeleton weed is unpredictable and can spread over long distances due to wind dispersal, but once identified it can be managed effectively and eradicated

Skeleton Weed In Western Australia: Control Program 2024–2025, Department Of Primary Industries And Regional Development, Western Australia

Biosecurity bulletins

Key program changes

Winter eradication program

New herbicide recommendations for control in crops, legume based annual pastures, plantations and glyphosate tolerant canola.

• The minimum size of ‘squares’ (infested areas) has been reduced from 40 m x 40 m (0.16 ha) to 20 m x 20 m (0.04 ha) to help reduce the winter treatment area.
• Landholders will need to consult with DPIRD/LAG officers to assess the degree of infestation before working or cultivating through squares in Code 1 paddocks.
• Clopyralid (LontrelTM) herbicide will no longer be provided for treatment on heavily infested paddocks. Landholders will still be required to undertake …

Little Lives, Big Challenges: The Nicu's Role In Infant Brain Development, Gerardo Martinez

Scientific Kenyon: The Neuroscience Edition

No abstract provided.

Sleep Is Paralyzing Me! Exploring The Brain During Sleep Paralysis, Anna Renteria

Scientific Kenyon: The Neuroscience Edition

No abstract provided.

Dopamine Hits To Data Bits: Harnessing Brain Chemistry To Drive The Next Generation Of Computing, Keller Bueneman

Scientific Kenyon: The Neuroscience Edition

No abstract provided.

Oops! My Brain Did It Again! How Music, Smell, And Sight Rekindle The Past, Lana Hunt

Scientific Kenyon: The Neuroscience Edition

No abstract provided.

The Anatomy Of Art: Visual Art And The Brain, Shea Wilt

Scientific Kenyon: The Neuroscience Edition

No abstract provided.

Saying "Goodbye" To The Deficits Of Spinal Cord Injury: Exploring The Inflammatory Mechanisms Underlying Spinal Cord Injury And Related Mood Disorders, Elsie Groebner

Scientific Kenyon: The Neuroscience Edition

No abstract provided.

Investigating Relatedness Within Breeding Units Of The Highly Social Bushtit (Psaltriparus Minimus), Fiona Skye Connelly

Theses and Dissertations--Biology

Cooperative breeding systems represent an array of varied reproductive strategies and remain a topic of interest in behavioral ecology (i.e., Riehl 2013, Cornwallis et al. 2017). Different manifestations of cooperative breeding, such as cooperative polyandry, no doubt have unique evolutionary pressures tied to local ecologies which have led to their proliferation. Of particular interest are species that show flexibility in their use of reproductive strategies, wherein individuals may choose one strategy over another given a specific circumstance. Here, I used double-digest restriction site-associated DNA sequencing (i.e., ddRAD-seq) to investigate bushtit (Psaltriparus minimus) reproductive strategies and the relatedness between …

Temporal Dynamics Of Programmed Genome Rearrangement In Sea Lamprey Embryogenesis: A Deep Learning & Lightsheet Imaging Approach To Studying Early Developmental Transitions, Kasturi Rajandran

Theses and Dissertations--Biology

Programmed genome rearrangement (PGR) in the sea lamprey (Petromyzon marinus) leads to the elimination of ~0.5Gb (20%) of the germline genome during early embryogenesis, yet its timing relative to the maternal-to-zygotic transition (MZT) remains unclear. In this study, we imaged embryos cleared at 3-hour intervals (up to 72 hours post fertilization (hpf)) using high-resolution lightsheet fluorescence microscopy and a custom deep-learning image-analysis pipeline (~80% accuracy) to quantify nuclei and micronuclei counts. Micronuclei, which are the hallmarks of PGR first appear between 24 to 27hpf (vs ~36hpf previously reported) indicating PGR starts earlier than previously reported in the sea …

The Role Of Metabolic Gate Keepers In Cell Fate Decision Making, Brian Kelly

Theses and Dissertations--Biology

Maintaining cellular energy balance and redox stability is crucial to cell survival, with glutamine metabolism playing a central role. While extensively studied in cancer, where it supports biosynthesis and reduces oxidative stress to promote tumor growth, its function in non-cancerous contexts like tissue development and muscle regeneration remains less understood. A key aspect of stem cell biology is quiescence, a metabolically dormant state that preserves the stem cell pool, yet the mechanisms regulating this state are not fully clear. Here, we identify glutamine metabolism as a critical factor governing myoblast proliferation and quiescence through a metabolic program distinct from that …

Does Time Actually Fly When You're Having Fun? A Review Of Time Perception In Differing Environments, Arthur Khayat

Scientific Kenyon: The Neuroscience Edition

No abstract provided.

Ai Think, Therefore, Ai Am?, Bineth Abeysekera

Scientific Kenyon: The Neuroscience Edition

No abstract provided.

Please, Put Away That Plastic! A Plea From Your Grandchildren, Finn Anders

Scientific Kenyon: The Neuroscience Edition

No abstract provided.

Unlocking Grit And Mindfulness: Biohacking The Anterior Mid-Cingulate Cortex For Success, Rowan Goldin

Scientific Kenyon: The Neuroscience Edition

No abstract provided.

Extracellular Vesicles Released By All Patients Contain Hne-Adducted Proteins: Implications Of Collateral Damage, Jenni Ho, Suriyan Sukati, Tamara Taylor, Sherry Carter, Brittany Fuller, Amy Marmo, Caryn Sorge, John A. D'Orazio, D. Allan Butterfield, Subbarao Bondada, Heidi Weiss, Daret K. St. Clair, Luksana Chaiswing

Markey Cancer Center Faculty Publications

Off-target neuronal injury is a serious side-effect observed in cancer survivors. It has previously been shown that pediatric acute lymphoblastic leukemia (ALL) survivors have a decline in neurocognition compared to healthy age-matched counterparts. Elevated oxidative stress has been documented to be a mediator in off- target tissue damage in cancer survivors. Early detection of oxidative stress markers may provide an opportunity to prevent off-target tissue damage. Extracellular vesicles (EVs) have surfaced as a potential diagnostic tool due to molecular cargo they contain. We investigated the potential for EVs to be a sensitive indicator of oxidative stress and off-target tissue damage …

Pharmacological Or Genetic Inhibition Of Ltcc Promotes Cardiomyocyte Proliferation Through Inhibition Of Calcineurin Activity, Lynn A. C. Devilée, Abou Bakr M. Salama, Jessica M. Miller, Janice D. Reid, Qinghui Ou, Nourhan M. Baraka, Kamal Abou Farraj, Madiha Jamal, Yibing Nong, Todd K. Rosengart, Douglas A. Andres, Jonathan Satin, Tamer M. A. Mohamed, James E. Hudson, Riham R. E. Abouleisa

Markey Cancer Center Faculty Publications

Cardiomyocytes (CMs) lost during ischemic cardiac injury cannot be replaced due to their limited proliferative capacity. Calcium is an important signal transducer that regulates key cellular processes, but its role in regulating CM proliferation is incompletely understood. Here we show a robust pathway for new calcium signaling-based cardiac regenerative strategies. A drug screen targeting proteins involved in CM calcium cycling in human embryonic stem cell-derived cardiac organoids (hCOs) revealed that only the inhibition of L-Type Calcium Channel (LTCC) induced the CM cell cycle. Furthermore, overexpression of Ras-related associated with Diabetes (RRAD), an endogenous inhibitor of LTCC, induced CM cell cycle …

The Adiponectin-Pparγ Axis In Hepatic Stellate Cells Regulates Liver Fibrosis, Shangang Zhao, Qingzhang Zhu, Wang-Hsin Lee, Jan-Bernd Funcke, Zhuzhen Zhang, May-Yun Wang, Qian Lin, Bianca Field, Xue-Nan Sun, Guannan Li, Mbolle Ekane, Toshiharu Onodera, Na Li, Yi Zhu, Christine M. Kusminski, Terry D. Hinds Jr., Philipp E. Scherer

Markey Cancer Center Faculty Publications

Hepatic stellate cells (HSCs) are key drivers of local fibrosis. Adiponectin, conventionally thought of as an adipokine, is also expressed in quiescent HSCs. However, the impact of its local expression on the progression of liver fibrosis remains unclear. We recently generated a transgenic mouse line (Lrat-rtTA) that expresses the doxycycline-responsive transcriptional activator rtTA under the control of the HSC-specific lecithin retinol acyltransferase (Lrat) promoter, which enables us to specifically and inducibly overexpress or eliminate genes in these cells. The inducible elimination of HSCs protects mice from methionine/choline-deficient (MCD) diet-induced liver fibrosis, confirming their causal involvement in fibrosis development. We generated …

Urobilin Derived From Bilirubin Bioconversion Binds Albumin And May Interfere With Bilirubin Interacting With Albumin: Implications For Disease Pathology, Kevin I. Williams, Priyanka Suryadevara, Chang-Guo Zhan, Terry D. Hinds Jr., Zachary A. Kipp

Markey Cancer Center Faculty Publications

Background/Objectives: Bilirubin is a hydrophobic molecule that binds the carrier protein albumin for transport through systemic circulation. Bilirubin is cleared from the body through the liver and excreted into the intestines, where the microbiota modifies the chemical structure, forming urobilin, which can be reabsorbed into circulation by the hepatic portal vein. Urobilin has no known function. It is also unknown whether urobilin binds albumin for transport in circulation. We hypothesized that because of the likeness of their chemical structures, urobilin would also bind albumin like bilirubin does. Methods: First, we used in silico docking to predict if urobilin would bind …

Dissecting The Biophysical Mechanisms Of Oleate Hydratase Association With Membranes, William A. Lathram, Robert J. Neff, Ashley N. Zalla, James D. Brien, Vivekanandan Subramanian, Christopher D. Radka

Markey Cancer Center Faculty Publications

This study investigates the dynamics of oleate hydratase (OhyA), a bacterial flavoenzyme from Staphylococcus aureus, and its interactions with lipid membranes, focusing on the factors influencing membrane binding and oligomerization. OhyA catalyzes the hydration of unsaturated fatty acids, playing a key role in bacterial pathogenesis by neutralizing host antimicrobial fatty acids. OhyA binds the membrane bilayer to access membrane-embedded substrates for catalysis, and structural studies have revealed that OhyA forms oligomers on membrane surfaces, stabilized by both protein-protein and protein-lipid interactions. Using fluorescence correlation spectroscopy (FCS), we examined the effects of membrane curvature and lipid availability on OhyA binding to …

Mir-27a-3p Regulates Intestinal Cell Proliferation And Differentiation Through Wnt/Β-Catenin Signalling, Chang Li, Yuning Zhou, Yinping Jiang, Zhijie Yin, Heidi L. Weiss, Qingding Wang, B. Mark Evers

Markey Cancer Center Faculty Publications

Intestinal stem cells differentiate into absorptive enterocytes, characterised by increased brush border enzymes such as intestinal alkaline phosphatase (IAP), making up the majority (95%) of the terminally differentiated cells in the villus. Loss of integrity of the intestinal epithelium plays a key role in inflammatory diseases and gastrointestinal infection. Here, we show that the intestinal microRNA (miR)-27a-3p is an important regulator of intestinal epithelial cell proliferation and enterocyte differentiation. Repression of endogenous miR-27a-3p leads to increased enterocyte differentiation and decreased intestinal epithelial cell proliferation in mouse and human small intestinal organoids. Mechanistically, miR-27a-3p regulates intestinal cell differentiation and proliferation at …

Characterizing Agroecosystem Effects Of Industrial Hemp On A Rotational Row Cropping System And Microbial Communities, Kent D. Pham

Theses and Dissertations--Plant and Soil Sciences

The implementation of crop rotation on a farm can have many benefits for agroecosystem health and production. This practice can potentially reduce nutrient amendments, disrupt pest and disease cycles, and increase crop yields. In Kentucky, one common rotation consists of either corn or soybean grown the first year, followed by the other crop, repeated every two years. Industrial hemp, grown for fiber or grain, is an emerging crop that has shown promise as a rotation alternative, although not much is known about its effects on soil health, agroecosystem functioning, and microbial communities. To better characterize hemp’s role within American agriculture, …

Factors Impacting Germination, Emergence, And Establishment Of Direct Seeded And Transplanted Hemp (Cannabis Sativa L.), Paul Cockson Dr.

Theses and Dissertations--Plant and Soil Sciences

Industrial hemp (IH) establishment has many challenges including: poor emergence, low stand establishment, and variable plant development in direct seeded hemp. In floral hemp production, quality transplant production is a challenge with seedlings often becoming root bound and etiolated. In both direct seeded and transplant hemp, the relationship between root vigor and vegetative growth has not been studied and may prove insightful for managing emergence and establishment challenges. The goals of this study were three-fold: 1) to measure and describe root system architecture (RSA) and root phenotypes observed in selected IH cultivars especially in regard to identifying agronomically important RSA …

Evaluation Of Poaceae Species And Suppression Application Methods For Perennial Groundcover In Corn, Hallie A. Sandeen

Theses and Dissertations--Plant and Soil Sciences

Intensive grain production aims to provide food security but has depleted once-perennialized land. Perennial groundcover (PGC) offers ecosystem services to intensive annual corn (Zea mays L.) production. In past research conducted in the U.S. Midwest, Kentucky bluegrass (Poa pratensis L.) has been the standard PGC species. However, it is slow growing and difficult to suppress. To characterize the PGC system in the midsouth, two separate field trials in Lexington, Kentucky, were conducted to (i) compare the first-year establishment of alternative cool-season species to Kentucky bluegrass, with and without a spring-applied broadleaf herbicide, and their impact on corn grain …

Examining The Distribution And Predictability Of Population Dynamics Across Geographic Space, Cleber Ten Caten

Theses and Dissertations

Populations occupy different regions of the geographic space and may experience distinct conditions over time that can cause fluctuations in their number of individuals. Environmental conditions, dispersal events, and resource availability are some of the factors that influence populations. A central goal in ecology has been to develop generalizations about how these factors that affect population dynamics are structured in geographic space. However, limited knowledge about where species occur and a lack of long-term surveys of populations has hindered the development, and validation, of such generalizations. In this dissertation, I use a combination of observational data, simulations, and experimental approaches …

The Everyday Scientist: Down In The Weeds In The Heart Of The South, Pamela Zendt

Master's Projects

The Everyday Scientist: Down in the Weeds in the Heart of the South is a creative nonfiction capstone project that was released as a book. The narrative documents my two-year journey as a community scientist and explores how ordinary people contribute meaningfully to scientific research in the natural world. Rooted in my desire to connect my passion for the outdoors with the goals of the Master of Arts in Professional Writing program, this project illustrates that environmental science is not only for trained professionals. Instead, it thrives on the curiosity, dedication, and participation of volunteers who follow established protocols, collect …

Effects Of Mental Fatigue On Gait Performance And Variability, Ali Zardosht

Dissertations and Theses

Mental fatigue has been shown to negatively impact physical performance, including motor skills and neuromuscular function. This study aimed to investigate the effects of mental fatigue on gait performance and variability in healthy young male adults. Eighteen participants walked over-ground at their preferred speed before and after completing a mentally fatiguing Stroop task. Kinematic data were recorded to assess gait variables such as speed, step length, step time, and step width, along with their coefficients of variation (CV). While subjective ratings of mental fatigue significantly increased following the Stroop task, no significant changes were observed in gait performance or CV …

Integrated Pest Management: State Infrastructure Status After 50 Yr Of Federal Support (1973 To 2023), Daren S. Mueller, Laura C. Iles, Carol L. Pilcher, Adam J. Sisson, Roger Magarey, Ryan Adams, Wanda I. Almodovar, Diane Alston, Patrick Beauzay, Ricardo Bessin, Mandy Bish, Mary Burrows, Alejandro Calixto, Rakesh Chandran, Jed B. Colquhoun, Mary Concklin, Amy J. Dreves, Peter C. Ellsworth, Paul D. Esker, James J. Farrar, Alfred Fournier, Daniel Frank, Kelly Hamby, George Hamilton, Anthony Hanson, Ann Hazelrigg, Natalie Hein-Ferris, David Held, James Jasinski, Heather M. Kelly, David Kerns, Miranda Kersten, Lauren Kerzicnik, Janet Knodel, Glen Koehler, Heidi Kratsch, Christian H. Krupke, Norman C. Leppla, Erin Lizotte, Casey Matney, Rebecca A. Melanson, Frannie Miller, Marion Murray, David Owens, Diane Plewa, Francis P. F. Reay-Jones, Silvia I. Rondon, Tom A. Royer, Philip A. Rozeboom, Hilary A. Sandler, Scott P. Schell, Marissa Schuh, Timothy Seipel, Danesha Seth Carley, Ashfaq Sial, Raghuwinder Singh, Damon L. Smith, Tim Stock, Glenn Studebaker, Adrianna Szczepaniec, Lisa Tewksbury, John Tooker, Adam J. Varenhorst, Amber Vinchesi-Vahl, Doug Walsh, Desiree Wickwar, Robert J, Wright, Simon Zebelo

Department of Entomology: Faculty Publications

Integrated pest management uses a variety of tools coupled with historical, current, and projected information for economical pest protection of crops and other resources while accounting for risk to humans and the environment. Following the 1972 US Federal IPM Policy, funding for integrated pest management programming has continued for 50+ years. However, multifaceted changes during this time have significantly affected state-level integrated pest management infrastructure, prompting a comprehensive survey to assess conditions, limitations, and growth potential of US integrated pest management programs. A survey was sent to 50 US states and 3 territories with integrated pest management programs in November …