Life Sciences Commons^™

Are There Neurological Impacts Of Exposure To A Burned Wildfire Site As It Recovers?, Rubye Strickland

Undergraduate Honors Theses

Wildfires are increasing with climate change, making the human health implications of exposure to burned soils an essential area of research. Metals associated with fire-impacted soils can be inhaled or accidentally ingested by surrounding communities, causing health risks. Several studies have evaluated the health impact of wildfires, but few have done so from a neurological perspective. This study explores metal changes in burned soils over time as a site naturally recovers from wildfire and how these changes may impact human neurological health. Soil samples were collected from the burned area 0, 3, 6, 9, and 12 months after the fire. …

Food Security And Its Impact On Hypertension In Urban Communities, Lauren M. Nobriga

Master's Projects and Capstones

The Quality Improvement Team proposed an intervention to increase enrollment in the Food Security Program, a six-month initiative that provides vouchers redeemable for fruits and vegetables. Long-term impacts of this Program include an improved food economy and enhanced patient disease management autonomy. This project aimed to increase the percentage of eligible adult medicine clinic patients enrolled in the Food Security Program by 25%, or 11 patients per month, based on the mean enrollment for the first four months of the Program (May to August) of nine patients. The intervention was based on bi-monthly performance reports, making the goal include enrolling …

Effects Of Microbial Lipases On Parmesan And Feta Cheese Flavor Profiles, Erin N. Caswell

Theses and Dissertations

Lipolysis is crucial for cheese flavor development, as lipolytic enzymes convert fats into free fatty acids that enhance flavor. While Kid/Goat (KG) lipases are traditionally used in cheese production, microbial lipases like M. javanicus (MJ) and C. cylindracea (CC) provide vegetarian, cost-effective alternatives. This study compares the flavor, texture, and composition of Feta and Parmesan cheeses made with microbial lipases as a replacement for traditional lipase. Conventional cheese making techniques were used to make experimental batches of Feta and Parmesan cheeses using a standard KG lipase and two experimental lipases, CC and MJ, and comparing all three against a control …

Climate Change Impacts On Dryland Ecosystems, Tanner Tainui Aiono

Theses and Dissertations

Dryland ecosystems are some of the most prevalent ecosystems on Earth and have recently shown stress indicators from climate change. These water-limited systems are vulnerable to increases in temperature and changes in precipitation, especially when looking at carbon cycling dynamics across biological sol crusts and vegetation mortality patterns. This dissertation aims to 1) evaluate how precipitation dynamics, vegetation location, and microclimate conditions influence biological soil crust carbon conditions, and 2) evaluate how topographic influences drive patterns of plant mortality during prolonged drought on the Colorado Plateau. Using high-resolution monitoring of long-term NEE chambers, we found that intermediately disturbed biocrusts are …

Identification Of Salt Tolerance And Stress Response In Us Department Of Agriculture Tomato Germplasm At The Seedling Stage, Ibtisam Alatawi, Haizheng Xiong, Kenani Chiwina, Hanan Mohammedsaeed Alkabkabi, Qun Luo, Neelendra K. Joshi, Xiaolun Sun, Xuan Zhuang, Kai-Shu Ling, Ainong Shi

Horticulture Faculty Publications and Presentations

Abstract. Soil salinity is a significant abiotic factor that impedes sustainable crop pro- duction in key agricultural regions worldwide. Saline cultivation adversely affects soil quality, whereas the use of saline water for irrigation disrupts the physiological and biochemical processes of plants. Continuous irrigation with high salt concentrations leads to a gradual buildup of soil salinity, thus hindering optimal plant growth and development. Consequently, there is a growing emphasis on breeding salinity-tolerant cultivars of various crops. In this study, 71 tomato accessions sourced from 20 coun- tries and provided by the US Department of Agriculture were evaluated under con- trolled greenhouse …

Improving Food Security To Decrease Hypertension At An Urban Community Clinic, Christine S. Villalta

Master's Projects and Capstones

Diet and lifestyle changes have been proven to reduce blood pressure in patients with hypertension, and a key dietary approach aligned with this recommendation is the DASH diet, which includes consuming fruits and vegetables. Food insecurity, defined as lacking enough safe, nutritious food to maintain an active and healthy life, is a growing crisis in the United States, affecting 13.5% of U.S. households in 2023. This is especially significant for those whose chronic disease management depends on the accessibility of healthy food. An urban community clinic implemented a Food Security Program to decrease food insecurity and reduce hypertension among their …

Using Genomics And Transcriptomics As A Tool To Understand Unique Phenotypes Of Hemipteran Insects That Are Relevant To Agriculture And Public Health, Hunter Kelly Walt

Theses and Dissertations

In the past couple of decades, advances in genomic sequencing technology have caused an unprecedented increase in biological data. Because of the quantity and size of these datasets, the need for biologists with computational expertise also increased, and using these large genomic datasets has revolutionized the way we can answer biological questions. In this dissertation, I apply genomic and transcriptomic data to answer questions about insects of the order Hemiptera that are relevant to agriculture and public health. My dissertation mainly focuses on stink bugs (Hemiptera: Pentatomidae), many of which are agricultural pests, and bed bugs (Hemiptera: Cimicidae), which are …

Development Of Novel Nanocarriers With Multi Modal Action Against Lung Cancer, Gayathri K

Theses and Dissertations

Lung cancer ranks among the top causes of cancer-related deaths worldwide. The deteriorating environment and unhealthy lifestyle in the modern era has further contributed to the exponential increase in lung cancer incidence across the globe. Lung cancers fall under two major types based on the location of the tumour in the lung namely, small-cell lung cancer (SCLC) which is more aggressive, and non-small-cell lung cancer (NSCLC) which is more prevalent. Late diagnosis has been a major cause for poor prognosis as the cancer is detected only in an advanced stage.

Conventional therapies like chemotherapy, surgery and radiotherapy against lung cancer …

Oregon State Rank Assessment For Mardon Skipper (Polites Mardon), Eleanor P. Gaines

Institute for Natural Resources Publications

Oregon state conservation status assessment for Mardon skipper (Polites mardon) using NatureServe methodology, 2024.

A Kid's Guide To Infectious Disease, Abby Carroll

Honors Theses

A Kid’s Guide to Infectious Disease is an educational illustrated book intended for young pediatric patients, ages three to five. This project was inspired by my personal interests, involvement at the University of Mississippi, and experience as a medical assistant. Over the past couple of years, I have had the opportunity to work as a medical assistant, interacting with many pediatric patients and their families at HealthPointe Primary Care. As a pre-physician assistant student studying biology, I was inspired to create a resource for these kids by both my classes at the University of Mississippi and my passion for pediatric …

Unmanned Aerial Systems (Uas) Image Preprocessing To Reduce Artifacts And Improve Geometric Registration When Generating Orthophoto Mosaics And 3d Models, Eddie Ironsmith

Electronic Theses and Dissertations

Drones can now be used to quickly collect imagery data in a highly automated way; however, individual images must be combined to form an orthomosaic or 3-Dimentional (3D) model using photogrammetry software. Currently, the existing software may generate erroneous output in the form of artifacts or positional errors caused by homogeneous areas, light reflections, object movement between photos, or sub-optimal algorithms. The goal of this research was to develop preprocessing algorithms that would filter movement (or other time or position-based differences) and areas of homogeneity. The hypothesis is that filtering these parts of the image would reduce artifacts and improve …

Innovative Methyl Bromide Alternatives: Efficacy Of Acetic Acid Coated Ham Nets And Carboxymethyl Cellulose Films In Inhibiting Mite Infestations On Dry-Cured Ham, Sawyer Wyatt Smith

Theses and Dissertations

Dry-cured hams may become infested with Tyrophagus putrescentiae (ham mites) during the aging process. Traditionally, methyl bromide (MB) fumigation has been used to control these infestations, but its phase-out due to environmental concerns has prompted the need for alternative methods. This study evaluated the effectiveness of acetic acid-coated ham nets and carboxymethyl cellulose (CMC)-based edible films with propylene glycol (PG) in mitigating mite infestations. Laboratory trials assessed the impact of these treatments on mite orientation, reproduction, infestation rates, and sensory quality. Results indicate that CMC films were effective at controlling mite growth and reproduction with minimal sensory impact. In contrast, …

Identification And Characterization Of The Antimicrobial Activities Of Pseudomonas Sp. Strain Wp18, Emma Suzanne Willis

Theses and Dissertations

Strain WP18 was isolated from disease suppressive soil of a soybean rhizosphere in the Mississippi Delta. Phylogenetic analysis supported that strain WP18 could represent a novel species within the genus Pseudomonas. This conclusion is supported by genome-wide bioinformatic analyses such as average nucleotide identity and genome to genome distance calculations. Further studies are needed to claim a novel bacterial species, which include cell fatty profiles, fingerprinting analysis, and polar lipid analysis based on the International Code for Nomenclature of Prokaryotes. Strain WP18 showed a broad-spectrum of antimicrobial activity against plant pathogenic bacteria and fungi that are economically important in agriculture, …

Identification Of Bacterial Species Composition And Diversity Of Chicken Breast Meat During Processing., Lizzie Raquel Zaldivar

Theses and Dissertations

Understanding the bacterial profile of chicken during processing is essential to identify spoilage-related bacteria. Using advanced methods like Oxford Nanopore sequencing, specific bacterial species on chicken meat can be identified. This study aimed to characterize bacterial composition and diversity on chicken breast during processing. Results showed a reduction in aerobic bacteria, E. coli, coliform, and lactic acid bacteria counts. However, three thermophilic bacteria, Anoxybacillus contaminants, Neobacillus thermocopriae, and Anoxybacillus flavithermus, dominated across processing steps. Notably, Pseudomonas species were undetected by conventional plating but identified via Nanopore sequencing, highlighting the need for molecular detection methods. Although antimicrobial interventions significantly reduced bacterial …

Development And Changes Over Time Of A Granola Bar Formulated With Sweet Potatoes, Meredith Kate Maynard

Theses and Dissertations

Sweet potatoes are a worldwide staple crop and granola bars are popular with consumers. This study aims to create a granola bar utilizing sweet potatoes and to assess its changes over time. Initial formulations tested minimum and maximum concentration of sweet potato (SP) puree, formulations, cooking type and time/temperature, humectants and preservatives. Bars containing 30 and 45% SP butter were chosen. Changes over time and descriptive sensory evaluation were conducted every 14d for 70d, with four treatments: a control bar and a bar with a preservative, each held at 25°C and 47°C. Water activity and moisture decreased by 0.05 and …

Effects Of A Snap-Ed Social Marketing Campaign On Behavior Change, Andrew Beall Cole

Theses and Dissertations

Introduction: This research studied the use of a social marketing campaign to address chronic diseases in the U.S., such as obesity, diabetes, hypertension and other cardiovascular diseases by assessing audience self-reported actions and evaluating message framing effects on participant intentions to change nutrition behavior and daily physical activity. Approach: Evaluate a social marketing campaign for effects on SNAP-Ed eligible clients and research message framing effects on audience’s intention to change health behaviors. Discussion: The HappyHealthy social marketing campaign launched in 2018 by Mississippi State University Extension. Outcome evaluation data (collected 2023) reported multiple correlations between core messages recalled and increased …

Evaluation Of Current Management Strategies For The Tarnished Plant Bug And Tobacco Thrips In Midsouthern Cotton, Hunter Lane Lipsey

Theses and Dissertations

The tarnished plant bug, Lygus lineolaris (Palisot de Beauvois), is an important economic pest of cotton, Gossypium hirsutum (L.), in the Midsouth region of the United States. An experiment was conducted to determine the impact of insecticide class loss on yield and Lygus populations in the recently commercialized ThryvOn cotton technology. Data indicated no significant yield loss when excluding any currently utilized insecticide class for tarnished plant bug management. An additional objective was to reevaluate the sampling interval necessary to scout ThryvOn cotton for Lygus. No significant differences were observed between a 3 or 7-day sample interval compared to plots …

Effects Of Eugenol And Carvacrol In Combination Against Salmonella In Hummus, Lauryn Ashley Heidelberg

Theses and Dissertations

Hummus consumption has risen but multiple foodborne outbreaks due to Salmonella contamination have been reported. Essential oils (EOs) eugenol and carvacrol have been found to inhibit the growth and survival of Salmonella in food products. The objective was to evaluate antibacterial properties of various concentrations of eugenol (EU) and carvacrol (CA), alone or in combination (EUCA) against Salmonella in vitro and in hummus. Eugenol demonstrated a MIC of 0.125 mg/mL and an MBC of 0.25 mg/mL, while carvacrol showed a MIC of 0.0625 mg/mL and an MBC of 0.125 mg/mL. In the hummus study, 2% EUCA was the most effective …

Evaluation Of Soybean (Glycine Max L.) Seed Inoculation With Bradyrhizobium Japonicum And Bacillus Subtilis On Yield And Root System Architecture: A Herbicide Carryover Perspective, Ncomiwe Andile Maphalala

Theses and Dissertations

Reducing herbicide carryover in soybean production is imperative to sustain future soybean cropping systems in Mississippi and the rest of the world. This research was conducted to determine the response of inoculated soybean to corn (Zea mays L.) residual herbicides. The herbicide treatments included aatrex (atrazine), callisto (mesotrione), lexar (atrazine+mesotrione+s-metolachlor), and steadfast (nicosulfuron+rimsulfuron) at 100%, 75%, 50%, and 25% of the total label rate. Inoculant treatments were Bradyrhizobium japonicum, Bacillus subtilis, and no inoculant. Bacterial inoculants positively impacted most shoot and root growth parameters, significantly increasing depending on the herbicide x inoculant treatment combination and its rate. B.japonicum alone and …

A Correlation Study Between Soil Selenium Levels And Diabetes Prevalence Across U.S. Regions: An Archival Data Analysis (2012-2018), Baoshan Wu

Theses and Dissertations

This study investigates the correlation between soil selenium levels and Type 2 diabetes (T2D) prevalence across U.S. regions between 2012 to 2018. Selenium, an essential trace element, plays an important role in antioxidant defense and metabolism. While appropriate selenium intake may protect against diabetes by improving insulin sensitivity and reducing oxidative stress, excessive intake can have adverse effects. This research analyzed the most recent data, from the Centers for Disease Control and Prevention (CDC) and the U.S. Geological Survey (USGS), for over 3,000 counties in the U.S. A correlation analysis model was used to determine the relationship between soil selenium …

Identifying Opportunities For Nature-Based Solutions With Geospatialized Life Cycle Assessments And Fine-Scale Socioecological Data, Gabriela Shirkey, Annick Anctil, Ranjeet John, Venkatesh Kolluru, Leah Mungai, Herve Kashongwe, Lauren T. Cooper, Ilke Celik, Joshua B. Fisher, Jiquan Chen

Biology, Chemistry, and Environmental Sciences Faculty Articles and Research

As we increasingly understand the impact that land management intensification has on local and global climate, the call for nature-based solutions (NbS) in agroecosystems has expanded. Moreover, the pressing need to determine when and where NbS should be used raises challenges to socioecological data integration as we overcome spatiotemporal resolutions. Natural and working lands is an effort promoting NbS, particularly emissions reduction and carbon stock maintenance in forests. To overcome the spatiotemporal limitation, we integrated life cycle assessments (LCA), an ecological carbon stock model, and a land cover land use change model to synthesize rates of global warming potential (GWP) …

Key Epigenetic And Signaling Factors In The Formation And Maintenance Of The Blood-Brain Barrier, Jayanarayanan Sadanandan, Sithara Thomas, Iny Elizabeth Mathew, Zhen Huang, Spiros L Blackburn, Nitin Tandon, Hrishikesh Lokhande, Pierre D Mccrea, Emery H Bresnick, Pramod K Dash, Devin W Mcbride, Arif Harmanci, Lalit K Ahirwar, Dania Jose, Ari C Dienel, Hussein A Zeineddine, Sungha Hong, Peeyush Kumar T

Faculty, Staff and Student Publications

The blood-brain barrier (BBB) controls the movement of molecules into and out of the central nervous system (CNS). Since a functional BBB forms by mouse embryonic day E15.5, we reasoned that gene cohorts expressed in CNS endothelial cells (EC) at E13.5 contribute to BBB formation. In contrast, adult gene signatures reflect BBB maintenance mechanisms. Supporting this hypothesis, transcriptomic analysis revealed distinct cohorts of EC genes involved in BBB formation and maintenance. Here, we demonstrate that epigenetic regulator's histone deacetylase 2 (HDAC2) and polycomb repressive complex 2 (PRC2) control EC gene expression for BBB development and prevent Wnt/β-catenin (Wnt) target genes …

A Kinetic Model For Compound Heterozygous Pathogenic Variants In Tyrosyl-Trna Synthetase Gene Yars2-Associated Neonatal Phenotype, Thomas W Christian, Sunita Maharjan, Sitao Yin, Yuka Yamaki, Isao Masuda, Fenglin Li, Colleen Muraresku, Sheila Clever, Rebecca Ganetzky, Ya-Ming Hou

Department of Biochemistry and Molecular Biology Faculty Papers

Human genetic disorders are often caused by mutations of compound heterozygosity, where each allele of the mutant gene harbors a different genetic lesion. However, studies of such mutations are hampered due to the lack of an appropriate model. Here we describe a kinetic model of compound heterozygous variants in an obligate enzyme dimer that contains one mutation in one monomer and the other mutation in the second monomer. This enzyme is encoded by human YARS2 for mitochondrial tyrosyl-tRNA synthetase (mt-TyrRS), which aminoacylates tyrosine to mt-tRNA^Tyr. YARS2 is a member of the genes for mt-aminoacyl-tRNA synthetases, where pathogenic mutations …

Ecological Niches Applied To Socioeconomic Phenomena: The Case Of The Colombian Internal Conflict, Alexander Alegría, Laura Jiménez, Jhon Jairo Alvarado-Quintero

Peace and Conflict Studies

Recent studies of the Colombian conflict have shown that struggle for resource accumulation and territorial control, in a very competitive and violent environment in which the illegal armed groups are involved, is crucial to their survival and development. Here, we applied a highly successful methodology from ecology (Ecological Niche Modelling) to the problem of identifying the ideal socioeconomic conditions for the presence of a Colombian illegal armed group, named the Revolutionary Armed Forces of Colombia (FARC). We analyzed some determining factors of FARC´s presence and permanence within the Colombian territory that reflect aspects of the socioeconomic dynamics and productive capacity …

Wildlife Ambassadors, Carissa Lukac, Ibrahim Bhutta, Sunishka Deshpande, Amogh Jani

Purdue Journal of Service-Learning and International Engagement

Students at Purdue University are largely unaware of conservation efforts and research being done by both their fellow students and local organizations. This is why under the guidance of our mentor and as a part of the HIFI Lab, our group, the Wildlife Ambassadors, has created a website meant to push ecological efforts at Purdue University. This is done through the use of animal ambassadors, including but not limited to a wolf at Wolf Park, a local organization with which this project has been conducted in correspondence. This website will integrate and display the work of over three dozen other …

Jovial Family Farm Facilitates Intergenerational Learning Opportunities Through Urban Farming, Maera Williams

Purdue Journal of Service-Learning and International Engagement

Maera Williams is a sophomore in Environmental and Ecological Engineering at Purdue University. She joined the Engineering Projects in Community Service (EPICS) Urban Farming team as a freshman and now serves as project manager of ten undergraduate students collaborating with Jovial Family Farm to design and develop three indoor hydroponics systems for a laboratory space on their property in Broad Ripple, IN. Jovial Family Farm is a nonprofit organization founded by Drs. Bobbie and Brian Jellison with the mission to provide senior citizens, their families, and their communities with meaningful engagement opportunities on an intergenerational scale through urban agricultural activities. …

Wolf Park, Pawsing For Impactful Beginnings: The Role Of Community Engagement And Education In Service-Learning, John W. Robbins, Deniz Eksioglu, Kaushal Patel

Purdue Journal of Service-Learning and International Engagement

Our team’s endeavor, conducted under the guidance of Dr. Kristen Bellisario, encapsulates a service-learning journey with Wolf Park, a sanctuary dedicated to the conservation and research of wolves and wildlife. Through immersive experiences, community outreach, and collaborative partnerships, our team sought to amplify public understanding of wildlife conservation and inspire a sense of responsibility among participants. This essay examines the impact of our initiatives on both the community and ourselves, emphasizing the transformative potential of education, engagement, and community partnerships in effecting positive societal change. Our team advocates for sustained support for wildlife conservation efforts and underscore the necessity of …

Beyond The Bench: Redefining Research Impact In Society, Nouran Amin

Purdue Journal of Service-Learning and International Engagement

The following essay reports on the reflective experiences of the author’s time completing a certificate program “Advancing Graduate Research Impact in Society” offered by Purdue University’s Office of Community Engagement in Fall 2023. It begins by narrating the author's journey as a Muslim woman in STEM spaces, highlighting the lack of relatable role models and the ensuing dissonance between aspirations and prevailing narratives. Through introspection, the essay progresses to examine the paradox of publicly funded but inaccessible science, which often fails to engage with community needs and priorities. The essay underscores the importance of inclusivity and diverse representation in scientific …

Michigan Academician, Volume 49, Issue 1

Michigan Academician

2024 Conference Abstracts

5-Substituted Flavones—Another Class Of Potent Triplex Dna-Specific Ligands As Antigene Enhancers, Landy Gu, Nghia Tran, Vanessa M. Rangel, Mandeep Singh, Krege M. Christison, Geoff P. Lin-Cereghino, Liang Xue

Pacific Faculty Work

In the field of drug development, the quest for novel compounds that bind to DNA with high affinity and specificity never ends. In the present work, we report the newest development in this field, namely, triplex DNA-specific binding ligands based on the 5-substituted flavone scaffold in our lab. Biophysical studies showed that the newly synthesized flavone derivatives (depending on the side chains) bind to triplex DNA with binding affinities better than or similar to 5-substituted 3,3′,4′,7-tetramethoxyflavonoids. These compounds selectively stabilize triplex DNA while having little effect on duplex DNA, as verified by various biophysical methods. A detailed structural analysis suggested …