Life Sciences Commons^™

Keep Working Hands On Working Land, Elyse Caiazzo

Graduate Student Portfolios, Professional Papers, and Capstone Projects

Strategies for Effective Farmland Transitions, Succession Planning, and Landowner Education

Investigating The Effectiveness Of Microplastic Removal And The Relationship Between Microplastics, Bacterial Communities, And Nitrogen Cycling In The Natural Treatment Systems At The Arcata Wastewater Treatment Facility, Fiona Connor

Cal Poly Humboldt theses and projects

This thesis addressed two challenging aspects of microplastic research, including the quantification of microplastics in organic-rich water, and the investigation of how microplastics impact microorganisms and related nutrient-cycling. Two projects were designed and executed to address specific research objectives relating to these broad topics. The first objective was to develop and implement methods to determine the effectiveness of microplastic removal across the natural treatment systems at the Arcata Wastewater Treatment Facility (AWTF). The second objective was to observe how microplastics found in domestic wastewater impact the bacterial communities and nitrogen cycling in freshwater treatment wetland sediment through a bench-scale experiment, …

Effect Of In Situ Experimental Heating Of Artificial Mytilus Californianus Beds On Associated Invertebrate Communities In Northern California, Claire C. Windecker

Cal Poly Humboldt theses and projects

With the continued progression of climate change, the role of foundation species as mediators of temperature stress for species living on and within them has become increasingly important. In the intertidal zone, a major foundation species is the California mussel, Mytilus californianus, which can help to limit thermal stress for species that live among aggregations of these mussels. The ability of mussels to limit thermal stress as temperatures increase, however, is still unclear. This study sought to experimentally manipulate surface temperatures of artificial mussel beds in situ to examine the effects that increased surface heating at a single site …

Neural Crest Cell Migration And Developmental Origin Of Bony Scutes In White Sturgeon (Acipenser Transmontanus), Melody Tew

Cal Poly Humboldt theses and projects

Neural crest cells (NCCs) and mesodermal somites independently produce embryonic mesenchyme as a precursor to the chondrocytes and osteocytes of the vertebrate skeleton. In mammals and teleosts, NCCs only contribute to the dermal cranial skeleton, while the mesodermal somites and lateral plate mesoderm form the entire dermal and endochondral postcranial skeleton. In sharks, NCCs contribute to both the cranial and trunk integumentary skeleton. This suggests that contribution of the trunk neural crest mesenchyme to the integumentary skeleton was an ancestral condition for all vertebrates that was lost over evolutionary time in the osteichthyan lineage, but the evolutionary timing of this …

Comparing Mexican Spotted Owl Habitat Suitability In Two Different Habitat Types Using A Multi-Scale Ensemble Learning Framework, Danial Nayeri

Cal Poly Humboldt theses and projects

Habitat fragmentation and loss are major threats to species conservation worldwide. Studying species-habitat relationships is a crucial first step toward understanding species habitat requirements, which is necessary for conservation and management planning. However, some species inhabit a range of habitat types, potentially making the use of range-wide habitat models inappropriate due to non-stationarity. The Mexican spotted owl (Strix occidentalis lucida) (MSO) is a species that inhabits both forests and rocky canyonlands, two habitats with large differences in environmental conditions. It is unclear whether the species uses habitat differently in these two habitat types or if previously builthabitat models …

Investigating Tribal Co-Management Of Caifornia’S Public Lands, Zachary Joseph Erickson

Cal Poly Humboldt theses and projects

Collaborative management with Indigenous groups is becoming increasingly common as many Indigenous communities continue to assert their inherent rights to self-determination. Due to the removal from and dispossession of lands, tribes often rely on access to public properties for various uses including ceremonies and gathering of culturally important plants. Some believe that the absence of indigenous involvement has also led to a decline in both the quality and abundance of culturally important resources, as well as limited the intergenerational transfer of traditional ecological knowledge, or TEK. There is increasing momentum toward re-engaging tribes as stewards of their ancestral lands through …

Negative Social Media And Its Influence On Athlete's Performance, Bernd R. Huber

Cal Poly Humboldt theses and projects

This study aimed to investigate the potential impact of negative social media content on athletes' cortisol levels and subsequent performance. The study focused on the change in cortisol levels and differences in free throw performance, based on previous research findings. We hypothesized that negative social media postings would increase the stress experienced by student-athletes, resulting in elevated cortisol levels and decreased performance. Additionally, participants (n = 8) completed a questionnaire to examine the interaction between preexisting fear and the biological stress response. Contrary to expectations, there was no significant change in stress response, and negative postings did not have …

Sympatric Carnivores And Vegetation Structure Influence The Distribution And Abundance Of Humboldt Martens In Northern California, Erika L. Anderson

Cal Poly Humboldt theses and projects

The Humboldt marten (Martes caurina humboldtensis) is a species facing conservation concern in Oregon and California. Historical populations were subjected to unregulated fur trapping and timber harvest throughout the late 19^th and early 20^th centuries leading to a 90% decline in their distribution. As such, the Humboldt marten is listed as a state endangered species in California, and the analogous ‘coastal marten’ is listed as federally threatened under the Endangered Species Act and classified as a Species of Greatest Conservation Need in Oregon and California. Contemporary populations are small, and isolated, and threatened by habitat loss …

A Return To The Staging Grounds: Reassessment Of Aleutian Cackling Goose Spring Distribution In North Humboldt Bay, Shaun W. Thornton

Cal Poly Humboldt theses and projects

After a 15-year interval in research, spatial and temporal patterns of the Aleutian cackling goose during spring staging was assessed again in 2022. During my study, 3,389 goose flocks were recorded in Arcata Bottoms staging site, 1 January to 21 April 2022, amounting to an estimated total of 2,248,512 goosedays, which was 336.8% higher than 2007 estimates of 667,485 goosedays. Geese continued to use pastures with a wide range of landscape characteristics primarily represented by livestock grazing practices. Goose use was greater than availability on beef-cattle fields early in the season and dairy-cow fields later in the season. Human disturbances …

Rapidly Changing Range Limits In A Warming World: Critical Data Limitations And Knowledge Gaps For Advancing Understanding Of Mangrove Range Dynamics In The Southeastern Usa, Rémi Bardou, Michael J. Osland, Steven Scyphers, Christine Shepard, Karen E. Aerni, Jahson B. Alemu I, Robert Crimian, Richard H. Day, Nicholas M. Enwright, Laura C. Feher, Sarah L. Gibbs, Kiera O'Donnell, Savannah H. Swinea, Kalaina Thorne, Sarit Truskey, Anna R. Armitage, Ronald Baker, Josh L. Breithaupt, Kyle C. Cavanaugh, Erik S. Yando, A. Randall Hughes, Et Al.

Biological Sciences Faculty Publications

Climate change is altering species’ range limits and transforming ecosystems. For example, warming temperatures are leading to the range expansion of tropical, cold-sensitive species at the expense of their cold-tolerant counterparts. In some temperate and subtropical coastal wetlands, warming winters are enabling mangrove forest encroachment into salt marsh, which is a major regime shift that has significant ecological and societal ramifications. Here, we synthesized existing data and expert knowledge to assess the distribution of mangroves near rapidly changing range limits in the southeastern USA. We used expert elicitation to identify data limitations and highlight knowledge gaps for advancing understanding of …

Global Phylogenomic Assessment Of Leptoseris And Agaricia Reveals Substantial Undescibed Diversity At Mesophotic Depths, J. C. Gijsbers, N. Englebert, K. E. Prata, M. Pichon, Z. Dinesen, R. Brunner, G. Eyal, F. L. González-Zapata, S. E. Kahng, K. R. W. Latijnhouwers, P. Muir, V. Z. Radice, J. A. Sánchez, M. J. A. Vermeij, O. Hoegh-Guldberg, S. J. Jacobs, P. Bongaerts

Biological Sciences Faculty Publications

Background: Mesophotic coral communities are increasingly gaining attention for the unique biological diversity they host, exemplified by the numerous mesophotic fish species that continue to be discovered. In contrast, many of the photosynthetic scleractinian corals observed at mesophotic depths are assumed to be depth-generalists, with very few species characterised as mesophotic-specialists. This presumed lack of a specialised community remains largely untested, as phylogenetic studies on corals have rarely included mesophotic samples and have long suffered from resolution issues associated with traditional sequence markers.

Results: Here, we used reduced-representation genome sequencing to conduct a phylogenomic assessment of the two dominant mesophotic …

Corrigendum: Characterization Of A Novel Transitional Group Rickettsia Species (Rickettsia Tillamookensis Sp. Nov.) From The Western Black-Legged Tick, Ixodes Pacificus, David T. Gauthier, Sandor E. Karpathy, Stephanie L. Grizzard, Dhwani Batra, Lori A. Rowe, Christopher D. Paddock

Biological Sciences Faculty Publications

No abstract provided.

The Coral Bleaching Automated Stress System (Cbass): A Low Cost, Portable System For Standardized Empirical Assessments Of Coral Thermal Limits, Nicolas R. Evensen, Katherine E. Parker, Thomas A. Oliver, Stephen R. Palumbi, Cheryl A. Logan, James S. Ryan, Courtney N. Klepac, Gabriela Perna, Mark E. Warner, Christian R. Voolstra, Daniel J. Barshis

Biological Sciences Faculty Publications

Ocean warming is increasingly affecting marine ecosystems across the globe. Reef-building corals are particularly affected by warming, with mass bleaching events increasing in frequency and leading to widespread coral mortality. Yet, some corals can resist or recover from bleaching better than others. Such variability in thermal resilience could be critical to reef persistence; however, the scientific community lacks standardized diagnostic approaches to rapidly and comparatively assess coral thermal vulnerability prior to bleaching events. We present the Coral Bleaching Automated Stress System (CBASS) as a low-cost, open-source, field-portable experimental system for rapid empirical assessment of coral thermal thresholds using standardized temperature …

Territory Inheritance And The Evolution Of Cooperative Breeding In The Acorn Woodpecker, Walter D. Koenig, Joseph Haydock, Hannah L. Dugdale, Eric L. Walters

Biological Sciences Faculty Publications

There are two main hypotheses for why offspring in cooperatively breeding taxa delay dispersal and remain on their natal territory rather than disperse. First, ecological constraints may force offspring to remain on their natal territory until a reproductive opportunity presents itself in an otherwise saturated habitat. Alternatively, delaying dispersal and helping kin may increase an offspring's inclusive fitness. One means by which offspring might enhance their direct fitness by delaying dispersal is by inheriting breeding status on their natal territory. Such territory inheritance regularly occurs in acorn woodpeckers, Melanerpes formicivorus, a species whose social groups consist of a cooperatively …

Variation In Establishment Success For American Mistletoe [Phoradendron Leucarpum (Raf.) Reveal & M.C. Johnst. (Viscaceae)] Appears Most Likely To Predict Its Distribution In Virginia And North Carolina, United States, Nicholas P. Flanders, Christopher P. Randle, Eric L. Walters, Lytton J. Musselman

Biological Sciences Faculty Publications

Dispersal limitation and variation in habitat suitability may determine an association of American mistletoe [Phoradendron leucarpum (Raf.) Reveal & M.C. Johnst. (Viscaceae)] with forested wetlands in Virginia and North Carolina, United States. Here, we first tested the alternative hypothesis that variation in host availability drives this habitat relationship. We used a generalized linear model to show a positive effect of forested wetland habitat on American mistletoe occurrence after accounting for both variation in host availability and differences among regions in host use. We then used seed sowing experiments to quantify how light availability and flood regime determine the viability …

Fine-Scale Genetic Structure In Rhizosphere Microbial Communities Associated With Chamaecrista Fasciculata (Fabaceae), Mahboubeh Hosseinalizadeh Nobarinezhad, Lisa E. Wallace

Biological Sciences Faculty Publications

Soil microbiota of the rhizosphere are an important extension of the plant phenotype because they impact the health and fitness of host plants. The composition of these communities is expected to differ among host plants due to influence by host genotype. Given that many plant populations exhibit fine-scale genetic structure (SGS), associated microbial communities may also exhibit SGS. In this study, we tested this hypothesis using Chamaecrista fasciculata, a legume species that has previously been determined to have significant SGS. We collected genetic data from prokaryotic and fungal rhizosphere communities in association with 70 plants in an area of …

Co-Occurrence Of Marine And Freshwater Phycotoxins In Oysters, And Analysis Of Possible Predictors For Management, Sarah K. D. Pease, Todd A. Egerton, Kimberly S. Reece, Marta P. Sanderson, Michelle D. Onofrio, Evan Yeargan, Adam Wood, Amanda Roach, I-Shuo Wade Huang, Gail P. Scott, Allen R. Place, Amy M. Hayes, Juliette L. Smith

Biological Sciences Faculty Publications

Oysters (Crassostrea virginica) were screened for 12 phycotoxins over two years in nearshore waters to collect baseline phycotoxin data and to determine prevalence of phycotoxin co-occurrence in the commercially and ecologically-relevant species. Trace to low concentrations of azaspiracid-1 and -2 (AZA1, AZA2), domoic acid (DA), okadaic acid (OA), and dinophysistoxin-1 (DTX1) were detected, orders of magnitude below seafood safety action levels. Microcystins (MCs), MC-RR and MC-YR, were also found in oysters (maximum: 7.12 μg MC-RR/kg shellfish meat wet weight), warranting consideration of developing action levels for freshwater phycotoxins in marine shellfish. Oysters contained phycotoxins that impair shellfish health: karlotoxin1-1 and …

Mechanical Devices For Census And Detection Of Off-Host Larval Ticks (Acari: Ixodidae), Donald B. Thomas, Holly D. Gaff, Brenda Leal-Galvan

Biological Sciences Faculty Publications

Standard off-host tick census techniques are generally inefficient for larval stages. Also, such techniques expose the investigator to environmental hazards including tick bites. Mechanical devices are an option which were tested under field conditions for proof of concept and in side-by-side comparison to the standard techniques. The tick-vac collected more ticks than the legging technique, but with lower frequency of positive detections. It may have utility for sampling small areas, such as weedy corrals suspected of harboring ticks. Different configurations of tick-bots, robotic vehicles with flags, depended on intensive operator control to avoid obstacles, limiting operation to line of sight. …

Blue Carbon Science, Management And Policy Across A Tropical Urban Landscape, Daniel A. Friess, Yasmine M. Gatt, Tze Kwan Fung, Jahson B. Alemu I, Natasha Bhatia, Rebecca Case, Siew Chin Chua, Danwei Huang, Valerie Kwan, Kiah Eng Lim, Yudhishthra Nathan, Yan Xiang Ow, Daniel Saavedra-Hortua, Taylor M. Sloey, Erik S. Yando, Hassan Ibrahim, Lian Pin Koh, Jun Yu Puah, Serena Lay-Ming Teo, Karenne Tun, Lynn Wei Wong, Siti Maryam Yaakub

Biological Sciences Faculty Publications

The ability of vegetated coastal ecosystems to sequester high rates of “blue” carbon over millennial time scales has attracted the interest of national and international policy makers as a tool for climate change mitigation. Whereas focus on blue carbon conservation has been mostly on threatened rural seascapes, there is scope to consider blue carbon dynamics along highly fragmented and developed urban coastlines. The tropical city state of Singapore is used as a case study of urban blue carbon knowledge generation, how blue carbon changes over time with urban development, and how such knowledge can be integrated into urban planning alongside …

A Multi-Isotope Approach Reveals Seasonal Variation In The Reliance On Marine Resources, Production Of Metabolic Water, And Ingestion Of Seawater By Two Species Of Coastal Passerine To Maintain Water Balance, Lucas Navarrete, Nico Lübcker, Felipe Alvarez, Roberto Nespolo, Juan Carlos Sanchez-Hernandez, Karin Maldonado, Zachary D. Sharp, John P. Whiteman, Seth D. Newsome, Pablo Sabat

Biological Sciences Faculty Publications

Tracing how free-ranging organisms interact with their environment to maintain water balance is a difficult topic to study for logistical and methodological reasons. We use a novel combination of triple-oxygen stable isotope analyses of water extracted from plasma (δ¹⁶O, δ¹⁷O, δ¹⁸O) and bulk tissue carbon (δ¹³C) and nitrogen (δ¹⁵N) isotopes of feathers and blood to estimate the proportional contribution of marine resources, seawater, and metabolic water used by two species of unique songbirds (genus Cinclodes) to maintain their water balance in a seasonal coastal environment. We …

The Practice And Promise Of Temporal Genomics For Measuring Evolutionary Responses To Global Change, René D. Clark, Katrina A. Catalano, Kyra S. Fitz, Eric Garcia, Kyle E. Jaynes, Brendan N. Reid, Allyson Sawkins, Anthony A. Snead, John C. Whalen, Malin L. Pinsky

Biological Sciences Faculty Publications

Understanding the evolutionary consequences of anthropogenic change is imperative for estimating long-term species resilience. While contemporary genomic data can provide us with important insights into recent demographic histories, investigating past change using present genomic data alone has limitations. In comparison, temporal genomics studies, defined herein as those that incorporate time series genomic data, utilize museum collections and repeated field sampling to directly examine evolutionary change. As temporal genomics is applied to more systems, species and questions, best practices can be helpful guides to make the most efficient use of limited resources. Here, we conduct a systematic literature review to synthesize …

Disparate Population And Holobiont Structure Of Pocilloporid Corals Across The Red Sea Gradient Demonstrate Species-Specific Evolutionary Trajectories, Carol Buitrago-López, Anny Cárdenas, Benjamin C.C. Hume, Thierry Gosselin, Fabian Staubach, Manuel Aranda, Daniel J. Barshis, Yvonne Sawall, Christian R. Voolstra

Biological Sciences Faculty Publications

Global habitat degradation heightens the need to better understand patterns of genetic connectivity and diversity of marine biota across geographical ranges to guide conservation efforts. Corals across the Red Sea are subject to pronounced environmental differences, but studies so far suggest that animal populations are largely connected, excepting evidence for a genetic break between the northern-central and southern regions. Here, we investigated population structure and holobiont assemblage of two common pocilloporid corals, Pocillopora verrucosa and Stylophora pistillata, across the Red Sea. We found little evidence for population differentiation in P. verrucosa, except for the southernmost site. Conversely, S. …

Floral And Genetic Divergence Across Environmental Gradients Is Moderated By Inter-Population Gene Flow In Platanthera Dilatata (Orchidaceae), Lisa E. Wallace, Marlin L. Bowles

Biological Sciences Faculty Publications

Understanding how natural selection acts on intraspecific variation to bring about phenotypic divergence is critical to understanding processes of evolutionary diversification. The orchid family is well known for pollinator-mediated selection of floral phenotypes operating among species and along environmental or geographic gradients. Its effectiveness at small spatial scales is less understood, making the geographic scale at which intraspecific floral variation is examined important to evaluating causes of phenotypic divergence. In this study, we quantified phenotypic variation in the orchid Platanthera dilatata across 26 populations in coastal Southeast Alaska and compared this to edaphic and genetic variation at microsatellite loci. We …

Are Epicuticular Waxes A Surface Defense Comparable To Trichomes? A Test Using Two Solanum Species And A Specialist Herbivore., Sakshi Watts, Rupesh R. Kariyat

School of Integrative Biological & Chemical Sciences (Formerly Dept. of Biology)

Although plants possess a suite of structural defenses, most studies have focused on trichomes. Trichomes can have both pre- and post-ingestive effects and have been consistently found to reduce herbivory. Along with trichomes, a few studies have focused on epicuticular waxes as an important defense; however, manipulated comparisons examining herbivore growth and development is limited. In this study, using two Solanum species (Solanum glaucescens and Solanum macrocarpon) that vary in both defenses, we tested the hypothesis that variation in defenses will affect herbivore feeding, primarily by restricting feeding commencement. We used electron microscopy together with a series of plant- and …

The Foxo1 Inhibitor As1842856 Triggers Apoptosis In Glioblastoma Multiforme And Basal-Like Breast Cancer Cells, David Flores, Alma Lopez, Shreya Udawant, Bonnie Gunn, Megan Keniry

School of Integrative Biological & Chemical Sciences (Formerly Dept. of Biology)

Basal-like breast cancer (BBC) and glioblastoma multiforme (GBM) are poor-prognosis cancers that lack effective targeted therapies and harbor embryonic stem gene expression signatures. Recently, our group and others found that forkhead box transcription factor FOXO1 promotes stem gene expression in BBC and GBM cell lines. Given the critical role of cancer stem cells in promoting cancer progression, we examined the impact of FOXO1 inhibition with AS1842856 (a cell-permeable small molecule that directly binds to unphosphorylated FOXO1 protein to block transcriptional regulation) on BBC and GBM cell viability. We treated a set of BBC and GBM cancer cell lines with increasing …

The Justice Challenge: Honors Endeavors Innovative Pedagogies Through The Grand Challenge Scholars Program, Jonathan Kotinek, Rebecca C. Bott-Knutson, Leigh E. Fine, Joy L. Hart, William Ziegler, Paul Knox, Timothy Nichols, Susan Sumner, Heidi Appel, Mark C. Andersen

School of Integrative Biological & Chemical Sciences (Formerly Dept. of Biology)

Honors practitioners from fourteen colleges and programs across the country at land-grant, public, and minority-serving institutions partner with agricultural experts to secure a United States Department of Agriculture (USDA) Higher Education Challenge grant focused on the themes of food justice, climate justice, and sustainable agriculture. Authors describe the program’s origin, objectives, and curricular outgrowths, highlighting its efficacy for empowering students in areas of systems thinking, career readiness, leadership in the service of addressing social needs, and learning through civic engagement.

Nilgai, Boselaphus Tragocamelus And White-Tailed Deer, Odocoileus Virginianus Use Of Water Troughs With And Without Remotely Operated Field Sprayers For Potential Treatment Of Cattle Fever Ticks, John A. Goolsby, Mauricio Cruz, Reyes Garcia Iii, Lauren P. Maestas, Teresa Patricia Feria-Arroyo

School of Integrative Biological & Chemical Sciences (Formerly Dept. of Biology)

Nilgai antelope, Boselaphus tragocamelus and white-tailed deer (WTD), Odocoileus virginianus are hosts of cattle fever ticks, Rhipicephalus (=Boophilus) microplus, in the Lower Rio Grande Valley of South Texas. Daily use of water troughs and ponds were observed to determine if these locations could be used for field treatment methods for cattle fever ticks using remotely operated field sprayers. Game cameras (n=16) were deployed between June 2020 and September 2022 at two private ranches in Cameron County, TX. Both nilgai and WTD visited water troughs equipped with motion detecting sprayers in similar numbers to ponds, but numbers of animal visits were …

Intra- And Inter-Rater Reliability Of Maximum Torque Applied During Passive Elbow Range Of Motion Testing Post-Casting Of Supracondylar And Lateral Condylar Fractures In Children, Joseph T. Armstrong

Theses and Dissertations--Kinesiology and Health Promotion

Elbow fractures account for a significant number of pediatric orthopedic injuries. The most common of these are the supracondylar and lateral condylar humeral fractures. When surgical repair is necessary casting and pinning of the elbow can affect the elbow’s range of motion (ROM), so recovery is monitored. Practices for measuring recovery are not standardized so measures may not be consistent. A possible novel measure to help account for different clinicians participating in the same study is maximum torque applied during elbow end ROM testing. This study uses several comparisons of maximum torque applied by three different clinicians during end ROM …

Effects Of Constant Load Exercise On Oxygen Consumption And Knee Joint Mechanics In Young Adults, Mariana Victoria Jacobs

Theses and Dissertations--Kinesiology and Health Promotion

The rate of obesity and its adverse health consequences have continued to rise in the past several decades. Aerobic constant load exercise is recommended to reduce excess body mass, however the effects of constant load exercise on oxygen consumption (VO2) and knee joint mechanics are inconsistent when varying weight-status strategies are employed. The purpose of this study was to assess the influence of different weight classification strategies on VO2 and knee joint mechanics during constant load exercise. Participants walked on an instrumented treadmill at a self-selected speed for 30-minutes while gait mechanics and VO2 measures were determined. Between-group differences were …

Responses To Exercise Anchored To Vigorous Intensity Heart Rates, Djadmann Gustave

Theses and Dissertations--Kinesiology and Health Promotion

Exercise prescription to improve cardiorespiratory endurance (CE) is often based on percentages of the reserve or maximum heart rate (HR_peak) or oxygen consumption rate (V̇O_2peak)that reflect vigorous intensities (77-95% HR_peak, 64-90% V̇O_2peak). These intensities elicit a rating of perceived exertion [RPE] response between 14-17 on the 6-20 Borg Scale. It is recommended that exercise within these ranges be sustained for 20-60min per session. The purposes of this study were to: 1) Determine the metabolic (V̇O₂) and perceptual (RPE) responses as well as the sustainability of exercise (time to exhaustion [T …