Life Sciences Commons^™

Machine Learning And Protein Allostery, Sian Xiao, Gennady M. Verkhivker, Peng Tao

Mathematics, Physics, and Computer Science Faculty Articles and Research

The fundamental biological importance and complexity of allosterically regulated proteins stem from their central role in signal transduction and cellular processes. Recently, machine-learning approaches have been developed and actively deployed to facilitate theoretical and experimental studies of protein dynamics and allosteric mechanisms. In this review, we survey recent developments in applications of machine-learning methods for studies of allosteric mechanisms, prediction of allosteric effects and allostery-related physicochemical properties, and allosteric protein engineering. We also review the applications of machine-learning strategies for characterization of allosteric mechanisms and drug design targeting SARS-CoV-2. Continuous development and task-specific adaptation of machine-learning methods for protein allosteric …

Author Correction: A Nanoengineered Topical Transmucosal Cisplatin Delivery System Induces Anti-Tumor Response In Animal Models And Patients With Oral Cancer, Manijeh Goldberg, Aaron Manzi, Amritpreet Birdi, Brandon Laporte, Peter Conway, Stefanie Cantin, Vasudha Mishra, Alka Singh, Alexander T Pearson, Eric R Goldberg, Sam Goldberger, Benjamin Flaum, Rifat Hasina, Nyall R London, Gary L Gallia, Chetan Bettegowda, Simon Young, Vlad Sandulache, James Melville, Jonathan Shun, Sonya E O'Neill, Erkin Aydin, Alex Zhavoronkov, Anxo Vidal, Atenea Soto, Maria Jose Alonso, Ari J Rosenberg, Mark W Lingen, Anil D'Cruz, Nishant Agrawal, Evgeny Izumchenko

Faculty, Staff and Student Publications

No abstract provided.

Doctoral Studies As Learning To Rename The World, Hyleen Mariaye

Journal of Multicultural Affairs

The reflective experience documented in this paper engages with doctoral learning from Freire’s (1968/2000) conceptual lens of naming the world. Written from the narrative lens of the supervisor, it considers how doctoral level studies in education can position both the supervisor and the candidates as agents actively reconstructing their understanding of the world and their place in it. The doctoral journey is viewed as praxis compelling researchers to expand their frames for reading the world, accommodating the other, including multiple voices and thus demonstrating commitment to a global and yet constantly contested notion of citizenship.

Poetry And Praxis: Lessons From An Activist Educator, Dr. Emmanuel Tabi

Journal of Multicultural Affairs

Drawing on data from a narrative multi-case study based in Toronto, Canada, this article discusses the lived experiences of one Black activist. Utilizing critical race theory, new literacy studies and the rhetoric of cultural production as theoretical frameworks, the article foregrounds the work of Ebele, a Toronto activist whose work supported the educational trajectories and emotional well-being of Black students, many of whom reported being marginalized in school. Through his creative labor, Ebele directly addressed the sociology of anti-Black racism that deeply influences the lives of Afrodiasporic people in Canada. This article continues the conversation about what it means to …

Large-Scale Crispri And Transcriptomics Of Staphylococcus Epidermidis Identify Genetic Factors Implicated In Lifestyle Versatility., Michelle Spoto, Johanna P. Riera Puma, Elizabeth Fleming, Changhui Guan, Yvette Ondouah Nzutchi, Dean Kim, Julia Oh

Faculty Research 2022

Staphylococcus epidermidis is a ubiquitous human commensal skin bacterium that is also one of the most prevalent nosocomial pathogens. The genetic factors underlying this remarkable lifestyle plasticity are incompletely understood, mainly due to the difficulties of genetic manipulation, precluding high-throughput functional profiling of this species. To probe the versatility of S. epidermidis to survive across a diversity of environmental conditions, we developed a large-scale CRISPR interference (CRISPRi) screen complemented by transcriptional profiling (RNA sequencing) across 24 diverse conditions and piloted a droplet-based CRISPRi approach to enhance throughput and sensitivity. We identified putative essential genes, importantly revealing amino acid metabolism as …

Establishment Of The Invasive Cactus Moth, Cactoblastis Cactorum (Berg) (Lepidoptera: Pyralidae) In Pakistan: A Potential Threat To Cultivated, Ornamental And Wild Opuntia Spp. (Cactaceae), Muhammad Ather Rafi, Harry Pavulaan, Muhammad Islam, Muhammad Ashfaq, Haseeb Kamran, Walija Fayaz, Gul Naz Parveen, Riffat Sultana, Ahmad Zia, Waqar Ahmed, Qudrat Ullah, Muhammad Qasim, Falak Naz, Nazeer Ahmed, Muhammad Tariq Khan, Muhammad Saeed, Jalal Hayat Khan

The Taxonomic Report of the International Lepidoptera Survey

Subsequent to the significant accomplishment of biological control of Opuntia weeds in Australia, the larvae of the cactus moth, Cactoblastis cactorum (native to parts of South America), were released in many countries for the biological control of native Opuntia species (Simmonds and Bennett, 1966). Inauspiciously, larvae were also released in the Caribbean, where the moth spread naturally and by the human support all over the region (García-Turudi et al., 1971). Its enhanced dissemination rate and the biological potential for invasiveness, suggests that the cactus moth is likely to become an invasive pest of Opuntia in the Southeast United States, Mexico, …

Effect Of Dietary Urea In Gestating Beef Cows: Circulating Metabolites, Morphometrics, And Mammary Secretions, Ligia D. Prezotto, Jennifer E. Thorson

Department of Animal Science: Faculty Publications

Prolific use of supplementation strategies, including the utilization of urea, are practiced in beef cattle production systems. Unfortunately, the influence of urea supplementation on metabolics, adipose tissue mobilization, and mammary secretions is limited in beef cows. Therefore, the objectives of this experiment were to assess the influence of urea supplementation on metabolic profiles, morphometrics, and mammary secretions. Pregnant, multiparous beef cows were fed individually and assigned to treatment (n = 4/treatment) as Control or Urea Supplementation. Blood samples and body weight were collected every 28 d throughout gestation. Backfat thickness was measured via ultrasonography on days 28 and 280 of …

Effect Of Dietary Urea In Gestating Beef Cows: Circulating Metabolites, Morphometrics, And Mammary Secretions, Ligia D. Prezotto, Jennifer F. Thorson

Department of Animal Science: Faculty Publications

Prolific use of supplementation strategies, including the utilization of urea, are practiced in beef cattle production systems. Unfortunately, the influence of urea supplementation on metabolics, adipose tissue mobilization, and mammary secretions is limited in beef cows. Therefore, the objectives of this experiment were to assess the influence of urea supplementation on metabolic profiles, morphometrics, and mammary secretions. Pregnant, multiparous beef cows were fed individually and assigned to treatment (n = 4/treatment) as Control or Urea Supplementation. Blood samples and body weight were collected every 28 d throughout gestation. Backfat thickness was measured via ultrasonography on days 28 and 280 of …

Surveillance Of Antimicrobial Resistance In Veterinary Medicine In The United States: Current E, Juliana M. Ruzante, Beth Harris, Paul Plummer, Raissa R. Raineri, John Dustin Loy, Megan Jacob, Orhan Sahin, Amanda J. Kreuder

Nebraska Center for Virology: Faculty Publications

Antimicrobial resistance (AMR) is a global problem facing human, animal, plant, and environmental health by threatening our ability to effectively treat bacterial infections with antimicrobials. In the United States, robust surveillance efforts exist to collect, analyze, and disseminate AMR data in human health care settings. These tools enable the development of effective infection control methods, the detection of trends, and provide the evidence needed to guide stewardship efforts to reduce the potential for emergence and further spread of AMR. However, in veterinary medicine, there are currently no known equivalent tools. This paper reviews efforts to reduce the potential for emergence …

Genetic Characterization Of Resitance To Frogeye Leaf Spot Of Soybean, Gabriel D. Munoz Herrera

LSU Master's Theses

Cercospora sojina is the causal agent of the fungal disease frogeye leaf spot (FLS) in soybeans. Management of this disease consists of foliar application of fungicides and the use of resistant cultivars. However, fungicide resistance has been detected in C. sojina populations and characterization of host resistance genes is relatively limited. To overcome the latter obstacle, we aimed to identify novel resistance genes using quantitative image analysis of the SoyNAM population. Two image analysis workflows using ImageJ and PlantCV were optimized and implemented to increase reproducibility and reduce subjectivity. The quantitative measurements included disease severity, lesion count, and average lesion …

Genetic Variation In Common Bunt Resistance In Synthetic Hexaploid Wheat, Amira M. I. Mourad, Alexey Morgounov, P. Stephen Baenziger, Samar M. Esmail

Department of Agronomy and Horticulture: Faculty Publications

Common bunt (caused by Tilletia caries and T. Foetida) is a major wheat disease. It occurs frequently in the USA and Turkey and damages grain yield and quality. Seed treatment with fungicides is an effective method to control this disease. However, using fungicides in organic and low-income fields is forbidden, and planting resistant cultivars are preferred. Due to the highly effective use of fungicides, little effort has been put into breeding resistant genotypes. In addition, the genetic diversity for this trait is low in modern wheat germplasm. Synthetic wheat genotypes were reported as an effective source to increase the …

Application Of Accuracy And Precision Evaluations Based On The Current United States And Indonesian Pharmacopoeias: A Critical Review, Gunawan Indrayanto

Makara Journal of Science

New methods for the evaluation of accuracy and precision are mentioned in the latest edition of the United States Pharmacopoeia (USP), whereas other validation parameters, that is, selectivity, linearity, range, and robustness, remained relatively unchanged. In obtaining reliable data from any chemical/pharmaceutical analysis, the analytical procedure must be validated or verified in accordance with the latest edition of the pharmacopoeia. Some review articles on the general validation methods have been published by the author. This present review will focus on the implementation and discussion of the accuracy and precision evaluation based on the current USP and Indonesian pharmacopoeia. Some examples …

Chitosan Oligosaccharides As A Nanomaterial Platform: Biological Properties And Applications In The Biomedical And Pharmaceutical Fields, Muhamad Alif Razi

Makara Journal of Science

Chitosan oligosaccharides (COS) have been introduced as marine-derived biomaterials with potential health benefits and good water solubility properties. This study presents an overview of the promising nanomaterial platform for biomedical and pharmaceutical applications of COS. The health benefits of COS, primarily their antioxidant and protective effects, anti-inflammatory activity, antidiabetic properties, and cholesterol-lowering effects are discussed. Furthermore, the promising recent articles on specific topics such as drug delivery systems and nanobiomaterials, are highlighted

Spatial Heterogeneity Of Infiltrating T Cells In High-Grade Serous Ovarian Cancer Revealed By Multi-Omics Analysis, Bin Yang, Xiong Li, Wei Zhang, Junpeng Fan, Yong Zhou, Wenting Li, Jingjing Yin, Xiaohang Yang, Ensong Guo, Xi Li, Yu Fu, Si Liu, Dianxing Hu, Xu Qin, Yingyu Dou, Rourou Xiao, Funian Lu, Zizhuo Wang, Tianyu Qin, Wei Wang, Qinghua Zhang, Shuaicheng Li, Ding Ma, Gordon B Mills, Gang Chen, Chaoyang Sun

Faculty, Staff and Student Publications

Tumor-infiltrating lymphocytes (TILs), especially CD8+ TILs, represent a favorable prognostic factor in high-grade serous ovarian cancer (HGSOC) and other tumor lineages. Here, we analyze the spatial heterogeneity of different TIL subtypes in HGSOC. We integrated RNA sequencing, whole-genome sequencing, bulk T cell receptor (TCR) sequencing, as well as single-cell RNA/TCR sequencing to investigate the characteristics and differential composition of TILs across different HGSOC sites. Two immune "cold" patterns in ovarian cancer are identified: (1) ovarian lesions with low infiltration of mainly dysfunctional T cells and immunosuppressive Treg cells and (2) omental lesions infiltrated with non-tumor-specific bystander cells. Exhausted CD8 T …

Surveillance Of Antimicrobial Resistance In Veterinary Medicine In The United States: Current Efforts, Challenges, And Opportunities, Juliana M. Ruzante, Beth Harris, Paul Plummer, Raissa R. Raineri, John Dustin Loy, Megan Jacob, Orhan Sahin, Amanda J. Kreuder

School of Veterinary and Biomedical Sciences: Faculty Publications

Antimicrobial resistance (AMR) is a global problem facing human, animal, plant, and environmental health by threatening our ability to effectively treat bacterial infections with antimicrobials. In the United States, robust surveillance efforts exist to collect, analyze, and disseminate AMR data in human health care settings. These tools enable the development of effective infection control methods, the detection of trends, and provide the evidence needed to guide stewardship efforts to reduce the potential for emergence and further spread of AMR. However, in veterinary medicine, there are currently no known equivalent tools. This paper reviews efforts to reduce the potential for emergence …

Does Receiving A Sars-Cov-2 Antibody Test Result Change Covid-19 Protective Behaviors? Testing Risk Compensation In Undergraduate Students With A Randomized Controlled Trial, Christina Ludema, Molly S Rosenberg, Jonathan T Macy, Sina Kianersi, Maya Luetke, Chen Chen, Lilian Golzarri-Arroyo, Erin Ables, Kevin Maki, David B Allison

Children’s Nutrition Research Center Staff Publications

Background: Risk compensation, or matching behavior to a perceived level of acceptable risk, can blunt the effectiveness of public health interventions. One area of possible risk compensation during the SARS-CoV-2 pandemic is antibody testing. While antibody tests are imperfect measures of immunity, results may influence risk perception and individual preventive actions. We conducted a randomized control trial to assess whether receiving antibody test results changed SARS-CoV-2 protective behaviors.

Purpose: Assess whether objective information about antibody status, particularly for those who are antibody negative and likely still susceptible to SARS-CoV-2 infection, increases protective behaviors. Secondarily, assess whether a positive antibody test …

Physical Activity Independently Predicts Perceived Stress During The Covid-19 Pandemic In Private University Students, Curtis W. Brownell, Laura S. Kabiri, Cassandra S. Diep, Heidi Y. Perkins, Amanda M. Perkins-Ball, Augusto X. Rodriguez

International Journal of Exercise Science

International Journal of Exercise Science 15(7): 1680-1691, 2022. Physical activity has significantly declined during the COVID-19 pandemic. Declines in physical activity have correlated with increased levels of perceived stress, though studies examining physical activity and stress have failed to account for critical confounds. The present study aims to determine whether physical activity independently predicts perceived stress in students attending private four-year universities. Physical activity, socioeconomic status, resilience, gender, and perceived stress data were collected from 85 students and used in a multiple linear regression analysis. The regression model accounted for 43.5% of the variance in perceived stress (R2 = .462, …

Muscle Architecture And Strength Changes Induced By Different Resistance Training Frequencies And Detraining, Tiago Volpi Braz, Felipe Brigatto, Danilo Rodrigues Batista, Wellington Gonçalves Dias, Luan Oenning Col, Guilherme Borsetti Businari, Jhenipher Rosolem, Julio Benvenutti Bueno De Camargo, Paulo Henrique Barbosa, Charles Ricardo Lopes

International Journal of Exercise Science

International Journal of Exercise Science 15(4): 1661-1679, 2022. The purpose of the present study was to investigate muscle thickness and strength outcomes of the quadriceps femoris induced by different resistance training (RT) frequencies and detraining. In addition, muscle architecture (MA) parameters were also assessed. Twenty-seven healthy resistance-trained subjects (men, n = 17; women, n = 10; 20.8 ± 1.9 years; RT experience = 3.3 ± 1.6 years) volunteered to participate in this study. One leg of each subject was randomly allocated into the 2 sessions per week condition (2x) and the contralateral leg was then placed in the 4 sessions …

Compatibility Of Crude Oil Blends─Processing Issues Related To Asphaltene Precipitation, Methods Of Instability Prediction─A Review, Krzysztof Bambinek, Andrzej Przyjazny, Grzegorz Boczkaj

Natural Sciences Publications

Processing crude oil of variable composition, especially due to the need to process crude oil blends obtained from various sources, presents a tremendous process challenge. This is mainly due to the destabilization of the colloidal system manifested mostly by the precipitation of the asphaltene fraction. The precipitation of asphaltenes from crude oil is a serious problem during extraction, transport, and processing. In the latter case, engineers and scientists have spent a lot of time determining what mechanisms are conducive to the occurrence of this phenomenon. On the one hand, there was a scientific curiosity about the principles of the nanoworld …

6k1, Nia-Vpg, Nia-Pro, And Cp Ofwheat Streak Mosaic Virus Are Collective Determinants Of Wheat Streak Mosaic Disease In Wheat, Satyanarayana Tatineni, Jeffrey Alexander, Haritha Nunna

Department of Plant Pathology: Faculty Publications

Wheat streak mosaic virus (WSMV; genus Tritimovirus, family Potyviridae) is the causal agent of the most economically important wheat streak mosaic disease of wheat (Triticum aestivum) in the Great Plains region of theUnited States.WSMVdeterminants responsible forwheat streak mosaic disease in wheat are unknown. Triticum mosaic virus (TriMV), a wheatinfecting virus,was used as an expression vector for the transient expression of each of the WSMV-encoded cistrons in wheat. WSMV-encoded 6K1, NIa-VPg, NIa-Pro, and CP cistrons in TriMV elicited symptoms specific to different stages of wheat streak mosaic disease without significantly affecting the genomic RNA accumulation. WSMV 6K1 …

Antibody Epitope Profiling Of The Kshv Lana Protein Using Virscan, Sydney J. Bennett, Dicle Yalcin, Sara R. Privatt, Owen Ngalamika, Salum J. Lidenge, John T. West, Charles Wood

School of Biological Sciences: Faculty Publications

The humoral antibody response against Kaposi sarcoma-associated herpesvirus (KSHV) in infected individuals has been characterized demonstrating the latency-associated nuclear antigen (LANA) as the most antigenic KSHV protein. Despite the antigenicity of the protein, specific LANA epitopes have not been systematically characterized. Here, we utilized a bacteriophage T7 library, which displays 56-amino acid KSHV LANA peptides with 28-amino acid overlap (VirScan), to define those epitopes in LANA targeted by antibodies from a cohort of 62 sub-Saharan African Kaposi sarcoma (KS) patients and 22 KSHV-infected asymptomatic controls. Intra- and inter-patient breadth and magnitude of the anti-LANA responses were quantified at the peptide …

Population Genomics Provide Insights Into The Global Genetic Structure Of Colletotrichum Graminicola, The Causal Agent Of Maize Anthracnose, Flávia Rogério, Riccardo Baroncelli, Francisco Borja Cuevas-Fernández, Sioly Becerra, Joanne Crouch, Wagner Bettiol, M. Andrea Azcárate-Peril, Martha Malapi-Wight, Veronique Ortega, Javier Betran, Albert Tenuta, José S. Dambolena, Paul D. Esker, Pedro Revilla, Tamra A. Jackson-Ziems, Jürg Hiltbrunner, Gary Munkvold, Ivica Buhiniček, José L. Vicente-Villardón, Serenella A. Sukno, Michael R. Thon

Department of Plant Pathology: Faculty Publications

Understanding the genetic diversity and mechanisms underlying genetic variation in pathogen populations is crucial to the development of effective control strategies. We investigated the genetic diversity and reproductive biology of Colletotrichum graminicola isolates which infect maize by sequencing the genomes of 108 isolates collected from 14 countries using restriction site-associated DNA sequencing (RAD-seq) and wholegenome sequencing (WGS). Clustering analyses based on single-nucleotide polymorphisms revealed three genetic groups delimited by continental origin, compatible with short-dispersal of the pathogen and geographic subdivision. Intra- and intercontinental migration was observed between Europe and South America, likely associated with the movement of contaminated germplasm. Low …

Challenges Experienced By Aquaponic Hobbyists, Producers, And Educators, D Allen Pattillo, David J. Cline, Janelle V. Hager, Luke A. Roy, Terrill R. Hanson

Journal of Extension

We used an online survey to document challenges experienced by aquaponic hobbyists (n = 81), producers (n = 117), and educators (n = 75). Responses were distilled into the following categories: 1) operations and management; 2) facilities, location, and system design; 3) knowledge and educational resources; 4) funding; 5) economic viability; 6) plant culture; 7) marketing and distribution; 8) fish culture; 9) human factors; 10) regulations and certifications. Training and research in these areas are needed to advance the aquaponics industry.

Estimated Impact Of X-Disease And Little Cherry Disease In Washington And Oregon From 2015 To 2020, Cody Molnar, Tianna Dupont, Ashley Thompson

Journal of Extension

X-disease phytoplasma and little cherry virus 1 and 2 have resulted in substantial loss of stone fruit acreage in Washington and Oregon. The extent and financial impact on the industry was not previously known. A Washington and Oregon State University survey documented 238,856 trees equivalent to 974 acres of sweet cherries removed due to X-disease and little cherry disease between 2015 and 2020. Removed trees reduced revenue to the industry by an estimated $30 million in 2020 and $65 million between 2015 and 2020. Over the seven-year re-establishment period estimated lost revenue and establishment costs to growers is an estimated …

Development And Assessment Of A Food Safety Training Program For Farmers’ Market Vendors, Joshua Scheinberg, Rama Radhakrishna, Catherine Cutter

Journal of Extension

Based on results collected through a comprehensive needs assessment of farmers market (FM) vendors in Pennsylvania, the purpose of this study was to develop and pilot-test a customized, food safety training program for FM vendors. A customized 3-hour, in-person, training program was developed and pilot tested. Using pre- and post-test assessment tests through piloted training, the results found participants scores on knowledge questions increased significantly by ~20% (p

Does The Anastatica Hierochuntica Plant Shorten The Duration Of Labour? A Prospective Observational Study, Filiz Okumuş, Halime Öncü-Çelik, Nevin Şahin

Journal of Asian Midwives (JAM)

Objective: This study aims to investigate the effect of Anastatica Hierochuntica L. on the duration of labour in primiparous women.
Methods: A prospective, case-controlled study with an intervention (n=28) and control (n=29) group. Participants were not randomized; their preferences related to the use of the A. Hierochuntica L. determined the group in which they would be included. In the intervention group, Anastatica Hierochuntica L. was used by all women. Standard care continued for both the intervention and control group. The duration of the first and second stage of labour were evaluated and compared between groups.
Results: Participants’ mean of age …

Colored Sweet Bell And Tapered Pepper Cultivar Evaluation For High Tunnel Production In West-Central Indiana, 2022, Petrus Langenhoven, Lian Mahorith Duron Alvarado, Eduardo Jose Miranda Oviedo

Midwest Vegetable Trial Reports

Colored sweet bell-shaped and tapered peppers are a staple at the farmers' market. Many small and medium-sized farming operations in Indiana grow this summer crop. High tunnels allow growers to protect their crops and extend their growing season. Sweet peppers, in particular, benefit from the unique growing environment created by the high tunnel. Planting of peppers can start at least 2-4 weeks earlier in the spring, and production can continue into the fall until the first hard freeze. Pepper variety performance data for Indiana is not readily available. We are working hard to change that. Currently, we have evaluated twenty-five …

Droughts Reduce Growth Rates And Increase Vulnerability To Increasingly Frequent And Severe Drying Events In An Aquatic Ectotherm, Thomas M. Luhring, Lyndsie S. Wszola, Grant M. Connette, Christopher M. Schalk

School of Biological Sciences: Faculty Publications

Many aquatic organisms are experiencing increasingly severe and frequent droughts and drying events. Simultaneously, drought effects are carrying over to non-drought years as ecosystems remain in incomplete states of recovery. Aquatic organisms are thus faced with fewer sequential years under degraded environmental conditions to prepare for increasingly severe droughts and potential drying events. We assessed the effect of droughts and sex on the growth, mass, and mass-dependent estivation potential of long-lived aquatic salamanders (Greater Sirens, Siren lacertina) that estivate during drying events brought on by severe droughts. We calculated growth rates of S. lacertina based on mark–recapture data spanning …

Droughts Reduce Growth Rates And Increase Vulnerability To Increasingly Frequent And Severe Drying Events In An Aquatic Ectotherm, Thomas M. Luhring, Lyndsie S. Wszola, Grant M. Connette, Christopher M. Schalk

School of Biological Sciences: Faculty Publications

Many aquatic organisms are experiencing increasingly severe and frequent droughts and drying events. Simultaneously, drought effects are carrying over to nondrought years as ecosystems remain in incomplete states of recovery. Aquatic organisms are thus faced with fewer sequential years under degraded environmental conditions to prepare for increasingly severe droughts and potential drying events. We assessed the effect of droughts and sex on the growth, mass, and mass-dependent estivation potential of long-lived aquatic salamanders (Greater Sirens, Siren lacertina) that estivate during drying events brought on by severe droughts. We calculated growth rates of S. lacertina based on mark–recapture data spanning …

6k1, Nia-Vpg, Nia-Pro, And Cp Ofwheat Streak Mosaic Virus Are Collective Determinants Of Wheat Streak Mosaic Disease In Wheat, Satyanarayana Tatineni, Jeffrey Alexander, Haritha Nunna

Department of Plant Pathology: Faculty Publications

Wheat streak mosaic virus (WSMV; genus Tritimovirus, family Potyviridae) is the causal agent of the most economically important wheat streak mosaic disease of wheat (Triticum aestivum) in the Great Plains region of theUnited States.WSMVdeterminants responsible forwheat streak mosaic disease in wheat are unknown. Triticum mosaic virus (TriMV), a wheatinfecting virus,was used as an expression vector for the transient expression of each of the WSMV-encoded cistrons in wheat. WSMV-encoded 6K1, NIa-VPg, NIa-Pro, and CP cistrons in TriMV elicited symptoms specific to different stages of wheat streak mosaic disease without significantly affecting the genomic RNA accumulation. WSMV 6K1 …