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2021

Yeast

Articles 1 - 16 of 16

Full-Text Articles in Life Sciences

Mitochondrial Contact Site And Cristae Organizing System (Micos) Machinery Supports Heme Biosynthesis By Enabling Optimal Performance Of Ferrochelatase, Jonathan V. Dietz, Mathilda M. Willoughby, Robert B. Piel, Teresa A. Ross, Iryna Bohovych, Hannah G. Addis, Jennifer L. Fox, William N. Lanzilotta, Harry A. Dailey, James A. Wohlschlegel, Amit R. Reddi, Amy E. Medlock, Oleh Khalimonchuk Oct 2021

Mitochondrial Contact Site And Cristae Organizing System (Micos) Machinery Supports Heme Biosynthesis By Enabling Optimal Performance Of Ferrochelatase, Jonathan V. Dietz, Mathilda M. Willoughby, Robert B. Piel, Teresa A. Ross, Iryna Bohovych, Hannah G. Addis, Jennifer L. Fox, William N. Lanzilotta, Harry A. Dailey, James A. Wohlschlegel, Amit R. Reddi, Amy E. Medlock, Oleh Khalimonchuk

Department of Biochemistry: Faculty Publications

Heme is an essential cofactor required for a plethora of cellular processes in eukaryotes. In metazoans the heme biosynthetic pathway is typically partitioned between the cytosol and mitochondria, with the first and final steps taking place in the mitochondrion. The pathway has been extensively studied and its biosynthetic enzymes structurally characterized to varying extents. Nevertheless, understanding of the regulation of heme synthesis and factors that influence this process in metazoans remains incomplete. Therefore, we investigated the molecular organization as well as the physical and genetic interactions of the terminal pathway enzyme, ferrochelatase (Hem15), in the yeast Saccharomyces cerevisiae. Biochemical and …

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Assessment Of Non-Saccharomyces Yeast Species As Secondary Fermenters In Beer, Paul J. Clark Sep 2021

Assessment Of Non-Saccharomyces Yeast Species As Secondary Fermenters In Beer, Paul J. Clark

Master of Science in Integrative Biology Theses

Secondary fermentation of beer is traditionally used to condition and refine the sensory profile of a beer. During methods such as barrel aging or lagering, the yeast continues to produce flavor-active compounds. Some methods include the intentional inoculation of microorganisms or the utilization of natural consortia associated with a barrel, fruit, or additive for the generation of desired sensory profiles. As unique products increase in popularity, brewers have begun to experiment with secondary fermentation using alternative yeast species as bioflavorants. To assess the viability of an organism’s application to brewing, the physiochemical properties of beer and their impact on the …

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Every Part Of Yeast Is The Best Part, Matthieu Baulez, Bruno Bertaud, Lisa Saibi Aug 2021

Every Part Of Yeast Is The Best Part, Matthieu Baulez, Bruno Bertaud, Lisa Saibi

Proceedings of the Arkansas Nutrition Conference

Yeasts are single-cell, eukaryotic microorganisms classified in the fungi kingdom. They are generally around 10μm in size, have a nuclear membrane, a cell wall, and a cytoplasmic content. Yeasts are characterized as heterotrophs, which means they rely on organic material as sources of energy and nutrients. Not all yeasts are equal. There are about 60 genera and about 1,500 species of yeasts. Only a few are used commercially. Thanks to its exceptional fermentative capacities and nutritional properties, Saccharomyces cerevisiae is the main yeast used in food, beverage and baking as well as in animal nutrition. The main types of yeasts …

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Investigating Stop Codon Readthrough In Saccharomyces Cerevisiae, Amy Kwon Aug 2021

Investigating Stop Codon Readthrough In Saccharomyces Cerevisiae, Amy Kwon

Undergraduate Student Research Internships Conference

Stop codon readthrough occurs via genetic and epigenetic mechanisms, resulting in a longer polypeptide chain at the C-terminus. Although these readthroughs may seem like an error made by translational mechanisms, evidence from yeast suggests that stop codon readthrough has an impact on various cellular processes. Readthrough has the potential to create genetic diversity, similar to RNA splicing, mRNA editing, and protein modification. The diversity created by translational readthrough may result in a beneficial change in phenotype, and thus have a role in evolution and adaptability. Translational readthrough is observed in organisms such as yeast and E. coli but may also …

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How Acetylation Regulates Metabolic Enzyme Function During Environmental Shifts, Jared Canonigo May 2021

How Acetylation Regulates Metabolic Enzyme Function During Environmental Shifts, Jared Canonigo

Biological Sciences Undergraduate Honors Theses

Organisms such as Saccharomyces cerevisiae can regulate the mechanisms of proteins through post-translational modification. These modifications play a vital role in functional proteomic activity because they can regulate protein activity, localization, and interaction with other cellular molecules. Such modifications include phosphorylation, methylation, and acetylation. The metabolic mechanisms of yeast became of keen interest to our lab because our lab noticed many stress defense proteins were being acetylated during stress heat shock. Notably, Adh1p and Adh2p showed both an increase and a decrease in acetylation at two lysine residues (K315 and K314) overtime during heat shock respectively, though the exact function …

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Increasing Stress Tolerance In Saccharomyces Cerevisiae Using Lipocalin Genes, Ximena Leon May 2021

Increasing Stress Tolerance In Saccharomyces Cerevisiae Using Lipocalin Genes, Ximena Leon

Honors Theses

Saccharomyces cerevisiae is a microorganism that is commonly used in industries, but its productivity is significantly hindered by its inability to resist high stress levels found in industrial processes. By increasing stress tolerance in S. cerevisiae, its role in industrial processes could be maximized. The objective of this study is to determine if complementation of lipocalin genes into the wildtype and knockout strains of S. cerevisiae can increase stress tolerance against a variety of stressors. We identified the knockout yeast strains, ALD3, ALD4, PDX3, and ILV1, as being sensitive to various stressors including salinity, heat, oxidative, and osmotic stresses. Arabidopsis …

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Evolution And Selection: From Suppression Of Metabolic Deficiencies To Bacteriophage Host Range And Resistance, Daniel Kurt Arens Apr 2021

Evolution And Selection: From Suppression Of Metabolic Deficiencies To Bacteriophage Host Range And Resistance, Daniel Kurt Arens

Theses and Dissertations

The evolution and adaptation of microorganisms is so rapid it can be seen in the time frame of days. The root cause for their evolution comes from selective environmental pressures that see organisms with beneficial mutations survive otherwise deadly encounters or outperform members of its population who fail to adapt. This does not always result in strict improvement of the individual as in the case of antibiotic resistant bacteria who often display fitness tradeoffs to avoid death (see Reviews [1-3]). For example, when an ampicillin resistance gene (ampC) containing plasmid that is occasionally found in the wild was transformed into …

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Investigation Of Fermentation Efficiency In Saccharomyces Cerevisiae Through Telomere Integrity And Maltase Expression, Dana Bennett Jan 2021

Investigation Of Fermentation Efficiency In Saccharomyces Cerevisiae Through Telomere Integrity And Maltase Expression, Dana Bennett

All Graduate Theses, Dissertations, and Other Capstone Projects

Many microbreweries practice serial fermentation, or serial repitching. Serial repitching is the process by which one yeast culture is reused for multiple batches of beer. Each batch of subsequent beer is called a “pitch.” This technique helps breweries limit production costs. However, fermentation is difficult to predict throughout serial fermentation. This leads to beer quality issues such as fluctuation in alcohol production, buildup of unwanted flavor compounds, and decreased carbon dioxide production, which results in reduced profit. To combat this issue, many breweries will attempt to predict fermentation efficiency through viable cell counting in order to ensure a consistent number …

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Evaluation Of Agrimune Liquid On Growth Performance Of Commercial Nursery Pigs, Andres F. Tolosa, Joel M. Derouchey, Jason C. Woodworth, Mike D. Tokach, Robert D. Goodband, Jordan T. Gebhardt Jan 2021

Evaluation Of Agrimune Liquid On Growth Performance Of Commercial Nursery Pigs, Andres F. Tolosa, Joel M. Derouchey, Jason C. Woodworth, Mike D. Tokach, Robert D. Goodband, Jordan T. Gebhardt

Kansas Agricultural Experiment Station Research Reports

A total of 1,188 pigs (L337 × 1050; PIC; initial BW of 11.1 lb) were used in a 40-d trial to determine the effect of a combination of mannan oligosaccharides, yeast culture, and beta-glucans (AgriMune Liquid; AFI-Agri Feed International, L.L.C., Rockwell, IA) on growth performance, morbidity, and mortality of pigs during the nursery period. Pigs were weaned at approximately 21 d of age and placed in pens (27 pigs per pen) based on initial BW. Pens were randomly assigned to 1 of 2 treatments in a randomized complete block design with 22 pens (replications) per treatment. Treatments consisted of the …

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The Effect Of Live Yeast And Yeast Extracts On Growth Performance Of Nursery Pigs Weaned From Sows Fed Diets With Or Without Yeast Additives, Jenna A. Chance, Jordan T. Gebhardt, Joel M. Derouchey, Mike D. Tokach, Jason C. Woodworth, Robert D. Goodband, Joseph A. Loughmiller Jan 2021

The Effect Of Live Yeast And Yeast Extracts On Growth Performance Of Nursery Pigs Weaned From Sows Fed Diets With Or Without Yeast Additives, Jenna A. Chance, Jordan T. Gebhardt, Joel M. Derouchey, Mike D. Tokach, Jason C. Woodworth, Robert D. Goodband, Joseph A. Loughmiller

Kansas Agricultural Experiment Station Research Reports

A total of 340 weaned pigs (241 × 600, DNA; initially 11.2 lb BW) were used in a 45-d study to evaluate previous sow treatment (control vs. yeast additives) and nursery diets with or without added yeast-based pre- and probiotics (Phileo by Lesaffre, Milwaukee, WI) on growth performance. At placement in the nursery, pigs were housed by pen based on previous sow treatment and randomly assigned to 1 of 2 dietary treatments with 5 pigs per pen and 17 replications per treatment. Treatments were arranged in a 2 × 2 factorial with main effects of sow treatment (control vs. yeast-based …

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The Effect Of Live Yeast And Yeast Extracts Included In Lactation Diets On Sow And Litter Performance, Jenna A. Chance, Jordan T. Gebhardt, Joel M. Derouchey, Mike D. Tokach, Jason C. Woodworth, Robert D. Goodband, Joseph A. Loughmiller, Brian Hotze Jan 2021

The Effect Of Live Yeast And Yeast Extracts Included In Lactation Diets On Sow And Litter Performance, Jenna A. Chance, Jordan T. Gebhardt, Joel M. Derouchey, Mike D. Tokach, Jason C. Woodworth, Robert D. Goodband, Joseph A. Loughmiller, Brian Hotze

Kansas Agricultural Experiment Station Research Reports

A total of 80 sows (Line 241; DNA Genetics) across three farrowing groups were used in a study to evaluate the effect of feeding live yeast and yeast extracts to lactating sows on sow and litter performance. Sows were blocked by BW and parity on d 110 of gestation and allotted to 1 of 2 dietary treatments. Dietary treatments consisted of a standard corn-soybean meal lactation diet or a diet that contained yeast-based preand probiotics (0.10% Actisaf Sc 47 HR+ and 0.025% SafMannan; Phileo by Lesaffre, Milwaukee, WI). Diets were fed from d 110 of gestation until weaning (approximately d …

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Effects Of Live Yeast And Yeast Extracts With And Without Pharmacological Levels Of Zinc On Nursery Pig Growth Performance And Fecal Consistency, Jenna A. Chance, Jordan T. Gebhardt, Joel M. Derouchey, Hilda I. Calderón, Mike D. Tokach, Jason C. Woodworth, Robert D. Goodband, Joseph A. Loughmiller Jan 2021

Effects Of Live Yeast And Yeast Extracts With And Without Pharmacological Levels Of Zinc On Nursery Pig Growth Performance And Fecal Consistency, Jenna A. Chance, Jordan T. Gebhardt, Joel M. Derouchey, Hilda I. Calderón, Mike D. Tokach, Jason C. Woodworth, Robert D. Goodband, Joseph A. Loughmiller

Kansas Agricultural Experiment Station Research Reports

A total of 360 weanling barrows (Line 200 × 400, DNA Genetics; initial BW 12.4 ± 0.05 lb) were used in a 42-d study to evaluate yeast-based pre- and probiotics (Phileo by Lesaffre, Milwaukee, WI) in diets with or without pharmacological levels of Zn on growth performance and fecal dry matter (DM). Pens were assigned to 1 of 4 dietary treatments with 5 pigs per pen and 18 pens per treatment. Dietary treatments were arranged in a 2 × 2 factorial with main effects of live yeast-based pre- and probiotics (none vs. 0.10% ActiSaf Sc 47 HR+, 0.05% SafMannan, and …

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Effects Of Live Yeast And Yeast Extracts With And Without Pharmacological Levels Of Zinc On Antimicrobial Susceptibilities Of Fecal Escherichia Coli In Nursery Pigs, Jenna A. Chance, Jordan T. Gebhardt, Joel M. Derouchey, Raghavendra G. Amachawadi, Victor Ishengoma, T. G. Nagaraja, Qing Kang, Mike D. Tokach, Jason C. Woodworth, Robert D. Goodband, Joseph A. Loughmiller Jan 2021

Effects Of Live Yeast And Yeast Extracts With And Without Pharmacological Levels Of Zinc On Antimicrobial Susceptibilities Of Fecal Escherichia Coli In Nursery Pigs, Jenna A. Chance, Jordan T. Gebhardt, Joel M. Derouchey, Raghavendra G. Amachawadi, Victor Ishengoma, T. G. Nagaraja, Qing Kang, Mike D. Tokach, Jason C. Woodworth, Robert D. Goodband, Joseph A. Loughmiller

Kansas Agricultural Experiment Station Research Reports

A total of 360 weanling barrows (Line 200 × 400, DNA Genetics; initial BW 12.4 ± 0.05 lb) were used in a 42-d study to evaluate yeast-based pre- and probiotics (Phileo by Lesaffre, Milwaukee, WI) in diets with or without pharmacological levels of Zn on antimicrobial resistance (AMR) patterns of fecalEscherichia coli. Pens were assigned to 1 of 4 dietary treatments with 5 pigs per pen and 18 pens per treatment. Dietary treatments were arranged in a 2 × 2 factorial with main effects of live yeast-based pre- and probiotics (none vs. 0.10% ActiSafSc 47 HR+, 0.05% SafMannan, …

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Mapping Genetic Variants Associated With Dynamic Protein Abundance In Haploid Yeast, Tanner Thuet-Davenport Jan 2021

Mapping Genetic Variants Associated With Dynamic Protein Abundance In Haploid Yeast, Tanner Thuet-Davenport

WWU Graduate School Collection

As organisms respond to changes in their environment genetic variation between individuals can directly affect organismal trait phenotypes by altering gene expression. Historically, studies have focused on the effect of genetic variation on mRNA synthesis (transcription) and decay rates. Relatively few studies have probed the relationship between DNA variants and protein-specific regulation of individual genes, despite the plethora of evidence that RNA levels are often poor proxies for protein levels. No study to date has mapped genetic variation associated with dynamic protein levels. In this study we investigated the location and identity of genetic variants acting on protein expression dynamics …

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The Effect Of Live Yeast And Yeast Extracts Included In Lactation Diets On Antimicrobial Susceptibility Of Fecal Escherichia Coli In Sows, Jenna A. Chance, Jordan T. Gebhardt, Joel M. Derouchey, Raghavendra G. Amachawadi, Victor Ishengoma, T. G. Nagaraja, Mike D. Tokach, Jason C. Woodworth, Robert D. Goodband, Qing Kang, Joseph A. Loughmiller, Brian Hotze Jan 2021

The Effect Of Live Yeast And Yeast Extracts Included In Lactation Diets On Antimicrobial Susceptibility Of Fecal Escherichia Coli In Sows, Jenna A. Chance, Jordan T. Gebhardt, Joel M. Derouchey, Raghavendra G. Amachawadi, Victor Ishengoma, T. G. Nagaraja, Mike D. Tokach, Jason C. Woodworth, Robert D. Goodband, Qing Kang, Joseph A. Loughmiller, Brian Hotze

Kansas Agricultural Experiment Station Research Reports

A total of 27 sows (Line 241; DNA Genetics) were used in a study to evaluate the effect of feeding live yeast and yeast extracts to lactating sows on antimicrobial susceptibilities of fecalE. coli. Sows were blocked by BW and parity on d 110 of gestation and allotted to 1 of 2 dietary treatments. Dietary treatments consisted of a standard corn-soybean meal lactation diet or a diet that contained yeast-based pre- and probiotics (0.10% Actisaf Sc 47 HR+ and 0.025% SafMannan; Phileo by Lesaffre, Milwaukee, WI). Diets were fed from d 110 of gestation until weaning (approximately d …

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The Effect Of Live Yeast And Yeast Extracts On Antimicrobial Susceptibilities Of Fecal Escherichia Coli Of Nursery Pigs Weaned From Sows Fed Diets With Or Without Yeast Additives, Jenna A. Chance, Jordan T. Gebhardt, Joel M. Derouchey, R. G. Amachawadi, V. Ishengoma, T. G. Nagaraja, M. D. Tokach, Jason C. Woodworth, Robert D. Goodband, Qing Kang, Joseph A. Loughmiller Jan 2021

The Effect Of Live Yeast And Yeast Extracts On Antimicrobial Susceptibilities Of Fecal Escherichia Coli Of Nursery Pigs Weaned From Sows Fed Diets With Or Without Yeast Additives, Jenna A. Chance, Jordan T. Gebhardt, Joel M. Derouchey, R. G. Amachawadi, V. Ishengoma, T. G. Nagaraja, M. D. Tokach, Jason C. Woodworth, Robert D. Goodband, Qing Kang, Joseph A. Loughmiller

Kansas Agricultural Experiment Station Research Reports

A total of 340 weaned pigs (Line 241 × 600, DNA; initially 11.2 lb BW) were used in a 45-d study to evaluate previous sow treatment (control vs. yeast additives) and nursery diets with or without added yeast-based pre- and probiotics (Phileo by Lesaffre, Milwaukee, WI) on antimicrobial resistance (AMR) patterns of fecalEscherichia coli. At placement in the nursery, pigs were housed by pen based on sow treatment and randomly assigned to 1 of 2 dietary treatments with 5 pigs per pen and 17 pens per treatment. Treatments were arranged in a 2 × 2 factorial with main …

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