Life Sciences Commons^™

Stimulation Of Ammonia Oxidizer And Denitrifier Abundances By Nitrogen Loading: Poor Predictability For Increased Soil N2o Emission, Yong Zhang, Feng Zhang, Diego Abalos, Yiqi Luo, Dafeng Hui, Bruce A. Hungate, Pablo García-Palacios, Yakov Kuzyakov, Jørgen Eivind Olesen, Uffe Jørgensen, Ji Chen

Biology Faculty Research

Unprecedented nitrogen (N) inputs into terrestrial ecosystems have profoundly altered soil N cycling. Ammonia oxidizers and denitrifiers are the main producers of nitrous oxide (N₂O), but it remains unclear how ammonia oxidizer and denitrifier abundances will respond to N loading and whether their responses can predict N-induced changes in soil N₂O emission. By synthesizing 101 field studies worldwide, we showed that N loading significantly increased ammonia oxidizer abundance by 107% and denitrifier abundance by 45%. The increases in both ammonia oxidizer and denitrifier abundances were primarily explained by N loading form, and more specifically, organic N …

Ancient Dna Of The Pygmy Marmoset Type Specimen Cebuella Pygmaea (Spix, 1823) Resolves A Taxonomic Conundrum, J. P. Boubli, M. C. Janiak, L. M. Porter, Stella De La Torre, L. Cortés-Ortiz, M. N. F. Da Silva, A. B. Rylands, Stephen Nash, F. Bertuol, H. Byrne, F. E. Silva, F. Rohe, D. De Vries, R. M. D. Beck, I. Ruiz-Gartzia, L. F. K. Kuderna, T. Marques-Bonet, Tomas Hrbek, I. P. Farias, A. H. Van Heteren, C. Roos

Biology Faculty Research

The pygmy marmoset, the smallest of the anthropoid primates, has a broad distribution in Western Amazonia. Recent studies using molecular and morphological data have identified two distinct species separated by the Napo and Solimões-Amazonas rivers. However, reconciling this new biological evidence with current taxonomy, i.e., two subspecies, Cebuella pygmaea pygmaea (Spix, 1823) and Cebuella pygmaea niveiventris (Lönnberg, 1940), was problematic given the uncertainty as to whether Spix’s pygmy marmoset (Cebuella pygmaea pygmaea) was collected north or south of the Napo and Solimões-Amazonas rivers, making it unclear to which of the two newly revealed species the name pygmaea would …

Mapping The Hidden Diversity Of The Geophagus Sensu Stricto Species Group (Cichlidae: Geophagini) From The Amazon Basin, A. M. Ximenes, P. S. Bittencourt, V. N. Machado, Tomas Hrbek, I. P. Farias

Biology Faculty Research

South American freshwater ichthyofauna is taxonomically the most diverse on the planet, yet its diversity is still vastly underestimated. The Amazon basin alone holds more than half of this diversity. The evidence of this underestimation comes from the backlog of morphologically distinct, yet undescribed forms deposited in museum collections, and from DNA-based inventories which consistently identify large numbers of divergent lineages within even well-studied species groups. In the present study, we investigated lineage diversity within the Geophagus sensu stricto species group. To achieve these objectives, we analyzed 337 individuals sampled from 77 locations within and outside the Amazon basin representing …

Synapsej: An Automated, Synapse Identification Macro For Imagej, Juan Felipe Moreno Manrique, Parker R. Voit, Kathryn E. Windsor, Aamuktha Reddy Karla, Sierra R. Rodriguez, Gerard M.J. Beaudoin Iii

Biology Faculty Research

While electron microscopy represents the gold standard for detection of synapses, a number of limitations prevent its broad applicability. A key method for detecting synapses is immunostaining for markers of pre- and post-synaptic proteins, which can infer a synapse based upon the apposition of the two markers. While immunostaining and imaging techniques have improved to allow for identification of synapses in tissue, analysis and identification of these appositions are not facile, and there has been a lack of tools to accurately identify these appositions. Here, we delineate a macro that uses open-source and freely available ImageJ or FIJI for analysis …

Short-Term Canopy And Understory Nitrogen Addition Differ In Their Effects On Seedlings Of Dominant Woody Species In A Subtropical Evergreen Broadleaved Forest, Jun Wang, Dafeng Hui, Hai Ren, Nan Liu, Zhongyu Sun, Long Yang, Hongfang Lu

Biology Faculty Research

As atmospheric nitrogen (N) deposition continues to increase, information on how N deposition affects seedling performance of tree species is critical for predicting forest regeneration. In this study, we examined the effects of canopy N addition at 25 and 50 kg ha−1 year−1 of N (CAN25 and CAN50), and understory N addition at 25 and 50 kg ha−1 year−1 of N (UAN25 and UAN50) on the survival, physiology, and growth of seedlings of two dominant woody species (Schima superba and Ardisia quinquegona) that were transplanted into a subtropical evergreen broadleaved forest. At two years after transplanting, understory N additon greatly …

Linking Ecomechanical Models And Functional Traits To Understand Phenotypic Diversity, T. E. Higham, L. A. Ferry, L. Schmitz, D. J. Irschick, S. Starko, P. S L Anderson, P. J. Bergmann, H. A. Jamniczky, L. R. Monteiro, D. Navon, J. Messier, E. Carrington, S. C. Farina, K. L. Feilich, L. P. Hernandez, Michele A. Johnson, S. M. Kawano, C. J. Law, S. J. Longo, C. H. Martin, P. T. Martone, A. Rico-Guevara, S. E. Santana, K. J. Niklas

Biology Faculty Research

Physical principles and laws determine the set of possible organismal phenotypes. Constraints arising from development, the environment, and evolutionary history then yield workable, integrated phenotypes. We propose a theoretical and practical framework that considers the role of changing environments. This 'ecomechanical approach' integrates functional organismal traits with the ecological variables. This approach informs our ability to predict species shifts in survival and distribution and provides critical insights into phenotypic diversity. We outline how to use the ecomechanical paradigm using drag-induced bending in trees as an example. Our approach can be incorporated into existing research and help build interdisciplinary bridges. Finally, …

Season Of Prescribed Fire Determines Grassland Restoration Outcomes After Fire Exclusion And Overgrazing, Erin N. Novak, Michelle Bertelsen, Dick Davis, Devin M. Grobert, Kelly G. Lyons, Jason P. Martina, W. Matt Mccaw, Matthew O'Toole, Joseph W. Veldman

Biology Faculty Research

Fire exclusion and mismanaged grazing are globally important drivers of environmental change in mesic C₄ grasslands and savannas. Although interest is growing in prescribed fire for grassland restoration, we have little long-term experimental evidence of the influence of burn season on the recovery of herbaceous plant communities, encroachment by trees and shrubs, and invasion by exotic grasses. We conducted a prescribed fire experiment (seven burns between 2001 and 2019) in historically fire-excluded and overgrazed grasslands of central Texas. Sites were assigned to one of four experimental treatments: summer burns (warm season, lightning season), fall burns (early cool season), winter …

Association Of Oral Microbiota With Lung Cancer Risk In A Low-Income Population In The Southeastern Usa, Jiajun Shi, Yaohua Yang, Hua Xie, Xiaofei Wang, Jie Wu, Jirong Long, Regina Courtney, Xiao-Ou Shu, Wei Zheng, William J. Blot, Qiuyin Cai

Biology Faculty Research

Purpose

Oral microbiome plays an important role in oral health and systemic diseases, including cancer. We aimed to prospectively investigate the association of oral microbiome with lung cancer risk.

Methods

We analyzed 156 incident lung cancer cases (73 European Americans and 83 African Americans) and 156 individually matched controls nested within the Southern Community Cohort Study. Oral microbiota were assessed using 16S rRNA gene sequencing in pre-diagnostic mouth rinse samples. Paired t test and the permutational multivariate analysis of variance test were used to evaluate lung cancer risk association with alpha diversity or beta diversity, respectively. Conditional logistic regression models …

Cancer Salt Nostalgia, Aashish S. Allu, Venkataswarup Tiriveedhi

Biology Faculty Research

High-salt (sodium chloride) diets have been strongly associated with disease states and poor health outcomes. Traditionally, the impact of salt intake is primarily studied in cardiovascular diseases, hypertension and renal diseases; however, recently there has been increasing evidence demonstrating the role of salt in autoimmune diseases. Salt has been shown to modulate the inflammatory activation of immune cells leading to chronic inflammation-related ailments. To date, there is minimal evidence showing a direct correlation of salt with cancer incidence and/or cancer-related adverse clinical outcomes. In this review article, we will discuss the recent understanding of the molecular role of salt, and …

Elevated Atmospheric Co2 Concentration Triggers Redistribution Of Nitrogen To Promote Tillering In Rice, Juan Zhou, Yingbo Gao, Junpeng Wang, Chang Liu, Zi Wang, Minjia Lv, Xiaoxiang Zhang, Yong Zhou, Guichun Dong, Yulong Wang, Jianye Huang, Dafeng Hui, Zefeng Yang, Youli Yao

Biology Faculty Research

Elevated atmospheric CO₂ concentration (eCO₂) often reduces nitrogen (N) content in rice plants and stimulates tillering. However, there is a general consensus that reduced N would constrain rice tillering. To resolve this contradiction, we investigated N distribution and transcriptomic changes in different rice plant organs after subjecting them to eCO₂ and different N application rates. Our results showed that eCO₂ significantly promoted rice tillers (by 0.6, 1.1, 1.7, and 2.1 tillers/plant at 0, 75, 150, and 225 kg N ha⁻¹ N application rates, respectively) and more tillers were produced under higher N application rates, …

Detection And Quantification Of Γ-H2ax Using A Dissociation Enhanced Lanthanide Fluorescence Immunoassay, Felicite K. Noubissi, Amber A. Mcbride, Hannah G. Leppert, Larry J. Millet, Xiaofei Wang, Sandra M. Davern

Biology Faculty Research

Phosphorylation of the histone protein H2AX to form γ-H2AX foci directly represents DNA double-strand break formation. Traditional γ-H2AX detection involves counting individual foci within individual nuclei. The novelty of this work is the application of a time-resolved fluorescence assay using dissociation-enhanced lanthanide fluorescence immunoassay for quantitative measurements of γ-H2AX. For comparison, standard fluorescence detection was employed and analyzed either by bulk fluorescent measurements or by direct foci counting using BioTek Spot Count algorithm and Gen 5 software. Etoposide induced DNA damage in A549 carcinoma cells was compared across all test platforms. Time resolved fluorescence detection of europium as a chelated …

Distribution And Conservation Status Of Camellia Longzhouensis (Theaceae), A Critically Endangered Plant Species Endemic To Southern China, Zhanhui Xu, Hai Ren, Xiao Wei, Kangting Ouyang, Dongxing Li, Yili Guo, Shujun Wen, Jifeng Long, Jun Wang, Dafeng Hui

Biology Faculty Research

Camellia longzhouensis (Theaceae) is an endemic evergreen shrub or small tree with a distribution restricted to South China. It is listed as Grade-II in the National Key Protected Wild Plants List. In this study, we surveyed its distribution, examined its population structure, identified factors affecting its survival, and reassessed its extinction risk. We found that C. longzhouensis was only distributed in the Nonggang National Nature Reserve and the surrounding area. Its individuals only grew under the secondary forest canopy in the karst mountain. A total of 58 individuals of C. longzhouensis in three sub-populations were found. Soil fertility and understory …

Ex Vivo High Salt Activated Tumor-Primed Cd4+T Lymphocytes Exert A Potent Anti-Cancer Response, Venkataswarup Tiriveedhi, Michael Ivy, Elbert L. Myles, Roy Zent, Jeffrey C. Rathmell, Jens M. Titze

Biology Faculty Research

Cell based immunotherapy is rapidly emerging as a promising cancer treatment. A modest increase in salt (sodium chloride) concentration in immune cell cultures is known to induce inflammatory phenotypic differentiation. In our current study, we analyzed the ability of salt treatment to induce ex vivo expansion of tumor-primed CD4 (cluster of differentiation 4)+T cells to an effector phenotype. CD4+T cells were isolated using immunomagnetic beads from draining lymph nodes and spleens from tumor bearing C57Bl/6 mice, 28 days post-injection of Py230 syngeneic breast cancer cells. CD4+T cells from non-tumor bearing mice were isolated from splenocytes of 12-week-old C57Bl/6 mice. These …

Culturable Root Endophyte Communities Are Shaped By Both Warming And Plant Host Identity In The Rocky Mountains, Usa, Kelly G. Lyons, M. Mann, Molly Lenihan, Olivia Roybal, Kelly Carroll, Kyle Reynoso, S. N. Kivlin, D. L. Taylor, J. A. Rudgers

Biology Faculty Research

Understanding the biogeographic patterns of root-associated fungi and their sensitivity to temperature may improve predictions of future changes in terrestrial biodiversity and associated ecosystem processes, but data are currently limited. Anticipating change will require combining observational data, which predict how climatic factors limit current species distributions, with direct manipulations of climate, which can isolate responses to specific climate variables. Root endophytes are common symbionts of plants, particularly in arctic and alpine environments, yet their responses to climate warming are not resolved. Here, we directly cultured endophytic fungi from roots collected along altitudinal gradients in replicated mountain watersheds and from a …

An Integrative Analysis Uncovers A New, Pseudo-Cryptic Species Of Amazonian Marmoset (Primates: Callitrichidae: Mico) From The Arc Of Deforestation, R. Costa-Araujo, J. S. Silva Jr., J. P. Boubli, R. V. Rossi, G. R. Canale, F. R. Melo, F. Bertuol, F. E. Silva, D. A. Silva, S. D. Nash, I. Sampaio, I. P. Farias, Tomas Hrbek

Biology Faculty Research

Amazonia has the richest primate fauna in the world. Nonetheless, the diversity and distribution of Amazonian primates remain little known and the scarcity of baseline data challenges their conservation. These challenges are especially acute in the Amazonian arc of deforestation, the 2500 km long southern edge of the Amazonian biome that is rapidly being deforested and converted to agricultural and pastoral landscapes. Amazonian marmosets of the genus Mico are little known endemics of this region and therefore a priority for research and conservation efforts. However, even nascent conservation efforts are hampered by taxonomic uncertainties in this group, such as the …