Life Sciences Commons^™

Development Of Paper-Based 3d Cell Culture Devices And Real-Time Biosensing Applications, Naricha Pupinyo

Chulalongkorn University Theses and Dissertations (Chula ETD)

Due to its micro fibrous pores, paper has gained traction in the use as a scaffold for 3D cell culture. To study melanoma mechanism, screening the amount of melanin production and invasion study are major keys for drug development. In this work, we developed paper-based devices for culturing melanoma cells. Through the development process, we obtained the optimal parameters, which were applied for 3 different projects; 1) Anti-melanogenic effect screening, 2) Impedance-based E-screen assay, and 3) A membrane insert for real-time invasion assay. As a result of melanin production from melanocytes, the cells can be easily observed after a few …

Effects And Mechanisms Of Caesalpinia Mimosoides Leaf Extract On Oxidative Stress Resistance And Anti-Aging Activity In Caenorhabditis Elegans And Neurite Outgrowth Activity In Neuro2a Cells, Panthakarn Rangsinth

Chulalongkorn University Theses and Dissertations (Chula ETD)

World’s population of older people is rising, dietary supplements promoting healthy lifespan are needed. Moreover, Alzheimer’s disease (AD), an age-related degenerative disease, becomes a public health problem in aging society. Recent reports using cell culture models of AD suggest that amyloid precursor protein (APP), a protein causally related to AD, plays an important role as an inhibitor of neurite outgrowth. Medicinal herbs with neurite outgrowth stimulatory effect may help to prevent and cure AD. Moreover, studies suggest that dietary supplements from plant sources act in preventive nutrition, since they provide antioxidant action against oxidative stress, promote healthspan and prolong lifespan. …

Associations Between Maternal Physical Activity In Early And Late Pregnancy And Offspring Birth Size: Remote Federated Individual Level Meta‐Analysis From Eight Cohort Studies, S. Pastorino, T. Bishop, S. Crozier, C. Granstrom, K. Kordas, L. Kupers, E. O'Brien, K. Polanska, K. Sauder, M. Zafarmand, R. Wilson, C. Agyemang, P. Burton, C. Cooper, E. Corpeleijn, D. Dabelea, W. Hanke, H. Inskip, F. Mcauliffe, S. Olsen, T. Vrijkotte, S. Brage, A. Kennedy, D. O'Gorman, P. Scherer, K. Wijndaele, N. Wareham, G. Desoye, K. K. Ong

Articles

Objective

Evidence on the impact of leisure time physical activity (LTPA) in pregnancy on birth size is inconsistent. We aimed to examine the association between LTPA during early and late pregnancy and newborn anthropometric outcomes.

Design

Individual level meta‐analysis, which reduces heterogeneity across studies.

Setting

A consortium of eight population‐based studies (seven European and one US) comprising 72 694 participants.

Methods

Generalised linear models with consistent inclusion of confounders (gestational age, sex, parity, maternal age, education, ethnicity, BMI, smoking, and alcohol intake) were used to test associations between self‐reported LTPA at either early (8–18 weeks gestation) or late pregnancy (30+ …

Cost-Efficient And Easy To Perform Pcr-Based Assay To Identify Met Exon 14 Skipping In Formalin-Fixed Paraffin-Embedded (Ffpe) Non-Small Cell Lung Cancer (Nsclc) Samples, Odharnaith O'Brien, Mark C. Wright, Cathal O'Brien, Orla Geoghegan, Niamh Leonard, Siobhan Nicolson, Sinead Cuffe, Aurelie Fabre, Wolfram Jochum, Markus Joerger, Steven G. Gray, Stephen P. Finn

Articles

MET is a receptor tyrosine kinase (RTK) that plays important roles in carcinogenesis. Despite being frequently overexpressed in cancer, clinical responses to targeting this receptor have been limited. Recently novel splicing mutations involving the loss of exon 14 (called METex14 skipping) have emerged as potential biomarkers to predict for responsiveness to targeted therapies with Met inhibitors in non-small cell lung cancer (NSCLC). Currently, the diverse genomic alterations responsible for METex14 skipping pose a challenge for routine clinical diagnostic testing. In this report, we examine three different methodologies to detect METex14 and assess their potential utility for use as a diagnostic …

Efficacy Of Cold Plasma For Direct Deposition Of Antibiotics As A Novel Approach For Localized Delivery And Retention Of Effect, Agatha Los, Dana Ziuzina, Daniela Boehm, Lu Han, Denis O'Sullivan, Liam O'Neill, Paula Bourke

Articles

Antimicrobial coating of medical devices has emerged as a potentially effective tool to prevent or ameliorate device-related infections. In this study the plasma deposition process for direct deposition of pharmaceutical drugs on to a range of surfaces and the retention of structure function relationship and antimicrobial efficacy against mono-species biofilms were investigated. Two selected sample antibiotics—ampicillin and gentamicin, were deposited onto two types of surfaces—polystyrene microtiter plates and stainless steel coupons. The antimicrobial efficacy of the antibiotic-coated surfaces was tested against challenge populations of both planktonic and sessile Escherichia coli and Pseudomonas aeruginosa, with responses monitored for up to …

An Observational Analysis Of Meal Patterns In Overweight And Obese Pregnancy, Kate M. Ainscough, Maria A. Kennelly, Eileen C. O'Brien, Elizabeth J. O'Sullivan, John Mehegan, Eileen R. Gibney, Fionnauala M. Mcauliffe

Articles

Background Nutrient intakes are known to be poorer among pregnant women with raised body mass index (BMI) than those with a healthy BMI. While meal patterns have the potential to influence obstetric, metabolic and anthropometric measures for mother and infant, limited data exists regarding meal patterns among pregnant women with raised BMI.

Aim To identify categories of meal patterns among pregnant women with overweight and obesity and determine whether patterns change with advancing gestation. To determine if maternal meal patterns are associated with dietary intakes and pregnancy outcomes.

Methods Prospective, observational analysis of pregnant women (n = 143) (BMI 25–39.9 …

Biological Membranes In Ev Biogenesis, Stability, Uptake, And Cargo Transfer: An Isev Position Paper Arising From The Isev Membranes And Evs Workshop, Ashley E. Russell, Alexandra Sneider, Kenneth W. Witwer, Paola Bergese, Suvendra N. Bhattacharyya, Alexander Cocks, Emanuele Cocucci, Uta Erdbrugger, Juan M. Falcon-Perez, David W. Freeman, Thomas M. Gallagher, Shuaishuai Hu, Yiyao Huang, Steven M. Jay, Shin-Ichi Kano, Gregory Lavieu, Aleksandra Leszczynska, Alicia M. Llorente, Quan Lu, Vasiliki Mahairaki, Dillon C. Muth, Nicole Noren Hooten, Matias Ostrowski, Ilaria Prada, Susmita Sahoo, Tine Hiroth Schoyen, Lifu Sheng, Deanna Tesch, Guillaume Van Niel, Roosmarijn E. Vandenbroucke, Frederik J. Verweij, Ana V. Villar, Marca Wauben, Hang Yin, David Raul Francisco Carter, Pieter Vader

Articles

Paracrine and endocrine roles have increasingly been ascribed to extracellular vesicles (EVs) generated by multicellular organisms. Central to the biogenesis, content, and function of EVs are their delimiting lipid bilayer membranes. To evaluate research progress on membranes and EVs, the International Society for Extracellular Vesicles (ISEV) conducted a workshop in March 2018 in Baltimore, Maryland, USA, bringing together key opinion leaders and hands-on researchers who were selected on the basis of submitted applications. The workshop was accompanied by two scientific surveys and covered four broad topics: EV biogenesis and release; EV uptake and fusion; technologies and strategies used to study …

Disarming Pseudomonas Aeruginosa Virulence By The Inhibitory Action Of 1,10-Phenanthroline-5,6-Dione- Based Compounds: Elastase B (Lasb) As A Chemotherapeutic Target, Anna Clara M. Galdino, Livia Vigano, Aleaxandre A. De Castron, Elaine F.F; Da Cunha, Thais P. Mello, Larissa M. Mattos, Marcos D. Pereira, Mary Hunt, Megan O'Shaughnessy, Orla L. Howe, Michael Devereux, Malachy Mccann, Teodorico C. Ramalho, Marta H. Branquinha, André L.S. Santos

Articles

negative pathogen Pseudomonas aeruginosa, and this enzyme orchestrates several physiopathological events during bacteria-host interplays. LasB is considered to be a potential target for the development of an innovative chemotherapeutic approach, especially against multidrug-resistant strains. Recently, our group showed that 1,10-phenanthroline-5,6-dione (phendione), [Ag(phendione)2]ClO4 (Ag-phendione) and [Cu(phendione)3](ClO4)2.4H2O (Cu-phendione) had anti-P. aeruginosa action against both planktonic- and biofilm-growing cells. In the present work, we have evaluated the effects of these compounds on the (i) interaction with the lasB active site using in silico approaches, (ii) lasB proteolytic activity by using a specific fluorogenic peptide substrate, (iii) lasB gene expression by real time-polymerase chain …

The Abundance Of Akkermansia Muciniphila And Its Relationship With Sulphated Colonic Mucins In Health And Ulcerative Colitis, Helen Earley, Grainne Earley, Aine Balfe, J. Calvin Coffey, Desmond C. Winter, Ronan O'Connell

Articles

Akkermansia muciniphila utilises colonic mucin as its substrate. Abundance is reduced in ulcerative colitis (UC), as is the relative proportion of sulphated mucin in the mucus gel layer (MGL). It is unknown if these phenomena are related, however reduced sulphated mucins could contribute to reduced abundance, owing to a lack of substrate. The aim of this study was to quantify A. muciniphila within the MGL and to relate these findings with markers of inflammation and the relative proportion of sulphomucin present. Colonic biopsies and mucus brushings were obtained from 20 patients with active UC (AC), 14 with quiescent UC (QUC) …

The Relationship Between Maternal Plasma Homocysteine In Early Pregnancy And Birth Weight, Shona Cawley, Eimer O'Malley, Rachel Kennedy, Ciara Reynolds, Anne Molloy, Michael Turner

Articles

Background: There is limited evidence that plasma homocysteine (Hcy) is increased in women with adverse pregnancy outcomes, such as low birth weight (LBW).

Objective: We examined the relationship between maternal Hcy at the first prenatal visit and birth weight.

Study design: In a prospective observational study, women were recruited during their first prenatal visit after sonographic confirmation of gestational age. Along with the standard tests, blood was also taken for the measurement of maternal serum and red blood cell (RBC) folate, vitamin B12, and Hcy. In addition to collecting standard clinical and sociodemographic details, a detailed questionnaire on vitamin supplementation …

Subjective And Objective Assessment Of Taste And Smell Sensation In Advanced Cancer, Niamh Mcgettigan, Pauline Uí Dhuibhir, Michelle Barrett, Jessica Sui, Lucy Balding, Stephen Higgins, Norma O'Leary, Aileen Kennedy, Declan Walsh

Articles

Context: Taste and smell abnormalities (TSA) occur throughout the cancer trajectory regardless of cancer primary site and contribute to cancer-associated malnutrition. TSA etiology is poorly understood. Tumor-related inflammation is a possible cause. Objective: This study examined the prevalence, characteristics, and severity of TSA in advanced cancer and explored the relationship between TSA and nutritional status. No previous study combined subjective and objective measures for both taste and smell assessment in this population. Method: Consecutive advanced cancer hospice patients were recruited. A modified version of the “Taste and Smell Survey” assessed subjective TSA. Validated taste strips and “Sniffin’ …

Development And Diagnostics Of Novel Non-Thermal Plasmatreatment Systems, Laurence Scally

Doctoral

Non-thermal plasma (NTP) has been a point of interest in many areas over the last few decades. Much research has been,and continues to beundertaken,to understand the fundamentals of plasma discharges. This is such a broad topic due to the very nature and variable dependencies that set the conditions for plasma discharge to occur. This can come in the form of electrode geometry and spacing, dielectric barrier thickness, humidity of environment, material selection for electrodes and dielectric barriers, the power supply used, and the operating gas(es) used. A lot of these influencing factors can be set and kept constant, but still …

Neutrosophic Cubic Heronian Mean Operators With Applications In Multiple Attribute Group Decision-Making Using Cosine Similarity Functions, Muhammad Gulistan, Mutaz Mohammad, Faruk Karaaslan, Seifedine Kadry, Salma Khan, Hafiz Abdul Wahab

All Works

© The Author(s) 2019. This article introduces the concept of Heronian mean operators, geometric Heronian mean operators, neutrosophic cubic number–improved generalized weighted Heronian mean operators, neutrosophic cubic number–improved generalized weighted geometric Heronian mean operators. These operators actually generalize the operators of fuzzy sets, cubic sets, and neutrosophic sets. We investigate the average weighted operator on neutrosophic cubic sets and weighted geometric operator on neutrosophic cubic sets to aggregate the neutrosophic cubic information. After this, based on average weighted and geometric weighted and cosine similarity function in neutrosophic cubic sets, we developed a multiple attribute group decision-making method. Finally, we give …

Loop-Mediated Isothermal Amplification In The Field: Reality Beyond The Potentiality, Donato Antonio Raele, Nicola Pugliese, Domenico Galante, Maria Assunta Cafiero

Turkish Journal of Veterinary & Animal Sciences

No abstract provided.

A Comparative Phylotyping Analysis Of E. Coli Originating From Avian Colibacillosis Based On The Clermont Triplex Scheme And Gyra Gene Sequencing, Mohsen Amiri, Elham Ahmadi

Turkish Journal of Veterinary & Animal Sciences

Avian colibacillosis (AC) is frequently reported from poultry farms in the west of Iran. The aim of the present survey was to evaluate the phylogeny of Escherichia coli isolated from AC through the Clermont triplex protocol and gyrA gene sequencing. One hundred and fifty fresh carcasses suspected of having died from colibacillosis were sampled. The specimens were examined for E. coli isolation and identification. The extracted DNA repertoire of the isolates was subjected to phylogenetic analysis using Clermont triplex PCR. A DNA fragment of 1.8 kbp of the gyrA gene was amplified from a representative of each phylogroup or subgroup …

Methods Of Diagnosis And Treatment Of Postpartum Uterine Infection Adopted By Algerian Veterinarians, Hamza Rahab, Alessandro Ricci, Ahmed Kasseh-Laouar, Christian Hanzen

Turkish Journal of Veterinary & Animal Sciences

The present study aimed to monitor the practices of Algerian veterinarians in the diagnosis and treatment of puerperal metritis (PM) and clinical endometritis (CE) in cattle. For this purpose, a questionnaire was established and distributed to collect the information concerning veterinarian profiles and practices related to the diagnosis and treatment of PM and CE. The results showed that examinations of the uterus (rectal palpation), vaginal discharge, and rectal temperature were the most practiced methods for PM (71%, 68%, and 83%, respectively) and CE (86%, 60%, and 65%, respectively) diagnosis. Tetracycline was the antibiotic of choice for intrauterine treatment of PM …

Effects Of The Feeds Containing Hazelnut Meal As Plant Protein Source On Growth Performance And Body Composition Of Siberian Sturgeon (Acipenser Baeri) And Economic Profitability Value, Huri̇ye Ariman Karabulut, İlker Zeki̇ Kurtoğlu, Yunus Emre Kirtan

Turkish Journal of Veterinary & Animal Sciences

In this study, the effects of feeding with the diets containing hazelnut meal (HM) at different levels (0%, 15%, 30%, and 45%) on the growth performance and body composition of Siberian sturgeon were investigated. The study was carried out for 90 days with 3 replicates (n = 15; 283.1 ± 6.52 g fish weight). The highest weight gain (180.62 ± 2.84%) was observed in the control group followed by 15%, 30%, and 45% HM. When the groups were evaluated in terms of SGR, FCR, and PER, the control group was observed to have the best performance. The control group and …

Evaluation Of Milk Glutathione Peroxidase And Superoxide Dismutase Levels In Subclinical Mastitis In Damascus Goats, İsfendi̇yar Darbaz, Seçki̇n Salar, Serkan Sayiner, İdi̇l Baştan, Osman Ergene, Ayhan Baştan

Turkish Journal of Veterinary & Animal Sciences

The aim of this study was to evaluate milk glutathione peroxidase (GPx) and superoxide dismutase (SOD) levels in Damascus goats with subclinical mastitis. According to the somatic cell counts (SCCs), 193 Damascus goats included in the study were divided into healthy (n = 75; SCC 0.05). In summary, a significant increase in GPx and decrease in SOD levels, respectively, were determined in goat milk samples with subclinical mastitis, and these changing trends were correlated with milk SCCs. However, age, parity, and number of offspring were not associated with these changing patterns.

Electrocardiogram And Serum Cardiac Biomarkers Changes In Fmd In Cattle, Ali Abbas Nikvand, Seyedeh Missagh Jalali, Mohammad Nouri, Alireza Ghadrdan Mashhadi, Soroush Hassanpour Amirabadi

Turkish Journal of Veterinary & Animal Sciences

This study was designed to evaluate serum and electrocardiographic (ECG) changes in cattle with foot-and-mouth disease (FMD). Thirty-two cows with clinical signs of FMD and 13 healthy cows were randomly enrolled. After blood sampling, ECG was performed on 23 of the 32 ill cows. Serum circulatory troponin I (cTnI), CPK, LDH, and CK-MB activities were determined in the patient and control groups. Arrhythmias including ventricular tachycardia (VT), sinus tachycardia (ST), atrial fibrillation (AF), and premature ventricular complex (PVC) were seen in 4, 4, 2, and 1 of the patients, respectively. The patients had higher cTnI levels than the control group …

Prolonged Gestation In A Swedish Red Cow Due To Fetal Unilateral Adrenal Gland Dysgenesis, Ebru Karakaya-Bi̇len, Ahmet Akkoç, Bariş Güner, Gülnaz Yilmazbaş-Meci̇toğlu, Ahmet Gümen, Abdulkadi̇r Keski̇n

Turkish Journal of Veterinary & Animal Sciences

A 5-year-old Swedish Red cow, pregnant for 465 days according to the last insemination record, was presented in this case. She conceived by artificial insemination at spontaneous estrus using frozen-thawed sperm of Swedish Red sire. No abnormalities were detected during gestation and no clinical signs of parturition at the expected time of delivery were noted. She was reexamined several times to eliminate breeding records error and, once prolonged gestation was confirmed, parturition was induced. The calf survived birth but died 10 min after birth. A macroscopically giant fetus was diagnosed. Following necropsy, some congenital anomalies were noticed. While the structure …

Effects Of The Inclusion Of Detoxified Argan Press Cake In The Diet Of Dairy Goats On Milk Production And Milk Quality, Nazha Lakram, Sana Moutik, Ikram Mercha, El Haj El Maadoudi, Rachid Kabbour, Ahmed Douaik, Abdelmajid Zouahri, Abdelah El Housni, Mariam Naciri

Turkish Journal of Veterinary & Animal Sciences

This study was carried out to assess the effect of the incorporation of detoxified argan press cake on milk yield, physicochemical composition, and microbial quality of raw Alpine goat milk produced in the Meskala-Essaouira region of southwestern Morocco. The detoxification method, adopted for the removal of saponin present in the argan press cake, succeeded in reducing these antinutrients from 4.56 to 0.4 mg/g, allowing it to be used as livestock feed. The average of milk production increased by 24% for the detoxified argan press cake (DAPC) group compared to the argan press cake and local diet groups. The diets and …

Anesthetic And Sedative Efficacy Of Peppermint (Mentha Piperita) And Lavender (Lavandula Angustifolia) Essential Oils In Blue Dolphin Cichlid (Cyrtocara Moorii), Erkan Can, Esma Sümer

Turkish Journal of Veterinary & Animal Sciences

The use of different essential oils (Eo$_{s}$), which are considered as a good alternative to synthetic materials, is increasing day by day in fish species. Nevertheless, studies on the anesthetic effect of peppermint Eo (Mentha piperita; MPEo) and lavender Eo (Lavandula angustifolia; LAEo) are scarce. In the present study, the effective concentrations of three anesthetic materials (MPEo, LAEo, and MS-222) were previously determined in the concentrations range of 100-400 ppm in Cyrtocara moorii juveniles (4.92 ± 1.03 g and 7.13 ± 1.22 cm) with the criteria of appropriate induction time (AI$_{t}$) and ready recovery time (RRt) (Trial 1). Afterwards, four …

Deviations Of Feedstuffs Loading In Tmr Preparation, Seli̇m Sirakaya, Osman Küçük

Turkish Journal of Veterinary & Animal Sciences

Loading of feedstuffs in planned amounts in total mixed ration (TMR) recipes are very important in terms of profitability, productivity, animal health, and performance. In the scope of this study, the planned and loaded feedstuffs amounts were determined in the mixer wagons. The study was conducted on a dairy cattle farm with ~1500 milking cow capacity. In this study, 13,276 feedstuffs loading data were examined and the mean and standard deviation of the deviation from the planned amount was 8.95 ± 18.38%. Deviation values of feedstuffs loading were found to be different; according to 1- TMR preparing operators, 2- type …

Sybr Green-Based Real-Time Pcr Detection Of Canine Babesia Spp. In Ixodid Ticks Infesting Dogs In Kerala, South India, Lavinia Wahlang, Bindu Lakshmanan, Naicy Thomas, Anu Bosewell, Jain Jose, Sunanda Chulliyil, Aravindakshan Thazhathuveetil

Turkish Journal of Veterinary & Animal Sciences

Ticks, being hematophagous ectoparasites, are notorious vectors that transmit an array of pathogenic viruses, bacteria, and protozoa while simultaneously exhibiting a zoonotic potential by transmitting pathogens that affect the health of owners in contact. The distribution of ixodid ticks of the genera Rhipicephalus and Haemaphysalis spp. in tropical climate of the state contributes to many serious tick-borne parasitic and rickettsial infections in domestic and wild canines. In south India, canine babesiosis is one of the most prevalent vector-borne parasitic diseases. Detection of parasites in tick vectors has significant epidemiological implications. The present study was designed to identify the presence of …

Evaluation Of Antioxidant Activity Of Bee Products Of Different Bee Races In Turkey, Özlem Saral, Mehmet Kiliçarslan, Hüseyi̇n Şahi̇n, Oktay Yildiz, Barbaros Di̇nçer

Turkish Journal of Veterinary & Animal Sciences

Bee products with high bioactive properties have a very important place in human nutrition. Biochemical properties of these products such as honey, pollen, and propolis vary according to flora, season, bee race, and production methods. In this study, the biological activities of honey, bee pollen, and propolis samples from four bee races in Turkey (Apis mellifera caucasica, Apis mellifera anatoliaca, Apis mellifera syriaca, and Apis mellifera carnica) were examined. Besides the determining of total polyphenol and total flavonoid contents, the antioxidant capacity was investigated by three different assays: DPPH (1,1-diphenyl-2-picrylhydrazyl) radical-scavenging activities, CUPRAC (cupric reducing antioxidant capacity) and FRAP (ferric …

Determination Of Antioxidants In Bovine Oviduct Epithelial Cell Culture Isolated At Different Periods Of The Estrous Cycle, Aytül Kürüm, Si̇yami̇ Karahan, Hakan Kocamiş, Mi̇yase Çinar, Emel Ergün

Turkish Journal of Veterinary & Animal Sciences

Oxidative stress interferes with oviduct functions including oocyte maturation, capacitation, fertilization, and embryo and gamete transport. This study aimed to determine activity of the antioxidants glutathione peroxidase (GPX-I), superoxide dismutase (SOD), and catalase (CAT) in bovine oviduct epithelial cells (BOEC) isolated from the isthmus and ampulla of the oviduct at estral (n = 7) and luteal phases (n = 7) of the estrous cycle. The antioxidant activity was measured at the primary, first, and second passages of the cell culture, and was characterized by cytokeratin expression. The GPX activity increased over the passages in samples of the ampulla and the …

The Effects Of Supplementation Of Rumen-Protected Choline On Some Blood And Milk Metabolites In The Transition Period Of Dairy Cattle, Ni̇yazi̇ Alan, Mustafa Salman

Turkish Journal of Veterinary & Animal Sciences

The aim of this study was to investigate nonesterified fatty acid (NEFA), beta-hydroxy butyric acid (BHBA), and milk urea nitrogen (MUN) profiles for dairy cows fed with rumen-protected choline (RPC) during the transition period. A total of 30 dairy cows were allocated to one control and two experimental groups (10 animals per group) and were fed from 3 weeks before calving to 21 days in milk. The control was fed only the basal diet. Treatment groups 1 and 2 were fed a basal diet containing 60 and 120 g/head/day RPC, respectively. The differences between groups were insignificant for MUN levels …

Serodiagnostic Potential Of Brucella Outer Membrane And Periplasmic Proteins, Aitbay Bulashev, Orken Akibekov, Zhanbolat Suranshiyev, Bakytkali Ingirbay, Saule Eskendirova

Turkish Journal of Veterinary & Animal Sciences

The aim of this study was to evaluate the serological diagnostic potential of the Brucella recombinant outer membrane (rOMP25, rOMP31) and periplasmic proteins (rBP26, rSOD) in a comparative way using an indirect enzyme-linked immunosorbent assay (i-ELISA). Rabbit and/or mouse antibodies to Brucella whole cell and/or soluble protein preparations recognized all recombinant proteins used, which confirms the expression of target antigens in E. coli in active form. The recombinant proteins showed different antigenicity to antibodies of cattle kept on a brucellosis-affected (endemic) farm and/or a new focus of infection. Thus, the presence of anti-Brucella antibodies was confirmed by i-ELISA/rSOD in 79% …

Repeated Low Doses Of Dexamethasone As A Pretreatment For Induction Of Parturition In Simmental Cows, Seçki̇n Salar, Hali̇t Kanca, Fi̇li̇z Bakar, Ayhan Baştan

Turkish Journal of Veterinary & Animal Sciences

The aim herein was to evaluate the effects of the induction of parturition using repeated low doses of dexamethasone in cattle. A total of 28 Simmental cows were used for the induction of parturition (n = 20) or served as controls (n = 8). Parturition was induced by a 1.6-mg intramuscular (IM) injection of dexamethasone twice daily for 6 days, beginning on day 270, and a 40-mg IM injection of dexamethasone on day 276. The calf birth weight, incidences of dystocia, daytime parturitions, and placental retention were compared. The calf birth weights in the treatment and the control groups were …

A Study On Oocyte Development Of The Atlantic Bluefin Tuna(Thunnus Thynnus, Linnaeus 1758) Under Farming Conditions, Şükrü Yildirim, Sema Mi̇di̇lli̇, Mehmet Güler, Ridvan Cenk Yurttaş, Deni̇z Çoban

Turkish Journal of Veterinary & Animal Sciences

Body length and weight measurements and histological samples were collected from slaughtered Atlantic bluefin tuna (Thunnus thynnus) that were fattening under farming conditions for the determination of oocyte development in February 2015 (n = 10), May 2015 (n = 1), June 2015 (n = 1), July 2015 (n = 9), and July 2016 (n = 3) during harvest and soon after newly dead. The developmental phases of oocytes were evaluated based on their diameters, fork body length and weight of fish, surface water temperature, and species' reproduction season. According to the histological results, the oocytes were at the beginning of …