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2019

Ecology

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Articles 31 - 60 of 65

Full-Text Articles in Life Sciences

Assessing Populations Of Eastern Brook Trout (Salvelinus Fontinalis) Above And Below Waterfalls In Mountain Streams Of Virginia, Hannah Eisemann Macmillan May 2019

Assessing Populations Of Eastern Brook Trout (Salvelinus Fontinalis) Above And Below Waterfalls In Mountain Streams Of Virginia, Hannah Eisemann Macmillan

Masters Theses, 2010-2019

Anthropogenically driven factors, such as increasing temperature and sediment in valley streams, acidification of mountain streams, and the introduction of non-native trout, are restricting habitat suitable for healthy populations of eastern brook trout (Salvelinus fontinalis) throughout their native Appalachian range. Brook trout are important as predators of insects in mountain streams and as a favorite of anglers. It is crucial that remaining populations in sustainable habitats be identified and preserved. Waterfalls are geologic knickpoints preventing base-level lowering that create unique, stable landscapes above them, which may alleviate the temperature-productivity/acidity “habitat squeeze” for populations of brook trout and could …


Insect Populations Across An Urban-To-Suburban Land Use Gradient., Jacob T. Lawson May 2019

Insect Populations Across An Urban-To-Suburban Land Use Gradient., Jacob T. Lawson

College of Arts & Sciences Senior Honors Theses

Insect populations, especially those of pollinators, have been steadily declining across the globe in recent decades, a trend that is intensified in cities. Since the conservation of pollinators is crucial for maintaining ecosystems and ecological processes, new approaches are being promoted beyond those of conserving large natural areas. Urban native plant gardens could potentially offset some of these losses locally. This research attempts to set a local baseline for the insect diversity in urban gardens in Louisville and determine whether differences exist in garden insects in cities versus suburbs in Jefferson County, Kentucky. To address the land-use question we established …


Presence And Distribution Of Leptodactylus Guianensis Heyer And De Sa, 2011 In Colombia: Comparisons With Other Species In The L. Latrans Group., Mariela Osorno Muñoz, Doris L. Gutiérrez-Lamus, Rafael O. De Sá Apr 2019

Presence And Distribution Of Leptodactylus Guianensis Heyer And De Sa, 2011 In Colombia: Comparisons With Other Species In The L. Latrans Group., Mariela Osorno Muñoz, Doris L. Gutiérrez-Lamus, Rafael O. De Sá

Biology Faculty Publications

Leptodactylus guianensis is reported for the first time for Colombia along with a distributional map of the species. The species is the fifth species of the L. latransspecies group documented in the country. Males possess a single, conical and slightly chisel-shaped thumb spine; only three species in the group have a single thumb spine. In collections, specimens of L. guianensis are misidentified as L. macrosternum, L. latrans or L. bolivianus. We provide morphological, colouration and habitat descriptions for where the species occurs in Colombia.


Agricultural Intensification And Urbanization Negatively Impact Soil Nematode Richness And Abundance: A Meta-Analysis, Satyendra K. Pothula, Parwinder Grewal, Robert M. Auge, Arnold M. Saxton, Ernest C. Bernard Apr 2019

Agricultural Intensification And Urbanization Negatively Impact Soil Nematode Richness And Abundance: A Meta-Analysis, Satyendra K. Pothula, Parwinder Grewal, Robert M. Auge, Arnold M. Saxton, Ernest C. Bernard

School of Earth, Environmental, and Marine Sciences Faculty Publications and Presentations

Human activity has extensively transformed the land surface by agricultural intensification and urbanization. In soil, nematodes are the most abundant invertebrates. The effect of human interventions was assessed on overall richness, overall abundance, richness and abundance of nematodes of each trophic group and colonizer-persister (c-p) guild by comparing urban, agriculture and disturbed grassland (DGL) with natural grassland (NGL) and forest ecosystems. Meta-analyses were conducted to generate quantitative summaries from 111 published articles that met the inclusion criteria, 91 expressed data in grams and 20 expressed data in cm3. Results from data expressed per 100 g of soil indicated that overall …


Effects Of Amur Honeysuckle On Soil Co2 Emissions, Aidan Shumaker Apr 2019

Effects Of Amur Honeysuckle On Soil Co2 Emissions, Aidan Shumaker

Student Symposium

Lonicera maackii is an invasive woody species found in Ohio and throughout the deciduous forests of eastern United States, taking over habitats of native shrub species such as Lindera benzoin. L. maackii has unique traits compared to deciduous forest natives, showing earlier leaf-out and later leaf senescence, higher leaf nitrogen levels, and faster leaf decomposition. As a result, L. maackii may alter soil conditions through its extended root activity and impacts on microbial decomposition, possibly impacting soil CO2 emissions. As soil microorganisms metabolize organic matter, they release greenhouse gases like CO2 through respiration, which can impact global climate change. Previous …


Fish And Wildlife Conservation For Educators Nrs 580, Michael Cerbo Mar 2019

Fish And Wildlife Conservation For Educators Nrs 580, Michael Cerbo

Library Impact Statements

No abstract provided.


The Role Of Temperature In Affecting Carry-Over Effects And Larval Competition In The Globally Invasive Mosquito Aedes Albopictus, Nnaemeka F. Ezeakacha, Donald Yee Mar 2019

The Role Of Temperature In Affecting Carry-Over Effects And Larval Competition In The Globally Invasive Mosquito Aedes Albopictus, Nnaemeka F. Ezeakacha, Donald Yee

Faculty Publications

Background

Ectotherms, like mosquitoes, have evolved specific responses to variation in environmental conditions like temperature, and these responses could confer a fitness benefit or cost when carried-over to different life stages. However, effects of temperature on animals with complex life-cycles often only focus on part of their life-cycle, or only consider how single aspects of life-history may carry over to new stages. Herein we investigated how temperature affects intraspecific larval competition and carry-over effects from larval to adult stages in the widespread invasive Asian tiger mosquito Aedes albopictus.

Methods

For larval competition, larvae were reared at three densities (10, …


Spot-On! Exploring Comprehension Of Ecological Concepts With Abstract Art Analogies, Jane Huggins Mar 2019

Spot-On! Exploring Comprehension Of Ecological Concepts With Abstract Art Analogies, Jane Huggins

The STEAM Journal

Students enrolled in an online general studies course [GNM 2190: Extinct and Threatened Life] at Stockton University were presented with two different pieces of abstract art. They were asked to draw analogies from each piece of art to the ecological concepts studied in the course. Students provided written responses which were submitted as assignments in the learning management software, Blackboard [Bb]. This exercise was described as ‘open-ended’ by the instructor; no grading rubrics were used. Responses to each piece of art were collected from 47 students and were subsequently uploaded into Quirkosä software for qualitative analysis. Codes representing major concepts …


Creative Citizen Science Illuminates Complex Ecological Responses To Climate Change, Abraham J. Miller-Rushing, Amanda S. Gallinat, Richard B. Primack Jan 2019

Creative Citizen Science Illuminates Complex Ecological Responses To Climate Change, Abraham J. Miller-Rushing, Amanda S. Gallinat, Richard B. Primack

Biology Faculty Publications

Climate change is causing the timing of key behaviors (i.e., phenology) to shift differently across trophic levels and among some interacting organisms (e.g., plants and pollinators, predators and prey), suggesting that interactions among species are being disrupted (1, 2). Studying the phenology of interactions, however, is difficult, which has limited researchers’ ability to zero in on changes in specific interactions or on the consequences of mismatches. In PNAS, Hassall et al. (3) use a combination of citizen science techniques to investigate the effects of climate change on dozens of specific interactions. They focus on a Batesian mimicry complex involving stinging …


Food Writing, Carol Ann Connare Ms Jan 2019

Food Writing, Carol Ann Connare Ms

Sustainability Education Resources

This advanced writing four-credit course approaches food writing from a news reporting perspective. The Pioneer Valley is home to a network of food producers, from farmers and cheesemakers to brewers and beekeepers. Students will travel into the field to meet people who make and grow what we eat, conducting interviews and collecting information to synthesize into multimedia stories for publication around themes such as health, history, travel, ecology, animal welfare, social change, nutrition, and home cooking. Students will experience the full spectrum of food writing—blogs, magazine articles, personal essays, reviews, recipes, social and cultural commentary—and create stories in a variety …


Betting & Hierarchy In Paleontology, Leonard Finkelman Jan 2019

Betting & Hierarchy In Paleontology, Leonard Finkelman

Faculty Publications

In his Rock, Bone, and Ruin: An Optimist’s Guide to the Historical Sciences, Adrian Currie argues that historical scientists should be optimistic about success in reconstructing the past on the basis of future research. This optimism follows in part from examples of success in paleontology. I argue that paleontologists’ success in these cases is underwritten by the hierarchical nature of biological information: extinct organisms have extant analogues at various levels of taxonomic, ecological, and physiological hierarchies, and paleontologists are adept at exploiting analogies within one informational hierarchy to infer information in another. On this account, fossils serve the role …


Management Recommendations For Ecological Restoration On The Little River Parcel, Maya Sady Partain, Garitt Mathews Jan 2019

Management Recommendations For Ecological Restoration On The Little River Parcel, Maya Sady Partain, Garitt Mathews

Environmental Science & Management Senior Capstones

Ecological restoration is an important aspect of land management. Data collection and ongoing monitoring help inform restoration decisions and determine their effectiveness. In this project, we followed a monitoring protocol provided by the Trinidad Coastal Land Trust to assess the 2019 status of their Little River parcel, a fifteen-acre area near the mouth of Little River in Trinidad, California. We performed a site analysis of invasive plant species and recorded their extent and location, as well as noting other observations of the ecology, topography, and biology of the site. After making our observations, we recommended land management methods woven together …


Ranging And Behavior Of Black And Gold Howler Monkeys In Formosa, Argentina, Claire Rossi De Leon Jan 2019

Ranging And Behavior Of Black And Gold Howler Monkeys In Formosa, Argentina, Claire Rossi De Leon

Library Map Prize

Howler monkeys (Alouatta spp.) are known for their adaptability, allowing them to inhabit a large range of forest types and driving high levels of variation in ranging and behavioral patterns. I address a series of hypotheses relating these relationships: 1) If an energy minimizing lifestyle is an adaptation to eating high quantities of leaves, then howler groups that have a high proportion of leaves in their diet will occupy smaller home ranges, have shorter daily path lengths, and spend more time resting, 2) if temperature is the primary driver of high levels of resting, then howlers will conserve energy and …


Functional Diversity And Restoration Of Meadows In Northeast Ohio, Keri Plevniak Jan 2019

Functional Diversity And Restoration Of Meadows In Northeast Ohio, Keri Plevniak

ETD Archive

Restoration outcomes can be variable and there is a need to understand the short- and long-term responses of the vegetation community. It is important to see if management goals are being met by evaluating restoration outcomes. Restoration goals often include establishing native species, increasing conservation value, and providing pollinator resources throughout the year. Assessing communities is commonly done though a taxonomic approach by using species presence and abundance. Another method of evaluation is through functional traits or species traits and their abundance. Both give different perspectives on how the community is achieving management goals. While taxonomic assessments can give insight …


[Introduction To] Collateral Values: The Natural Capital Created By Landscapes Of War., Todd R. Lookingbill, Peter D. Smallwood Jan 2019

[Introduction To] Collateral Values: The Natural Capital Created By Landscapes Of War., Todd R. Lookingbill, Peter D. Smallwood

Bookshelf

This book explores the unanticipated benefits that may arise after wars and conflicts, showing how the preservation of battlefields and the establishment of borderlands can create natural capital in the former landscapes of war. The editors call this Collateral Value, in contrast to the collateral damage that war inflicts upon infrastructure, natural capital, and human capital. The book includes case studies recounting successes and failures, opportunities and risks, and ambitious proposals.

The book is organized in two sections. The first visits U.S., English, and French battlefield sites dating from medieval England to World War I. The second explores borderlands located …


Bacterioplankton Biogeography Of The Mississippi River Basin, Jason Taylor Payne Jan 2019

Bacterioplankton Biogeography Of The Mississippi River Basin, Jason Taylor Payne

Electronic Theses and Dissertations

Bacterioplankton are important structural and functional components of river networks yet their biogeographical patterns in these systems are largely unknown. Using Illumina sequencing of the 16S rRNA gene we characterized variation in bacterioplankton community alpha diversity (within-sample richness of operational taxonomic units OTUs) and beta diversity (between-sample differences in composition) (1) along a 1 300-km downstream reach of the Mississippi River (2) over a range of temporal scales in the Lower Mississippi River (LMR) and (3) along cross-sectional transects repeated monthly for six months across the LMR floodplain. Particle-associated assemblages were richer in bacterial OTUs and more productive than free-living …


Ecology, Monogamy, And The Evolution Of Animal Families, Jacqueline Rae Dillard Jan 2019

Ecology, Monogamy, And The Evolution Of Animal Families, Jacqueline Rae Dillard

Theses and Dissertations--Biology

Family-living has been recognized as a necessary prerequisite for the evolution of advanced cooperative societies, yet the evolutionary and ecological processes that drive the coupling of different forms of cooperation in family-based societies are still poorly understood. In my dissertation, I investigate the correlated evolution of parental care, monogamy, and cooperative breeding in a variety of family-based taxa. I explore the mating and social behavior of family-living beetles with incipient cooperation to better understand the factors driving these social traits. Specifically, I evaluate different causes of extra-pair mating in socially monogamous beetles, the potential benefits that young adult offspring may …


The Effects Of Temperature On Maternal Investment Of Ovary Tissue In The Fish Species Danio Rerio, Noah R. Dillon, Alyce Demarais Jan 2019

The Effects Of Temperature On Maternal Investment Of Ovary Tissue In The Fish Species Danio Rerio, Noah R. Dillon, Alyce Demarais

Summer Research

The impact of climate change is predicted to increase water temperatures by 2 °C on average globally within the next century. As ectotherms, fish metabolism is directly connected to ambient water temperature. With a shift in metabolism due to temperature change, physiological processes like maternal investment may be affected. This study is an ecological and developmental investigation of the impacts of shifting environmental temperature conditions on the reproductive traits of the fish species Danio rerio. This study investigated Danio rerio under different temperatures to measure maternal investment. Female Danio rerio were exposed to temperature treatments 20-30 °C. Ovary tissue …


Where Birds Chill: An Assessment Of The Habitat Preferences Of Birds Overwintering In Hudson Valley Forests, Elizabeth Claire Axley Jan 2019

Where Birds Chill: An Assessment Of The Habitat Preferences Of Birds Overwintering In Hudson Valley Forests, Elizabeth Claire Axley

Senior Projects Spring 2019

Many avian species overwinter in eastern North America; however, studies on bird populations are rarely undertaken during this critical survival time, and little is known as to their habitat preferences and foraging behavior. In this observational study, we performed a survey of birds overwintering in the Hudson Valley’s temperate, primarily-deciduous forests, assessing avian populations’ habitat preferences through the vegetative structural variables surrounding overwintering birds as they forage. Our results suggest that high canopy cover is critically important to predicting overwintering bird occupancy on a microhabitat scale. Moreover, overwintering birds preferentially occupy forest plots not dominated by sugar maples, in spite …


Identification Of Antibiotic Ge37468a From Pseudonocardia Symbionts Of Trachymyrmex Septentrionalis Ants, Krithika Rao Jan 2019

Identification Of Antibiotic Ge37468a From Pseudonocardia Symbionts Of Trachymyrmex Septentrionalis Ants, Krithika Rao

Scripps Senior Theses

In response to the growing rates of antibiotic resistance in human bacterial pathogens, this study explores the natural products involved in the defensive symbiosis between actinobacteria and fungus-growing ants to uncover new potential antibiotics. This study also seeks to understand the function of natural antibiotics in their ecological contexts, especially those involved in defensive symbioses. Defensive symbiosis can be a beneficial platform for discovering useful antibiotics, because antibiotics in these relationships must be able to selectively inhibit enemies without harming hosts, and are therefore likely more specific and less toxic. Pseudonocardia sp. associated with Trachymyrmex septentrionalis ants demonstrated antibiotic activity …


City Limits: Heat Tolerance Is Influenced By Body Size And Hydration State In An Urban Ant Community, Dustin Jerald Johnson Jan 2019

City Limits: Heat Tolerance Is Influenced By Body Size And Hydration State In An Urban Ant Community, Dustin Jerald Johnson

University of the Pacific Theses and Dissertations

Cities are rapidly expanding, and global warming is intensified in urban environments due to the urban heat island effect. Therefore, urban animals may be particularly susceptible to warming associated with ongoing climate change. Thus, I used a comparative and manipulative approach to test three related hypotheses about the determinants of heat tolerance or critical thermal maximum (CTmax) in urban ants—specifically, that (1) body size, (2) hydration status, and (3) preferred micro-environments influence CTmax. I further tested a fourth hypothesis that native species are particularly physiologically vulnerable in urban environments. I manipulated water access and determined CTmax for 11 species common …


Plant Community Response To Novel Silvicultural Treatments In Great Lakes Northern Hardwoods, Stefan F. Hupperts Jan 2019

Plant Community Response To Novel Silvicultural Treatments In Great Lakes Northern Hardwoods, Stefan F. Hupperts

Dissertations, Master's Theses and Master's Reports

The objective of this dissertation is to assess plant community response across a range of silvicultural disturbances and test ecological hypotheses to better inform ecologists and forest managers. To provide context for the utility of revising silvicultural systems, I review natural disturbance regimes and historical practices that have shaped contemporary Great Lakes northern hardwood forests (Chapter 2). Further, I identify important ways to expand the silvicultural toolbox and better emulate natural disturbance regimes. Building on this theoretical underpinning, I investigate the initial regeneration and plant community response to two novel silvicultural experiments: the Northern Hardwood Experiment for Enhancing Diversity (NHSEED) …


Nature's Queer Negativity: Between Barad And Deleuze, Steven Swarbrick Jan 2019

Nature's Queer Negativity: Between Barad And Deleuze, Steven Swarbrick

Publications and Research

This essay offers a critique of the vitalist turn in queer and ecological theory, here represented by the work of Karen Barad. Whereas Barad advances an image of life geared towards meaningful connection with others, human and nonhuman, Deleuze advances an a-signifying ontology of self-dismissal. The point of this essay isn’t to separate their two views, but to draw out the consequences of their entanglement. Insofar as Barad’s work conceptualizes life (and art) as a vitalizing encounter, it cannot, this essay argues, account for the queer negativity at play in environmental politics, including the politics of climate change.


Cognition, Emotion, Personality And The Conservation And Management Of Wild Ungulates, Rob Found Jan 2019

Cognition, Emotion, Personality And The Conservation And Management Of Wild Ungulates, Rob Found

Animal Sentience

Increasing public understanding of the complexity of wild ungulates can improve animal welfare and advance global conservation efforts of these keystone species. Unfortunately, shaping public opinion on wild species is challenging because personal experience with wildlife is declining, popular education is still biased towards the predator instead of the prey, and scientific research is more difficult to conduct on wild ungulates compared to those on farms, in zoos, or otherwise in captivity. Nevertheless, studies of cognition, individuality, and intelligence of wild ungulates are increasing. I briefly highlight some major results from my own work on complexity in wild elk, illustrating …


Competition Or Facilitation: Examination Of Interactions Between Endangered Sida Hermaphrodita And Invasive Phragmites Australis, Samantha N. Mulholland Jan 2019

Competition Or Facilitation: Examination Of Interactions Between Endangered Sida Hermaphrodita And Invasive Phragmites Australis, Samantha N. Mulholland

Theses and Dissertations (Comprehensive)

Virginia Mallow (Sida hermaphrodita) is a perennial herb of the Malvaceae family that is native to riparian habitats in northeastern North America. Throughout most of its geographical distribution however, it is considered threatened and only two populations are known from Canada. The biology and ecology of S. hermaphrodita are still poorly understood and although few studies have been performed to determine the factors that contribute to the species rarity, it is considered threatened potentially due to the loss of habitat caused by exotic European Common reed (Phragmites australis subsp. australis) invasion. Allelopathic and phytotoxic conditioning of …


Parasitic Dinoflagellate Hematodinium Perezi Prevalence In Larval And Juvenile Blue Crabs Callinectes Sapidus From Coastal Bays Of Virginia, H. J. Small, J. P. Huchin-Mian, K. S. Reece, K. M. Pagenkopp Lohan, Mark J. Butler Iv, J. D. Shields Jan 2019

Parasitic Dinoflagellate Hematodinium Perezi Prevalence In Larval And Juvenile Blue Crabs Callinectes Sapidus From Coastal Bays Of Virginia, H. J. Small, J. P. Huchin-Mian, K. S. Reece, K. M. Pagenkopp Lohan, Mark J. Butler Iv, J. D. Shields

Biological Sciences Faculty Publications

The parasitic dinoflagellate Hematodinium perezi infects the American blue crab Callinectes sapidus and other decapods along the Eastern seaboard and Gulf of Mexico coast of the USA. Large juvenile and adult blue crabs experience high mortality during seasonal outbreaks of H. perezi, but less is known about its presence in the early life history stages of this host. We determined the prevalence of H. perezi in megalopae and early benthic juvenile crabs from multiple locations along the Virginia portion of the Delmarva Peninsula. The DNA of H. perezi was not detected in any megalopae collected from several locations within …


Role Of Managed Marine Areas On The Diversity And Individual Responses Of Rocky Intertidal Shore Grazers In Central Chile, Kathy Liu Jan 2019

Role Of Managed Marine Areas On The Diversity And Individual Responses Of Rocky Intertidal Shore Grazers In Central Chile, Kathy Liu

Scripps Senior Theses

Many different types of marine benthic herbivores or “grazers” inhabit coastal intertidal zones and play a crucial role in inter- and shallow subtidal ecosystems. Chile has one of the most diverse intertidal zones, but many intertidal grazers are exploited for human consumption. Marine protected areas (MPAs) and marine management and exploitation areas (MEAs) are promising tools for Chile to combat over exploitation of these grazer and other marine resources. This study surveyed the impact of sites with contrasting management on the diversity and abundance of all intertidal grazers and their impact on the size frequency and shell length-body weight allometry …


Distribution, Habitat, And Life History Aspects Of The Dwarf Crayfishes Of The Genus Cambarellus (Decapoda: Cambaridae) In Arkansas, Henry W. Robison, Chris T. Mcallister Jan 2019

Distribution, Habitat, And Life History Aspects Of The Dwarf Crayfishes Of The Genus Cambarellus (Decapoda: Cambaridae) In Arkansas, Henry W. Robison, Chris T. Mcallister

Journal of the Arkansas Academy of Science

The dwarf crayfishes of the genus Cambarellus are represented in Arkansas by only 2 species: Cambarellus (Pandicambarus) puer (Hobbs) and C. (P.) shufeldtii (Faxon). Both species are quite small and uncommonly encountered in the state. Between 1972 and 2018, we made 368 crayfish collections throughout the 75 counties of Arkansas. A total of 34 collections (our collections, plus museum specimens), and those previously collected by Reimer (1963) yielded a total of 304 specimens of C. puer and 12 collections of C. shufeldtii returned 54 specimens of C. shufeldtiii. Herein, we document these …


Long-Tailed Weasel (Mustela Frenata) Distribution Survey In Arkansas: Challenges In Detecting A Rare Species, Suzanne D. Johnston, D. Blake Sasse, Robert E. Kissell Jr. Jan 2019

Long-Tailed Weasel (Mustela Frenata) Distribution Survey In Arkansas: Challenges In Detecting A Rare Species, Suzanne D. Johnston, D. Blake Sasse, Robert E. Kissell Jr.

Journal of the Arkansas Academy of Science

Long-tailed weasels (Mustela frenata) have one of the widest distributions of mustelids in the western hemisphere and were distributed across a majority of the American continents ranging from Canada through the contiguous United States, Mexico, and into northern South America. However, on a local scale they are considered uncommon and rare. We assessed the distribution of long-tailed weasels across Arkansas to determine occupancy in each ecoregion of Arkansas, and determined the detectability on two local, adjacent sites. No long-tailed weasels were detected within the ecoregions, but the species was detected with intensive sampling on one local site. It …


Examination Of Behavioral Lateralizations In Juvenile Tarantulas In The Presence Of Prey Odors, Matthew Angelosanto Jan 2019

Examination Of Behavioral Lateralizations In Juvenile Tarantulas In The Presence Of Prey Odors, Matthew Angelosanto

Senior Honors Theses and Projects

The functional and/or structural specialization of the left or right side of the brain (behavioral lateralization) has substantial impacts on the interactions an animal makes with other organisms and its surroundings. While this has been extensively shown throughout vertebrates, it has recently been demonstrated in various taxa of invertebrates. Despite this, its presence in arachnids, and specifically tarantulas, remains poorly understood. The purpose of this study was to determine whether behavioral lateralization is present in the juvenile tarantulas Pterinochilus murinus and Brachypelma albopilosum in response to prey odors. If behavioral lateralization was found, I aimed to detennine how it differed …