Life Sciences Commons^™

Kinetics Of The Chromosome 14 Microrna Cluster Ortholog And Its Potential Role During Placental Development In The Pregnant Mare, Pouya Dini, Peter Daels, Shavahn C. Loux, Alejandro Esteller-Vico, Mariano Carossino, Kirsten E. Scoggin, Barry A. Ball

Maxwell H. Gluck Equine Research Center Faculty Publications

Background: The human chromosome 14 microRNA cluster (C14MC) is a conserved microRNA (miRNA) cluster across eutherian mammals, reported to play an important role in placental development. However, the expression kinetics and function of this cluster in the mammalian placenta are poorly understood. Here, we evaluated the expression kinetics of the equine C24MC, ortholog to the human C14MC, in the chorioallantoic membrane during the course of gestation.

Results: We demonstrated that C24MC-associated miRNAs presented a higher expression level during early stages of pregnancy, followed by a decline later in gestation. Evaluation of one member of C24MC (miR-409-3p) by in situ hybridization …

Does Persistent Snowpack Inhibit Degradation Of Fecal Stress Indicators?, Grace Parikh, Christopher R. Webster, John A. Vucetich, John J. Durocher, Joseph K. Bump

Michigan Tech Publications, Part 1

Physiological stress in wildlife can be a useful indicator of a population’s response to environmental factors. By using non-invasive endocrinological techniques, such as fecal sampling, potential confounding factors associated with the stress of capture can be avoided. A potential drawback of fecal sampling, however, is degradation of samples which may produce aberrant measurements of fecal glucocorticoid metabolites. In vertebrates, glucocorticoids, such as corticosterone, become elevated in response to stress. We sought to gauge the reliability of measurement of fecal glucocorticoid metabolites from white-tailed deer (Odocoileus virginianus) fecal samples exposed to a temperate winter with substantial snow cover and …

Legacy Habitat Suitability Of Eastern Oysters (Crassostrea Virginica) In Louisiana: A Prelude To Mississippi River Delta Freshwater Diversions, Tasia Mv Denapolis

LSU New Orleans Theses and Dissertations

The Louisiana oyster industry is greatly impacted by freshwater and sediment diversions that are part of the effort to restore the state’s coastline. A habitat suitability index (HSI) proposes species-habitat relationships that can be instrumental in creating impact assessments and suitability predictions for management as new diversions are implemented. An oyster (Crassostrea virginica) HSI was developed using three variables crucial to oyster sustainability: average annual salinity, minimum monthly salinity, and average salinity during the spawning season. These Legacy HSI visualizations show annual fluctuations in the distribution of zones suitable for oyster cultivation prior to proposed diversions in Pontchartrain …

A Change In Grain? Diet Induced Plasticity In The Generalist Grasshopper Melanoplus Differentialis, Austin M. Culotta

LSU New Orleans Theses and Dissertations

Phenotypic plasticity is favored in heterogeneous environments in which alternative phenotypes can exploit alternative resources. However, it’s not clear whether phenotypic plasticity is useful in environments that become more homogenous over an organism’s life cycle. I studied a population of grasshopper Melanoplus differentialis that experiences high resource diversity as nymphs but low resource diversity as adults to determine if individuals can undergo diet-induced morphological plasticity in head shape to increase biting ability and ingestion of hard diets. Insects on a soft diet were larger and had greater bite force than those on a hard diet. Head structures related to chewing …

Resveratrol Attenuates Allergic Asthma And Associated Inflammation In The Lungs Through Regulation Of Mirna-34a That Targets Foxp3 In Mice, Esraah Alharris, Hasan Alghetaa, Ratanesh K. Seth, Saurabh Chatterjee, Narendra P. Singh, Mitzi Nagarkatti, Prakash Nagarkatti

Faculty Publications

Asthma is a chronic inflammatory disease of airways mediated by T-helper 2 (Th2) cells involving complex signaling pathways. Although resveratrol has previously been shown to attenuate allergic asthma, the role of miRNA in this process has not been studied. We investigated the effect of resveratrol on ovalbumin-induced experimental allergic asthma in mice. To that end, BALB/c mice were immunized with ovalbumin (OVA) intraperitoneally followed by oral gavage of vehicle (OVA-veh) or resveratrol (100 mg/kg body) (OVA-res). On day 7, the experimental groups received intranasal challenge of OVA followed by 7 days of additional oral gavage of vehicle or resveratrol. At …

Bio Buzz Newsletter, Issue 16, December 2018, Department Of Biology

Biology Buzz Newsletter

No abstract provided.

Power In Pairs: Assessing The Statistical Value Of Paired Samples In Tests For Differential Expression, John R. Stevens, Jennifer S. Herrick, Roger K. Wolff, Martha L. Slattery

Mathematics and Statistics Faculty Publications

Background: When genomics researchers design a high-throughput study to test for differential expression, some biological systems and research questions provide opportunities to use paired samples from subjects, and researchers can plan for a certain proportion of subjects to have paired samples. We consider the effect of this paired samples proportion on the statistical power of the study, using characteristics of both count (RNA-Seq) and continuous (microarray) expression data from a colorectal cancer study.

Results: We demonstrate that a higher proportion of subjects with paired samples yields higher statistical power, for various total numbers of samples, and for various strengths of …

Understanding The Impacts Of Current And Future Environmental Variation On Central African Amphibian Biodiversity, Courtney A. Miller

LSU New Orleans Theses and Dissertations

Global climate change is projected to impact multiple levels of biodiversity by imposing strong selection pressures on existing populations, triggering shifts in species distributions, and reorganizing entire communities. The Lower Guineo-Congolian region in central Africa, a reservoir for amphibian diversity, is predicted to be severely affected by future climate change through rising temperatures and greater variability in rainfall. Geospatial modelling can be used to assess how environmental variation shapes patterns of biological variation – from the genomic to the community level – and use these associations to predict patterns of biological change across space and time. The overall goal of …

The Effect Of Styrene-Maleic Acid (Sma) Copolymers On Solubilizing Lipid Bilayers And Forming Nanodiscs, Ghada Alramadan

Dissertations and Theses

Cell membranes, or plasma membranes, play an essential role in the structure and the function of living cells. In 1972, the fluid mosaic membrane model was the first unifying paradigm of membrane structure. It is no longer considered adequate because evidence of many non-homogeneous lipid structures in both natural and model membranes have been discovered over the past thirty years. The field of membrane biophysics now uses updated versions of the mosaic model, which consists of the complex mixture of different lipid species. The lipid species found in natural membranes produce a range of dynamic, laterally segregated, non-homogeneous domains, which …

Does The Genomic Landscape Of Species Divergence In Phaseolus Beans Coerce Parallel Signatures Of Adaptation And Domestication?, Andrés J. Cortés, Paola Skeen, Matthew W. Blair, María I. Chacón-Sánchez

Agricultural and Environmental Sciences Faculty Research

Exploring the genomic architecture of species and populations divergence aids understanding how lineages evolve and adapt, and ultimately can show the repeatability of evolutionary processes. Yet, the genomic signatures associated with divergence are still relatively unexplored, leading to a knowledge gap on whether species divergence ultimately differs in its genetic architecture from divergence at other spatial scales (i.e., populations, ecotypes). Our goal in this research was to determine whether genomic islands of speciation are more prone to harbor within-species differentiation due to genomic features, suppressed recombination, smaller effective population size or increased drift, across repeated hierarchically nested levels of divergence. …

Within-Canopy Experimental Leaf Warming Induces Photosynthetic Decline Instead Of Acclimation In Two Northern Hardwood Species, Kelsey R. Carter, Molly A. Cavaleri

College of Forest Resources and Environmental Science Publications

Northern hardwood forests are experiencing higher temperatures and more extreme heat waves, potentially altering plant physiological processes. We implemented in-situ leaf-level warming along a vertical gradient within a mature forest canopy to investigate photosynthetic acclimation potential of two northern hardwood species, Acer saccharum and Tilia americana. After 7 days of +3°C warming, photosynthetic acclimation was assessed by measuring differences between heated and control photosynthetic rates (A_opt) at leaf optimum temperatures (T_opt). We also measured the effects of warming and height on maximum rates of Rubisco carboxylation, stomatal conductance, transpiration, and leaf traits: leaf …

Structured Diet And Exercise Guidance In Pregnancy To Improve Health In Women And Their Offspring: Study Protocol For The Be Healthy In Pregnancy (Bhip) Randomized Controlled Trial, Maude Perreault, Stephanie A. Atkinson, Michelle F. Mottola, Stuart M. Phillips, Keyna Bracken, Eileen K. Hutton, Feng Xie, David Meyre, Rita E. Morassut, Harry Prapavessis, Lehana Thabane

Anatomy and Cell Biology Publications

BackgroundEvidence from epidemiological and animal studies support the concept of programming fetal, neonatal, and adult health in response to in utero exposures such as maternal obesity and lifestyle variables. Excess gestational weight gain (GWG), maternal physical activity, and sub-optimal and excess nutrition during pregnancy may program the offspring's risk of obesity. Maternal intake of dairy foods rich in high-quality proteins, calcium, and vitamin D may influence later bone health status. Current clinical practice guidelines for managing GWG are not founded on randomized trials and lack specific active intervention ingredients. The Be Healthy in Pregnancy (BHIP) study is a randomized controlled …

2012 - Historic American Landscapes Survey - Mission San Antonio De Padua

Miscellaneous Monterey and San Luis Obispo County Documents and Reports

The intent of this report was to summarize existing written histories of the site and to document the existing conditions of the physical and cultural landscape at the Mission, This report focused on the viewshed and overall physical and cultural landscape of Mission San Antonio de Padua and its surroundings. There are no drawings of the site included in this documentation nor was the precise location and species of each plant type within the study area have not been documented or recorded. The study area encompasses approximately 650 acres.

2006 - Salinas Valley Integrated Regional Water Management Functionally Equivalent Plan Summary Document Update

Miscellaneous Monterey and San Luis Obispo County Documents and Reports

The California Department of Water Resources (DWR) and the State Water Resources Control Board (SWRCB) are encouraging local and regional water management planners to establish integrated regional water management plans through the collaboration of planning efforts and project coordination. The intent is to encourage planners to implement projects focused on meeting multiple water resources needs on a regional basis. Jointly, both agencies are soliciting grant applications for Proposition 50 Chapter 8 grant funding, which was established to provide a fiscal tool to support integrated regional water management. In order to take advantage of this funding opportunity, Monterey County Water Resources …

2010 - Excerpt From Master Response 4 - Water Supply Re Salinas Valley Water Supply (10-28-10)

Miscellaneous Monterey and San Luis Obispo County Documents and Reports

In addition to the Master Response to the Monterey County General Plan pertaining to the analysis of the Salinas Valley water supply, tables and figures referenced in the response are included at the end of the response. This document is for informational purposes only to provide a summary of information relative to the Salinas Valley water supply analysis contained in the Final EIR.

2008 - Managing An Uncertain Future - Climate Change Adaptation Strategies For California's Water

Miscellaneous Documents and Reports

This report recommends a number of adaptation strategies for California and local water managers to improve their capacity to handle change. Many of the strategies will also help adapt California's water resources to accommodate non-climate demands including a growing population, ecosystem restoration and greater flood protection. Several of the recommendations in this report are ready for immediate adoption, while others need additional public deliberation and development. Some can be implemented using existing resources and authority, while the majority will require new resources, sustained financial investment and significant collaborative effort.

Six Key Components Of A Farm Or Ranch Business Plan, Jay Parsons

Cornhusker Economics

Developing a good farm or ranch business plan can have many benefits. In an earlier Cornhusker Economics article, I outlined six good reasons to develop a business plan (Parsons 2015). It helps to get your business organized and moving in the right direction. It lets your lender know you have a plan to succeed, which opens up your access to capital. It also helps you organize your thoughts, clarifying the goals and objectives you wish to achieve. In summary, putting together a written business plan increases the likelihood of your business achieving success (Scarborough 2011).

The Catalytic Mechanism Of Electron-Bifurcating Electron Transfer Flavoproteins (Etfs) Involves An Intermediary Complex With Nad+, Gerrit J. Schut, Nishya Mohamed-Raseek, Monika Tokmina-Lukaszewska, David W. Mulder, Diep M. N. Nguyen, Gina L. Lipscomb, John Patrick Hoben, Angela Patterson, Carolyn E. Lubner, Paul W. King, John W. Peters, Brian Bothner, Anne-Frances Miller, Michael W. W. Adams

Chemistry Faculty Publications

Electron bifurcation plays a key role in anaerobic energy metabolism, but it is a relatively new discovery, and only limited mechanistic information is available on the diverse enzymes that employ it. Herein, we focused on the bifurcating electron transfer flavoprotein (ETF) from the hyperthermophilic archaeon Pyrobaculum aerophilum. The EtfABCX enzyme complex couples NADH oxidation to the endergonic reduction of ferredoxin and exergonic reduction of menaquinone. We developed a model for the enzyme structure by using nondenaturing MS, cross-linking, and homology modeling in which EtfA, -B, and -C each contained FAD, whereas EtfX contained two [4Fe-4S] clusters. On the basis …

Large-Scale Genome-Wide Meta-Analysis Of Polycystic Ovary Syndrome Suggests Shared Genetic Architecture For Different Diagnosis Criteria, Felix Day, Tugce Karaderi, Michelle R. Jones, Cindy Meun, Chunyan He, Alex Drong, Peter Kraft, Nan Lin, Hongyan Huang, Linda Broer, Reedik Magi, Richa Saxena, Triin Laisk, Margrit Urbanek, M. Geoffrey Hayes, Gudmar Thorleifsson, Juan Fernandez-Tajes, Anubha Mahajan, Benjamin H. Mullin, Bronwyn G. A. Stuckey, Timothy D. Spector, Scott G. Wilson, Mark O. Goodarzi, Lea Davis, Barbara Obermayer-Pietsch, André G. Uitterlinden, Verneri Anttila, Benjamin M. Neale, Marjo-Riitta Jarvelin, Bart Fauser

Internal Medicine Faculty Publications

Polycystic ovary syndrome (PCOS) is a disorder characterized by hyperandrogenism, ovulatory dysfunction and polycystic ovarian morphology. Affected women frequently have metabolic disturbances including insulin resistance and dysregulation of glucose homeostasis. PCOS is diagnosed with two different sets of diagnostic criteria, resulting in a phenotypic spectrum of PCOS cases. The genetic similarities between cases diagnosed based on the two criteria have been largely unknown. Previous studies in Chinese and European subjects have identified 16 loci associated with risk of PCOS. We report a fixed-effect, inverse-weighted-variance meta-analysis from 10,074 PCOS cases and 103,164 controls of European ancestry and characterisation of PCOS related …

Dichotomous Scoring Of Tdp-43 Proteinopathy From Specific Brain Regions In 27 Academic Research Centers: Associations With Alzheimer's Disease And Cerebrovascular Disease Pathologies, Yuriko Katsumata, David W. Fardo, Walter A. Kukull, Peter T. Nelson

Biostatistics Faculty Publications

TAR-DNA binding protein 43 (TDP-43) proteinopathy is a common brain pathology in elderly persons, but much remains to be learned about this high-morbidity condition. Published stage-based systems for operationalizing disease severity rely on the involvement (presence/absence) of pathology in specific anatomic regions. To examine the comorbidities associated with TDP-43 pathology in aged individuals, we studied data from the National Alzheimer’s Coordinating Center (NACC) Neuropathology Data Set. Data were analyzed from 929 included subjects with available TDP-43 pathology information, sourced from 27 different American Alzheimer’s Disease Centers (ADCs). Cases with relatively unusual diseases including autopsy-proven frontotemporal lobar degeneration (FTLD-TDP or FTLD-tau) …

Physicochemical And Ecotoxicological Assessment Of Biochar For Environmental Application, Maria Guillermina Cantou

LSU Doctoral Dissertations

Multiple benefits of biochar conversion and utilization, including soil carbon sequestration and environmental remediation, have been proposed by researchers. Biochar may be produced from a wide array of feedstocks and pyrolytic process conditions. This influences the final biochar composition, which includes the presence of potential pollutants that could ultimately result in detrimental environmental impacts. The major purpose of this research was to improve our understanding of potential unintended effects of biochar addition to the environment either as a potential source of toxicity towards organisms or as a material influencing availabilities of other soil contaminants. Biochars were produced from ten different …

2008 - San Antonio And Nacimiento Rivers Watershed Management Plan

Miscellaneous Monterey and San Luis Obispo County Documents and Reports

The purpose of the San Antonio and Nacimiento Rivers Watersheds Management Plan is to identify the existing conditions of and stresses in these watersheds as they relate to water quality, and recommend methods for reducing or eliminating those stressors such as alternative land use practices. The plan was created to:

􀂃 The reservoirs and their respective watersheds are the source for water recharging the Salinas Valley groundwater basin.

􀂃 There is a direct correlation between the health of these watersheds and the supply and quality of water for the Salinas Valley.

􀂃 The Salinas Valley’s population and economy are dependent …

2003 - Deep Aquifer Investigation - Hydrogeologic Data Inventory, Review, Interpretation And Implications

Miscellaneous Monterey and San Luis Obispo County Documents and Reports

The “deep aquifer” designation derives from the history of water resource development in Monterey County. Advancing seawater intrusion, first, in the 180-foot aquifer, and subsequently in the 400-foot aquifer, forced ground water users to progressively driller deeper to find fresh water. The first deep aquifer water well was drilled in 1976. In order to develop an improved understanding of the regional ground water resource, this technical memorandum attempts to integrate all available data on the aquifer systems underlying the 180- and 400-foot aquifers of the Salinas Valley. The available data set for the deep aquifer is scanty. These data are …

Parent And Household Influences On Calcium Intake Among Early Adolescents, Jinan Banna, Jessica O'Driscoll, Carol J. Boushey, Garry Auld, Beth Olson, Mary Cluskey, Miriam Edlefsen Ballejos, Christine Bruhn, Scottie Misner, Marla Reicks, Siew Sun Wong, Sahar Zaghloul

Nutrition, Dietetics, and Food Sciences Faculty Publications

Background: Calcium intake during early adolescence falls short of requirements for maximum bone accretion. Parents and the home food environment potentially influence children’s calcium intakes. This study aimed to quantify parental psychosocial factors (PSF) predicting calcium intakes of Asian, Hispanic, and non-Hispanic white (NHW) early adolescent children from a parental perspective.

Methods: This was a cross-sectional study involving the administration of a validated calcium-specific food frequency questionnaire to a convenience sample of children aged 10–13 years and the primary individual responsible for food acquisition in the child’s household. Based on Social Cognitive Theory, parental factors potentially associated with children’s calcium …

Salivary Α-Amylase Of Stem Borer Hosts Determines Host Recognition And Acceptance For Oviposition By Cotesia Spp. (Hymenoptera, Braconidae), Gladys B. Bichang'a, Jean Luc Da Lage, Kevin Sambai, Simon Mule, Bruno Le Ru, Laure Kaiser, Gerald Juma, Esther N. Maina, Paul André Calatayud

All Peer-Reviewed Publications

Foraging insect parasitoids use specific chemical cues to discriminate between host and non-host species. Several compounds have been identified in "host location and acceptance." However, nothing is known about the molecular variations in these compounds that could account for host-range differences between parasitoid species. In a previous study, it was shown that during the host-finding process, contact between the braconid Cotesia flavipes and its host is crucial, and that α-amylase of oral secretions from the host plays a key role for host acceptance and oviposition by the parasitoid. The present study sought to establish whether the variations in this enzyme …

Scorpions Of The Horn Of Africa (Arachnida, Scorpiones). Part Xvii.Revision Of Neobuthus, With Description Of Seven New Species From Ethiopia, Kenya And Somaliland (Buthidae), František Kovařík, Graeme Lowe, Ahmed Ibrahim Awale, Hassan Sh Abdirahman Elmi, Ali Abdi Hurre

Euscorpius

New information about the taxonomy and distribution of the genus Neobuthus Hirst, 1911 is presented, based on material recently collected mainly from Somaliland, but also Djibouti and Kenya. Emended diagnoses are proposed for N. berberensis Hirst, 1911 and N. ferrugineus (Kraepelin, 1898) sensu stricto. New species described are: N. amoudensis sp. n. from Ethiopia and Somaliland; N. erigavoensis sp. n., N. factorio sp. n., N. gubanensis sp. n., N. maidensis sp. n., and N. montanus sp. n. from Somaliland; and N. kloppersi sp. n. from Kenya. This doubles the number of species in Neobuthus, unearthing a rich diversification …

The Beetle Chronicler, Chris Dunker, Kayla Wolf, Brett Ratcliffe

University of Nebraska State Museum: Entomology Papers

Longtime UNL entomologist names beetles for dragons

Gymnetis rhaegali, Gymnetis drogoni, Gymnetis viserioni, Ambyoproctus boondocksius, Cyclocephala nadanotherwon, Strategus longichomperus

University of Nebraska entomology professor Brett Ratcliffe, who also curates the Nebraska State Museum's beetle collection, shows elephant beetles, a member of the scarab beetle family, in his office in Nebraska Hall.

Unlike their namesakes, three species of scarab beetles newly described by University of Nebraska-Lincoln entomologist Brett Ratcliffe do not breathe fire. Or, at least, entomologists and field researchers haven't observed them doing so.

Nor do the scarabs have the thick, reptilian scales and leathery wings like the trio of …

2016 - Memorandum Of Understanding For Integrated Regional Water Management In The Greater Monterey County Region With Amendments Through December 2016

Miscellaneous Monterey and San Luis Obispo County Documents and Reports

An Integrated Regional Water Management Plan (IRWM Plan) developed by 18 member entities that include government agencies, nonprofit organizations, educational organizations, water service districts, private water companies, and organizations representing agricultural, environmental, and community interests. The IRWM Plan is an expansion and modification of a previous plan – the May 2006 Salinas Valley Integrated Regional Water Management Functionally Equivalent Plan developed by Monterey County Water Resources Agency. This MOU recognizes the joint commitment of the undersigned parties to develop an IRWM Plan for the Greater Monterey County Region. The MOU does not impose any further commitments or obligations upon any …

High-Resolution Deconstruction Of Evolution Induced By Chemotherapy Treatments In Breast Cancer Xenografts., Hyunsoo Kim, Pooja A Kumar, Francesca Menghi, Javad Noorbakhsh, Eliza Cerveira, Mallory Ryan, Qihui Zhu, Guruprasad Ananda, Joshy George, Henry C Chen, Susan Mockus, Chengsheng Zhang, Yan Yang, James G. Keck, Radha Krishna Murthy Karuturi, Carol J Bult, Charles Lee, Edison Liu, Jeffrey H Chuang

Faculty Research 2018

The processes by which tumors evolve are essential to the efficacy of treatment, but quantitative understanding of intratumoral dynamics has been limited. Although intratumoral heterogeneity is common, quantification of evolution is difficult from clinical samples because treatment replicates cannot be performed and because matched serial samples are infrequently available. To circumvent these problems we derived and assayed large sets of human triple-negative breast cancer xenografts and cell cultures from two patients, including 86 xenografts from cyclophosphamide, doxorubicin, cisplatin, docetaxel, or vehicle treatment cohorts as well as 45 related cell cultures. We assayed these samples via exome-seq and/or high-resolution droplet digital …

Functional Outcomes In Early (T1/T2) Supraglottic Cancer: A Systematic Review, Benjamin Van Der Woerd, Krupal B. Patel, Anthony C. Nichols, Kevin Fung, John Yoo, Danielle S. Macneil

Anatomy and Cell Biology Publications

ObjectivesOrgan preserving surgery (OPS) and radiotherapy (RT) are both accepted treatment options for early stage supraglottic cancer (SGC). Radiation has supplanted surgery in most cases, because of the perception that surgery results in poorer functional outcomes. However, evidence suggests that OPS with a neck dissection may be associated with improved survival. Our objective was to conduct a systematic review of the literature to compare functional outcomes of OPS and RT for early SGC.MethodsWe searched Medline, EMBASE and Cochrane Central Register of Controlled Trials to identify studies. Studies were included if they reported functional outcomes on 10 or more patients with …