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Articles 1 - 23 of 23
Full-Text Articles in Life Sciences
The Toxicity Of Nanoparticles Depends On Multiple Molecular And Physicochemical Mechanisms, Yue-Wern Huang, Melissa Cambre, Han-Jung Lee
The Toxicity Of Nanoparticles Depends On Multiple Molecular And Physicochemical Mechanisms, Yue-Wern Huang, Melissa Cambre, Han-Jung Lee
Biological Sciences Faculty Research & Creative Works
Nanotechnology is an emerging discipline that studies matters at the nanoscale level. Eventually, the goal is to manipulate matters at the atomic level to serve mankind. One growing area in nanotechnology is biomedical applications, which involve disease management and the discovery of basic biological principles. In this review, we discuss characteristics of nanomaterials, with an emphasis on transition metal oxide nanoparticles that influence cytotoxicity. Identification of those properties may lead to the design of more efficient and safer nanosized products for various industrial purposes and provide guidance for assessment of human and environmental health risk. We then investigate biochemical and …
Accounting For Soil Inorganic Carbon In The Ecosystem Services Framework For United Nations Sustainable Development Goals, Garth Raymond Groshans
Accounting For Soil Inorganic Carbon In The Ecosystem Services Framework For United Nations Sustainable Development Goals, Garth Raymond Groshans
All Theses
Soil inorganic carbon (SIC) is currently not included in the list of key soil properties related to ecosystem services (e.g., provisioning, regulating, cultural, and supporting services). Soil inorganic carbon is a dynamic key soil property used in soil classification, taxonomy and fertility, therefore its inclusion in the framework of ecosystem services is important. With soils rapidly changing due to human use and climate change, the soil ecosystem services framework should not include only soil organic carbon (SOC), but SIC as well since it is of global importance to soil fertility and the long-term carbon cycle, especially in semiarid and arid …
Complement 3a Receptor In Dorsal Horn Microglia Mediates Pronociceptive Neuropeptide Signaling, Suzanne Doolen, Jennifer Cook, Maureen Riedl, Kelley Kitto, Shinichi Kohsaka, Christopher N. Honda, Carolyn A. Fairbanks, Bradley K. Taylor, Lucy Vulchanova
Complement 3a Receptor In Dorsal Horn Microglia Mediates Pronociceptive Neuropeptide Signaling, Suzanne Doolen, Jennifer Cook, Maureen Riedl, Kelley Kitto, Shinichi Kohsaka, Christopher N. Honda, Carolyn A. Fairbanks, Bradley K. Taylor, Lucy Vulchanova
Physiology Faculty Publications
The complement 3a receptor (C3aR1) participates in microglial signaling under pathological conditions and was recently shown to be activated by the neuropeptide TLQP‐21. We previously demonstrated that TLQP‐21 elicits hyperalgesia and contributes to nerve injury‐induced hypersensitivity through an unknown mechanism in the spinal cord. Here we determined that this mechanism requires C3aR1 and that microglia are the cellular target for TLQP‐21. We propose a novel neuroimmune signaling pathway involving TLQP‐21‐induced activation of microglial C3aR1 that then contributes to spinal neuroplasticity and neuropathic pain. This unique dual‐ligand activation of C3aR1 by a neuropeptide (TLQP‐21) and an immune mediator (C3a) represents a …
Novel Calcium-Related Targets Of Insulin In Hippocampal Neurons, Shaniya Maimaiti, Hilaree N. Frazier, Katie L. Anderson, Adam O. Ghoweri, Lawrence D. Brewer, Nada M. Porter, Olivier Thibault
Novel Calcium-Related Targets Of Insulin In Hippocampal Neurons, Shaniya Maimaiti, Hilaree N. Frazier, Katie L. Anderson, Adam O. Ghoweri, Lawrence D. Brewer, Nada M. Porter, Olivier Thibault
Pharmacology and Nutritional Sciences Faculty Publications
Both insulin signaling disruption and Ca2+ dysregulation are closely related to memory loss during aging and increase the vulnerability to Alzheimer's disease (AD). In hippocampal neurons, aging-related changes in calcium regulatory pathways have been shown to lead to higher intracellular calcium levels and an increase in the Ca2+-dependent afterhyperpolarization (AHP), which is associated with cognitive decline. Recent studies suggest that insulin reduces the Ca2+-dependent AHP. Given the sensitivity of neurons to insulin and evidence that brain insulin signaling is reduced with age, insulin-mediated alterations in calcium homeostasis may underlie the beneficial actions of insulin in …
Primary Cilium-Dependent Signaling Mechanisms, Rajasekharreddy Pala, Nedaa Alomari, Surya M. Nauli
Primary Cilium-Dependent Signaling Mechanisms, Rajasekharreddy Pala, Nedaa Alomari, Surya M. Nauli
Pharmacy Faculty Articles and Research
Primary cilia are hair-like organelles and play crucial roles in vertebrate development, organogenesis, health, and many genetic disorders. A primary cilium is a mechano-sensory organelle that responds to mechanical stimuli in the micro-environment. A cilium is also a chemosensor that senses chemical signals surrounding a cell. The overall function of a cilium is therefore to act as a communication hub to transfer extracellular signals into intracellular responses. Although intracellular calcium has been one of the most studied signaling messengers that transmit extracellular signals into the cells, calcium signaling by various ion channels remains a topic of interest in the field. …
Exopolysaccharides Regulate Calcium Flow In Cariogenic Biofilms, Monika Astasov-Frauenhoffer, Muth M. Varenganayil, Alan W. Decho, Tuomas Waltimo, Olivier Braissant
Exopolysaccharides Regulate Calcium Flow In Cariogenic Biofilms, Monika Astasov-Frauenhoffer, Muth M. Varenganayil, Alan W. Decho, Tuomas Waltimo, Olivier Braissant
Faculty Publications
Caries-associated biofilms induce loss of calcium from tooth surfaces in the presence of dietary carbohydrates. Exopolysaccharides (EPS) provide a matrix scaffold and an abundance of primary binding sites within biofilms. The role of EPS in binding calcium in cariogenic biofilms is only partially understood. Thus, the aim of the present study is to investigate the relationship between the calcium dissolution rates and calcium tolerance of caries-associated bacteria and yeast as well as to examine the properties of EPS to quantify its binding affinity for dissolved calcium. Calcium dissolution was measured by dissolution zones on Pikovskaya’s agar. Calcium tolerance was assessed …
Phylogenetic Patterns Of Foliar Mineral Nutrient Accumulation Among Gypsophiles And Their Relatives In The Chihuahuan Desert, Clare Muller, Michael J. Moore, Zoe Feder, Helene Tiley, Rebecca E. Drenovsky
Phylogenetic Patterns Of Foliar Mineral Nutrient Accumulation Among Gypsophiles And Their Relatives In The Chihuahuan Desert, Clare Muller, Michael J. Moore, Zoe Feder, Helene Tiley, Rebecca E. Drenovsky
2017 Faculty Bibliography
PREMISE OF THE STUDY: Gypsum endemism in plants (gypsophily) is common on gypsum outcrops worldwide, but little is known about the functional ecology of Chihuahuan Desert gypsophiles. We investigated whether leaf chemistry of gypsophile lineages from the northern Chihuahuan Desert are similar to leaves of related nonendemic (gypsovag) species relative to their soil chemistry. We expected widely distributed gypsophiles (hypothesized to be older lineages on gypsum) would have distinct leaf chemistry from narrowly distributed, relatively younger lineages endemic to gypsum and gypsovags, reflecting adaptation to gypsum.
METHODS: We collected leaves from 23 gypsophiles and related nonendemic taxa growing on nongypsum …
Calcium And Metabolism In Sperm: Fundamental Players For Fertilization And Embryo Development, Felipe Navarrete
Calcium And Metabolism In Sperm: Fundamental Players For Fertilization And Embryo Development, Felipe Navarrete
Doctoral Dissertations
Mammalian sperm acquire the fertilizing ability in the female tract in a process known as capacitation. At the molecular level, capacitation is associated with the up-regulation of a cAMP-dependent pathway, changes in intracellular pH, intracellular Ca2+, ATP consumption and an increase in tyrosine phosphorylation. However, little is known about the function of Ca2+ and ATP during this important process. These signaling systems interact during capacitation are still not well understood. Results presented in this study indicate that Ca2+ ions have a biphasic role in the regulation of cAMP-dependent signaling. Sperm incubated in zero Ca2+ in …
Effects Of Eggshell Removal On Embryonic Skeletal Development In The American Alligator (Alligator Mississippiensis), Nelson Armando Membreno
Effects Of Eggshell Removal On Embryonic Skeletal Development In The American Alligator (Alligator Mississippiensis), Nelson Armando Membreno
Electronic Theses, Projects, and Dissertations
The eggshell of reptiles is essential for not only protecting the embryo, but can also serve as source of calcium for embryonic skeletal development. Whereas embryonic lepidosaurs and chelonians rely on their yolk sac for calcium during development, embryonic archosaurs mobilise eggshell calcium supply to both the embryo and the yolk sac. By the time archosaurs hatch, their residual yolk sacs have a calcium content equal or greater than at time of oviposition, which is used to support post-hatching growth. To date, no study has looked into how removal of the calcareous eggshell affects embryonic development in archosaurs. I tested …
Mutant Tdp-43 Does Not Impair Mitochondrial Bioenergetics In Vitro And In Viv, Hibiki Kawamata, Pablo Peixoto, Csaba Konrad, Gloria Palomo, Kirsten Bredvik, Meri Gerges, Federica Valsecchi, Leonard Petrucelli, John M. Ravits, Anatoly Starkov, Giovanni Manfredi
Mutant Tdp-43 Does Not Impair Mitochondrial Bioenergetics In Vitro And In Viv, Hibiki Kawamata, Pablo Peixoto, Csaba Konrad, Gloria Palomo, Kirsten Bredvik, Meri Gerges, Federica Valsecchi, Leonard Petrucelli, John M. Ravits, Anatoly Starkov, Giovanni Manfredi
Publications and Research
Background: Mitochondrial dysfunction has been linked to the pathogenesis of amyotrophic lateral sclerosis (ALS) and frontotemporal lobar degeneration (FTLD). Functional studies of mitochondrial bioenergetics have focused mostly on superoxide dismutase 1 (SOD1) mutants, and showed that mutant human SOD1 impairs mitochondrial oxidative phosphorylation, calcium homeostasis, and dynamics. However, recent reports have indicated that alterations in transactivation response element DNA-binding protein 43 (TDP-43) can also lead to defects of mitochondrial morphology and dynamics. Furthermore, it was proposed that TDP-43 mutations cause oxidative phosphorylation impairment associated with respiratory chain defects and that these effects were caused by mitochondrial localization of the mutant …
The Effect Of Calcium In Artificial Substrates For Oyster Restoration: Implications For The Mitigation Of Oyster Population Decline, Jessie Mandirola
The Effect Of Calcium In Artificial Substrates For Oyster Restoration: Implications For The Mitigation Of Oyster Population Decline, Jessie Mandirola
Masters Theses, 2010-2019
Oyster populations in the Chesapeake Bay have declined by 99% over the past 150 years due to overharvesting, disease, ocean acidification, and poor water quality. Restoration efforts are needed to reestablish native oyster populations. Current restoration efforts utilize natural oyster shells but these methods are expensive and unsustainable. Therefore, restoration is starting to use artificial substrates instead. Concrete has been successfully used in previous research; spat will attach and oysters will grow. However, there is a lack of knowledge about how the composition of concrete effects oyster larval recruitment. We tested concrete made with limestone sand (“special concrete”) to increase …
Carisbamate Blockade Of T-Type Voltage-Gated Calcium Channels, Do Young Kim, Fang-Xiong Zhang, Stan T. Nakanishi, Timothy Mettler, Ik-Hyun Cho, Younghee Ahn, Florian Hiess, Lina Chen, Patrick G. Sullivan, S. R. Wayne Chen, Gerald W. Zamponi, Jong M. Rho
Carisbamate Blockade Of T-Type Voltage-Gated Calcium Channels, Do Young Kim, Fang-Xiong Zhang, Stan T. Nakanishi, Timothy Mettler, Ik-Hyun Cho, Younghee Ahn, Florian Hiess, Lina Chen, Patrick G. Sullivan, S. R. Wayne Chen, Gerald W. Zamponi, Jong M. Rho
Spinal Cord and Brain Injury Research Center Faculty Publications
Objectives
Carisbamate (CRS) is a novel monocarbamate compound that possesses antiseizure and neuroprotective properties. However, the mechanisms underlying these actions remain unclear. Here, we tested both direct and indirect effects of CRS on several cellular systems that regulate intracellular calcium concentration [Ca2+]i.
Methods
We used a combination of cellular electrophysiologic techniques, as well as cell viability, Store Overload‐Induced Calcium Release (SOICR), and mitochondrial functional assays to determine whether CRS might affect [Ca2+]i levels through actions on the endoplasmic reticulum (ER), mitochondria, and/or T‐type voltage‐gated Ca2+ channels.
Results
In CA3 pyramidal neurons, kainic …
Lactose Intolerance: An Overview Of The Facts And Their Implications, Noelle M. Yeo
Lactose Intolerance: An Overview Of The Facts And Their Implications, Noelle M. Yeo
Honors Theses
Lactose intolerance is often blamed for the symptoms such as bloating, diarrhea, gas, abdominal pain, and nausea, that ail many people. Patients often do not seek proper diagnosis from a physician and create their own treatment plans, severely restricting lactose intake, without professional guidance. Even those who do seek the care of a physician find that diagnosis is complicated by less-than ideal testing and confusion due to the symptoms common to many other conditions. The misconceptions and inability to confirm a diagnosis of lactose intolerance can cause nutrient deficiencies in these patients, as well as begin a pattern of unnecessary …
Calcium's Role As Nuanced Modulator Of Cellular Physiology In The Brain, Hilaree N. Frazier, Shaniya Maimaiti, Katie L. Anderson, Lawrence D. Brewer, John C. Gant, Nada M. Porter, Olivier Thibault
Calcium's Role As Nuanced Modulator Of Cellular Physiology In The Brain, Hilaree N. Frazier, Shaniya Maimaiti, Katie L. Anderson, Lawrence D. Brewer, John C. Gant, Nada M. Porter, Olivier Thibault
Pharmacology and Nutritional Sciences Faculty Publications
Neuroscientists studying normal brain aging, spinal cord injury, Alzheimer’s disease (AD) and other neurodegenerative diseases have focused considerable effort on carefully characterizing intracellular perturbations in calcium dynamics or levels. At the cellular level, calcium is known for controlling life and death and orchestrating most events in between. For many years, intracellular calcium has been recognized as an essential ion associated with nearly all cellular functions from cell growth to degeneration. Often the emphasis is on the negative impact of calcium dysregulation and the typical worse-case-scenario leading inevitably to cell death. However, even high amplitude calcium transients, when executed acutely can …
The Effect Of Site Characteristics On The Reproductive Output Of Lesser Celandine (Ranunculus Ficaria), Justin P. Kermack
The Effect Of Site Characteristics On The Reproductive Output Of Lesser Celandine (Ranunculus Ficaria), Justin P. Kermack
ETD Archive
Ranunculus ficaria L., an ephemeral perennial invasive plant brought over from Europe, is becoming widespread throughout the Northeastern United States. This herbaceous buttercup is able to create extensive dense mats that limit native species growth. Taking advantage of an early growing season and rapid reproduction rates, this species can create dense monocultures, which threatens native communities and ecosystems. Elimination of native spring ephemerals results in decreased biodiversity. A better understanding of how R. ficaria responds to site characteristics is needed to prioritize management efforts toward high-risk sites.
Ranunculus ficaria abundance and reproductive output (seed, bulbil and tuber production rates) were …
Feasibility Of Laser-Induced Breakdown Spectroscopy (Libs) As An At-Line Validation Tool For Calcium Determination In Infant Formula, Xavier Cama-Moncunill, Maria Markiewicz-Keszycka, Yash Dixit, Raquel Cama-Moncunill, Maria Piedad Casado-Gavalda, Patrick J. Cullen, Carl Sullivan
Feasibility Of Laser-Induced Breakdown Spectroscopy (Libs) As An At-Line Validation Tool For Calcium Determination In Infant Formula, Xavier Cama-Moncunill, Maria Markiewicz-Keszycka, Yash Dixit, Raquel Cama-Moncunill, Maria Piedad Casado-Gavalda, Patrick J. Cullen, Carl Sullivan
Articles
In this study, a 150 mJ laser-induced breakdown spectroscopy (LIBS) system was assessed to determine calcium content in infant formula (IF) samples. LIBS is a promising emission spectroscopic technique for elemental analysis, which offers advantages over conventional methods such as real-time analyses, little to no sample preparation and ease of use. The aim of this work was to evaluate the feasibility of LIBS as an at-line tool for IF manufacturing. To this end, IF mixtures with varying content of calcium were prepared over a range (approx. 1.5–7 mg/g of calcium) selected to be in conformity with the guidelines provided by …
A Presynaptic Role For Nitric Oxide At A Gabaergic Synapse, John Wesley Maddox
A Presynaptic Role For Nitric Oxide At A Gabaergic Synapse, John Wesley Maddox
LSU Doctoral Dissertations
Amacrine cells are a class of retinal interneurons that process the visual signal in the inner retina. Several subtypes of amacrine cells express nitric oxide synthase and produce nitric oxide (NO), making NO a possible regulator of amacrine cell function. My dissertation research tests the hypothesis that NO alters amacrine cell GABAergic synaptic output. To investigate this, I made whole-cell voltage clamp recordings of cultured chick amacrine cells receiving synaptic input from other amacrine cells and Ca2+ imaging of amacrine cell dendrites, which can be presynaptic. I find that NO-dependent increases in GABAergic spontaneous postsynaptic current (sPSC) frequency are …
Investigation Of The Regulation Mechanism Of Arabidopsis Thaliana Anion Channel Slah2, Fen-Yong Yao, Guo-Ning Qi, Jamshaid Husssain
Investigation Of The Regulation Mechanism Of Arabidopsis Thaliana Anion Channel Slah2, Fen-Yong Yao, Guo-Ning Qi, Jamshaid Husssain
Turkish Journal of Botany
In recent years a slow-type anion channel (SLAC) family composing five members, including slow anion channel associated 1 (SLAC1) and four SLAC1 homologs (SLAHs), has been identified in Arabidopsis thaliana. The anion channels are activated by calcium-dependent protein kinases (CPKs) and play crucial roles in mediating anion fluxes across plasma membranes. SLAH2 is specifically expressed in root stele cells and has been characterized as a nitrate-selective anion channel activated by CPK21 and CBL-interacting protein kinase 23 (CIPK23). However, the interaction between CPKs and SLAH2 and how it is regulated remains to be elucidated. In this study we found that SLAH2 …
A Natural, Calcium-Rich Marine Multi-Material Complex Preserves Bone Structure, Composition And Strength In An Ovariectomized Rat Model Of Osteoporosis, Orlaith Brennan, Joseph Sweeney, Brian O'Meara, Amro Widaa, Franck Bonnier, Hugh Byrne, Denis O'Gorman, Fergal O'Brien
A Natural, Calcium-Rich Marine Multi-Material Complex Preserves Bone Structure, Composition And Strength In An Ovariectomized Rat Model Of Osteoporosis, Orlaith Brennan, Joseph Sweeney, Brian O'Meara, Amro Widaa, Franck Bonnier, Hugh Byrne, Denis O'Gorman, Fergal O'Brien
Articles
Calcium supplements are used as an aid in the prevention of osteopenia and osteoporosis and also for the treatment of patients when used along with medication. Many of these supplements are calcium carbonate based. This study compared a calcium-rich, marine multi-mineral complex (Aquamin) to calcium carbonate in an ovariectomised rat model of osteoporosis in order to assess Aquamin’s efficacy in preventing the onset of bone loss. Animals were randomly assigned to either non-ovariectomy control (Control), ovariectomy (OVX) plus calcium carbonate, ovariectomy plus Aquamin or ovariectomy plus Aquamin delay where Aquamin treatment started 8 weeks post OVX. At the end of …
Assessment Of Sampling Technique From Feeders On Copper, Zinc, Calcium, And Phosphorus Analysis, A. M. Jones, J. C. Woodworth, C. Vahl, S. S. Dritz, M. D. Tokach, R. D. Goodband, J. M. Derouchey
Assessment Of Sampling Technique From Feeders On Copper, Zinc, Calcium, And Phosphorus Analysis, A. M. Jones, J. C. Woodworth, C. Vahl, S. S. Dritz, M. D. Tokach, R. D. Goodband, J. M. Derouchey
Kansas Agricultural Experiment Station Research Reports
While numerous research articles have been published on how to collect a representative sample, and analytical or laboratory-to-laboratory variation, we are unaware of any studies to examine exactly how many samples to collect from feeders, or whether they should be pooled or not to minimize analytical variation. Therefore, this study was designed to evaluate different sampling procedures and the number of samples to collect, and achieve an accurate assessment of nutrient fortification in swine diets.
Diet samples were collected from a study evaluating the effects of increasing Cu on growth performance of finishing pigs. Treatments were arranged in a split-plot …
N-Alkyl 4-Methylamphetamine Enantiomers And The Implication For Potential Modulation Of Abuse Liability And Enhancement Of Psychoactive Drug Targeting., Ramsey Sitta
Theses and Dissertations
Drugs of abuse have a long history in humanity. Currently however, a subject of great interest is the phenylalkylamine family of drugs. Not only is the abuse liability of interest but also the potential therapeutic expansion of the capabilities of this family of drugs by utilizing the unique stereospecific effects of the newly discovered hybrid compounds. Based upon prior data of N-Alkyl 4-MA the enantiomers of N-Methyl, N-Ethyl, and N-Propyl were analyzed in hDAT, hNET, and hSERT. It was found that there was a negative correlation between chain length and potency and dopaminergic component. In agreement with the currently established …
Effects Of Dietary Calcium And Phosphorus Concentrations And Addition Of Phytase On Growth Performance Of Nursery Pigs, F. Wu, M. D. Tokach, J. M. Derouchey, S. S. Dritz, J. C. Woodworth, R. D. Goodband
Effects Of Dietary Calcium And Phosphorus Concentrations And Addition Of Phytase On Growth Performance Of Nursery Pigs, F. Wu, M. D. Tokach, J. M. Derouchey, S. S. Dritz, J. C. Woodworth, R. D. Goodband
Kansas Agricultural Experiment Station Research Reports
A total of 720 nursery pigs (PIC 1050 × 280, initially 13.4 ± 0.47 lb) were used in a 42-d growth study to determine the effects of feeding 2 calcium (Ca) and 3 standardized total tract digestible (STTD) phosphorus (P) concentrations on growth performance and bone ash content. Pens of pigs (10 pigs/pen, 12 pens/treatment) were blocked by initial pen weight, and within blocks pens were allotted randomly to 1 of 6 dietary treatments. Dietary treatments were arranged in a 2 × 3 factorial, with 2 levels of Ca (0.58 vs. 1.03%) and 3 levels of STTD P (0.33 and …
Effects Of Dietary Ca And P Concentrations On Growth Performance Of 13- To 25-Lb Pigs, F. Wu, M. D. Tokach, J. M. Derouchey, S. S. Dritz, J. C. Woodworth, R. D. Goodband
Effects Of Dietary Ca And P Concentrations On Growth Performance Of 13- To 25-Lb Pigs, F. Wu, M. D. Tokach, J. M. Derouchey, S. S. Dritz, J. C. Woodworth, R. D. Goodband
Kansas Agricultural Experiment Station Research Reports
A total of 360 nursery pigs (PIC 1050 × 280, initially 13.3 ± 2.39 lb) were used in a 45-d growth study to determine the effects of feeding 2 standardized total tract digestible (STTD) P and 3 Ca concentrations on growth performance. In a completely randomized design, pens of pigs (10 pigs per pen, 6 pens per treatment) were allotted randomly to 1 of 6 dietary treatments. Dietary treatments were arranged in a 2 × 3 factorial, with 2 levels of STTD P (at or above NRC requirement estimates) and 3 levels of Ca (0.65, 0.90, and 1.20%). Diets formulated …