Life Sciences Commons^™

Alfalfa Response To Phosphorus And Potassium Fertility And Harvest Management, M. A. Islam, M. M. Baidoo

IGC Proceedings (1997-2023)

Balanced nutrition of phosphorus (P) and potassium (K) is needed for improving alfalfa (Medicago sativa L.) productivity. Availability and uptake of P and K by alfalfa for yield response can depend on their relative levels with soil calcium (Ca²⁺) and magnesium (Mg²⁺) in combination with harvest management. An experiment was conducted at the University of Wyoming Sustainable Agriculture Research and Extension Center to determine the alfalfa’s response to P and K in association with Ca, Mg, and harvest time. Treatments were 10 selected combinations (kg ha^-1) of three levels of P (0, 34, …

Understanding The Pizza Baking Properties Of Low Moisture Part Skim (Lmps) Mozzarella Cheese, Anjali Verma

All Graduate Theses and Dissertations, Fall 2023 to Present

Mozzarella cheese is expected to melt, stretch, release free oil and exhibit browning and blistering when baked on pizza. This research aimed to investigate the effects of different starter cultures (based on lactose and galactose utilizing ability) and calcium levels on the functionality of Mozzarella cheese when baked on pizza. Four combinations of starter cultures using S. thermophilus (STI 06)-control, Lac⁺ Gal⁺ L. helveticus (LH 32), and Lac^- Gal⁺ adjunct culture L. helveticus (LH 7995) were used to make the cheese samples. Various tests were conducted over a seven-week period to evaluate their baking properties. The …

Mineral Composition Of Serially Slaughtered Holstein Steers Supplemented With Zilpaterol Hydrochloride, Andrea K. Watson, Trent J. Mcevers, Lee-Anne J. Walter, Nathan D. May, Jacob A. Reed, J. C. Macdonald, Galen E. Erickson, N. Andy Cole, Johnjohn P. Hutcheson, Ty E. Lawrence

Department of Animal Science: Faculty Publications

Calf-fed Holstein steers (n = 115; 449 ± 20 kg) were utilized in a serial harvest experiment. A baseline group of five steers was harvested after 226 d on feed (DOF), which was designated day 0. The remaining cattle were assigned randomly to 11 harvest groups, with slaughter every 28 d. Cattle were either not (CON) or were fed zilpaterol hydrochloride for 20 d followed by a 3 d withdrawal (ZH). There were five steers per treatment in each slaughter group ranging from days 28 to 308. Whole carcasses were divided into lean, …

Qtl Analysis Of Mineral Content And Grass Tetany Potential In Leymus Wildryes, S. R. Larson, H. F. Mayland

IGC Proceedings (1997-2023)

Grass tetany is a metabolic ailment in ruminants, occurring when animals graze rapidly growing C₃ grasses with a K/(Mg+Ca) ratio (KRAT) greater than 2.2. High KRAT values have been documented in several forage grasses including diploid Russian wildrye (Jefferson et al., 2001). The objective of this experiment was to identify quantitative trait loci (QTLs) controlling KRAT in allotetraploid wildryes.

Kidney Stones And Energy Drinks: A Literature Review, Donovan D. Bentz

Schultz-Werth Award Papers

Kidney stones are common, painful, and expensive to treat. Several factors play a role in the development of stones, particularly the consumption of sugars such as fructose. As such, the consumption of sugary energy drinks contributes to the development of kidney stones. However, there is a lack of understanding behind the mechanism of kidney stone development and the role energy drinks play in stone development. This review analyzes the mechanism of kidney stone development and the effect energy drink consumption has on stone development. Here, we also detail the dietary roles calcium, oxalate, ascorbic acid, and animal protein have on …

Dataset For Manuscript: Comparing Performance Of Spectral Image Analysis Approaches For Detection Of Cellular Signals In Time-Lapse Hyperspectral Imaging Fluorescence Excitation-Scanning Microscopy, Silas J. Leavesley

BioImaging and BioSystems Research

The dataset contains raw and processed hyperspectral timelapse image data that are described in the manuscript:

Parker, M., Annamdevula, N. S., Pleshinger, D., Ijaz, Z., Jalkh, J., Penn, R., Deshpande, D., Rich, T. C. & Leavesley, S. J. Comparing Performance of Spectral Image Analysis Approaches for Detection of Cellular Signals in Time-Lapse Hyperspectral Imaging Fluorescence Excitation-Scanning Microscopy. Bioengineering 10, 642 (2023).

Calcium Imaging Of Central Amygdala Activity After Fentanyl Escalation, Samantha Malone

Theses and Dissertations--Psychology

Evidence suggests that rats given long access (LgA) sessions to self-administer (SA) opioids escalate their intake, while also showing greater withdrawal severity and drug-induced reinstatement compared to rats maintained on short access (ShA) daily SA sessions. Little is known about the neural changes that occur during opioid escalation that may impact withdrawal and relapse. Past work examining opioid SA using ShA sessions in rodents has identified the central amygdala (CeA) as an area of interest that becomes hyperactive in acute withdrawal and may be involved in the incubation of craving that occurs after protracted withdrawal. However, these studies have not …

Effects Of Feeding Finishing Pig Diets Differing In Ca:P Ratio, Added Phytase And Vitamin D Sources On Growth Performance, Weight Variation, Serum 25(Oh)D3, Carcass Characteristics, Bone Characteristics, And Economics, Hilario M. Cordoba, Jason C. Woodworth, Robert D. Goodband, Joel M. Derouchey, Mike D. Tokach, Jordan T. Gebhardt, Jon R. Bergstrom

Kansas Agricultural Experiment Station Research Reports

A total of 2,160 grow-finish pigs (PIC 337 × 1050; initially 71.8 ± 1.4 lb) were used in a 114-d trial to determine the effects of feeding diets differing in Ca:P ratio and added phytase or phytase and 25(OH)D₃ (Hy-D, DSM Nutritional Products, Parsippany, NJ) on growth performance, weight variation, serum 25(OH)D₃, bone characteristics, and economics. Pigs were housed in mixed gender pens with 27 pigs per pen and 20 pens per treatment. The four treatments were structured as a randomized complete block design and consisted of: 1) a high phosphorus (HP) diet formulated to a 1.25:1 …

Ankyrin Dependent Mitochondrial Function And Bioenergetics In The Heart, Janani Subramaniam, Janani Subramaniam

Dissertations & Theses (Open Access)

ANK2 mutations in patients are associated with numerous arrhythmias, cardiomyopathies, and other heart defects. In the heart, AnkB, the protein encoded by ANK2, clusters relevant ion channels and cell adhesion molecules in several important domains; however, its role at Mitochondria Associated ER/SR Membranes (MAMs) has yet to be investigated. MAMs are crucial to mitochondrial function and metabolism and are signaling hubs implicated in various cardiac pathologies. Among several functions, these sites mediate the direct transfer of calcium from the ER/SR to the mitochondria to modulate ATP synthesis. Given that mitochondrial function and energy production are paramount to cardiovascular heath, …

Recent Advancements On Calcium And Phosphorus Recommendations In Broilers, Justina Caldas, Marcelo Silva

Proceedings of the Arkansas Nutrition Conference

Calcium and phosphorus have been recognized essential nutrients in chickens since 1950’s (Lesson & Summers, 2001) playing important roles in bone development, among other functions. Calcium is assumed an inexpensive nutrient in animal least cost formulation while phosphorus is considered expensive and scant. The actual economic impact comes from the influence of calcium on phosphorus requirements, changes in phosphorus digestibility, buffer capacity, gut health, trace mineral absorption, fat digestibility, welfare traits, etc. Both minerals have important interactions to be considered during formulation. Few changes have been made over the past 20 years to calcium and phosphorus recommendations because these values …

Limestone Solubility: What Can You Do About It?, Wenting Li, Katie Hilton, Franco Mussini, Janet Remus

Proceedings of the Arkansas Nutrition Conference

Calcium (Ca) is essential for bone development mineralisation, as well as for eggshell formation, muscle and neural functions. Depending on the type of diets (veggie vs. non-veggie), species (turkey, chicken, duck), and age of animal (young vs. adult), limestone can contribute up to 95% total Ca in the final diet, with more Ca from limestone for younger animals with veggie diet. Despite the significant contribution of limestone to total diet Ca levels, the bioavailability variation of limestone Ca is rarely considered. Phosphorus (P) is a limited resource and is the third most costly ingredient in poultry …

Computational Investigation Of Calmodulin Photocontrol With The Help Of An Azobenzene Derivative, Jeremy Wells

Undergraduate Honors Theses

The ability to control the activity and binding capability of enzymes in a reversible manner offers tremendous control over biological processes. Photocontrol, in particular, is promising in that electromagnetic radiation can be fine-tuned in terms of its strength, location, and duration. Photosensitive compounds, such as the azobenzene family, experience an isomerization at certain wavelengths, and attaching these compounds to enzymes has the potential to alter their structure and activity. Calmodulin (CaM) is a Ca2+ -sensitive signaling protein that has the ability to affect several downstream processes in eukaryotes and is an excellent target for photocontrol due to its small size …

Severe Hypoxia Up-Regulates Gluconeogenesis In Daphnia, Morad C. Malek

Undergraduate Honors Theses

Hypoxia is a significant low oxygen state that has complex and diverse impacts on organisms. In aerobes, various adaptive responses to hypoxia are observed that vary depending on the level of oxygen depletion and previous adaptation, hence the continued attention to hypoxia as an important abiotic stressor. Adaptive responses to hypoxia are primarily governed by the hypoxia-inducible factors (HIFs), which activate downstream genetic pathways responsible for oxygen transport and metabolic plasticity. In aquatic habitats, oxygen availability can vary greatly over time and space. Therefore, aquatic organisms’ adaptation to hypoxia is likely pervasive, especially in genotypes originating from waterbodies prone to …

Free-Enterprise Farming On Grasslands In Central Nsw, Australia, Des Green, Stuart Green, Ted Wolfe, Jo Richardson, Rob Eccles

IGC Proceedings (1997-2023)

A pathway is described in developing and improving the pastures on family-owned sheep and beef properties at sites near Blayney in central NSW. Initially, the twin approach of sowing perennial grasses, predominantly phalaris (Phalaris aquatica) with subterranean clover (Trifolium subterraneum) plus the recommended addition of superphosphate fertiliser, was closely followed but within a decade ill-thrift in pastures and livestock occurred. Once the core problem of soil acidity was recognised, steps were taken to overcome this constraint with applications of lime. However, an additional modification involving the application of gypsum with lime had to be sorted out …

Calcium-Mediated Induction Of Vibrio Fischeri Es114 Biofilms, Katia Elizabeth Semenchuk

Master's Theses

Vibrio fischeri bacteria form biofilms that facilitate symbiotic colonization of the Hawaiian bobtail squid, Euprymna scolopes. These host-associated biofilms require production of the SYP polysaccharide. However, it has been difficult to observe robust biofilm formation in vitro in a laboratory setting using wild-type strain ES114; instead, most work investigating syp-dependent biofilm formation has relied on genetically altered strains. Guided by recent findings that increasing calcium induces c-di-GMP (Tischler et al., 2021), a molecule known in other systems to promote biofilm formation, the question was posed as to whether high levels of calcium alone could promote syp-dependent biofilm formation by ES114. …

The Impact Of Dietary Analyzed Calcium To Phosphorus Ratios And Standardized Total Tract Digestible Phosphorus To Net Energy Ratios On Growth Performance, Bone, And Carcass Characteristics Of Pigs, Hadley R. Williams, Mike D. Tokach, Jason C. Woodworth, Joel M. Derouchey, Robert D. Goodband, Jordan T. Gebhardt, Carine M. Vier, Ning Lu, Ron Navales, Matthew Spindler, Uislei Orlando, Luis Zaragoza, Alyssa M. Betlach

Kansas Agricultural Experiment Station Research Reports

A total of 2,184 pigs (PIC 337 × 1050; initially 27.3 lb) were used to evaluate the effects of feeding varying analyzed calcium to phosphorus (Ca:P) ratios at two levels of standardized total tract digestible (STTD) P:NE. Pens of pigs (26 pigs per pen) were assigned to 6 dietary treatments in a randomized complete block design with 14 pens per treatment. Diets consisted of two levels of STTD P:NE, including the PIC recommendation (1.8, 1.62, 1.43, 1.25, 1.10, and 0.99 g STTD P/Mcal NE from 25 to 50, 50 to 90, 90 to 130, 130 to 180, 180 to 230, …

Effects Of Vitamin D Administration At The Beginning Of Lactation In Dairy Cows Oninflammatory Response And Liver Metabolism, Feyyaz Kaya, Hasan Batmaz

Turkish Journal of Veterinary & Animal Sciences

This study aimed to investigate the effect of vitamin D administered 24 h after calving of dairy cows on calcium-phosphorus metabolism, carbohydrate, lipid and liver metabolism, and inflammatory response. Twenty multiparous Holstein cows were randomly assigned to the experimental (EG) and control group (CG). The EG received 5,000,000 IU vitamin D3 (5 mL) 24 h after parturition intramuscularly, whereas the CG group received 5 mL of 0.9% NaCl. Blood samples were taken 24 h after parturition before treatments and at the 3rd, 7th, 14th, and 28th days of lactation. Serum NEFA, BHBA, haptoglobin, serum amyloid A (SAA), ceruloplasmin, calcium, phosphorus, …

Primary Cilia Display A Non-Uniform Response To Intracellular Calcium Release, Hannah R. Fisher

WWU Graduate School Collection

Cilia are microtubule-based organelles that project from the surface of individual cells. When cilia are formed, they are nucleated from centrioles that are known as basal bodies. Microtubules elongating from the basal body give rise to the axoneme of the cilium, which gives cilia their rod-like structure. The axoneme is surrounded by a specialized plasma membrane that is unique, but continuous with the plasma membrane that surrounds the rest of the cell. The ciliary membrane is enriched with ion channels and membrane-bound proteins that are essential for cilia function. Interestingly, the cilium maintains a distinct environment from the rest of …

Development Of Fluorescence Based Approaches To Understand Astrocyte Biology In The Context Of Nicotine And Nicotinic Receptor Activity, Surya P. Aryal

Theses and Dissertations--Chemistry

Smoking and tobacco use (STU) is a major global health problem and worldwide more than six million people die due to tobacco related diseases each year. Although majority of smokers try to quit smoking several times in their life, traditional therapeutic approaches, which focus only on neuronal cells, have a very low success rate. Understanding the effect of nicotine on glial cells, synaptic communication and blood vasculature in the brain can provide further insights on the neurobiology of substance abuse and can potentially help to design better therapeutic approaches. Glial cells are non-excitable cells in the brain which do not …

Effects Of Dietary Calcium, Phosphorus And Microbial Phytase On Intestinal Morphologyin Laying Hens, Sabi̇re Güler, Ender Deni̇z Asmaz, Nuray Varol Kayapunar, İhsan İşbi̇li̇r, Şeri̇fe Şule Cengi̇z, Derya Yeşi̇lbağ, Ahmet Batuhan Şanli, Eyüp Eren Gültepe

Turkish Journal of Veterinary & Animal Sciences

Different challenges are being applied in the poultry industry in order to protect animal health and to increase immunity and production. The supplementation of microbial phytase is essential in terms of both reducing the inorganic phytase rate and contributing to the absorption of other minerals. In this study, a newly isolated microbial phytase was added at different concentrations to the diet together with calcium (Ca$^{2+}$) and available phosphorus (AP), and the effects of this supplementation on intestinal absorption capacity and Ca$^{2+}$ binding capacity were investigated via morphological measurements and immunohistochemical examination of the duodenum and ileum. For this purpose, 90 …

Agronomic Evaluation Of Forage Grasses Under Mature Rubber Plantation, N. L. Costa, Valdinei T. Paulino, A. C. M. Igreja, C. R. Townsend, J. A. Magalhães, R. G. De A Pereira, T. S. Paulino

IGC Proceedings (1997-2023)

The agronomic performance of seven forage grasses, under a mature rubber plantation, was assessed in a cutting trial carried out at Porto Velho, Rondônia. During the rainy season, the higher dry matter yields were obtained with B. brizantha, P. atratum BRA-9610 and B. humidicola. During the dry season, the grasses more productive were B. brizantha and P. atratum BRA-9610. In both seasons, magnesium and potassium contents did not vary among grasses. During the rainy season, the higher nitrogen and phosphorus contents were obtained with P. regnelli BRA-0159 and, P. guenoarum BRA-3824 and B. humidicola, respectively, while P. atratum …

Evaluation Of Centrosema Rotundifolium For Sand-Soil Savannas In Eastern Venezuela, I. Rodríguez, R. Schultze-Kraft, S. González

IGC Proceedings (1997-2023)

An evaluation of a collection of amphicarpic Centrosema rotundifolium showed considerable variability among the six accessions tested, regarding above- and below-ground seed production. Dry-season forage yields were very low but rainy-season yields reached acceptable levels. Contents of crude protein, P and Ca were intermediate to high (CP and P) or low (Ca). Three accessions are suggested for on-farm testing in association with grasses in order to explore the plant persistence implications of belowground seed production under practical conditions. Furthermore, the potential of this amphicarpic species for the conservation of sandy soils deserves attention.

The Effect Of Calcium Ions On Mechanosensation And Neuronal Activity In Proprioceptive Neurons, Devan E. Atkins, Kimberly L. Bosh, Grace W. Breakfield, Sydney E. Daniels, Makayla J. Devore, Hailey E. Fite, Landys Z. Guo, Danielle K. J. Henry, Alana K. Kaffenberger, Katherine S. Manning, Tatum E. Mowery, Cecilia L. Pankau, Nyla Parker, Malina E. Serrano, Yamaan Shakhashiro, Hannah N. Tanner, Ruth. A. Ward, Aubrey H. Wehry, Robin L. Cooper

Biology Faculty Publications

Proprioception of all animals is important in being able to have coordinated locomotion. Stretch activated ion channels (SACs) transduce the mechanical force into electrical signals in the proprioceptive sensory endings. The types of SACs vary among sensory neurons in animals as defined by pharmacological, physiological and molecular identification. The chordotonal organs within insects and crustaceans offer a unique ability to investigate proprioceptive function. The effects of the extracellular environment on neuronal activity, as well as the function of associated SACs are easily accessible and viable in minimal saline for ease in experimentation. The effect of extracellular [Ca²⁺] on …

Sources And Rates Of Phosphorus In Improving Degraded Pasture In Brazilian West Amazon, Valdinei T. Paulino, N. L. Costa, C. R. Townsend, J. A. Magalhães, R. G. A. Periera

IGC Proceedings (1997-2023)

The effects of phosphorus sources (triple superphosphate – TSP, single superphosphate – SSP and partially acidulated rock phosphate – PARP) and rates (0, 50 and 100 kg/ha of P₂O₅ on dry matter (DM) yields and chemical composition of a degraded Brachiaria brizantha cv. Marandu pasture were evaluated in a cutting trial. Phosphorus fertilization, irrespective of sources and rates, showed an effective agronomic technique for pasture recuperation. The grass DM yields and potassium contents were significantly improved by phosphorus application, while weed DM yields were depressed. Irrespective of sources and rates, crude protein, phosphorus, and calcium contents were …

Application Of Near Infrared Reflectance Spectroscopy (Nirs) For Macronutrients Analysis In Alfalfa (Medicago Sativa L.), A. Morón, D. Cozzolino

IGC Proceedings (1997-2023)

Near infrared reflectance spectroscopy was used to assess the mineral composition of alfalfa (Medicago sativa L.) as a tool for nutritional diagnosis. One hundred and ninety four (n = 194) samples of alfalfa from different locations representing a wide range of soils were used. Samples were reflectance scanned in a NIRS 6500 (NIRSystems, USA) instrument. The coefficients of determination (R²) of the regression estimate of the concentration of nitrogen, calcium, phosphorus, potassium, magnesium and sulphur and the errors in cross validation (SECV) were 0.93 (SECV: 1.6), 0.95 (SECV: 1.3), 0.93 (SECV: 1.9), 0.88 (SECV: 2.8), 0.82 (SECV: …

Nutrient Dynamics And Inventory In Tropical Grassland Ecosystem In Southern India, K. Karunaichamy, Kailash Paliwal

IGC Proceedings (1997-2023)

The present study was to ascertain the distribution of calcium (Ca), magnesium (Mg) and sodium (Na) in the different plant components and in the soil top 30 cm at Kalikesam in Western Ghats region of southern India. Magnesium concentration increased in the order: dead shoots > belowground > litter > aboveground live. Sodium concentration in live shoots was maximum in February (0.51%) and minimum in August (0.05%). Annually 8.94 g/m² Ca, 2.41g/ m² Mg and 2.30g/ m² Na was taken up by plants. The distribution of the three nutrients in plant/soil system indicated that the major portion of the nutrients …

Mineral Nutrition In Broilers: Where Are We At?, Rick J. Kleyn, Mariana Ciacciariello

Proceedings of the Arkansas Nutrition Conference

Mineral nutrition remains an essential consideration for broiler nutritionists. The complex interactions between different minerals sources, other ingredients, and the broiler itself, coupled with their relatively low costs, have led to the current status of elevated levels of minerals in broiler diets. New perspectives on sustainability have brought about a rethink of the way we formulate practical diets. There are considerable opportunities for reducing macro minerals such as calcium and phosphorus, particularly for older birds. Our understanding of attaining an ideal dietary cation-anion balance is confused by the lack of clarity in measuring the balance and the bird’s ability to …

The Influence Of Incubation Conditions On Oxygen Consumption During The Development Of Pantherophis Guttatus, Celeste Gallardo

Electronic Theses and Dissertations

The flexible shell of some oviparous reptiles has led to differences in nutrient mobilization and water relations when compared to their rigid-shelled counterparts. Flexible-shelled eggs gain more water during development, and because of the shell structure, yolk calcium content is higher than that of eggshells. When water availability is altered for reptiles with flexible-shelled eggs, differences in both energy and nutrient utilization are observed. This study was designed to determine the baseline metabolic rate during development for the snake, Pantherophis guttatus under normal conditions, and to observe how changes in eggshell calcium and water availability impact embryonic oxygen consumption. Eggshell …

Net Geochemical Release Of Base Cations From 25 Forested Watersheds In The Catskill Region Of New York, Sara C. Nieman, Chris E. Johnson

Civil and Environmental Engineering

Chemical weathering of minerals is the principal mechanism by which base cations (Ca²⁺, Mg²⁺, K⁺, and Na⁺) are released and acidity is neutralized in soils, bedrock and drainage waters. Quantifying the release of base cations from watershed soils is therefore crucial for the calculation of “critical loads” of atmospheric acidity to forest ecosystems. We used a mass-balance approach to estimate the rate of release of base cations in 25 headwater catchments in the Catskill region of New York, an area historically subject to high inputs of acid deposition. In 2010-2013, total net …

Distribution Of Calcium And Its Reserve Forms In Widespread Irrigated Gray Meadow Soils Of The Syrdarya Region, A. Akhatov

Irrigation and Melioration

The article examines the uneven distribution of calcium in reserve (general, potential, close and labile) forms in irrigated and gray-earth-meadow soils of the experimental section of the NIISSAVKH of the Gulistan district of the Syrdarya region and the farms "Sokhil" and "Ak altin" in irrigated meadow soils the form of a close reserve in the soil profile is 310 mg per 100 gr of soil. The upper layer of highly saline, medium loamy soil gradually decreases at a depth of 60-76 cm. layer. The labile calcium reserve in irrigated serozem-meadow, highly saline, medium loamy soils varies from 175 to 260 …