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The Effect Of Nitrate Supplementation On Exercise Tolerance And Performance: A Systematic Review And Meta-Analysis, Gavin Van De Walle Jan 2017

The Effect Of Nitrate Supplementation On Exercise Tolerance And Performance: A Systematic Review And Meta-Analysis, Gavin Van De Walle

Electronic Theses and Dissertations

The purpose of this paper was to systematically review the current literature and evaluate the overall efficacy of nitrate supplementation on exercise tolerance and performance by meta-analysis. Studies were eligible for inclusion if they met the following criteria: 1) were an experimental trial published in an English peer-reviewed journal; 2) compared the effects of inorganic nitrate consumption with a non-bioactive supplement control or placebo; 3) used a quantifiable measure of exercise performance; and 4) was carried out in apparently healthy participants without disease. A total of 29 studies were identified that investigated the effects of nitrate supplementation on exercise tolerance …


An Evaluation Of Deer And Pronghorn Surveys In South Dakota, Kristopher W. Cudmore Jan 2017

An Evaluation Of Deer And Pronghorn Surveys In South Dakota, Kristopher W. Cudmore

Electronic Theses and Dissertations

To properly manage white-tailed deer (Odocoileus virginianus), mule deer (Odocoileus hemionus), and the American pronghorn antelope (Antilocapridae americana), wildlife managers must identify population perimeters, sample size, age and sex ratios of these three species. The primary objective of this study was to evaluate age and sex ratios and determine a minimum sample size for each study area for all deer species and pronghorn, in addition to deriving a methodology and population estimate for white-tailed deer in the Black Hills. Sample size needed for both species of deer and pronghorn ranged from 60-70 groups of does. Age ratios calculated from daylight …


Effects Of Phase Feeding During Gestation On Gilt Performance, Offspring Quality And Robustness, Agatha Ampaire Jan 2017

Effects Of Phase Feeding During Gestation On Gilt Performance, Offspring Quality And Robustness, Agatha Ampaire

Electronic Theses and Dissertations

A total of 51 gilts in 6 blocks were randomly assigned to one of 3 feeding regimens: Constant (Constant-f), 2.21 kg/d of a standard diet from breeding to d 112 of gestation (1.7 g Lys/kcal ME; 3276 kcal ME/kg); Bump feeding (Bump-f), the standard diet at 2.21 kg/d from breeding to d 89 and 2.61 kg/d from d 90 - 112; Phase feeding (Phase-f ), 2.21 kg/d from breeding to d 89 (1.5 g Lys/kcal ME; 3275 kcal ME/kg) and 2.61 kg/d from d 90 - 112 (2.1 g Lys/Kcal ME; 3290 Kcal ME/kg) over 2 reproductive cycles. Sows received …


Effect Of Grazing Pressure On Cattle Grazing Cool Season Annual Forages, Brooke Brunsvig Jan 2017

Effect Of Grazing Pressure On Cattle Grazing Cool Season Annual Forages, Brooke Brunsvig

Electronic Theses and Dissertations

Ruminants can use plant fiber to produce food products that provide nourishment to humans. However, a precise understanding of specific plants selected by grazing ruminants remains elusive. Many long- and short-term factors impact cattle grazing behavior. Ultimately, grazing behavior can affect forage available for grazing, nutrient density of forage, dry matter intake, and animal performance. A myriad of grazing management strategies have been developed utilizing animal behavior to allow more efficient use of forage resources. Many management strategies based on manipulation of grazing behavior are simple and cost effective. Optimal performance of individual animals and amount of animal products produced …


Diaporthe, Soybean Cyst Nematode, And Soybean Aphid: An Evaluation Of Potential Interactions Occurring Among Pests On Soybean In South Dakota, John Philip Posch Jan 2017

Diaporthe, Soybean Cyst Nematode, And Soybean Aphid: An Evaluation Of Potential Interactions Occurring Among Pests On Soybean In South Dakota, John Philip Posch

Electronic Theses and Dissertations

Soybean Glycine max (L.) Merr., is an important crop grown in South Dakota, with an estimated production of $2.33 billion in 2015. However, soybean production in South Dakota is compromised by four pests, the fungal pathogens Diaporthe longicolla (Hobbs) Santos, Vrandecic, and Phillips and Diaporthe caulivora (Athow and Caldwell) Santos, Vrandecic, and Phillips, the soybean cyst nematode Heterodera glycines Ichinohe (SCN), and the soybean aphid, Aphis glycines Matsumura (Hemiptera: Aphididae). Additionally, these pest can co-occur in fields within South Dakota and the implications associated with interactions among these pests are unknown. We hypothesized that both Diaporthe species would interact with …


Identification Of Physiological And Morphological Traits Governing High Water Use Efficiency In Alfalfa, Krishna Ghimire Jan 2017

Identification Of Physiological And Morphological Traits Governing High Water Use Efficiency In Alfalfa, Krishna Ghimire

Electronic Theses and Dissertations

Alfalfa is an important forage crop worldwide. Being deep-rooted, N2-fixing and high yielding, alfalfa has great economic, ecological and nutritional benefits. While alfalfa is a high yielding crop, its high productivity depends on irrigation water in many areas and consumes the greatest amount of water among all the major crops. With a growing demand for water resources due to an increase in human population and industrial water use, plus frequent drought due to climate change, irrigation water has become increasingly scarce and expensive. To sustain high production of alfalfa with limited water resource, alfalfa cultivars with improved water use efficiency …


An Integrated Evaluation Of The Conservation Reserve Enhancement Program In South Dakota, Jarrett D. Pfrimmer Jan 2017

An Integrated Evaluation Of The Conservation Reserve Enhancement Program In South Dakota, Jarrett D. Pfrimmer

Electronic Theses and Dissertations

Grassland restoration efforts in North America typically share the goal of improving ecological conditions for wildlife; however, it is unclear in many cases if goals are met. The South Dakota Conservation Reserve Enhancement Program (CREP) was initiated to alleviate agriculturally-related environmental degradation by converting 40,469 hectares of eligible cropland and marginal pastureland to perennial vegetation. The program aims to provide habitat for obligate grassland breeding songbirds, while producing an additional 285,000 pheasants and 60,000 ducks annually. As part of a collaborative comprehensive evaluation effort, my research assessed the response of grassland-dependent breeding birds to CREP implementation at varying spatial scales …


Interactive Effects Of Cover Crops, Invertebrate Communities And Soil Health In Corn Production Systems, Claire Lacanne Jan 2017

Interactive Effects Of Cover Crops, Invertebrate Communities And Soil Health In Corn Production Systems, Claire Lacanne

Electronic Theses and Dissertations

The adoption of regenerative farming practices is gaining traction, but the costs and benefits are not often considered on a systems level. Encouraging biodiversity and soil health is the goal of many agricultural practices used in regenerative farming; regenerative systems employ practices which abide by the two main principles of increasing biodiversity and decreasing disturbance, with the goal of encouraging ecosystem functioning to minimize inputs and maximize the productivity of a farm. I examined the management of corn (Zea mays) fields across four states in the Upper Midwest region of the United States. Regenerative systems in this study …


Heterogeneous Nitrogen Losses: Cost-Effectiveness Analysis Of Changes In Management Across South Dakota, Archibold Quaye Jan 2017

Heterogeneous Nitrogen Losses: Cost-Effectiveness Analysis Of Changes In Management Across South Dakota, Archibold Quaye

Electronic Theses and Dissertations

The loss of nitrogen fertilizer into the atmosphere and waterways is of increasing concern for citizens and policy makers. This is particularly relevant for hypoxia in rivers, lakes, and oceans, but also relevant for policy makers in reducing the increasing concentration of greenhouse gases (GHG) in the atmosphere. GHGs trap heat in the atmosphere and include: carbon dioxide, methane, nitrous oxide and fluorinated gases. Overall, the estimated contribution from the agricultural sector to GHG emissions was 9% in 2013 (EPA, 2013). Further, the addition of nitrogen to the soil through the use of synthetic fertilizers is a main contributor to …


Phenotypic Plasticity Of Native And Invasive Cool-Season Grasses In Response To Frequency Of Moisture Availability, Ming-Yu Stephens Jan 2017

Phenotypic Plasticity Of Native And Invasive Cool-Season Grasses In Response To Frequency Of Moisture Availability, Ming-Yu Stephens

Electronic Theses and Dissertations

Phenotypic plasticity, the ability of an individual to alter its growth in response to environmental conditions, is an attribute that is considered a likely invader attribute, as it provides the opportunity to expand its ecological niche breadth. Adaptive phenotypic plasticity can affect not only the ability to establish in a new environment, but also the ability to outperform the existing vegetation over time. Bromus inermis (smooth brome) and Poa pratensis (Kentucky bluegrass) are invasive species in tall- and mixed-grass prairie ecosystems. The objectives of this two-part study were to quantify the adaptive plasticity of biomass accumulation and tiller production in …


Cbl And Cbl-B Dictate Csf-1r Endocytic Traffic And Signaling In Macrophages, Lu Huang Jan 2017

Cbl And Cbl-B Dictate Csf-1r Endocytic Traffic And Signaling In Macrophages, Lu Huang

Electronic Theses and Dissertations

Macrophage colony stimulating factor receptor (CSF-1R or MCSFR) is a receptor tyrosine kinase essential for the growth and function of macrophages. Understanding the mechanisms that regulate CSF-1R activation and deactivation will provide insights to clinical treatment of macrophage related diseases including chronic inflammation and cancer. Previously, our laboratory showed that CSF-1R undergoes a novel membrane trafficking route that involves macropinocytosis to deactivate CSF-1R signaling. This thesis makes the discovery that the ubiquitin ligases Cbl and Cbl-b cooperate to regulate CSF-1R endocytosis and traffic to macropinosome in macrophages. Macrophages were derived from mice knocked out for Cbl, Cbl-b or the double …


Response Of Soil Properties To 23-25 Years Of Diverse Crop Rotations And Tillage Systems In South Dakota, Usa, Abdullah H. Alhameid Jan 2017

Response Of Soil Properties To 23-25 Years Of Diverse Crop Rotations And Tillage Systems In South Dakota, Usa, Abdullah H. Alhameid

Electronic Theses and Dissertations

Diverse cropping systems and no-till (NT) are beneficial in improving soil organic carbon (SOC) and other soils properties. The present study was conducted to evaluate the interactions and impacts of long-term tillage and diverse crop rotation systems on soil quality indicators that include a range of soil chemical, physical, hydrological, and microbial properties. The study was established in 1991 at Beresford, South Dakota and included three crop rotation systems [2-yr; maize (Zea mays L.)-soybean (Glycine max L.), 3-yr; maize-soybean-oat (Avena sativa L.), and 4-yr; maize-soybean-oat-wheat (Triticum aestivum L.)] managed under two tillage systems (NT and CT; conventional- tillage). Soil sampling …


The Influence Of Chromium Supplementation On The Insulin Pathway, Carcass Traits, And Meat Quality Of Feedlot Steers, Heather Rode Jan 2017

The Influence Of Chromium Supplementation On The Insulin Pathway, Carcass Traits, And Meat Quality Of Feedlot Steers, Heather Rode

Electronic Theses and Dissertations

Chromium supplementation (Cr) can increase efficiency of growth in livestock by amplifying insulin signaling. Previous research showed Cr increases nutrient utilization and changes in muscle growth and fat deposition. Therefore, the objective of this research was to determine how Cr affects carcass traits, meat quality, and the insulin pathway in feedlot steers. Calf-fed steers were allotted to 0 ppb (CON) or 400 ppb Cr propionate (Cr400) for 210d in experiment 1. Two longissimus muscle (LM) biopsies were collected to examine protein abundance and gene expression after adaptation to a terminal implant and after adaption to ractopamine hydrochloride (RAC). A follow …


An Evaluation Of The Relationship Between Cooking And Wellness Behaviors In College Students, Andrea Hanson Jan 2017

An Evaluation Of The Relationship Between Cooking And Wellness Behaviors In College Students, Andrea Hanson

Electronic Theses and Dissertations

Background: Few studies have examined the relationship between cooking and wellness behaviors in college students.
Objective: Determine the association between cooking, wellness behaviors, fruit and vegetable (FV) intake and body mass index (BMI) in college students.
Design and Participants: A cross-sectional analysis was conducted using baseline data a multi-state, Community-Based Participatory Research project aimed at preventing weight gain in first-year college students. Physical activity (PA), cooking (type, frequency and confidence), intention for meal planning (MP), mealtime behaviors, and cups of FV, were measured using validated survey tools. BMI was calculated from measured height and weight. Study participants were first-year students …


A Comparison Of Land Use And Land Cover In Public Lands Of The Northwestern Great Plains And High Plains Ecoregions And The Implications For Grassland Birds, Dawn L. Siemonsma Jan 2017

A Comparison Of Land Use And Land Cover In Public Lands Of The Northwestern Great Plains And High Plains Ecoregions And The Implications For Grassland Birds, Dawn L. Siemonsma

Electronic Theses and Dissertations

Loss and degradation of grassland habitat are driving forces that contribute to widespread declines of grassland birds in the United States. Many studies have evaluated habitat needs for the conservation of grassland birds, but the relative contribution of public lands in representing and maintaining avian biodiversity remains poorly understood. Having a better understanding of the role that publicly managed grasslands play in the conservation of grassland bird habitat is important for assessing the value of the investment the American public makes in these lands. Therefore, I investigated spatial relations among variations in amounts and distributions of publicly owned and managed …


Using Rna Seq To Define Genes And Pathways Affecting Salmonella Vaccine Response In Beef Cattle, Sara Awrahman Jan 2017

Using Rna Seq To Define Genes And Pathways Affecting Salmonella Vaccine Response In Beef Cattle, Sara Awrahman

Electronic Theses and Dissertations

A Salmonella siderophore receptor and porin (SRP) proteins vaccine is an intervention strategy to control Salmonella burden in cattle. The vaccine works by stimulating immunity to produce antibodies against bacterial SRPs as siderophore molecules play a major role in transporting iron to bacteria. Blocking iron uptake system by antibodies causes death of bacterial cells. The vaccine can also be useful to protect humans against salmonellosis, which causes high rates of illness and death annually, by reducing shedding of Salmonella in the feces of cattle. Though other researchers have evaluated efficacy and immune response of this Salmonella SRP vaccine, genes …


Evaluation Of A Bioprocessed Soybean Meal On Nursery Pig Immune Status And Disease Resistance, Jacob R. Koepke Jan 2017

Evaluation Of A Bioprocessed Soybean Meal On Nursery Pig Immune Status And Disease Resistance, Jacob R. Koepke

Electronic Theses and Dissertations

Weaning is a stressful time in a piglet’s life that can have negative impacts on performance and health if not managed properly. The objective of this research was to evaluate the effects of a bioprocessed SBM alone or in combination with spray-dried plasma (SDP) or fishmeal (FM) on immune response at weaning with no imminent disease threat and during a porcine reproductive and respiratory virus (PRRSV) challenge. In the first trial, 239 pigs (initial BW 6.56 ± 0.87 kg, 21 d of age) were used in a 35-d study and allotted to one of 4 dietary treatments (10 pens/trt) according …


Using Gluten Index To Improve Spring Wheat Loaf Volume Prediction, Yaming Lu Jan 2017

Using Gluten Index To Improve Spring Wheat Loaf Volume Prediction, Yaming Lu

Electronic Theses and Dissertations

Wheat (Triticum aestivum L.) is the most widely cultivated crop in the world and contributes about 20% of the total dietary calories and proteins globally. Unique properties of doughs formed from wheat flour make it feasible to produce a range of food, including bread. Loaf volume is the most perceptible indicator for breadmaking quality. Selection in the early generations for loaf volume is difficult due to the requirement of the large volume of grains and due to the costly, time-consuming, and labor-intensive evaluation process. Identification of simple and reliable predictive tests for loaf volume is highly desirable. This study …


Effects Of Specific Home Environment Factors On A Child's Weight, Molly Millage Jan 2017

Effects Of Specific Home Environment Factors On A Child's Weight, Molly Millage

Electronic Theses and Dissertations

Background: In 2011-2014 8.9% of children 2-5 years old were classified as obese. Obesity in children predisposes them to negative health and psychological consequences. One of the most significant factors that influences a child is the physical environment the child is exposed to, which includes the home environment.
Objective: The purpose of this study was to examine how the home environment ie. cooking equipment, food preparation/availability, and frequency of family meals eaten at home, contribute to childhood obesity in children 3-5 years old.
Design: This study was a cross-sectional analysis of the iGrow Readers study.
Participants/setting: Total of 229 parent/child …


Genetic Interference And Receptor Biology Of Neglected Influenza Viruses, Runxia Liu Jan 2017

Genetic Interference And Receptor Biology Of Neglected Influenza Viruses, Runxia Liu

Electronic Theses and Dissertations

Influenza B virus (IBV) is an important pathogen that infects humans and causes seasonal influenza epidemics. By using next-generation sequencing (NGS) approach, we analyzed total mRNAs extracted from A549 cells infected with B/Brisbane/60/2008, and identified four defective genomes in IBV with two from the polymerase basic subunit 1 (PB1) segment and the other two from the matrix (M) segment. Each of them can potently inhibit the replication of IBV. One derived from PB1 segment was able to interfere modestly with influenza A virus (IAV) replication. The productions of the four defective RNAs are not dependent on the cell types. The …


Development Of Best Management Practices For Production Of Ethiopian Mustard (Brassica Carinata) In South Dakota, Phillip Kenneth Alberti Jan 2017

Development Of Best Management Practices For Production Of Ethiopian Mustard (Brassica Carinata) In South Dakota, Phillip Kenneth Alberti

Electronic Theses and Dissertations

Ethiopian mustard (Brassica carinata) is an alternative non-food oilseed crop which has received interest for its potential as a low-input option for production in the Northern Great Plains (NGP). As B. carinata is a new crop to the NGP, the best management practices have yet to be developed. The overall goal of this project was to develop best management practices for B. carinata production in two diverse agroenvironments in South Dakota. Two field experiments were conducted to assess the response of two B. carinata varieties to i) four seeding rates (4.5, 9, 13.5, and 18 kg ha-1 …


Native Species For Biomass Production And Roadside Habitat In South Dakota, Jacob Foley Jan 2017

Native Species For Biomass Production And Roadside Habitat In South Dakota, Jacob Foley

Electronic Theses and Dissertations

Native grasses and forbs are being considered for planting along South Dakota roadsides to create a favorable habitat for wildlife while also allowing landowners a source of forage and biomass production. South Dakota is host to 54,900 hectares (145,000 acres) of right-of-way that are managed by the South Dakota Department of Transportation. Approximately 80% of the roadsides are used by landowners for hay production. Species diversity and potential biomass yield were determined for the current roadside vegetation along four transects (SD Highways 14, 34 and 50) and SD I- 29 between White, SD and Elk Point, SD. Smooth bromegrass (Bromus …


Invertebrate Prey Selectivity Of Channel Catfish (Ictalurus Punctatus) In Western South Dakota Prairie Streams, Erin D. Peterson Jan 2017

Invertebrate Prey Selectivity Of Channel Catfish (Ictalurus Punctatus) In Western South Dakota Prairie Streams, Erin D. Peterson

Electronic Theses and Dissertations

Benthic invertebrates are an important resource for aquatic consumers and should be considered in management of sportfish populations as a factor influencing fishery health. Our study seeks to draw a relationship between invertebrate prey availability and the Channel Catfish Ictalurus punctatus fishery of western South Dakota through diet analysis, but invertebrate surveys in the area have not focused on invertebrates as a prey source. Our objectives were to characterize patterns in availability of the invertebrate prey base in five major river basins, and to evaluate the effects of availability on Channel Catfish prey selectivity and condition. Invertebrates were collected at …


Genetic Factors Associated With Thermal Tolerance In Grow-Finish Pigs As Measured By Feeding Behavior, Amanda Jeanne Cross Jan 2017

Genetic Factors Associated With Thermal Tolerance In Grow-Finish Pigs As Measured By Feeding Behavior, Amanda Jeanne Cross

Electronic Theses and Dissertations

The objectives of this study were: one, use electronic monitoring to determine feeding behavior patterns of grow-finish pigs throughout the year and to identify changes that occurred during heat stress events, and second, identify genetic markers associated with changes in feeding behavior due to heat stress. Pigs were placed in a grow-finish barn at approximately eight to ten weeks of age in 6 pens of 40 animals and monitored for 4-months. Gilts and barrows were from three different sire breeds, Duroc, Landrace, and Yorkshire. Each pen had one feeder, designed to feed 5 animals at a time. Feeders were fitted …


Improving Survey Methodology To Monitor Rare Grassland Birds In South Dakota, Kassondra Hendricks Jan 2017

Improving Survey Methodology To Monitor Rare Grassland Birds In South Dakota, Kassondra Hendricks

Electronic Theses and Dissertations

Breeding Bird Survey (BBS) data for grassland bird species has shown the most rapid population decline of any other bird group. Current roadside survey techniques, however, may fall short of providing accurate numbers of rare grassland bird species such as chestnut-collared longspur (Calcarius ornatus), lark bunting (Calamospiza melanocorys), Sprague’s pipit (Anthus spragueii), and Baird’s sparrow (Ammodramus bairdii). Trends resulting from roadside data for grassland birds are oftentimes determined to be statistically insignificant because many grassland bird species occur on too few routes, occur in low numbers per route, and show high annual fluctuations in number. It is possible roadside surveys …


Local And Systemic Actions Of Gnrh Relating To Estradiol And Associated Reproductive Parameters In Beef Cattle, Jerica J.J. Rich Jan 2017

Local And Systemic Actions Of Gnrh Relating To Estradiol And Associated Reproductive Parameters In Beef Cattle, Jerica J.J. Rich

Electronic Theses and Dissertations

In cattle, estradiol is responsible for modulating many mechanisms involved in successful reproduction. Specifically estradiol is the primary signal for the initiation of standing estrus (Allrich, 1994), and cattle expressing estrus prior to fixed time AI (FTAI) have been reported to have increased preovulatory estradiol concentrations (Perry and Perry, 2008) and increased pregnancy success compared to animals that did not exhibit estrus (Richardson et al., 2016). Gonadotropin releasing hormone, classically, stimulates estradiol production via the two-cell two-gonadotropin hypothesis (Fortune and Quirk, 1988), and GnRH administered systemically in small doses (5 μg) has been reported to elicit an LH pulse similar …


Evaluation Of The Deadwood Bighorn Sheep Herd Translocation, Ty J. Werdel Jan 2017

Evaluation Of The Deadwood Bighorn Sheep Herd Translocation, Ty J. Werdel

Electronic Theses and Dissertations

From 2015-2017, we evaluated a newly established bighorn sheep (Ovis canadensis) herd in the Deadwood Region of the Black Hills, South Dakota. Our objectives were to 1a) determine annual survival rates, 1b) determine cause-specific mortality, 1c) estimate population size, 2a) assess genetic diversity, 2b) assess disease prevalence, 3) evaluate movement patterns post-release, 4a) evaluate 3rd-order habitat selection, and 4b) estimate herbaceous biomass at foraging sites post-release of translocated bighorn sheep. In February 2015, we captured and translocated 26 bighorn sheep from the Luscar Mine near Hinton, Alberta, Canada to the Deadwood Region of the Black Hills, South Dakota. Overall annual …


Using Remote Sensing To Estimate Crop Water Use To Improve Irrigation Water Management, Arturo Reyes-Gonzalez Jan 2017

Using Remote Sensing To Estimate Crop Water Use To Improve Irrigation Water Management, Arturo Reyes-Gonzalez

Electronic Theses and Dissertations

Irrigation water is scarce. Hence, accurate estimation of crop water use is necessary for proper irrigation managements and water conservation. Satellite-based remote sensing is a tool that can estimate crop water use efficiently. Several models have been developed to estimate crop water requirement or actual evapotranspiration (ETa) using remote sensing. One of them is the Mapping EvapoTranspiration at High Resolution using Internalized Calibration (METRIC) model. This model has been compared with other methods for ET estimations including weighing lysimeters, pan evaporation, Bowen Ratio Energy Balance System (BREBS), Eddy Covariance (EC), and sap flow. However, comparison of METRIC model outputs to …


An Evaluation Of Nitrogen Supplementation And Processed Soy Fractions On The Performance Of Cultured Fishes, Brandon M. White Jan 2017

An Evaluation Of Nitrogen Supplementation And Processed Soy Fractions On The Performance Of Cultured Fishes, Brandon M. White

Electronic Theses and Dissertations

World population growth has resulted in an increased demand for a sustainable food supply. This rapid growth coupled with an increased per capita consumption of seafood, has resulted in many of the world’s marine fisheries being over-exploited in an effort to meet the global demand for seafood. Aquaculture has attempted to fill the supply and demand gap created by the dwindling supply of fish in the world’s oceans. Fishmeal (FM) has traditionally been the primary protein ingredient in aquafeeds fed to farm-raised fish, however its unstable supply and increasing price have driven researchers to identify alternative protein sources. Soybean meal …


Macropinosome Maturation Is A Clathrin Dependent Process In Bone Marrow Macrophages, Susmita Poudel Jan 2017

Macropinosome Maturation Is A Clathrin Dependent Process In Bone Marrow Macrophages, Susmita Poudel

Electronic Theses and Dissertations

Macrophages nonspecifically take up extracellular fluids, solutes and macromolecules by macropinocytosis. Understanding the mechanisms of macropinosome maturation will inform the study of lipid uptake, viral entry, antigen processing and presentation, as well as regulation of cell growth. Colony stimulating factor-1 receptor (CSF-1R) is internalized by small vesicle endocytosis, trafficked to nascent macropinosomes and degraded. These CSF-1R positive macropinosomes mature through a sequence similar to endosomes, progressing from EEA1 and Rab5 to Rab7 positive vesicles before fusing with lysosomes. Here we report the assembly of clathrin on internalized macropinosomes shown both by live-cell microscopy of cells expressing clathrin light chain-yellow fluorescent …