Life Sciences Commons^™

Characterization Of Calcium Homeostasis Parameters In Trpv3 And Cav3.2 Double Null Mice, Aujan Mehregan

Masters Theses

In mammals, calcium influx is required for oocyte maturation and egg activation, as it supports the persistent calcium oscillations induced by fertilization. These oscillations are required for the initiation of embryo development. The molecular identities of the plasma membrane calcium-permeant channels that underlie calcium influx are not established. Among these channels, Transient Receptor Potential Vanilloid, member 3 (TRPV3) allows divalent cations, namely strontium (Sr²⁺) and calcium (Ca²⁺) with high permeability, into cells, and its expression pattern seems to predict an essential role in the initiation of development. Another channel that was identified to be expressed in …

Of Rats And Men, Thomas S. Walsh

Capstones

This capstone is a data-driven investigation into New York City's rat problem. By using publicly available government data to map rat activity in NYC, I identified several socio-economic variables that correlate with rat populations at the community district, borough, and city-scale. I used these findings (mainly that rat problems are linked to lower incomes) as the basis of an investigation, which includes interviews with residents, experts, and city officials. Prof. Bobby Corrigan, urban rodentologist and formerly with the NYC Department of Health criticizes the city's efforts for the first time on the record.

https://thomasseiyawalsh.wixsite.com/ratstone

Resource Selection, Survival, And Departure Of Adult Female Mallards From The Lake St. Clair Region During Autumn And Winter, Matthew Palumbo

Electronic Thesis and Dissertation Repository

During autumn and winter, mallards (Anas platyrhynchos) maximize fitness through their spatiotemporal distribution to avoid mortality risks while balancing trade-offs to access foods to undergo migration and maintain homeostasis. Thousands of mallards use Lake St. Clair as it is an important, but threatened, migratory staging area in the Great Lakes. My goal was to understand how mallards were selecting resources in the region and potential relationships of selection strategies. My objectives were to estimate resource selection of adult female mallards, in relation to perceived risk of hunting mortality, and determine if selection strategies were related to survival and …

Four Legs Good, Two Legs Bad: How Slaughterhouse Safety Hasn’T Kept Up With The Times, Emily Ziemski

Capstones

From legal loopholes and outdated rules to undetectable infections and understaffing, the United States Department of Agriculture may not be doing all it can to make sure the American public’s health isn't at risk. As the people of the United States consume over 500 million pounds of beef a year, food safety policy and slaughterhouses are falling behind in proper procedural measurements in the beef industry.

emziemski.com/capstone

Organizational Effects Of Defeminizing Toxicants: Lessons Learned From An Environmental Sentinel Organism, The Fathead Minnow., Jonathan Ali

Theses & Dissertations

Endocrine disrupting compounds (EDCs) are chemicals that interfere with hormone function and are increasingly detected in aquatic environments, where they elicit adverse effects from exposed organisms. The toxicological effects of EDCs can be described as either activational (reversible) or organizational (irreversible), where the latter are associated with adverse outcomes in reproductive performance of adult fish. However, few studies have investigated the organizational impacts of anti-estrogenic or “defeminizing” EDCs, e.g. agrichemicals or pharmacological agents, in an environmentally-relevant or “sentinel” species. The objective of this study was to investigate the impacts of early-life EDC-initiated changes in estrogenic gene expression on organizational effects …

Effects Of Crude Oil On Tumor Suppressor P53 Polymorphisms In Laboratory-Exposed Atlantic Killifish, Fundulus Heteroclitus, Allison Margaret Nadler

Seton Hall University Dissertations and Theses (ETDs)

Polycyclic aromatic hydrocarbons (PAHs), constituents of crude oil, are implicated as a potent source of adverse toxicological effects on living organisms. To model the effects of PAHs in response to environmental oil spill disasters a species of killifish (Fundulus heteroclitus) was captured and exposed to crude oil in a laboratory-controlled setting. Over a period of 7 days, fish were dosed with crude oil by gavage, culled, and organs were harvested for analysis. Excitation-emission matrix spectroscopy (EEMS) of gall bladder tissue homogenates was used to verify exposure. Effects of PAHs on the p53 gene were evaluated as an indicator …

Regulation Of Liver Mitochondrial Metabolism During Hibernation By Post-Translational Modification, Katherine E. Mathers

Electronic Thesis and Dissertation Repository

Hibernation, characterized by a seasonal reduction in metabolism and body temperature, allows animals to conserve energy when environmental conditions (e.g. temperature, food availability) are unfavourable. During hibernation, small mammals such as the 13-lined ground squirrel (Ictidomys tridecemlineatus) cycle between two distinct metabolic states: torpor, where metabolic rate is suppressed by >95% and body temperature falls to ~5 °C, and interbout euthermia (IBE), where metabolic rate and body temperature rapidly increase and are maintained at euthermic levels several hours. Suppression of metabolism during entrance into torpor is paralleled by rapid suppression of liver mitochondrial metabolism. In my thesis, I …

Habitat Use Of Blacktip Sharks (Carcharhinus Limbatus) At Fishing Piers, Kelsey L. Spencer

Electronic Theses and Dissertations

Blacktip sharks (Carcharhinus limbatus) can be observed near fishing piers throughout the summer along the northeast coast of South Carolina. These piers attract and support a wide variety of potential prey and sharks are able to forage on fishers’ discards with minimal energetic cost. I tagged 12 blacktip sharks with acoustic transmitters, monitored piers with acoustic receivers, and conducted pier-creel surveys to determine the habitat use of blacktip sharks at fishing piers, factors that influenced residence time and presence/absence at piers, and any cyclical patterns in visits to piers. Data were analyzed with pier association indices (PAI), mixed models, and …

Identification And Quantification Of Cyanide And Its Metabolites In Lemur Urine, Jeremy Peralta

Student Theses

Cyanide is a chemical compound that contains the monovalent groups of carbon and nitrogen. This substance is considered a rapidly acting deadly chemical. However, some animals such as lemurs consume food products that are known to contain elevated levels of cyanide without major consequences. The mechanism by which the lemur can handle this high exposure to cyanide is unknown. In this study, we developed and validated two analytical methods for the determination of cyanide and two metabolites, thiocyanate and 2-aminothiazoline-4-carboxylic acid (ATCA) in lemur urine. The method was applied to 47 authentic lemur urine samples collected from 4 different species …

Forecasting The Spread And Invasive Potential Of Apple Snails (Pomacea Spp.) In Florida, Stephanie A. Reilly

HCNSO Student Theses and Dissertations

Forecasting the potential range of invasive species is a critical component for risk assessment, monitoring, and management. However, many of these invasive species are not yet at equilibrium which can be problematic for many modelling approaches. Using the climate matching method, MaxEnt, a series of species distribution models (SDMs) and risk analysis maps were created for select apple snail species in Florida: Pomacea canaliculata, P. diffusa, and P. maculata. Apple snails, freshwater gastropods in the family Ampullariidae, are native to South America and were introduced to the United States via the pet trade approximately 40 years ago. …

Lake Sturgeon (Acipenser Fulvescens) Trophic Position And Movement Patterns In The Lower Niagara River, Ny, Eric Bruestle

Great Lakes Center Masters Theses

Lake sturgeon Acipenser fulvescens were once widely distributed throughout the Laurentian Great Lakes. However, widespread overharvest and habitat degradation has diminished their numbers. The lower Niagara River, NY contains one of the few remaining recovering populations of lake sturgeon in New York State. The goal of this study was to characterize the trophic position of lake sturgeon in the context of an invasive species dominated food web and to describe their movement patterns and residency within the lower Niagara River. Stomach content analysis and stable nitrogen (δ¹⁵N) and carbon (δ¹³C) isotope analysis of tissue was used …

Dopamine Levels In The Brain Of Rat Models Of Human Rheumatoid Arthritis, Amelia Stinson

Theses & Dissertations

Research Focus. Rheumatoid arthritis (RA) is a chronic, debilitating, autoimmune disease that causes the destruction of bone tissue and the articular structures of joints. At least 30% of RA patient populations have cognitive impairment. Acidic dopamine (DA) is the principal neuroimmunotransmitter that links the central nervous system and peripheral nervous system together. The aim of the present study was to determine the levels of DA and its two acidic metabolites: 3,4-dihydroxyphenylacetic acid (DOPAC) and homovanillic acid (HVA) in arthritic induced rats, and whether their levels vary across four different parts of the brain: amygdala (AMG), front cerebral cortex (CX), hippocampus …

Identifying Genetic Factors Influencing Sperm Mobility Phenotype In Chicken Using Genome Wide Association Studies, Primordial Germ Cell Transplantation, And Rnaseq., Sohita Ojha

Graduate Theses and Dissertations

Sperm mobility is a major determinant of male fertility in chicken. In spite of low heritability of reproductive traits, sperm mobility has high heritability index which suggests presence of quantitative trait loci (QTLs) governing the trait. Our research focused on three objectives: i) to identify the QTLs affecting low mobility phenotype in chicken, ii) to understand the impact of Sertoli-cells and germ cells interactions in influencing the mobility phenotype and iii) to identify the genes and gene networks differentially expressed in male and female PGCs. To detect the QTLs, genome wide association studies (GWAS) was conducted which revealed the presence …

Lactational Performance And Energy Partitioning Of Dairy Cows Supplemented With N-Acetyl-L-Methionine During Mid To Late Lactation, Tyson George Grisenti

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The N-acetyl-L-methionine (NALM) molecule is a methionine (Met) derivative produced via acetylation of the L-Met α-amino group with an N-acetyl group. This molecule has been shown to be bioavailable and capable of fulfilling the dietary requirement for Met in animals and humans. The current experiment was conducted to test a hypothesis that lactating dairy cows fed with NALM would increase milk production by increasing N and energy utilization efficiencies in a dose dependent manner. Eight multiparous Holstein cows that were mid lactation (124 ± 13 days-in-milk) with similar milk production were used in a 4 x …

Genetic Diversity Of Kemp's Ridley (Lepidochelys Kempii) Sea Turtles On South Padre Island, Texas, Hilary R. Frandsen

Theses and Dissertations

In 2010, researchers observed an abrupt decline in nesting numbers of the Kemp’s ridley following 35 years of positive growth. In wake of this halt, and subsequent lowered nesting averages, evaluation of the genetic diversity of remaining individuals is relevant to investigate the plausibility of a genetic bottleneck, and determine the reproductive stability of the Kemp’s ridley.

Here, I evaluate the genetic diversity within a sampling of the Kemp’s ridleys on South Padre Island using two methods: targeting the mitochondrial control region and targeting the complete mitochondrial genome. Opportunistic samples were donated from juvenile ridleys on the East coast, and …

A Comparative Life Cycle Assessment Of Nutritionally Equivalent Meals With And Without Pork, Kelli Young

Graduate Theses and Dissertations

The following report details a life cycle assessment of several dietary and meal scenarios with and without pork. The goal of the LCA was to identify the impacts of greenhouse gas emissions, water use, and land use of pork containing and porkless diets and meal plans in a field-to-fork analysis. The dietary and meal plan scenarios are iso-caloric meaning they contain the same number of calories. The first set of diets is based on a USDA consumption pattern, 2000 kcal per day. This diet was used to create three other dietary scenarios with and without pork. The USDA recommended food …

Coping With Drought In Beef Cattle Production: Innovation Through Optimal Warm-Season Forage Systems, Katelynn Elizabeth Zechiel

Masters Theses

Drought conditions have had detrimental effects on beef cattle production in the southeastern states where forages are the primary source of feed for livestock. Many southeastern states lie within the fescue-belt, where tall fescue is the predominant livestock forage. Tall fescue is a cool-season (CS) grass that thrives in the cooler temperatures of spring and fall, becoming semi-dormant during peak summer temperatures and again in winter. Conversely, warm-season (WS) forage species increase in production during the summer months and exhibit drought tolerant qualities, making them a viable summer forage option to complement tall fescue for beef cattle producers. The primary …

Time Series Forecast Analysis In Wholesale Broiler Markets, Christopher R. Sims

Graduate Theses and Dissertations

In 2016 the chicken industry provided nearly 1.2 million jobs, 68 billion dollars in wages, 313 billion dollars in economic activity and 24 billion dollars in government revenue (John Dunham & Associates, Inc., 2016). Broiler production has changed dramatically from the early 90’s to the turn of the 21st century. Technological advancements, continuous improvements, production efficiencies and industry changes have made the industry the global market it is today. The poultry industry is an extremely volatile market with prices constantly fluctuating in response to input price volatility and demand and supply changes. These changes are often driven by world economic …

Utilizing Accelerometer Telemetry Tags To Compare Red Snapper (Lutjanus Campechanus) Behavior On Artificial And Natural Reefs, Ethan T. Getz

Theses and Dissertations

The Texas Parks and Wildlife Department Artificial Reef Program has deployed over 2,800 hectares of reefing area composed of petroleum platforms, steel vessels, and concrete. Artificial reefs have been shown to support many important reef fishes such as red snapper (Lutjanus campechanus), however, few studies have compared fish behavior on artificial reefs and natural habitats. We examined activity levels, behavioral patterns and habitat preferences of red snapper over natural reefs, oil platforms and submerged ships. Telemetry tags (Sonotronics model MTT) with tri-axial acceleration range, average depth, and average temperature were programmed to optimize data collection and tag life. …

Effect Of Land Use Change On South Texas Bats, Katharine Leigh Jones

Theses and Dissertations

Bats were acoustically recorded in South Texas over the summer of 2017. Nine species of bats were recorded and identified.

The Spatial Ecology And Microhabitat Selection Of The Pygmy Rattlesnake (Sistrurus Miliarius) In Southwestern Missouri, Dylan Wallace Maag

MSU Graduate Theses

Despite a wide distribution throughout the southeastern United States, pygmy rattlesnakes (Sistrurus miliarius) have received less research attention than many other rattlesnake species. I captured a total of 33 S. miliarius at the Drury-Mincy Conservation Area (DMCA) and retained 14 large individuals (mostly gravid females) for a radio telemetry study. Snakes were primarily captured during evening road driving surveys and were encountered rarely with any other sampling technique. Sistrurus miliarius are widespread at DMCA where they were encountered in forest, savanna, and glade habitats. Snakes selected microhabitats with more vegetative cover and tree canopy closure than random sites …

The Effect Of Weight Carried And Time Ridden On Back Pain In Horses Ridden During Horse Shows As Determined By Pressure Algometry, Meghan Louise Mothershead

MSU Graduate Theses

The objective of this study was to determine the effects of weight carried (rider + tack) on back pain in horses used in riding classes and a series of intercollegiate horse shows. Sixteen school horses (431-649 kg) were ridden consistently over a semester. Cross sectional images were used to measure width and put in a mapping program to figure total area. Back pain scores were determined using a pressure algometer on predetermined points over the horses back. Pain scores were recorded over five periods, including 28 d into the semester (P1) and during the show period on three consecutive Fridays …

Effect Of Endophyte-Infected Tall Fescue Toxins On Growth Performance And The Microbial Community In The Rumen And Feces In Pregnant Ewes, Saleh Alrashedi

Graduate Theses and Dissertations

Fifty pregnant Katahdin ewes grazed pastures with high (HE) and moderate (MI) levels of Neotyphodium coenophialum-infected tall fescue between February 1 and May 24 to determine the impacts of tall fescue toxins on body weight (BW), body condition scores, FAMACHA scores, fecal egg counts (FEC), and serum Cu, Zn, prolactin, and non-esterified fatty acids (NEFA). Within those 50 ewes, 15 were selected randomly for fecal and rumen sampling to assess the impacts of HE and MI on the microbiome community. Total bacterial DNA was extracted from rumen and fecal samples, and alpha and beta diversities were used to relate microbiome …

Experimental Approaches To Understand And Control Salmonella Infection In Poultry, Yichao Yang

Graduate Theses and Dissertations

Salmonella is a major foodborne pathogen around the world and chickens are the major reservoir to transmit Salmonella into the human food chain. For decreasing the infection of Salmonella, we developed six attenuated live vaccines based on Salmonella Enteritidis (SE) and Typhimurium (ST) for testing the cross-serovar and cross-serogroup protection from the challenge of Salmonella Heidelberg and Campylobacter jejuni. One of the constructed vaccine strain showed ability to protect against challenge from Salmonella Heidelberg. Even though some preventive approaches are able to decrease Salmonella colonization in the gastrointestinal tract of chickens or other farm animals, Salmonella transmission mechanisms remain unclear. …

The Effect Of Pet Ownership On Esl Students’ U.S. Academic Success, Emily Benjumea

Animal Science Undergraduate Honors Theses

This study examines the academic success of English as a second language (ESL) students based on ownership of and/or relationship with a pet, due to pets’ ability to benefit and support their owners. A survey was compiled for middle school, ESL students in the Fayetteville, Arkansas school district. The goal of the study was to discover if pet ownership has an effect on ESL student academic success in terms of the academic, social, and emotional aspects of school. It was found that pets aid in some academic aspects of school but more so in the social and emotional aspects such …

A Study Of The Prevalence Of Gastrointestinal Nematodes In Goats Obtained From Northwest Arkansas, Christine Rose Weingartz

Graduate Theses and Dissertations

Parasitic helminths have always been an issue in small ruminant production; pathogens that pose great negative impact on goat health and productivity. Insufficient work has been done to document the prevalence of parasitic helminths in the United States, especially in the south and southeast, where the largest goat populations are found.

The aim of this study was to survey the prevalence of infections by gastrointestinal nematodes in goats in Northwestern Arkansas. Gastrointestinal tracts were examined from 41 goats of various locations around Northwest Arkansas. Worm species were identified and population burdens were determined. Coprology was correlated with the actual worm …

Development Of An Animal Model For Enterovirus For Evaluation D68 For Screening Of Antiviral Therapies, W. Joseph Evans

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Enterovirus D68 (EV-D68) virus has become more prevalent over the last 15 to 20 years. EV-D68 attacks the respiratory system and can cause severe disease in individuals who have underlying respiratory problems. There have also been reports of individuals with EV-D68 showing signs of neurological system problems and acute flaccid paralysis. Because of the increase in patients with EV-D68 and also the potential for neurological disease, an animal model is needed to study the disease and to evaluate experimental therapies for EV-D68 infection.

To develop the animal model, 4-week old AG129 mice that lack alpha and beta interferon receptors, making …

Evaluating Social Network Dynamics Of Bigg’S Killer Whales (Orcinus Orca) And Vessel Traffic Within A Transboundary Region: Implications For Conservation Management, Courtney Smith

Dissertations

The social lives of animals are defined by group dynamics based on the nature and strength of associations and movements between individuals, often resulting in highly complex and interconnected social networks. However, understanding of how environmental variables may shape this structure is poorly understood. Within the inland waters of Washington State and southern Vancouver Island, British Columbia, mammal-eating Bigg’s (transient) killer whales occur in relatively small, but stable social groups. Group size and occurrence in recent years has increased, coinciding with a growing whale watching industry. Given the central importance of the social network within killer whale population dynamics, such …

Escorting Of Mother-Calf Pairs Of Humpback Whales (Megaptera Novaeangliae) In The Colombian Pacific During The Breeding Season, Natalia Botero Acosta

Dissertations

Humpback whales (Megaptera novaeangliae) belonging to the “breeding G-stock” annually migrate from the Antarctic Peninsula and southern Chile to the southeastern Pacific to reproduce. Associations between mother-calf pairs and escorts were examined in the Gulf of Tribugá, northern Colombian Pacific, using photo-identification and behavioral/spatial sampling. Research hypotheses included: 1. The association between cows and escorts is short-lived, consistent with a male reproductive strategy, 2. The presence of escort(s) elicits a behavioral response from mother-calf pairs and, 3. The patterns of spatial distribution reflect the spatial segregation of maternal females. Groups were classified as mother-calf pairs (Mc), mother, calf …

Bat Population Status And Roost Selection Of Tri-Colored Bats In The Great Smoky Mountains National Park In The Era Of White-Nose Syndrome, Grace Marie Carpenter

Masters Theses

The ongoing spread of white-nose syndrome is causing devastating declines range-wide for certain North American bat species. Baseline population data that would help mangers monitor bat populations in the face of WNS is lacking. Likewise, knowledge of summer roosts, a limiting resource for tri-colored bats (Perimyotis subflavus), a species threatened by WNS, is lacking in the southern portion of their range. In our study, we investigated the effect that WNS has had on a population of tricolored bats in the Great Smoky Mountains National Park, TN-NC. We also characterized summer roosts for the species at the microhabitat and …