Life Sciences Commons^™

Do Flame Retardants Promote Vitamin D Deficiency?, Audrey Petteruti

Honors Theses and Capstones

Vitamin D deficiency in the Unites States has become more prevalent in recent years. Research has shown that environmental chemicals such as flame-retardants induce hepatic enzymes in the cytochrome P450 family such as CYP24 and CYP3A that are important in vitamin D metabolism. To determine if exposure to one class of flame-retardants known as polybrominated diethyl ethers (PBDEs) promotes vitamin D deficiency, 15 rats consumed a diet marginally deficient in Vitamin D - 85 IU Vitamin D/kg diet - for 56 days. On day 28 of the experiment, 7 rats were gavaged daily with 7 mg/kg BW PBDEs and 8 …

Drought Sensitivity Of Slash Pine And Longleaf Pine Deduced By Tree Ring Analysis, Conor Madison

Honors Theses and Capstones

Annual tree rings give us the opportunity to investigate the adaptation of trees to climate and environmental changes over a long period of time. In particular, the physical characteristics of each ring (width and earlywood and late wood differentiation) can be used to reconstruct past environment conditions. Physiological responses of trees will be evaluated for two conifers species, i.e. Longleaf pine and Slash pine, giving the opportunity to compare the two species and understand how each species (Longleaf pine and Slash pine) adapt their water use to thrive in such extreme environments. Growth data will then be related to the …

Effects Of Audible Human Disturbance On Koala (Phascolarctos Cinereus) Behavior In Queensland, Australia And Implications For Management, Galina Eugenia Kinsella

Honors Theses and Capstones

As the growing human population continues to encroach on wildlife habitat, species are forced to adapt in order to survive. In addition to causing habitat loss, human presence can create more subtle disturbances, such as noise pollution, that disrupt wildlife behavior. Adapting to human presence is particularly difficult for species with specialized resource needs or low mobility. The koala (Phascolarctos cinereus), a national icon of Australia, meets both these criteria. Koalas were once abundant throughout Australia, but are now classified as “threatened.” Habitat loss is at least partly responsible for this decline, but anthropogenic noise may also be …

How Do Various Maize Crop Models Vary In Their Responses To Climate Change Factors?, Simon Bassu, Nadine Brisson, Jean-Louis Durand, Kenneth J. Boote, Jon Lizaso, James Jones, Cynthia Rosenzweig, Alex Ruane, Myriam Adam, Christian Baron, Bruno Basso, Christian Biernath, Hendrick Boogaard, Sjaak Conijn, Marc Corbeels, Delphine Deryng, Giacomo De Sanctis, Sebastian Gayler, Patricio Grassini, Jerry Hatfield, Steven Hoek, Cesar Izaurralde, Raymond Jongschaap, Armen Kemanian, Christian Kersebaum, Soo-Hyung Kim, Naresh Kumar, David Makowski, Christoph Muller, Claas Nendel, Eckart Priesack, Maria Virinia Pravia, Federico Sau, Iurii Shcherbak, Fulu Tao, Edmar Teixeira, Dennis Timlin, Katharina Waha

Department of Agronomy and Horticulture: Faculty Publications

Potential consequences of climate change on crop production can be studied using mechanistic crop simulation models. While a broad variety of maize simulation models exist, it is not known whether different models diverge on grain yield responses to changes in climatic factors, or whether they agree in their general trends related to phenology, growth, and yield. With the goal of analyzing the sensitivity of simulated yields to changes in temperature and atmospheric carbon dioxide concentrations [CO2], we present the largest maize crop model intercomparison to date, including 23 different models. These models were evaluated for four locations representing a wide …

Drought-Induced Woody Plant Mortality In An Encroached Semi-Arid Savanna Depends On Topoedaphic Factors And Land Management, Dirac L. Twidwell Jr, Carissa L. Wonkka, Charles A. Taylor, Chris B. Zou, Jeremiah J. Twidwell, William E. Rogers

Department of Agronomy and Horticulture: Faculty Publications

Questions: How do recent patterns of drought-induced woody plant mortality in Texas semi-arid savanna compare to the extended drought of the 1950s? Does the relative composition of the woody plant community shift ubiquitously across the landscape following woody plant mortality and dieback or are shifts dependent on differences among species, soils, land use and plant demography?

Location: Texas Agrilife Research Station, Sonora, Texas, USA (30.1° N 100.3° W).

Methods: Following an exceptional drought from 1951 to 1957, a study was conducted to quantify rates of mortality for various woody plant species. In 2011, we repeated this study within three long-term …

Identification Of Differentially Expressed Genes Between Sorghum Genotypes With Contrasting Nitrogen Stress Tolerance By Genome-Wide Transcriptional Profiling, Malleswari Gelli, Yongchao Duo, Anji Reddy Konda, Chi Zhang, David R. Holding, Ismail M. Dweikat

Department of Agronomy and Horticulture: Faculty Publications

Background: Sorghum is an important cereal crop, which requires large quantities of nitrogen fertilizer for achieving commercial yields. Identification of the genes responsible for low-N tolerance in sorghum will facilitate understanding of the molecular mechanisms of low-N tolerance, and also facilitate the genetic improvement of sorghum through marker-assisted selection or gene transformation. In this study we compared the transcriptomes of root tissues from seven sorghum genotypes having differential response to low-N stress.

Results: Illumina RNA-sequencing detected several common differentially expressed genes (DEGs) between four low-N tolerant sorghum genotypes (San Chi San, China17, KS78 and high-NUE bulk) and three …

Global Changes In Mineral Transporters In Tetraploid Switchgrasses (Panicum Virgatum L.), Nathan A. Palmer, Aaron J. Saathoff, Brian M. Waters, Teresa Donze, Tiffany M. Heng-Moss, Paul Twigg, Christian M. Tobias, Gautam Sarath

Department of Agronomy and Horticulture: Faculty Publications

Switchgrass (Panicum virgatum L) is a perennial, C4 grass with great potential as a biofuel crop. An in-depth understanding of the mechanisms that control mineral uptake, distribution, and remobilization will benefit sustainable production. Nutrients are mobilized from aerial portions to below-ground crowns and rhizomes as a natural accompaniment to above-ground senescence post seed-set. Mineral uptake and remobilization is dependent on transporters, however, little if any information is available about the specific transporters that are needed and how their relative expression changes over a growing season.Using well-defined classes of mineral transporters, we identified 520 genes belonging to 40 different transporter …

Grazing Management Effect On Micro- And Macro- Scale Fate Of C And N In Rangelands, Martha Mamo, Jeff Bradshaw, Richard Ferguson, Kent M. Eskridge, John A. Guretzky, Karla Jenkins, Walter H. Schacht, Jerry Volesky, Sean Whipple, Anita Wingeyer, Haishun Yang

Department of Agronomy and Horticulture: Faculty Publications

No abstract provided.

Quantitative Proteomic Analysis Of The Salmonella-Lettuce Interaction, Yuping Zhang, Renu Nandakumar, Shannon L. Bartelt-Hunt, Daniel D. Snow, Laurie Hodges, Xu Li

Department of Agronomy and Horticulture: Faculty Publications

Human pathogens can internalize food crops through root and surface uptake and persist inside crop plants. The goal of the study was to elucidate the global modulation of bacteria and plant protein expression after Salmonella internalizes lettuce. A quantitative proteomic approach was used to analyse the protein expression of Salmonella enterica serovar Infantis and lettuce cultivar Green Salad Bowl 24 h after infiltrating S. Infantis into lettuce leaves. Among the 50 differentially expressed proteins identified by comparing internalized S. Infantis against S. Infantis grown in Luria Broth, proteins involved in glycolysis were down-regulated, while one protein involved in ascorbate …

Switchgrass (Panicum Virgatum L) Flag Leaf Transcriptomes Reveal Molecular Signatures Of Leaf Development, Senescence, And Mineral Dynamics, Nathan A. Palmer, Teresa Donze-Reiner, David Horvath, Tiffany Heng-Moss, Brian M. Waters, Christian M. Tobias, Gautam Sarath

Department of Agronomy and Horticulture: Faculty Publications

Switchgrass flag leaves can be expected to be a source of carbon to the plant, and its senescence is likely to impact the remobilization of nutrients from the shoots to the rhizomes. However, many genes have not been assigned a function in specific stages of leaf development. Here, we characterized gene expression in flag leaves over their development. By merging changes in leaf chlorophyll and the expression of genes for chlorophyll biosynthesis and degradation, a four-phase molecular roadmap for switchgrass flag leaf ontogeny was developed. Genes associated with early leaf development were up-regulated in phase 1. Phase 2 leaves had …

Genotyping By Sequencing For Genomic Prediction In A Soybean Breeding Population, Diego Jarquin, Kyle Kocak, Luis Posadas, Katie Hyma, Joseph Jedlicka, George L. Graef, Aaron Lorenz

Department of Agronomy and Horticulture: Faculty Publications

Background: Advances in genotyping technology, such as genotyping by sequencing (GBS), are making genomic prediction more attractive to reduce breeding cycle times and costs associated with phenotyping. Genomic prediction and selection has been studied in several crop species, but no reports exist in soybean. The objectives of this study were (i) evaluate prospects for genomic selection using GBS in a typical soybean breeding program and (ii) evaluate the effect of GBS marker selection and imputation on genomic prediction accuracy. To achieve these objectives, a set of soybean lines sampled from the University of Nebraska Soybean Breeding Program were genotyped using …

Detection And Confirmation Of Quantitative Trait Loci For Soybean Seed Isoflavones, Christopher J. Smallwood, Catherine N. Nyinyi, Dean A. Kopsell, Carl E. Sams, Dennis R. West, Pengyin Chen, Stella K. Kantartzi, P. B. Cregan, D. L. Hyten, Vincent R. Pantalone

Department of Agronomy and Horticulture: Faculty Publications

Interest in soybean [Glycine max (L.) Merr.] isoflavones has increased in recent years owing to numerous reported health benefits. Consequently, quantitative trait loci (QTL) detection for marker-assisted breeding for isoflavones is being examined for genetic gains. This study sought to detect QTL for soybean isoflavones in a population of 274 recombinant inbred lines derived from a cross between ‘Essex’ and ‘Williams 82’ that were subdivided and tested by maturity (early, mid, and late). The field tests were conducted in three environments in 2009 (Knoxville, TN; Harrisburg, IL; and Stuttgart, AR). The population was genotyped with 480 polymorphic single nucleotide …

A Reference Genome For Common Bean And Genome-Wide Analysis Of Dual Domestications, Jeremy Schmutz, Phillip E. Mcclean, Sujan Mamidi, G. Albert Wu, Steven B. Cannon, Jane Grimwood, Jerry Jenkins, Shengqiang Shu, Qijian Song, Carolina Chavarro, Mirayda Torres-Torres, Valerie Geffroy, Samira Mafi Moghaddam, Dongying Gao, Brian Abernathy, Kerrie Barry, Matthew Blair, Mark A. Brick, Mansi Chovatia, Paul Gepts, David M. Goodstein, Michael Gonzales, Uffe Hellsten, D. L. Hyten, Gaofeng Jia, James D. Kelly, Dave Kudrna, Rian Lee, Manon M.S. Richard, Phillip N. Miklas, Juan M. Osorno, Josiane Rodrigues, Vincent Thareau, Carlos A. Urrea Florez, Mei Wang, Yeisoo Yu, Ming Zhang, Rod A. Wing, P. B. Cregan, Daniel S. Rokhsar, Scott A. Jackson

Department of Agronomy and Horticulture: Faculty Publications

Common bean (Phaseolus vulgaris L.) is the most important grain legume for human consumption and has a role in sustainable agriculture owing to its ability to fix atmospheric nitrogen. We assembled 473 Mb of the 587-Mb genome and genetically anchored 98% of this sequence in 11 chromosome-scale pseudomolecules. We compared the genome for the common bean against the soybean genome to find changes in soybean resulting from polyploidy. Using resequencing of 60 wild individuals and 100 landraces from the genetically differentiated Mesoamerican and Andean gene pools, we confirmed 2 independent domestications from genetic pools that diverged before human colonization. …

The Interaction Of Climate Change, Land Cover, And Political Representation In The Usa, Brady W. Allred, Dirac L. Twidwell, Samuel D. Fuhlendorf

Department of Agronomy and Horticulture: Faculty Publications

The difficulties in tackling climate change are inherently complex and primarily centered on political and social values. This is evident in the United States where political divisions and polarizations are fundamental barriers to advancing national policies, which in turn hinder international agreements, mitigation, and adaptation. Within the United States, the vast majority of agricultural and natural resource lands are projected to incur significant climate departures and are represented by the Republican Party. The resources and economic sectors that will be directly affected by climate change are represented by national leadership that is unlikely to accept policies to prevent or adapt …

Arabidopsis Lipins, Pdat1 Acyltransferase, And Sdp1 Triacylglycerol Lipase Synergistically Direct Fatty Acids Toward Β-Oxidation, Thereby Maintaining Membrane Lipid Homeostasis, Jilian Fan, Chengshi Yan, Rebecca Roston, John Shanklin, Changcheng Xu

Department of Agronomy and Horticulture: Faculty Publications

Triacylglycerol (TAG) metabolism is a key aspect of intracellular lipid homeostasis in yeast and mammals, but its role in vegetative tissues of plants remains poorly defined. We previously reported that PHOSPHOLIPID:DIACYLGLYCEROL ACYLTRANSFERASE1 (PDAT1) is crucial for diverting fatty acids (FAs) from membrane lipid synthesis to TAG and thereby protecting against FA-induced cell death in leaves. Here, we show that overexpression of PDAT1 enhances the turnover of FAs in leaf lipids. Using the trigalactosyldiacylglycerol1-1 (tgd1-1) mutant, which displays substantially enhanced PDAT1- mediated TAG synthesis, we demonstrate that disruption of SUGAR-DEPENDENT1 (SDP1) TAG lipase or PEROXISOMAL TRANSPORTER1 (PXA1) severely decreases …

Nonsyntenic Genes Drive Highly Dynamic Complementation Of Gene Expression In Maize Hybrids, Anja Paschold, Nick B. Larson, Caroline Marcon, James C. Schnable, Cheng-Ting Yeh, Christa Lanz, Dan Nettleton, Hans-Peter Piepho, Patrick S. Schnable, Frank Hochholdinger

Department of Agronomy and Horticulture: Faculty Publications

Maize (Zea mays) displays an exceptional level of structural genomic diversity, which is likely unique among higher eukaryotes. In this study, we surveyed how the genetic divergence of two maize inbred lines affects the transcriptomic landscape in four different primary root tissues of their F1-hybrid progeny. An extreme instance of complementation was frequently observed: genes that were expressed in only one parent but in both reciprocal hybrids. This single-parent expression (SPE) pattern was detected for 2341 genes with up to 1287 SPE patterns per tissue. As a consequence, the number of active genes in hybrids exceeded that of …

Dt2 Is A Gain-Of-Function Mads-Domain Factor Gene That Specifies Semideterminacy In Soybean, Jieqing Ping, Yunfeng Liu, Lianjun Sun, Meixia Zhao, Yinghui Li, Zongxiang Tang, Hanh Nguyen, Zhixi Tian, Lijuan Qiu, Randall L. Nelson, Thomas E. Clemente, James Specht, Jianxin Ma

Department of Agronomy and Horticulture: Faculty Publications

Similar to Arabidopsis thaliana, the wild soybeans (Glycine soja) and many cultivars exhibit indeterminate stem growth specified by the shoot identity gene Dt1, the functional counterpart of Arabidopsis TERMINAL FLOWER1 (TFL1). Mutations in TFL1 and Dt1 both result in the shoot apical meristem (SAM) switching from vegetative to reproductive state to initiate terminal flowering and thus produce determinate stems. A second soybean gene (Dt2) regulating stem growth was identified, which, in the presence of Dt1, produces semideterminate plants with terminal racemes similar to those observed in determinate plants. Here, we report positional …

Nebraska’S Groundwater Legacy: Nitrate Contamination Beneath Irrigated Cropland, Mary E. Exner Spaulding, Aaron J. Hirsh, Roy F. Spalding

Department of Agronomy and Horticulture: Faculty Publications

A 31 year record of ~44,000 nitrate analyses in ~11,500 irrigation wells was utilized to depict the decadal expansion of groundwater nitrate contamination (N>10 mg/L) in the irrigated corn-growing areas of eastern and central Nebraska and analyze long-term nitrate concentration trends in 17 management areas (MAs) subject to N fertilizer and budgeting requirements. The 1.3 M contaminated hectares were characterized by irrigation method, soil drainage, and vadose zone thickness and lithology. The areal extent and growth of contaminated groundwater in two predominately sprinkler-irrigated areas was only ~20% smaller beneath well-drained silt loams with thick clayey-silt unsaturated layers and unsaturated …

Multilocation Corn Stover Harvest Effects On Crop Yields And Nutrient Removal, Douglas L. Karlen, Stuart J. Birrell, Jane M.F. Johnson, Shannon L. Osborne, Thomas E. Schumacher, Gary E. Varvel, Richard B. Ferguson, Jeff M. Novak, James R. Frederick, John M. Baker, John A. Lamb, Paul R. Alder, Greg W. Roth, Emerson D. Nafziger

Department of Agronomy and Horticulture: Faculty Publications

Corn (Zea mays L.) stover was identified as an important feedstock for cellulosic bioenergy production because of the extensive area upon which the crop is already grown. This report summarizes 239 site-years of field research examining effects of zero, moderate, and high stover removal rates at 36 sites in seven different states. Grain and stover yields from all sites as well as N, P, and K removal from 28 sites are summarized for nine longitude and six latitude bands, two tillage practices (conventional vs no tillage), two stoverharvest methods (machine vs calculated), and two crop rotations {continuous corn (maize) …

Constructing A Block And Fence Growing Bench For Use With A Capillary Mat Irrigation System For Greenhouse Plant Production, Stacy A. Adams, Ellen T. Paparozzi

Department of Agronomy and Horticulture: Faculty Publications

Plant health and productivity of greenhouse grown plants are improved when grown on raised benches where they can have optimal environmental conditions. Often seasonal plant growers set plants directly on the greenhouse floor which is less than optimum due to temperature stratification, cold drafts, poor air movement, and wet conditions. This publication describes how to construct simple and economical growing benches and a irrigation system useful for the specialty crop grower.

Do Cover Crops Increase Or Decrease Nitrous Oxide Emissions? A Meta-Analysis, Andrea D. Basche, F. E. Miguez, T. C. Kaspar, M. J. Castellano

Department of Agronomy and Horticulture: Faculty Publications

There are many environmental benefits to incorporating cover crops into crop rotations, such as their potential to decrease soil erosion, reduce nitrate (NO₃) leaching, and increase soil organic matter. Some of these benefits impact other agroecosystem processes, such as greenhouse gas emissions. In particular, there is not a consensus in the literature regarding the effect of cover crops on nitrous oxide (N₂O) emissions. Compared to site-specific studies, meta-analysis can provide a more general investigation into these effects. Twenty-six peer-reviewed articles including 106 observations of cover crop effects on N₂O emissions from the soil surface …

Challenges And Opportunities In Transdisciplinary Science: The Experience Of Next Generation Scientists In An Agriculture And Climate Research Collaboration, Andrea D. Basche, Gabrielle E. Roesch-Mcnally, Lindsay A. Pease, Christopher D. Eidson, Guy Bou Lahdou, Mike W. Dunbar, Trevor J. Frank, Laura Frescoln, Lei Gu, Ryan Nagelkirk, Jose Pantoja, Adam K. Wilke

Department of Agronomy and Horticulture: Faculty Publications

Agriculture in the twenty-first century faces unprecedented challenges from increasing climate variability to growing demands on natural resources to globalizing economic markets. These emerging agricultural issues, spanning both human and natural dimensions, are uniquely formulated, exceedingly complex, and difficult to address within existing disciplinary domains (Eigenbrode et al. 2007; Reganold et al. 2011; Foley et al. 2005; Hansen et al. 2013). Therefore, the next generation of scientists working on these issues must not only be highly trained within a disciplinary context but must also have the capacity to collaborate with others to solve systems-level problems.

The Carbon-Nitrogen Balance Of The Nodule And Its Regulation Under Elevated Carbon Dioxide Concentration, Marc Libault

Department of Agronomy and Horticulture: Faculty Publications

Legumes have developed a unique way to interact with bacteria: in addition to preventing infection from pathogenic bacteria like any other plant, legumes also developed a mutualistic symbiotic relationship with one gender of soil bacteria: rhizobium. This interaction leads to the development of a new root organ, the nodule, where the differentiated bacteria fix for the plant the atmospheric dinitrogen (atmN₂). In exchange, the symbiont will benefit from a permanent source of carbon compounds, products of the photosynthesis. The substantial amounts of fixed carbon dioxide dedicated to the symbiont imposed to the plant a tight regulation of the …

ประชาคมฮาร์แพกทิคอยด์โคพีพอดบริเวณป่าชายเลนชุมชนบ้านทุ่งตะเซะ จังหวัดตรัง, ปัทมาภรณ์ ชัยมัง

Chulalongkorn University Theses and Dissertations (Chula ETD)

การศึกษาประชาคมฮาร์แพกทิคอยด์โคพีพอดบริเวณป่าชายเลนชุมชนบ้านทุ่งตะเซะ จังหวัดตรัง ซึ่งเป็นพื้นที่ป่าชายเลนที่อุดมสมบูรณ์แบ่งพื้นที่ศึกษาออกเป็น 7 สถานี มี 3 พื้นที่หลัก คือ พื้นที่ป่าชายเลนด้านในที่ติดแผ่นดินและบริเวณคลองด้านใน พื้นที่ป่าชายเลนด้านนอกที่ติดทะเลในบริเวณคลองเกาะเคี่ยม และบริเวณเกาะกลางที่อยู่ตรงข้ามศูนย์อนุรักษ์หอยปะของชุมชน ทำการเก็บตัวอย่าง 4 ครั้งคือ ช่วงก่อนการปลูกป่าซึ่งเป็นการปลูกเสริมและฟื้นฟูในเดือนตุลาคม 2553 (ฤดูฝน) หลังจากปลูกป่า 6 เดือนในเดือนเมษายน 2554 (ฤดูแล้ง) หลังจากปลูกป่า 1 ปี 6 เดือนในเดือนเมษายน 2555 (ฤดูแล้ง) และหลังจากปลูกป่า 2 ปี 6 เดือนในเดือนพฤษภาคม 2556 (ฤดูแล้ง) พบสัตว์ทะเลหน้าดินเล็กทั้งหมด พบทั้งสิ้น 12 ไฟลัม แบ่งออกเป็น 29 กลุ่ม สัตว์ทะเลหน้าดินขนาดเล็กที่พบเป็นกลุ่มเด่นคือ Nematodes, Foraminiferans และ Harpacticoid copepods ความหนาแน่นของสัตว์ทะเล หน้าดินขนาดเล็กในบริเวณป่าชายเลนตอนในและตอนนอกของป่าชายเลนชุมชนบ้านทุ่งตะเซะ จังหวัดตรัง ทั้งในช่วงก่อนและหลังปลูกป่า 2 ปี 6 เดือน มีค่าอยู่ในเกณฑ์สูง ซึ่งมีความหนาแน่นสูงกว่า 500 ตัวต่อ 10 ตารางเซนติเมตร แสดงว่าบริเวณนี้เป็นป่าที่มีความอุดมสมบูรณ์ และพบว่าความหนาแน่น มีค่าสูงขึ้นภายหลังจากการปลูกป่า ฮาร์แพกทิคอยด์โคพีพอดในบริเวณป่าชายเลนชุมชนบ้านทุ่งตะเซะ จังหวัดตรังพบทั้งสิ้น 8 วงศ์ ได้แก่ Cletodidae, Darcythompsoniidae, Ectinosomatidae, Harpacticidae, Metidae, Miraciidae, Thalestridae และ Tisbidae โดยพบวงศ์ Cletodidae เป็นกลุ่มเด่น ความหนาแน่นของฮาร์แพกทิคอยด์โคพีพอดบริเวณป่าชายเลนตอนในมีค่าอยู่ในช่วง 9-185 ตัวต่อ 10 ตารางเซนติเมตร ส่วนป่าชายเลนตอนนอกมีความหนาแน่นอยู่ในช่วง 22-169 ตัวต่อ 10 ตารางเซนติเมตร หลังจากปลูกป่าพบว่าความหนาแน่นมีค่าสูงขึ้นเกือบทุกบริเวณที่ทำการศึกษา ยกเว้นบริเวณป่าชายเลนตอนในในสถานีป่าไม้ตะบูนที่ความหนาแน่นช่วงก่อนและหลังปลูกป่ามีค่าใกล้เคียงกัน การศึกษาการกินอาหารของฮาร์แพกทิคอยด์โคพีพอดบริเวณป่าชายเลนชุมชนบ้านทุ่งตะเซะ สามารถจัดกลุ่มการกินอาหารของฮาร์แพกทิคอยด์โคพีพอดได้ทั้งสิ้น 5 กลุ่ม …

Synthesis And Evaluation Of Cytotoxic Activity Of Substituted N-(9-Oxo-9h-Xanthen-4-Yl) Benzenesulfonamides, Somayeh Motavallizadeh, Asal Fallah-Tafti, Saeedeh Maleki, Amir Nasrolahi Shirazi, Mahboobeh Pordeli, Maliheh Safavi, Sussan Kabudanian Ardestani, Shaaban Asd, Rakesh Tiwari, Donghoon Oh, Abbas Shafiee, Alireza Foroumadi, Keykavous Parang, Tahmineh Akbarzadeh

Pharmacy Faculty Articles and Research

Several novel N-(9-oxo-9H-xanthen-4-yl)benzenesulfonamides derivatives were prepared as potential antiproliferative agents. The in vitro antiproliferative activity of the synthesized compounds was investigated against a panel of tumor cell lines including breast cancer cell lines (MDA-MB-231, T-47D) and neuroblastoma cell line (SK-N-MC) using MTT colorimetric assay. Etoposide, a well-known anticancer drug, was used as a positive standard drug. Among synthesized compounds, 4-methoxy-N-(9-oxo-9H-xanthen-4-yl)benzenesulfonamide (5i) showed the highest antiproliferative activity against MDA-MB-231, T-47D, and SK-N-MC cells. Furthermore, pentafluoro derivatives 5a and 6a exhibited higher antiproliferative activity than doxorubicin against human leukemia cell line (CCRF-CEM) and breast adenocarcinoma (MDA-MB-468) cells. Structure-activity relationship studies revealed that …

Synthesis And Evaluation Of Antiproliferative Activity Of Substituted N-(9-Oxo-9h-Xanthen-4-Yl)Benzenesulfonamides, Somayeh Motavallizadeh, Asal Fallah-Tafti, Saeedeh Maleki, Amir Nasrolahi Shirazi, Mahboobeh Pordeli, Maliheh Safavi, Sussan Kabudanian Ardestani, Shaaban Asd, Rakesh Tiwari, Donghoon Oh, Abbas Shafiee, Alireza Foroumadi, Keykavous Parang, Tahmineh Akbarzadeh

Pharmacy Faculty Articles and Research

Several novel N-(9-oxo-9H-xanthen-4-yl)benzenesulfonamides derivatives were prepared as potential antiproliferative agents. The in vitro antiproliferative activity of the synthesized compounds was investigated against a panel of tumor cell lines including breast cancer cell lines (MDA-MB-231, T-47D) and neuroblastoma cell line (SK-N-MC) using MTT colorimetric assay. Etoposide, a well-known anticancer drug, was used as a positive standard drug. Among synthesized compounds, 4-methoxy-N-(9-oxo-9H-xanthen-4-yl)benzenesulfonamide (5i) showed the highest antiproliferative activity against MDA-MB-231, T-47D, and SK-N-MC cells. Furthermore, pentafluoro derivatives 5a and 6a exhibited higher antiproliferative activity than doxorubicin against human leukemia cell line (CCRF-CEM) and breast adenocarcinoma (MDAMB- 468) cells. Structure-activity relationship studies revealed …

Parent-Metabolite Pharmacokinetic Models For Tramadol – Tests Of Assumptions And Predictions, Sam Holford, Karel Allegaert, Brian J. Anderson, Butch Kukanich, Altamir B. Sousa, Amir Steinman, Bruno Pypendop, Reza Mehvar, Mario Giorgi, Nick Holford

Pharmacy Faculty Articles and Research

Allometric principles were used to discern cross-species differences in (±)-tramadol disposition and formation of its primary analgesic metabolite, (±)-O-desmethyl-tramadol (M1). Species differences in formation of M1 may help predict the analgesic effectiveness of tramadol. Tramadol was administered intravenously by a zero-order (constant infusion) process or rapid bolus dose and racemic concentrations of tramadol and M1 measured. Data were pooled to define differences between species (human, rat, cat, dog, goat, donkey and horse). A two-compartment linear disposition model with first-order elimination was used to describe tramadol and M1 disposition. Slow metabolizers were detected in 6% of the population and tramadol clearance …

Synthesis And Evaluation Of C-Src Kinase Inhibitory Activity Of Pyridin-2(1h)-One Derivatives, Karam Chand, Suchita Prasad, Rakesh Tiwari, Amir Nasrolahi Shirazi, Sumit Kumar, Keykavous Parang, Sunil K. Sharma

Pharmacy Faculty Articles and Research

Src kinase, a prototype member of the Src family of kinases (SFKs), is over-expressed in various human tumors, and has become a target for anticancer drug design. In this perspective, a series of eighteen 2-pyridone derivatives were synthesized and evaluated for their c-Src kinase inhibitory activity. Among them, eight compounds exhibited c-Src kinase inhibitory activity with IC50 value of less than 25 mu M. Compound 1-[2-(dimethylamino)ethyl]-5-(2-hydroxy-4-methoxybenzoyl)pyridin-2(1H)-one (36) exhibited the highest c-Src kinase inhibition with an IC50 value of 12.5 mu M. Furthermore, the kinase inhibitory activity of compound 36 was studied against EGFR, MAPK and PDK, however no significant activity …

Comparison Of Automated Event Detection Algorithms In Pathological Gait, Dustin A. Bruening, Sarah Trager Ridge

Faculty Publications

Accurate automated event detection is important in increasing the efficiency and utility of instrumented gait analysis. Published automated event detection algorithms, however, have had limited testing on pathological populations, particularly those where force measurements are not available or reliable. In this study we first postulated robust definitions of gait events that were subsequently used to compare kinematic based event detection algorithms across difficult pathologies. We hypothesized that algorithm accuracy would vary by gait pattern, and that accurate event detection could be accomplished by first visually classifying the gait pattern, and subsequently choosing the most appropriate algorithm. Nine published kinematic event …

2014 Weed Science Research Report – Blue Section, Ndsu Weed Science, Dept. Of Plant Sciences

Plant Sciences Weed Science Reports

The Weed Science team at North Dakota State University conduct field research in the State of North Dakota. This specific document reports on field research conducted in 2014. It includes work on corn, soybean and the management of several weeds in North Dakota.