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2014

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Articles 7321 - 7350 of 7603

Full-Text Articles in Life Sciences

In Vivo Embryo Production In Some Native Goat Breeds In Turkey, Ali̇ Reha Ağaoğlu, Kübra Karakaş, Mustafa Kaymaz Jan 2014

In Vivo Embryo Production In Some Native Goat Breeds In Turkey, Ali̇ Reha Ağaoğlu, Kübra Karakaş, Mustafa Kaymaz

Turkish Journal of Veterinary & Animal Sciences

The aim of this study was to assess the efficacy of the repeated administration of superovulatory protocol and surgical procedure in in vivo embryo production, as well as to determine the differences in the responses to superovulatory treatment among several Turkish native goat breeds (Angora, Kilis, Honamli, Hair, and Norduz). A superovulatory treatment procedure was repeated 3 times in this study. The estrus cycles were synchronized and superovulatory response was induced with porcine follicle-stimulating hormone during the natural breeding season. Natural mating was performed following CIDR removal. Embryo recovery was performed surgically on day 6, after the first mating. According …

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Inhibitory Effect Of K^+ And Ca^{2+} Concentrations, Ph, And Osmolality Of Activation Solution On Motility Of Shabut (Barbus Grypus Heckel 1843) Spermatozoa, Fati̇h Öğretmen, Selami̇ Gölbaşi, Burak Evren İnanan Jan 2014

Inhibitory Effect Of K^+ And Ca^{2+} Concentrations, Ph, And Osmolality Of Activation Solution On Motility Of Shabut (Barbus Grypus Heckel 1843) Spermatozoa, Fati̇h Öğretmen, Selami̇ Gölbaşi, Burak Evren İnanan

Turkish Journal of Veterinary & Animal Sciences

Evaluation of sperm quality is important in order to understand the main biochemical processes occurring during motility of sperm, to determine the reproductive ability of fish species, and to optimize the environment for spermatozoa. Generally, once differentiated in the gonad the sperm remain there completely quiescent until they are released into an external medium. After activation, motility duration of fish spermatozoa is greatly affected by environmental factors such as pH, ion concentrations, and osmotic pressure. Investigating these factors in relation to fish sperm can help establish good activation or immobilizing media for improving artificial fertilization and preservation. This study aimed …

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In Vitro Assessment On Intestinal Microflora From Commonly Farmed Fishes For Control Of The Fish Pathogen Edwardsiella Tarda, Jiun Yan Loh, Yau Yan Lim, Sharr Azni Harmin, Adeline Su Yien Ting Jan 2014

In Vitro Assessment On Intestinal Microflora From Commonly Farmed Fishes For Control Of The Fish Pathogen Edwardsiella Tarda, Jiun Yan Loh, Yau Yan Lim, Sharr Azni Harmin, Adeline Su Yien Ting

Turkish Journal of Veterinary & Animal Sciences

This study was aimed to evaluate the microflora isolated from the intestines of tilapia (Oreochromis niloticus), freshwater catfish (Clarias batrachus), snakehead (Ophicephalus striatus), snakeskin gourami (Trichogaster pectoralis), climbing perch (Anabas testudineus), and river catfish (Pangasius pangasius) for their potential probiotic development. Isolates were initially screened for their inhibitory effect against one of the most common fish pathogens, Edwardsiella tarda. Positive antagonists were subsequently tested for hemolytic activity, antibiotic susceptibility, and production of bacteriocin-like inhibitory substances (BLISs). Our results revealed 55 of the 405 isolates tested showed inhibitory effects towards the pathogen. Forty isolates were γ-hemolytic bacteria. Only 7 isolates were …

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The Effects Of Dietary Glutamine Supplementation On Growth Performance And Intestinal Morphology Of Broiler Chickens Reared Under Hot Conditions, Farhad Jazideh, Parviz Farhoomand, Mohsen Daneshyar, Gholamreza Najafi Jan 2014

The Effects Of Dietary Glutamine Supplementation On Growth Performance And Intestinal Morphology Of Broiler Chickens Reared Under Hot Conditions, Farhad Jazideh, Parviz Farhoomand, Mohsen Daneshyar, Gholamreza Najafi

Turkish Journal of Veterinary & Animal Sciences

Dietary supplementation effects of 0%, 0.25%, 0.5%, and 1% glutamine were investigated on the performance and development of the gastrointestinal tract in broilers reared under hot conditions. No significant differences were observed between the treatments for feed intake and feed conversion ratio during the starter period, grower period, or entire experiment (P > 0.05). During the grower period, only 0.5% glutamine-fed birds had higher body weight gain than others (P < 0.05). For the entire period, body weight gain of 0.5% glutamine-fed birds was greater than that of 0.00% and 0.25% glutamine-fed ones (P < 0.05). Furthermore, 0.5% glutamine-fed birds showed longer villus height than other birds (P < 0.05). In the jejunum, both 0.5% and 1% glutamine consumption caused longer villus height as compared to 0.00% and 0.25% glutamine (P < 0.05). Villus height of 0.25% glutamine-fed birds was higher than that of 0.00% glutamine-fed ones (P < 0.05). None of the ileum morphological parameters were affected by glutamine supplementation (P > 0.05). In conclusion, supplementation of 0.5% glutamine improved the intestinal morphology and body weight gain of broilers under heat stress. Higher dietary glutamine (1%) increased the villus height in the jejunum …

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The Effects Of Royal Jelly On Performance And Fatty Acid Profiles Of Different Tissues In Quail (Coturnix Coturnix Japonica) Reared Under High Stocking Density, İsmai̇l Seven, Ülkü Gülci̇han Şi̇mşek, Zehra Gökçe, Pinar Tatli Seven, Aslihan Arslan, Ökkeş Yilmaz Jan 2014

The Effects Of Royal Jelly On Performance And Fatty Acid Profiles Of Different Tissues In Quail (Coturnix Coturnix Japonica) Reared Under High Stocking Density, İsmai̇l Seven, Ülkü Gülci̇han Şi̇mşek, Zehra Gökçe, Pinar Tatli Seven, Aslihan Arslan, Ökkeş Yilmaz

Turkish Journal of Veterinary & Animal Sciences

To study the effects of royal jelly (RJ) on performance and fatty acid profiles, 168 unsexed 8-day-old quails (Coturnix coturnix japonica) were assigned to 4 treatment groups. Groups were arranged as follows: control (LSD; 160 cm2/quail and no supplementation), high stocking density (HSD; 80 cm2/quail and no supplementation), HSD-RJ 250 [80 cm2/quail and 250 mg/kg body weight (BW) RJ given orally], and HSD-RJ 500 (80 cm2/quail and 500 mg/kg BW RJ given orally). Body weight gain decreased significantly in the HSD group at day 42 (P < 0.01). Feed intake (FI) and feed conversion rate (FCR) were also significantly affected in the HSD group (P < 0.01). FI and FCR were improved by both doses of RJ. Both RJ doses increased total polyunsaturated fatty acid ratios in the breast, kidney (P < 0.001), leg, and liver (P < 0.05). Total saturated fatty acid ratios in leg (P < 0.01) and kidney (P < 0.05) tissues increased in the HSD group but decreased in the livers of HSD-RJ 250 group (P < 0.05). In conclusion, RJ supplementation improved performance parameters and unsaturated fatty acid ratios in the examined quail tissues.

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Serological Survey Of Equid Herpesvirus 3 Infection In Turkish Horses, Veysel Soydal Ataseven, Firat Doğan, Seval Bi̇lge Dağalp, Mehmet Tolga Tan, Köksal Altinbay, Cafer Tayyar Ateş Jan 2014

Serological Survey Of Equid Herpesvirus 3 Infection In Turkish Horses, Veysel Soydal Ataseven, Firat Doğan, Seval Bi̇lge Dağalp, Mehmet Tolga Tan, Köksal Altinbay, Cafer Tayyar Ateş

Turkish Journal of Veterinary & Animal Sciences

Sera from 1133 Turkish horses were obtained and tested for equid herpesvirus 3 (EHV-3) antibodies using a sero-neutralisation test. The sample consisted of 420 privately owned working horses from 2 regions of Turkey, 428 brood horses from private stud farms and breeding stations, and 285 racehorses from private stud farms. Neutralising antibodies against EHV-3 were found in 287 (25.3%) of the 1133 analysed sera. The highest seroprevalence for EHV-3 was detected in the brood horses (51.2%), followed by the racehorses (10.2%), and the working horses (9.3%). The seroprevalence rates tended to be higher in female horses than in male horses …

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Immunohistochemical Distribution Of Glucagon -, Insulin -, Somatostatin -, Gastrin-,And Serotonin-Containing Cells In The Pancreas Of The Van Cat, Turan Karaca, Adem Kara, Nejdet Şi̇mşek, Sema Uslu, Deni̇z Teki̇ner, Meci̇t Yörük Jan 2014

Immunohistochemical Distribution Of Glucagon -, Insulin -, Somatostatin -, Gastrin-,And Serotonin-Containing Cells In The Pancreas Of The Van Cat, Turan Karaca, Adem Kara, Nejdet Şi̇mşek, Sema Uslu, Deni̇z Teki̇ner, Meci̇t Yörük

Turkish Journal of Veterinary & Animal Sciences

The regional distribution, relative frequency, and appearance of glucagon (A-cell)-, insulin (B-cell)-, somatostatin (D-cell)-, gastrin (G-cell)-, and serotonin (EC-cell)-secreting cells in the endocrine and exocrine pancreas of Van cats were examined using the immunohistochemistry method. Glucagon immunopositive A-cells were principally found in the central region of the islets of Langerhans, while insulin immunopositive B-cells were located in the periphery of the pancreatic islets. Moreover, several A- and B-cells were observed as only single cells or clusters of 2 to 3 immunopositive cells in the exocrine parenchyma and the pancreatic duct epithelium. Somatostatin and gastrin immunopositive reactivities were negligible in the …

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Milk Fatty Acid Composition From Goats In A Semiintensive Production System In An Arid Region Of The Peninsula Of Baja California, Mexico, Eduardo Alberto Toyes Vargas, Hector Gonzalez Garcia, Miguel Victor Cordoba Matson, Ricardo Ortega Perez, Jose Luis Espinoza Villavicencio, Alejandro Palacios Espinosa, Narciso Ysac Avila Serrano, Bernardo Murillo Amador Jan 2014

Milk Fatty Acid Composition From Goats In A Semiintensive Production System In An Arid Region Of The Peninsula Of Baja California, Mexico, Eduardo Alberto Toyes Vargas, Hector Gonzalez Garcia, Miguel Victor Cordoba Matson, Ricardo Ortega Perez, Jose Luis Espinoza Villavicencio, Alejandro Palacios Espinosa, Narciso Ysac Avila Serrano, Bernardo Murillo Amador

Turkish Journal of Veterinary & Animal Sciences

The aim of this research was to study the effects of seasons (rainy and dry) on the composition of the fatty acids (FAs) in milk of Creole goats in a semiintensive production system. Milk sampling was carried out in the dry season (June) and just after the rainy season (November) in an arid region of Baja California Sur, Mexico. The goat diet was composed of native species it grazed on in the rangeland, as well as hayed forages with some supplements containing approximately equal amounts of sorghum and maize. The results for different types of FAs in goat milk showed …

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Nmr-Based Metabolomic Evaluation In Dairy Cows With Displaced Abomasum, Abdullah Başoğlu, Nuri̇ Başpinar, Alparslan Coşkun Jan 2014

Nmr-Based Metabolomic Evaluation In Dairy Cows With Displaced Abomasum, Abdullah Başoğlu, Nuri̇ Başpinar, Alparslan Coşkun

Turkish Journal of Veterinary & Animal Sciences

This study is the first to evaluate nuclear magnetic resonance (NMR)-based metabolomics in cows with displaced abomasum (DA), which is an internationally recognized problem in dairy cows. Some biochemical parameters have been used for monitoring DA. However, to date, few data have been available on the blood metabolomic profile of dairy cows. Forty Holstein multiparous cows with DA (30 left, 10 right) and 10 clinically healthy Holstein multiparous cows were the subjects of the study. All the animals had similar dry matter intake. An NMR-based metabolomics approach and hematological and biochemical analyses were performed. Some changes in biochemical parameters were …

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A Disseminated Cutaneous Plasmacytoma Case In A Dog, Burçak Özkan, Si̇nem Ülgen, Funda Yildirim, Ali̇ Riza Kiziler, Kürşat Özer, Mehmet Erman Or, Aydin Gürel, Ümi̇t Bora Barutçu, Hazi̇m Tamer Dodurka Jan 2014

A Disseminated Cutaneous Plasmacytoma Case In A Dog, Burçak Özkan, Si̇nem Ülgen, Funda Yildirim, Ali̇ Riza Kiziler, Kürşat Özer, Mehmet Erman Or, Aydin Gürel, Ümi̇t Bora Barutçu, Hazi̇m Tamer Dodurka

Turkish Journal of Veterinary & Animal Sciences

A 5-year-old mixed-breed dog presenting with diffuse lesions was diagnosed with cutaneous plasmacytoma. Sites where tumors occurred most often involved the skin of the facial areas, as well as that of the shoulder, neck, and back of the body. No systemic findings were present in spite of a significant enlargement of the lesions. The tumors varied in diameter, the largest being 5-10 cm. Tissue samples obtained from tumoral masses were fixed in 10% formalin, processed routinely, embedded in paraffin, cut into sections of 4-5 µm, and stained with hematoxylin and eosin. To determine the nature of the tumoral cells, kappa …

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Severe Beak Deformity In Melopsittacus Undulatus Caused By Knemidocoptes Pilae, Pedro María Alarcón Elbal, Victor Jesus Carmona Salido, José Marín Sánchez Murillo, Rafael Calero Bernal, Javier Lucientes Curdi Jan 2014

Severe Beak Deformity In Melopsittacus Undulatus Caused By Knemidocoptes Pilae, Pedro María Alarcón Elbal, Victor Jesus Carmona Salido, José Marín Sánchez Murillo, Rafael Calero Bernal, Javier Lucientes Curdi

Turkish Journal of Veterinary & Animal Sciences

An outbreak of knemidocoptic mange caused by Knemidocoptes pilae is described in caged Australian budgerigars (Melopsittacus undulatus) in a public park in L´Eliana (Valencia, Spain). Five individuals (5 out of 70) displayed severe malformation of the beak, with the rhamphotheca, rhinotheca, and gnathotheca affected and a slight lateral deviation of the jawbone, which did not, however, impede feeding or grooming.

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Expression Of The Gprc5d Gene In Liaoning Cashmere Goats, Mei Jin, Xiuna Li, Jun Piao, Yangle Qu, Yimeng Wang, Jingai Piao, Mingxing Chu Jan 2014

Expression Of The Gprc5d Gene In Liaoning Cashmere Goats, Mei Jin, Xiuna Li, Jun Piao, Yangle Qu, Yimeng Wang, Jingai Piao, Mingxing Chu

Turkish Journal of Veterinary & Animal Sciences

This study aimed to characterize the expression the GPRC5D gene in the skin of the Liaoning Cashmere goat. Initially, the GPRC5D gene sequence was subjected to bioinformatics analysis. Second, semiquantitative RT-PCR was used to detect GPRC5D gene expression in the tissues. Third, in situ hybridization was used to locate GPRC5D gene expression in the primary and secondary hair follicles. Functional site analysis showed that GPRC5D has 7 transmembrane domains. RT-PCR showed that GPRC5D was expressed only in the skin. In situ hybridization showed that GPRC5D was expressed in the inner root sheath only. We speculated that GPRC5D may regulate cashmere …

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A Detailed Evaluation Of The Skeletal Elements Of The Skull In The Grey Heron (Ardea Cinerea), Şükrü Hakan Atalgin, Emi̇ne Ümran Bozkurt Büyükçopur, İbrahi̇m Kürtül Jan 2014

A Detailed Evaluation Of The Skeletal Elements Of The Skull In The Grey Heron (Ardea Cinerea), Şükrü Hakan Atalgin, Emi̇ne Ümran Bozkurt Büyükçopur, İbrahi̇m Kürtül

Turkish Journal of Veterinary & Animal Sciences

This study was designed to reveal, in detail, the features of the skull bones in the grey heron, a species of the family Ardeidae, and to compare the findings with previous related literature reports and with Nomina Anatomica Avium. The articulation between the nasal and frontal bones was observed to be through the movable frontonasal joint. The upper beak fused with the neurocranium via this joint and with the palatine and vomer. It was also bound indirectly through the zygomatic bone articulating with the quadrate bone. The frontal bone possessed the frontal depression dorsally. Wideness of the frontal bone and …

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Immunohistochemical Localization Of Transforming Growth Factor Beta 2 And Gene Expression Using Real-Time Pcr In Capsaicin-Administered Rat Testis During Puberty, Gökhan Nur, Mümtaz Nazli, Sevda Eli̇ş Yildiz Jan 2014

Immunohistochemical Localization Of Transforming Growth Factor Beta 2 And Gene Expression Using Real-Time Pcr In Capsaicin-Administered Rat Testis During Puberty, Gökhan Nur, Mümtaz Nazli, Sevda Eli̇ş Yildiz

Turkish Journal of Veterinary & Animal Sciences

The objective of this study was to investigate the effects of capsaicin on the testis distribution of transforming growth factor beta 2 (TGF\beta2) and gene expression using real-time PCR in pubescent rats. In this study, rats were divided into 3 groups: capsaicin (CAP), sham, and control. The CAP group was injected with 1 mg/kg doses of capsaicin (10% ethanol and 1% Tween in an 80% distilled water mixture) subcutaneously every day for a period of 1 week. The sham group was injected with a solution of 10% ethanol and 1% Tween in a mixture of 80% distilled water and nothing …

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Pathotypic Characterization Of The Newcastle Disease Virus Isolated From Commercial Poultry In Northwest Iran, Elham Ahmadi, Seyed Ali Pourbakhsh, Malahat Ahmadi, Alireza Talebi Jan 2014

Pathotypic Characterization Of The Newcastle Disease Virus Isolated From Commercial Poultry In Northwest Iran, Elham Ahmadi, Seyed Ali Pourbakhsh, Malahat Ahmadi, Alireza Talebi

Turkish Journal of Veterinary & Animal Sciences

Despite vaccination, outbreaks of Newcastle disease in commercial poultry flocks in northwest Iran have been regularly reported in recent years. This research was proposed in order to characterize, through a molecular approach, the genetic nature of strains of Newcastle disease virus isolated from commercial poultry flocks in northwest Iran. Ten Newcastle disease virus isolates were obtained from various districts of northwest Iran in 2010. The isolates were screened with F gene-targeting reverse transcription polymerase chain reaction (RT-PCR). The amplified products of virulent strains were sequenced. The deduced amino acid sequence of the fusion protein cleavage site indicated the presence of …

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Evaluation Of Ultrasonography As A Diagnostic Tool For Hepatic Hydatid Cysts In Sheep, Hussein Awad Hussein, Mohammed Elrashidy Jan 2014

Evaluation Of Ultrasonography As A Diagnostic Tool For Hepatic Hydatid Cysts In Sheep, Hussein Awad Hussein, Mohammed Elrashidy

Turkish Journal of Veterinary & Animal Sciences

This retrospective study was designed to evaluate the use of ultrasonography for the diagnosis of hepatic hydatid cysts. This study was conducted on 22 sheep, which were classified into 2 groups according to their liver health group (healthy liver = 13 and cystic liver = 9). Biochemically, serum concentrations of gamma-glutamyl transferase, aspartate aminotransferase, total bilirubin, and globulins were significantly increased (P < 0.01) while albumin was lowered (P < 0.01) in sheep with cystic livers. Ultrasonographically, rounded, anechoic, and unilocular hydatid cysts with elliptical circumferences ranging from 6 to 10 cm were seen. The interiors of cysts contained echogenic particulate materials, septations, or fine echoes. At the 10th intercostal space (ICS), ventral margin, size, thickness, and angle of livers increased in sheep with hepatic cysts, while the diameter of portal vein decreased in sheep with liver cysts. At the 9th ICS, the circumference of the gall bladder was lowered in sheep with hepatic cysts (P < 0.01). The sensitivity, specificity, and positive and negative predictive values of ultrasonography for diagnosis of hepatic hydatid cysts were 80%, 100%, and 100% and 83%, respectively. In conclusion, ultrasonography alone or in combination with some biochemical parameters reflecting liver function could be helpful for the diagnosis of hepatic hydatid cysts in sheep.

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The Effects Of Lh On Progesterone Production By Cell Subpopulations Isolated From Early And Late Luteal Phase Goat Corpora Lutea, Hakan Kalender, Şevket Arikan, Özkan Şi̇mşek Jan 2014

The Effects Of Lh On Progesterone Production By Cell Subpopulations Isolated From Early And Late Luteal Phase Goat Corpora Lutea, Hakan Kalender, Şevket Arikan, Özkan Şi̇mşek

Turkish Journal of Veterinary & Animal Sciences

The objective of this study was to investigate the complementary effects of luteinizing hormone (LH) on cell subpopulations. Corpora lutea were collected on days 5 and 15 of the cycle. The luteal tissues were treated with collagenase for cell dissociation. Percoll density-gradient centrifugation was performed to separate cells into 2 distinct subpopulations. Luteal cells (5 × 104 cells/well) were cultured with and without 22(R)-hydroxycholesterol (22R-HC, 10 µg/mL) and LH (10 and 100 ng/mL) alone, and also with 22R-HC (10 µg/mL) plus LH (10 and 100 ng/mL), in a serum-free culture medium for 6 days of incubation. Untreated cells isolated from …

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Determination Of Plasma Fibrinogen And Haptoglobin, Hematological And Blood Biochemical Changes In Bulgarian Local Goats With Experimentally Induced Staphylococcus Aureus Mastitis, Ivan Fasulkov, Manol Karadaev, Nasko Vasilev, Valentina Urumova, Teodora Mircheva Jan 2014

Determination Of Plasma Fibrinogen And Haptoglobin, Hematological And Blood Biochemical Changes In Bulgarian Local Goats With Experimentally Induced Staphylococcus Aureus Mastitis, Ivan Fasulkov, Manol Karadaev, Nasko Vasilev, Valentina Urumova, Teodora Mircheva

Turkish Journal of Veterinary & Animal Sciences

The aim of the present study was to assess changes in acute phase proteins fibrinogen and haptoglobin, as well as hematological, blood biochemical, and microbiological alterations in local Bulgarian goats with experimentally induced Staphylococcus aureus mastitis. The experiments were conducted with 6 clinically healthy local Bulgarian goats, 2 to 6 years of age, weighing 46-57 kg. The experimental infection was done with a stationary phase culture of a field Staphylococcus aureus strain isolated from a cow with clinical mastitis. Blood haptoglobin in goats was considerably higher (P < 0.05) as early as 8 h after the pathogen's inoculation. The most significant differences from baseline values occurred 24 and 48 h (P < 0.01) after infection. Plasma fibrinogen increased significantly (P < 0.05) 8 h after infection and reached the highest mean concentration (9.12 g/L) by 72 h. Leukocytosis was established as early as 4 h after the experimental infection when leukocyte counts were significantly higher than baseline (P < 0.001). The analysis of results indicates that in experimentally induced Staphylococcus aureus mastitis, significantly higher (P < 0.05) levels of the leukocyte, fibrinogen, and haptoglobin are established as early as 8 h after the infection.

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Proximate Composition And Mineral And Fatty Acid Profiles Of Male And Female Jinga Shrimps (Metapenaeus Affinis, H. Milne Edwards, 1837), Mehmet Tolga Di̇nçer, İlker Aydin Jan 2014

Proximate Composition And Mineral And Fatty Acid Profiles Of Male And Female Jinga Shrimps (Metapenaeus Affinis, H. Milne Edwards, 1837), Mehmet Tolga Di̇nçer, İlker Aydin

Turkish Journal of Veterinary & Animal Sciences

The jinga shrimp (Metapenaeus affinis, H. Milne Edwards, 1837) is a commercially valuable alien decapod and a seafood product that is highly popular at seafood restaurants in İzmir (Turkey). The chemical, mineral, and fatty acid compositions of male and female jinga shrimps harvested in the Mediterranean Sea were determined for the first time. The compositions of both sexes confirmed that the jinga shrimp is a healthy food source due to its balance of nutrients, with efficient levels of protein and mineral contents. In fatty acid composition, the saturated fatty acid fraction was dominant, followed by polyunsaturated fatty acid and monounsaturated …

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Screening For S32g Mutation Of Bmp15 Gene In 18 Goat Breeds, Tao Feng, Ming-Xing Chu, Gui-Ling Cao, Dong-Wei Huang, Ran Di, Qiu-Yue Liu, Zhang-Yuan Pan, Mei Jin, Ying-Jie Zhang Jan 2014

Screening For S32g Mutation Of Bmp15 Gene In 18 Goat Breeds, Tao Feng, Ming-Xing Chu, Gui-Ling Cao, Dong-Wei Huang, Ran Di, Qiu-Yue Liu, Zhang-Yuan Pan, Mei Jin, Ying-Jie Zhang

Turkish Journal of Veterinary & Animal Sciences

In our early work, one mutation (A898G, which caused Ser32Gly (S32G) change of mature peptide) was identified in the goat BMP15 gene and the allele G was associated with high litter size in Jining Grey goats (the goat breed with the highest fecundity in China). The aim of this research was to investigate the genetic structure of the prolific BMP15 gene in 18 goat breeds reared in China, including 12 native breeds, 3 introduced breeds, and 2 cultivated breeds. Genomic DNA of 1011 goats was screened for the S32G mutation. The results showed that this mutation existed in all 3 …

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Polymorphism In Introns 5 And 6 Of The Acta1 Gene In Various Polish Horse Breeds, Daniel Polasik, Ryszard Pikula Jan 2014

Polymorphism In Introns 5 And 6 Of The Acta1 Gene In Various Polish Horse Breeds, Daniel Polasik, Ryszard Pikula

Turkish Journal of Veterinary & Animal Sciences

The aim of this study was to find polymorphisms in the actin alpha 1 gene (ACTA1) in horses belonging to different breeds and utility types and to compare them. The genomic region of the actin alpha 1 gene was positively selected in purebred horses. By sequencing part of the ACTA1 gene, 2 polymorphic sites located in intron 5 (G > T) and intron 6 (G > C) were found. The polymerase chain reaction-restriction fragment length polymorphism and the amplification created restriction site-polymerase chain reaction methods were applied to screen these polymorphisms. An analysis of the genotype and allele frequencies showed differences depending …

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Repeated Superovulation Treatments In Kivircik Ewes During Breeding And Nonbreeding Seasons, Burcu Üstüner, Seli̇m Alçay, Yavuz Nak, Zekari̇ya Nur, Deni̇z Nak, Bi̇lgi̇ner Tuna, Gözde Şi̇mşek, Hakan Sağirkaya Jan 2014

Repeated Superovulation Treatments In Kivircik Ewes During Breeding And Nonbreeding Seasons, Burcu Üstüner, Seli̇m Alçay, Yavuz Nak, Zekari̇ya Nur, Deni̇z Nak, Bi̇lgi̇ner Tuna, Gözde Şi̇mşek, Hakan Sağirkaya

Turkish Journal of Veterinary & Animal Sciences

The present study compared superovulation and embryo recovery rates applied repeatedly during breeding and nonbreeding seasons in the same 20 Kivircik sheep. Trials 1 and 3 were performed during the breeding season and the trial 2 was performed in the nonbreeding season. We synchronized the sheep's estrus cycles and induced a superovulatory response with porcine follicle stimulating hormone (pFSH) injections. Natural mating took place 12 h after the last FSH injection. The embryos were collected by laparotomy 7 days after natural mating. There was no significant difference between the number of corpora lutea (CL) counted following the 1st (breeding) and …

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Impact Of The Depletion Of High-Abundance Proteins In Blood Plasma/Serum On The Proteome Of These Media In Growing Farm Animals, Adam Lepczynski, Alicja Dratwa-Chalupnik, Agnieszka Herosimczyk, Katarzyna Staszak, Arleta Majewska, Malgorzata Ozgo Jan 2014

Impact Of The Depletion Of High-Abundance Proteins In Blood Plasma/Serum On The Proteome Of These Media In Growing Farm Animals, Adam Lepczynski, Alicja Dratwa-Chalupnik, Agnieszka Herosimczyk, Katarzyna Staszak, Arleta Majewska, Malgorzata Ozgo

Turkish Journal of Veterinary & Animal Sciences

The aim of the study was to use hexapeptide combinatorial libraries to evaluate the influence of high-abundance protein depletion on low-abundance protein enrichment, protein concentration, and 2D gel quality in plasma and serum proteomes of growing piglets and calves. Depleted and nondepleted samples of plasma/serum were resolved using 2D electrophoresis (2DE). Bioinformatic analysis of the 2D gels showed an increased number of detected spots on each gel after depletion of excess high-abundance proteins in calf plasma (+26.46%) and in piglet serum (+49.40%). Relative expression of selected low-abundance protein spots in the blood plasma/serum samples was higher after high-abundance protein depletion. …

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Herd-Specific Autovaccine And Antibiotic Treatment In Elimination Of Staphylococcus Aureus Mastitis In Dairy Cattle, Danuta Czernomysy-Furowicz, Karol Fijalkowski, Anna Silecka, Jolanta Karakulska, Pawel Nawrotek, Radoslaw Drozd, Magdalena Ferlas, Jacek Borkowski, Dorota Jankowiak Jan 2014

Herd-Specific Autovaccine And Antibiotic Treatment In Elimination Of Staphylococcus Aureus Mastitis In Dairy Cattle, Danuta Czernomysy-Furowicz, Karol Fijalkowski, Anna Silecka, Jolanta Karakulska, Pawel Nawrotek, Radoslaw Drozd, Magdalena Ferlas, Jacek Borkowski, Dorota Jankowiak

Turkish Journal of Veterinary & Animal Sciences

The aim of the present study was to compare the effectiveness of cefuroxime, herd-specific autovaccine, and cefuroxime/herd-specific autovaccine treatments in eliminating S. aureus from the milk of 45 cows with subclinical mastitis. Prior to this study, cows with S. aureus mastitis were divided into 3 groups of 15 cows each. Group 1 received cefuroxime, group 2 received the autovaccine, and group 3 received cefuroxime and the autovaccine. The antibiotic was infused into the teat canal, whereas the autovaccine was administrated in the upper udder lymph node region. It was revealed that after day 35 of the treatment S. aureus was …

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Detection Of Sensitive And Mutant Ruminal Bacteria Isolates From Sheep, Cattle, And Buffalo Using 14 Therapeutic Antibiotics, Abdelfattah Zeidan Mohamed Salem, Roberto Montes De Oca Jimenez, Maria Andrea Cerrillo-Soto, Ahmed Eid Kholif, Mohamed Zidan Mohamed Salem, Mona Mohamed Mohamed Yasseen Elghandour Jan 2014

Detection Of Sensitive And Mutant Ruminal Bacteria Isolates From Sheep, Cattle, And Buffalo Using 14 Therapeutic Antibiotics, Abdelfattah Zeidan Mohamed Salem, Roberto Montes De Oca Jimenez, Maria Andrea Cerrillo-Soto, Ahmed Eid Kholif, Mohamed Zidan Mohamed Salem, Mona Mohamed Mohamed Yasseen Elghandour

Turkish Journal of Veterinary & Animal Sciences

In the present study, sensitive and mutant colonies of some ruminal bacterial species isolated from sheep, cattle, and buffalo were detected. We counted and considered \"mutant colonies\" the bacterial colonies grown in the clear inhibition zone in the Kirby-Bauer disk diffusion susceptibility test. Detected mutant colonies were higher in buffalo than in cattle and sheep. Duricef and metronidazole caused no mutations in any species. The others formed mutant colonies, where roxithromycin = polymyxin = chloramphenicol = gentamicin < erythromycin < vancomycin < piperacillin = cefotaxime < streptomycin < cefoperazone < ciprofloxacin < amikacin. Sheep had the highest number of sensitive isolates, and the number of sensitive isolates was dramatically lower in cattle and buffalo. There were no sensitive isolates with the antibiotic metronidazole, and there was a low number of sensitive isolates with duricef. The other antibiotics had more sensitive isolates (gentamicin = ciprofloxacin = amikacin > streptomycin = piperacillin > erythromycin > vancomycin = cefoperazone = cefotaxime > roxithromycin > polymyxin > chloramphenicol). The number of sensitive isolates of the different ruminant species for …

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Effects Of Supplementation Of Inorganic Sulfur On Some Biochemical Parameters In Angora Goat’S Diet Containing High Nitrate Levels, Mehmet Özdemi̇r, Abdullah Eryavuz, Gülcan Avci, Yavuz Osman Bi̇rdane, İsmai̇l Küçükkurt Jan 2014

Effects Of Supplementation Of Inorganic Sulfur On Some Biochemical Parameters In Angora Goat’S Diet Containing High Nitrate Levels, Mehmet Özdemi̇r, Abdullah Eryavuz, Gülcan Avci, Yavuz Osman Bi̇rdane, İsmai̇l Küçükkurt

Turkish Journal of Veterinary & Animal Sciences

The purpose of this study was to determine the effect of supplementation of sulfur to a diet containing sodium nitrate at a level that would cause chronic nitrate poisoning on some rumen and biochemical parameters in Angora goats. A total of 18 male Angora goats were used. The animals in the control group were fed the basal diet, while the animals were fed experimental concentrate containing 1500 mg/kg sodium nitrate in the nitrate group (N) and experimental concentrate containing 1500 mg/kg sodium nitrate plus 1.8% sodium sulfate in the nitrate-sulfur group (NS) throughout the study, which lasted for 6 months. …

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Recent Advances In The Immunology And Uterine Microbiology Of Healthy Cows And Cows That Develop Uterine Disease, Klibs N. Galvao, Jose Eduardo P. Santos Jan 2014

Recent Advances In The Immunology And Uterine Microbiology Of Healthy Cows And Cows That Develop Uterine Disease, Klibs N. Galvao, Jose Eduardo P. Santos

Turkish Journal of Veterinary & Animal Sciences

Uterine diseases are highly prevalent in dairy cows. Causes of uterine diseases are multifactorial. There is good evidence for the susceptibility of the host and for the role of pathogenic bacteria, and less evidence for the effect of the environment. Uterine and leukocyte immune response is impaired early postpartum in cows that develop uterine disease. The decrease in immune function is associated with a decrease in calcium postpartum and an increase in NEFA and BHBA. Both endometrial cells and granulosal cells possess toll-like receptors (TLR) that can recognize and mount an inflammatory response to pathogen-associated molecular patterns (PAMPs) such as …

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The Micrornas Important For Ovarian And Early Embryonic Development In Cattle, Lei Wang, Jianbo Yao Jan 2014

The Micrornas Important For Ovarian And Early Embryonic Development In Cattle, Lei Wang, Jianbo Yao

Turkish Journal of Veterinary & Animal Sciences

Recent progress in genomic analysis and other biochemical methods has led to the discovery of a large population of microRNAs (miRNAs), which have been demonstrated to play important roles in diseases and a wide range of developmental processes. Characterization of miRNA expression profiles in different stages of ovarian follicular development and early embryogenesis has suggested the potential roles of miRNAs in follicular development, maturation of oocytes, and preimplantation embryonic development. This review focuses on the current studies of miRNAs involved in ovarian and early embryonic development in cattle.

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Usefulness Of Short Sequence Repeat Markers In Goat Genetic Diversity Studies On The Asian And African Continents, Faiz M.M.T. Marikar, Muneeb M. Mustafa Jan 2014

Usefulness Of Short Sequence Repeat Markers In Goat Genetic Diversity Studies On The Asian And African Continents, Faiz M.M.T. Marikar, Muneeb M. Mustafa

Turkish Journal of Veterinary & Animal Sciences

Goat genetic diversity studies are very important since extinction of germ plasma is increasing very rapidly. The African and Asian continents are the hotspots for indigenous animals with fewer genetic manipulations. Conservation studies using molecular markers have the capability to validate the real status of the animals. The use of molecular markers has revolutionized studies of genetic diversity. Even though a number of markers are used for these types of studies, short sequence repeats are in the forefront due to their superior features such as high variability, high mutation rate, large number, distribution throughout the genome, codominant inheritance, and neutrality …

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New Understanding Of An Old Phenomenon: Uncontrolled Factors And Misconceptions That Cast A Shadow Over Studies Of The ‘Male Effect’ On Reproduction In Small Ruminants, Trina Jorre De St Jorre, Penny Alison Rhian Hawken, Graeme Bruce Martin Jan 2014

New Understanding Of An Old Phenomenon: Uncontrolled Factors And Misconceptions That Cast A Shadow Over Studies Of The ‘Male Effect’ On Reproduction In Small Ruminants, Trina Jorre De St Jorre, Penny Alison Rhian Hawken, Graeme Bruce Martin

Turkish Journal of Veterinary & Animal Sciences

In female sheep and goats, exposure to males can be used to induce ovulation and thus control and synchronise fertility. This 'male effect' offers simple, cost-effective, and hormone-free management of reproduction in flocks and herds but, despite decades of research, significant gaps in our knowledge impede commercial application. Many of these gaps can be traced back to misconceptions in the early documentation of the male effect, and others are the result of uncontrolled factors in experimental design. Consequently, it is often difficult to draw conclusions from past research, to further our understanding of the phenomenon, and to make recommendations for …

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