Life Sciences Commons^™

Use Of Remote Imagery And Object-Based Image Methods To Count Plants In An Open-Field Container Nursery, Josue Nahun Leiva

Graduate Theses and Dissertations

In general, the nursery industry lacks an automated inventory control system. Object-based image analysis (OBIA) software and aerial images could be used to count plants in nurseries. The objectives of this research were: 1) to evaluate the effect of an unmanned aerial vehicle (UAV) flight altitude and plant canopy separation of container-grown plants on count accuracy using aerial images and 2) to evaluate the effect of plant canopy shape, presence of flowers, and plant status (living and dead) on counting accuracy of container-grown plants using remote sensing images. Images were analyzed using Feature Analyst® (FA) and an algorithm trained using …

Spatial Distribution And Genetic Variability Of Reniform And Lance Nematodes In Cotton Agroecosystems, Claudia Holguin

All Dissertations

Rotylenchulus reniformis (reniform nematode) and Hoplolaimus columbus (Columbia lance nematode) are two of the main plant-pathogenic nematode species affecting cotton production in the United States. For the selection and implementation of effective management practices it is important to understand distribution patterns and population genetic structure of these two nematodes in cotton agroecosystems. In a three-year field study, the spatial distribution of reniform nematode was characterized in two commercial fields in South Carolina to determine the effects of relations with soil texture and crop rotation on reniform nematode distribution (Chapter 1). Results of this project suggest that population densities of reniform …

Effects Of Transportation Hazards On Package Performance And Food Product Shelf Life, Kyle Dunno

All Dissertations

This research studied the effect of transportation hazards on food product shelf life and package performance. Studies were conducted to determine the effect of package headspace volume, product viscosity and storage temperature on package integrity. Finally, accelerated shelf life testing (ASLT) was utilized to determine how simulated transportation hazards affected the shelf life of a specific food product. Institutional retort pouches containing either water or 5% starch solution were filled with varying amounts of headspace volume to determine if package headspace volume could aid in package performance during simulated engineering tests for packaged products. Fixed displacement vibration testing and compression …

Allosteric Regulation Of Bacterial And Fungal Xylulose 5-Phosphate/ Fructose 6-Phosphate Phosphoketolases (Xfps), Katie Glenn

All Dissertations

Acetate is excreted as a metabolic end product in many microbes. Acetate production has primarily been studied in bacteria and archaea but is known to occur in eukaryotic organisms as well. For example, acetate is one of the most abundant metabolites excreted by the fungal pathogen Cryptococcus neoformans during cryptococcosis suggesting that acetate production may be important during pathogenesis. One possible pathway for acetate production in C. neoformans involves the enzymes xylulose 5-phosphate/ fructose 6-phosphate phosphoketolase (Xfp), which can generate acetyl phosphate from either fructose 6-phosphate (F6P) or xylulose 5-phosphate (X5P), and acetate kinase (Ack), which can then convert acetyl …

Biology And Control Of The Bed Bug Cimex Lectularius L., Kevin Hinson

All Dissertations

After vanishing from the public eye for more than 50 years, bed bugs have resurged to become one of the most widely discussed and heavily researched insect pests in the westernized world. Our inability to prevent and successfully treat infestations has been the driving force behind this wave of research. I addressed gaps in our understanding of bed bugs by examining behavioral and life history characteristics, as well as insecticide application responses. I showed that natural-based products are generally ineffective against bed bugs, particularly when used as a residual treatment. I also found that bed bugs may be killed through …

Phylogenomics And Historical Biogeography Of The Gooseneck Barnacle Pollicipes Elegans, Sergio Andres Marchant Rojas

All Dissertations

This dissertation explores the systematics, biogeography, and genomics of the gooseneck barnacle Pollicipes elegans, a marine crustacean of the tropical Eastern Pacific. In Chapter 1, I provide a broad framework for my research by introducing and focusing on the long-­‐standing debate of the mechanisms behind the latitudinal gradient in species diversity, which provided the initial motivation for using Pollicipes elegans as a model system to study the mechanisms leading to genetic differentiation and speciation in tropical regions. In Chapter 2, I examine the genetic structure, infer patterns of connectivity across the warm tropical waters of the eastern Pacific, and reconstruct …

Genetic Dissection Of Neuromuscular Diseases Affecting Domestic Dogs, Caitlin Rinz

All Dissertations

The domestic dog, Canis familiaris, has a unique population structure that lends itself to the study of hereditary diseases. Purebred dogs populations are genetically isolated and as a result are affected by more than 400 naturally occurring diseases, many of which have human counterparts. In the last 15 years, dogs have emerged as a model for the study of human hereditary diseases, fueling the development of resources including a 7.6X coverage reference genome and single nucleotide polymorphism (SNP) genotyping arrays.

Congenital myasthenic syndrome (CMS) is a neuromuscular disorder of both humans and dogs in which transmission across the neuromuscular …

Effects Of Step Training On The Kidneys Following Spinal Cord Injury In Rats., Pradeepa Poudyal

Electronic Theses and Dissertations

Detrusor-sphincter dyssynergia after chronic spinal cord injury (SCI) leads to over-distention, high bladder pressures and vesico-ureteric reflux into the kidney, resulting in repeated infections and pyelonephritis. Given the importance/limited knowledge of SCI effects on the upper urinary tract, this study assessed expression of two proteins in the kidneys of contused (T9) male rats relative to controls that are indicative of tissue stress/damage (TGFβ, CD11b). The impact of two therapeutic rehabilitation strategies, 60 minutes of step training and general exercise, was then assessed on these protein levels relative to non-trained SCI controls, plus the effectiveness of a shorter training duration (30 …

The Effect Of A Controlled Frequency Breath Holding Training Program On Running Economy Among Elite College Swimmers., Patrick Sims 1988-

Electronic Theses and Dissertations

Running economy (RE) is the amount of oxygen consumed while running at a submaximal intensity. Along with aerobic capacity (VO2max), RE is an important predictor of running performance. Little research has investigated changes in RE with restricted breathing training [i.e. controlled breath-holding (CFB)] during exercise. RE may improve ~6% amongst a novice swimming cohort through CFB training, but this has not been established in elite swimmers. The purpose was to further establish that CFB training (16 sessions of 12 x 50-m with ~15 seconds rest between each 50-m, using only ~2 breaths per 50-m) can improve RE in 25 elite …

Cross-Cultural Comparison In The Emotional Responses Elicited By Wine Odors, Aimee Hasenbeck

Graduate Theses and Dissertations

To remain competitive in the wine market, wine companies must strive for a deeper understanding of the factors that influence consumers at an emotional level in order to sustain consumer satisfaction. Evaluating the wine odor-induced emotional response, in addition to hedonic response and descriptive analysis, may provide valuable information into the perspective of the consumer and potentially insight to repeat purchases. The objectives of this research were to measure the emotional responses elicited by odor attributes in wine samples and determine whether these emotional responses vary as function of cultural and experiential backgrounds, as well as sensory characteristics of wine …

Central Administration Of Neuropeptide Y (Npy) And Vasotocin 4 Receptor (Vt4r/V1ar) Antagonists On Food Intake And A Stress Response In Chicks, Megan Ann Hancock

Graduate Theses and Dissertations

The objective of this study was to determine the central effect of the VT4R antagonists (SR-49059 and H-5350 (Manning compound)) on corticosterone (CORT) levels during stress and food intake. A 22-gauge stainless steel guide cannula was surgically implanted into the lateral ventricle of the birds. A preliminary study was done to test the antagonists and their role on food intake. Birds were injected with saline, NPY (4µg), NPY (4µg)+SR-49059 (250ng), or NPY (4µg)+Manning compound (250ng). Birds injected with saline had the lowest 1h food intake (17.7g1.6). Birds injected with NPY+saline had a significantly higher intake (27.1g 1.0), which was enhanced …

A Portable Impedance Biosensing System Based On A Laptop With Labview For Rapid Detection Of Avian Influenza Virus, Yixiang Wang

Graduate Theses and Dissertations

Avian Influenza Virus (AIV) H5N1 is a highly pathogenic virus found not only in birds but also in human. Rapid and sensitive detection method is needed to help prevent the spread of AIV H5N1. In this study, a portable impedance biosensing system based on a laptop with LabVIEW software was developed for detection of AIV H5N1. First, a virtual instrument was programmed with LabVIEW software to form a platform for impedance measurement, data processing and control. The audio card of a laptop was used as a function generator while a data acquisition card was used with the signal channels for …

Price Discovery And Futures Spreads For U.S. And Chinese Rice Futures Market, Wei Yang

Graduate Theses and Dissertations

Rice, the primary staple food for more than half the world's population, is the second world's most consumed food grain. In recent years, world rice price has increased and become more volatile especially in the period 2007-2010. Rice price volatility has a huge impact on Asian countries, especially countries in Southeast Asia where rice is a staple food for millions of households. Private market tools to manage price risk and discover price such as futures markets have been analyzed and assessed as possible solutions to coping with rice price volatility issue. Two primary functions of agricultural commodities futures markets are …

Associations Between Pqct-Based Fat And Muscle Area And Density And Dxa-Based Total And Leg Soft Tissue Mass In Healthy Women And Men, Vanessa D. Sherk, Robert S. Thiebaud, Zhaojing Chen, Murat Karabulut, So Jung Kim, Debra A. Bemben

Health & Biomedical Sciences Faculty Publications

Peripheral Quantitative Computed Tomography (pQCT) can be used for muscle and fat area and density assessments. These may independently influence muscle and fat mass measurements from Dual Energy X-ray Absorptiometry (DXA).

Objective: To determine associations between pQCT-derived soft tissue density and area measures and DXA-derived soft tissue mass.

Methods: Linear regression models were developed based on BMI and calf fat and muscle cross-sectional area (FCSA and MCSA) and density measured by pQCT in healthy women (n=76) and men (n=82) aged 20-59 years. Independent variables for these models were leg and total bone-free lean mass (BFLM) and fat mass (FM) measured …

The Interaction Of Aeolian And Fluvial Processes In Dry Washes On The Colorado Plateau, Usa, Beau Jensen Walker

Theses and Dissertations

In the past decade there has been a call for integrated studies that examine the interaction of fluvial and aeolian processes (Belnap et al., 2011; Bullard and Livingstone, 2002). In this study, we examined the role of land-use, weather, and soil type on the flux of aeolian material into dry washes on the Colorado Plateau in central Utah, USA, and western Colorado, USA. Our goal was to quantify the impact of individual deposition and erosion events by correlating weather and land-use data with a combination of measurement methods including dust collection via dust traps, GPS surveying, and close-range photogrammetry. Our …

Fabrication, Characterization, Optimization And Application Development Of Novel Thin-Layer Chromatography Plates, Supriya Singh Kanyal

Theses and Dissertations

This dissertation describes advances in the microfabrication of thin layer chromatography (TLC) plates. These plates are prepared by the patterning of carbon nanotube (CNT) forests on substrates, followed by their infiltration with an inorganic material. This document is divided into ten sections or chapters. Chapter 1 reviews the basics of conventional TLC technology. This technology has not changed substantially in decades. This chapter also mentions some of the downsides of the conventional approach, which include unwanted interactions of the binder in the plates with the analytes, relatively slow development times, and only moderately high efficiencies. Chapter 2 focuses primarily on …

Role Of Epistasis In Alzheimer's Disease Genetics, Mark T. Ebbert

Theses and Dissertations

Alzheimer's disease is a complex neurodegenerative disease whose basic etiology and genetic structure remains elusive, despite decades of intensive investigation. To date, the significant genetic markers identified have no obvious functional effects, and are unlikely to play a role in Alzheimer's disease etiology, themselves. These markers are likely linked to other genetic variations, rare or common. Regardless of what causal mutations are found, research has demonstrated that no single gene determines Alzheimer's disease development and progression. It is clear that Alzheimer's disease development and progression are based on a set of interactions between genes and environmental variables. This dissertation focuses …

Targeted Sequencing Of Plant Genomes , Mark D. Huynh

Theses and Dissertations

Next-generation sequencing (NGS) has revolutionized the field of genetics by providing a means for fast and relatively affordable sequencing. With the advancement of NGS, whole- genome sequencing (WGS) has become more commonplace. However, sequencing an entire genome is still not cost effective or even beneficial in all cases. In studies that do not require a whole-genome survey, WGS yields lower sequencing depth and sequencing of uninformative loci. Targeted sequencing utilizes the speed and low cost of NGS while providing deeper coverage for desired loci. This thesis applies targeted sequencing to the genomes of two different, non-model plants, Artemisia tridentate (sagebrush) …

Extent Of Cysteine Modification Of Snap-25 In Vitro, Alex Mcgregor Dabell

Theses and Dissertations

Exocytosis, the fusion of a vesicle to a cellular membrane, involves a protein named SNAP-25. This protein, containing two alpha helices connected with a linker region, is localized to the cell membrane via palmitic acids attached to the cysteine residues of its linker region in a process called palmitoylation. Are cysteine residues of the SNAP-25 linker region palmitoylated in an ordered manner and to a particular extent? The answer to this question may give insight into the regulated nature of exocytosis. While it is generally accepted that SNAP-25 must be palmitoylated in order to perform its exocytotic functions, the details …

Molecular Mapping Of Qtls For Domestication-Related Traits In Cowpea (V. Unguiculata (L.) Walp.), Mebeaselassie Andargie, Remy S. Pasquet, Bhavani S. Gowda, Geoffrey M. Muluvi, Michael P. Timko

All Peer-Reviewed Publications

Understanding the genetic basis underlying domestication-related traits (DRTs) of cowpea (Vigna unguiculata (L.) Walp.) is important since the genome has experienced divergent domestication and in addition it is also useful to utilize the wild germplasm efficiently for improving different traits of the cultivated cowpea. Quantitative trait loci (QTLs) for DRTs were identified in a population of 159 F7 recombinant inbred lines derived from a cross between a domesticated cowpea (V. unguiculata (L.) Walp.) variety, 524B, and a wild accession, 219. Using the constructed linkage map, QTLs for 10 DRTs were analysed and mapped. QTLs for seed, pod and flower related …

Studies On The Initial Stage Of Lipid Oxidation In Bulk Oils, Elizabeth Budilarto

Dissertations

During the oxidation of bulk oils, oxidation products (i.e. peroxide values, conjugated dienes and thiobarbituric acid reactive substances) are formed gradually and increased sharply at the end of the induction period. Tocopherols were consumed, some water was formed, and micelles increased in size during the induction period of vegetable oils oxidized in bulk. The evidence that the evolution of micellar size was in parallel with the end of induction period corroborates the recognition that micelles are the active site of oxidation. The interactions of α-tocopherol and three synergists: ascorbyl palmitate, phosphatidylcholine and L-lysine were studied in cod liver oil, to …

Studies On The Initial Stage Of Lipid Oxidation In Bulk Oils, Elizabeth Budilarto

Theses

During the oxidation of bulk oils, oxidation products (i.e. peroxide values, conjugated dienes and thiobarbituric acid reactive substances) are formed gradually and increased sharply at the end of the induction period. Tocophero1s were consumed, some water was formed, and micelles increased in size during the induction period of vegetable oils oxidized in bulk. The evidence that the evolution of micellar size was in parallel with the end of induction period corroborates the recognition that micelles are the active site of oxidation. The interaction of α-tocophero1 and three synergists: ascorby1 palmitate, phosphatidy1choline and L-lysine were studied in cod liver oil, to …

Effect Of Abscisic Acid On The Growth And Development Of Lettuce (Lactuca Sativa 1.) Under Varied Irrigation Regimes, Mohamed Abdulla Al Muhairi

Theses

Economically important vegetable crop lettuce (Lactuca sativa L.) of family Asteraceae was selected for the present investigation. It is being cultivated in UAE due to its commercial importance. In lettuce cultivation, major problem is the requirement of large quantity of irrigation water. The present study was aimed to reduce the water consumption of lettuce cultivation, for that, a varied irrigation regime was used with the application of abscisic acid (ABA). The parameters studied were growth, photosynthetic pigments, biochemical constituents, antioxidant potential and antioxidant enzymes activities in lettuce plants under drought stress and its response to ABA under stress. Drought stress …

Numerical Simulations Of In Vitro Nanoparticle Toxicity – The Case Of Poly(Amido Amine) Dendrimers., Marcus Maher, Pratap Naha, Sourav Prasanna Mukherjee, Hugh Byrne

Articles

A phenomenological rate equation model is constructed to numerically simulate nanoparticle uptake and subsequent cellular response. Polyamidoamine dendrimers (generations 4-6) are modelled and the temporal evolution of the intracellular cascade of; increased levels of reactive oxygen species, intracellular antioxidant species, caspase activation, mitochondrial membrane potential decay, tumour necrosis factor and interleukin generation is simulated, based on experimental observations.

The dose and generation dependence of several of these response factors are seen to well represent experimental observations at a range of time points. The model indicates that variations between responses of different cell-lines, including murine macrophages, human keratinocytes and colon cells, …

The Influence Of Observational Learning On Self-Reported Physical Activity, Self-Efficacy For Physical Activity, And Health-Related Fitness Knowledge For Physical Activity, Charles Gregory Bullock

Dissertations

The obesity epidemic has caused tremendous burden to our economy and healthcare system. Physical activity is one method that can reduce the obesity rate. However, physical activity declines in high school and does not recover. The likelihood of adolescents continuing their involvement in physical activity depends on how they navigate the highs and lows of their physical activity experiences (Feltz & Magyar, 2006). The purpose of this study is to look at the role of observational learning in physical activity and behaviors in an adolescent population. Specifically, this research examines the influence of observational learning on self-reported physical activity, self-efficacy …

Characterization And Role Of Msaabcr In Biofilm Development And Virulence In Staphylococcus Aureus, Gyan Sundar Sahukhal

Dissertations

Community-acquired, methicillin-resistant Staphylococcus aureus strains often cause localized infections in immune-compromised hosts, but some strains show enhanced virulence leading to severe infections even among healthy individuals with no predisposing risk factors. The genetic basis for this enhanced virulence has yet to be determined. S. aureus possesses a wide variety of virulence factors, the expression of which is carefully coordinated by a variety of regulators. Several virulence regulators have been well characterized, but others have yet to be thoroughly investigated. Previously, the msa gene as a regulator of several virulence genes, biofilm development, and antibiotic …

Postural Sway And Brain Hemispheric Power Spectral Density Under Different Attentional Focus Conditions, Sharon Jalene

UNLV Theses, Dissertations, Professional Papers, and Capstones

The purpose of this study was to determine the differences, if any, in the root mean square error (RMSE) of postural sway and hemispheric power spectral density (PSD) in the alpha and beta bands (8-12Hz and 12-25Hz) during different conditions of attentional focus (i.e., internal focus (IF), and external focus (EF) and a control condition (C)). Previous studies have shown that the adoption of IF and EF significantly alter motor performance and that EF promotes automaticity (Wulf, 2013). Sports performance and balance studies utilizing EEG spectral analyses report increased alpha oscillations during expert performance and stable balance and increased beta …

Relationship Between Stream Velocity & Depth And Snail Size Distribution & Density Of The Balcones Elimia, Elimia Comalensis (Pilsbry, 1890) (Gastropoda: Pleuroceridae) In Comal Springs, Texas, Kathryn E. Perez, Russell L. Minton, Scott A. Reese, David M. Haye

School of Integrative Biological & Chemical Sciences (Formerly Dept. of Biology)

Pleurocerid snails are important components of aquatic ecosystems and the majority of species are threatened or endangered. This study describes aspects of the life-history of Elimia comalensis, specifically population density in relationship to water velocity and depth, and seasonal change. Also examined are spatial segregation of different E. comalensis age groups and the relationship of snail size (proxy for age) to 110w, depth, and seasonal change. The study was carried out in the lotic portion of spring run 3, Coma! Springs, New Braunfels, Texas, by quadrat sampling at 10 in intervals from the spring head to Landa Lake in Fall. …

Preliminary Transcriptome Analysis And Homeobox Gene Isonlation For Tomocerus Minor (Entomobryomorpha: Tomoceridae), Alejandro Alaniz

Theses and Dissertations - UTB/UTPA

Collembola, or “springtails,” are small, edaphic arthropods classified as Entognatha; a group traditionally included as part of Hexapoda and sister-group to the true insects. However, some genetic studies suggest that they may instead have derived from a separate crustacean lineage rather than from a proto-Insecta lineage. The unique abdominal structures that develop on the collembolan ventral abdomen appear to be appendicular in origin – atypical to the canonical, appendage-less insect abdomen ground plan. I developed culture and embryo fixation protocols for; and sequenced and analyzed a multi-stage embryo transcriptome for Tomocerus minor (Collembola: Entomobryomorpha). This analysis annotated 14,417 distinct genes, …

Summary Of Raptor Banding Efforts At Hitchcock Nature Center, Fall 2014, Jerry Toll

Nebraska Bird Review

The 2014 season was by far the most unusual and frustrating season since the inception of raptor banding and hawk watch at the Hitchcock Nature Center in Pottawatomie County, Iowa, just north of Crescent. Banding diurnal raptors commenced on September 7th on schedule. The month of September was typical as far as capture rates and weather systems, although we were stymied somewhat by the lack of availability of European Starlings for lure birds. House Sparrows proved a nearly adequate substitute.

During the peak period of October, weather systems that drive diurnal raptor migration worked against monitoring efforts by forcing migration …