Life Sciences Commons^™

The Effects Of Poultry Litter Biochar And Water Source On Radish Growth And Nutrition, Julia Marie Allen

Crop, Soil and Environmental Sciences Undergraduate Honors Theses

Many row-crop fields today have declined in soil fertility due to poor management practices and overuse of pesticides. Under these conditions, plant nutrient uptake can be sub-optimal. There are several soil amendments that can be used to improve soil quality and plant growth such as traditional fertilizers and manure applications. This study focused on the addition of biochar to the soil and the use of structured water to enhance plant growth. Biochar is a nutrient rich product that is produced by pyrolysis of organic feedstocks and contains high rates of carbon. Previous studies which focused on biochar have shown an …

So You Want To Produce Your Own Eggs?, Jacquie Jacob, Anthony J. Pescatore

Agriculture and Natural Resources Publications

Backyard chicken flocks are becoming popular throughout the country in urban, suburban and rural communities. Preparation is essential for a successful backyard flock.

Fern Community Reassembly In Secondary Forests Of Puerto Rico: Predictors, Complexity, And Niche Model Assessment, Thomas J. Schmidt

Masters Theses

Approximately 94% of Puerto Rico’s forests were converted into agricultural systems by 1950. Since then, extensive abandonment of agricultural land has resulted in a considerable amount of forest regeneration throughout the main island. Ferns are a major non-woody component of oceanic, tropical island forests comprising up to seventy percent of the flora. Consequently, the composition and community structure of ferns may be indicative of the relative richness of these secondary forests. I used Maximum Entropy (Maxent), a widely-used mathematical tool for distinguishing suitable versus unsuitable fern niche space, along with ENMTools, a tool that assists Maxent with proper model selection, …

Dietary Behaviors And Nutrition Intervention Preferences Of Breast Cancer Survivors, Sherry A. Stokes

All Theses

Breast cancer is the most commonly diagnosed cancer among women and there are expected to be 1,665,540 new cases diagnosed in 2014. Advancements in early detection, have greatly improved treatment outcomes for the majority of these patients and the 5-year survival rate is 89.2%. The newly emerging field of survivorship care is an attempt to meet the unique needs of this expanding population. Research has demonstrated that an unhealthy body weight is an important modifiable risk factor for the recurrence of breast cancer. This study used an electronic version of the Block 2005 to collect dietary information on 30 breast …

The Effects Of Probiotics Supplementation On Health Using Caenorhabditis Elegans As A Model System, Miranda Klees

All Theses

The 'Western Diet,' prominent among developed nations, often refers to a diet rich in meat proteins and refined sugars. Western society may in part be plagued with obesity and obesity related diseases due to a diet enriched with glucose. With an increasing glycemic index observed in Western society, it comes to no surprise that obesity, diabetes, heart disease, and other obesity related diseases are on the rise. The Centers for Disease Control and Prevention describes obesity as an epidemic, affecting more than 35% of U.S. citizens. Shortened lifespan and increased susceptibility to pathogens are associated with these diseases and linked …

The Effects Of Conjugated Linoleic Acid Supplementation On Lipopolysaccharide-Challenged Synoviocytes, Allison Millican

All Theses

Osteoarthritis is one of the leading causes of lameness in the athletic horse. Cases of equine osteoarthritis typically present with synovitis, or inflammation of the synovium. This inflammation in the synovium leads to the production of inflammatory cytokines and eicosanoids. Although these downstream products can be beneficial, overproduction can lead to degradation of cartilage and joint disease. Opportunities to help mitigate or decrease inflammation within the joint are of current interest to the equine industry. One method being investigated is nutritional supplementation. The anti-inflammatory effects demonstrated by CLA supplementation make it a potential prospect for utilization in mitigating joint inflammation. …

Prevalence Of Human Noroviruses In Commercial Retail Food Establishment Without A Recently Reported Outbreak Of Gastroenteritis, Chaoyi Tang

All Theses

Human noroviruses (NoV) are a leading cause of acute gastroenteritis, causing nearly 58% of foodborne disease in the USA. The aim of our study was to determine the prevalence of NoV on select bathroom surfaces in food establishments in South Carolina (SC). All SC counties (N=46) were sorted by population density into high-, medium-, and low-density categories. Three counties were randomly selected from each population category. Lists of all foodservice establishments in each selected county (N=9) were obtained from the Department of Health and Environmental Control and a total of 120 foodservice establishments were randomly selected. Swabs were collected from …

Arsenic Targets Neural Plate Border Specifier Cells In P19 Cells, Christopher Mccoy

All Theses

Epidemiological studies have shown that arsenic exposure during early embryogenesis can cause reduced weight gain and neurological deficits later on in life. In addition, in vitro and in vivo studies have indicated that arsenic suppresses neurogenesis and myogenesis. The exact mechanism of how arsenic causes these undesired developmental outcomes is poorly understood, however both skeletal muscle and sensory neuron development require the Wnt/β-catenin signaling pathway to initiate the specific differentiation of precursor cells. We were interested in determining the target cell population of arsenic and its metabolites. Arsenic's metabolites were of interest because they have been shown to be more …

Inhibition Of Vibrio Cholerae Biofilm Formation By Water-Soluble Cranberry Extracts, Daniel Pederson

All Theses

Vibrio cholerae, a Gram-negative bacterium found in natural aquatic environments, is the causative agent of cholera, a severe diarrheal disease most commonly spread through drinking water. An essential component to this pathogen's success and persistence in the environment is its ability to attach to both biotic and abiotic surfaces via biofilm formation. Biofilms not only aid in surface attachment, but also provide a barrier that protects and enhances survival. Water-soluble extracts from the North American cranberry (Vaccinium macrocarpon) were found to dramatically inhibit V. cholerae biofilm formation at a low concentration of 2mg/ml. This inhibition was not due to acidification …

Adaptation Of Soil Judging To Northeast China, He Yun

All Theses

Soil Judging teaches students important skills for field identification of soil types, properties, and interpretations for use. The adaptation of Soil Judging in Northeast China can be beneficial to students as well as government agencies and the private sector. The objective of this study was to adapt Soil Judging to the Northeast region of China by a graduate student from China, who was trained using an undergraduate course in Soil Judging and a regional Soil Judging competition. Unlike the U.S., China has 14 soil orders, with six soil orders somewhat similar to the ones found in the Southeast region of …

Liminal, Nina Kawar

All Theses

Throughout life everyone experiences both physical and psychological pains and adversities. In time, the body, mind and spirit are capable of healing. It is within this liminal space between infliction and renewal that the self endures an elusive process that is part of the human condition. Within my installation I have constructed a metaphor for the physical and psychological stages of healing through form, materials, color and process. The spatial environment evokes the literal and metaphorical notion of restoration through a visual, olfactory and physical experience. As the viewer navigates the space, it is the fragmentation and suggestion of form …

Relationship Between Peel Force, Opening Force, And Burst Force For A Semi-Rigid Cup And Lid, Pre- And Post-Retort, Raj Navalakha

All Theses

This research determines if there is any relationship between the peel force, the opening force and the burst force for a semi-rigid cup and lid system, for pre- and post-retort conditions. It also compares the relationship (regression lines) between these forces pre- and post-retort. These seal results were studied by varying the sealing parameters of dwell time and temperature while keeping the pressure constant. Polypropylene cups and a peelable barrier retort lidding were used in this study. As compared to past research, a different peel testing technique was used to measure the peel and the opening force. The entire lid …

An Almost Ideal Demand System Analysis Of Orange And Grapefruit Beverage Consumption In The United States, Catrice Taylor

All Theses

This thesis estimates a demand system for natural fruit juices and flavored citrus drinks. Consumption and price data from the Florida Department of Citrus is used to estimate a Linear Approximate Almost Ideal Demand System for natural fruit and fruit juice drink elasticities. The monthly data covers the period from October 2004 to June 2014. Elasticities show orange juice does not have a substitute among the other beverages. 100% orange juice and grapefruit juice are compliments, although other studies have found them to be substitutes.

Screening St. Augustinegrass For Usda Zone 7, Nathaniel Gambrell

All Theses

St. Augustinegrass [Stenotaphrum secundatum (Walt.) Kuntze] generally has poor cold tolerance yet excellent shade tolerance. As mostly hot summers follow cold winters in USDA Hardiness Zone 7, severely damaging tall fescue [Festuca arundineacea Schreb.] and centipedegrass [Eremochloa ophiuroides (Munro) Hack.], a St. Augustinegrass cultivar cold tolerant enough to be grown for shady lawns would greatly benefit both home owners and sod growers in USDA Hardiness Zone 7. Eight St. Augustinegrass samples were selected, including industry standards 'Raleigh' and 'Palmetto', for further testing from an established germplasm collection of material collected from lawns grown in USDA Hardiness Zone 7. …

Determining The Fate Of Non-O157 Shiga Toxin-Producing Escherichia Coli In Dairy Compost During Storage Using The Optimized Detection Method, Hongye Wang

All Theses

Shiga toxin-producing Escherichia coli (STEC), a member of Enterobacteriaceae family, has been recognized as emerging pathogens. Dairy compost is commonly applied to farmland as a soil amendment. Despite the agricultural benefit of manure-based soil amendment, the inadequately treated compost can contribute to fresh produce contamination on the farm. Moreover, the epidemiological results showed that the non-O157 STEC cases have surpassed those of E. coli O157. Therefore, it is critical to evaluate the behavior of non-O157 STEC strains in the dairy manure-based compost. The objectives of this study were to: 1) optimize a culturing method for detecting non-O157 STEC from dairy …

Characterization Of Biofilms On Medical Device Materials With Application To Reusable Surgical Instruments, Amanda Macaluso

All Theses

Reusable medical devices or reprocessed single-use devices are original medical devices that have been used once and then are cleaned, sterilized, and remanufactured for the purpose of an additional single use on a single patient [4]. Improperly reprocessed devices are a significant contributor to hospital-associated infections [4]. Challenges that hinder reprocessing are related to the complexity of reusable medical device design, the necessary validation of cleaning protocols required by the U.S Food and Drug Administration (FDA), the impact of human factors throughout the reprocessing cycle, as well as economic factors within new business models that are centered on reprocessing. Current …

Combining Modified Atmosphere Packaging And Nisin To Extend The Shelf Life Of Atlantic Salmon (Salmo Salar), Dong Han

All Theses

Atlantic salmon is often used in laboratory experiments to represent marine fishes. Many researchers try to optimize the levels and dosage of preservatives to maintain the freshness and extend the shelf-life of Atlantic salmon. Little information has been provided about the effectiveness of combined method between MAP and nisin, especially on the preservation of Atlantic salmon. Strong evidences suggest that MAP associated with nisin may have a potential to improve Atlantic salmon storage quality. Preservation effectiveness of combining modified atmosphere package (MAP) and nisin on fresh Atlantic salmon was determined using various methods. Six groups of farmed Atlantic salmon were …

Pilot Study Of A Budget-Tailored Culinary Nutrition Education Program For Undergraduate Food Science Students, Dorothy Kerrison

All Theses

The primary objective of this pilot study is to provide evidence that a budget-tailored culinary nutrition program is both appropriate and applicable to undergraduate food science students both in everyday life as well as their future health careers. Two validated programs were combined into one program in order to evaluate their combined effects: Cooking With a Chef and Cooking Matters at the Store. The secondary objective of this pilot study is to evaluate the components and reliability of a questionnaire created specifically for this pilot study. A review of past literature was written, which included culinary nutrition as a source …

Hand Hygiene Promotion: An Essential Strategy For Preventing Foodborne Disease In Elementary Schools, Zhangqi Wang

All Theses

Student absenteeism is a pervasive problem in the United States, causing a number of educational, economic, and institutional problems. Hand-hygiene interventions have been reported to be a method for reducing illness-associated student absenteeism. As an increasing number of schools offer self-service, including salad bars and bowls of whole fresh fruit, opportunities for the transmission of foodborne pathogens via hands could possibly increase illness-associated student absenteeism. To address this problem, we conducted a two-phase study. First, we conducted a systematic literature review to evaluate peer-reviewed studies (N=24) that reported an association between hand hygiene interventions and illness-associated absenteeism reduction in elementary …

Online Versus Face-To-Face Biology: A Comparison Of Student Transactional Distance, Approach To Learning, And Knowledge Outcomes, Mary Erin Riggins

Dissertations

Community colleges are among many other institutions increasing course offerings online, but there is still some concern about the quality of online learning. Educator concerns, a lack of empirical evidence on biology courses offered online, and the need for an equal opportunity for education support the need for clarification of the quality of distance education in biology, especially in the community college setting. Student attitudes, approaches to learning, and performance should all be studied in order to formulate a better evaluation of the quality and effectiveness of online courses (Svirko & Mellanby, 2008).

The purpose of this study was to …

Fungal Contribution To Carbon And Nutrient Cycling In A Subtropical Freshwater Marsh, Rong Su

Dissertations

Despite the well-known occurrence of emergent standing litter in freshwater marshes, very little quantitative data is available concerning the functional role and contribution of fungal decomposers to litter decomposition or their impact on ecosystem scale carbon and nutrient cycling. In the present study, microbial respiration and seasonal biomass and production rates of fungal decomposers associated with standing plant litter were examined to assess the quantitative contribution of fungi to both fine scale litter decay processes and large-scale ecosystem carbon flow pathways in a subtropical Typha domingensis and mixed Cladium jamaicense/Juncus roemarianus freshwater marsh, respectively. In addition, the growth …

Suppression Of Neurodegenerative Symptoms Via Suppressor Of Levy Mutation In Drosophila Melanogaster, William Kaputa

Forensic Science Theses

Mutation of the levy gene on chromosome 2 of Drosophila melanogaster has previously been shown to cause temperature-induced paralysis and neurodegeneration. The Suppressor of levy mutation, Su(levy), also on chromosome 2, modulates the effects of the levy mutation and partly rescues the wild-type phenotype. The goal of this research was to determine if Su(levy) mutation alleviates the effects of neurodegeneration caused by mutations associated with Alzheimer’s and Parkinson’s diseases in humans. The mutant genes used were hLRRK2, α-Synuclein, DJ1-α, Pink, Parkin, and Aβ-42. To determine this, flies carrying the above-mentioned mutant genes were …

Genetic Health And Population Viability Of Reintroduced American Marten In Michigan, Tamara L. Hillman

Masters Theses

American marten (Martes americana) were extirpated from Michigan’s Lower Peninsula (LP) in 1911, and subsequently from the Upper Peninsula (UP) in 1939 due to habitat loss and unregulated trapping. The species was later reintroduced in the UP in the mid-1950s, and to the LP in the mid-1980s. Previous research has determined the small founding sizes used in the LP reintroductions have resulted in losses of genetic diversity, while research in the UP has produced discordant results concerning the effects of the reintroduction methods on genetic health and population structure. Since past research of marten in the LP, no …

Predictive Modeling Of Floral Species Richness In Michigan Prairie Fen Communities, Nichole R. Mason

Masters Theses

Prairie fens contain high levels of floral biodiversity, including 19 state threatened or endangered plant species, and are classified as rare and vulnerable communities by the Michigan Natural Features Inventory. The objective of this thesis was to develop multiple-regression (MR) models that reliably predict total, native, and invasive floral species richness for use by conservation organizations. Floral biodiversity surveys were conducted in eight southern Michigan prairie fens during the 2012 growing season. Simple linear regressions between fen size and biodiversity were used to optimize sampling strategy and effort (i.e., number of transects and plots per transect) in surveys conducted in …

Structural Comparison Of Arctic Plant Communities Across The Landscape And With Experimental Warming In Northern Alaska, Jessica L. Gregory

Masters Theses

Understanding vegetation change is central to forecasting the impacts of climate change. Percent cover, determined from a point frame method, is commonly used to monitor vegetation change. Cover is influenced by canopy structure which may change with the size (growth) or number (density) of individual plants. The overarching objective of this project was to document the relationship between vegetation cover and traits representing plant growth and density and determine if these relationships changed with warming. We used regressions and analysis of covariance to detect which of several traits was most strongly related to cover in vegetation at a wet and …

Genetic Structure Of Yellow Perch Populations In Coastal Areas Of Eastern Lake Michigan, Jessica N. Wesolek

Masters Theses

Genetic population substructure is often overlooked because of discontinuities between management and actual population structure as in the case of yellow perch, an ecologically and economically important indigenous fish species in the Laurentian Great Lakes. A knowledge gaps pertaining to the natural history of yellow perch relates to the biological connectivity between nearshore Lake Michigan and drowned river mouth (DRM) lakes, where it remains unclear whether resident yellow perch from Lake Michigan use DRM lakes for spawning or whether DRM lakes contribute to nearshore yellow perch populations in Lake Michigan. I used DNA fingerprinting (genotyping) to explore biological connectivity between …

Evaluation Of Natural Steelhead Recruitment In The Muskegon River, Michigan, Nicholas C. Albrecht

Masters Theses

The lower Muskegon River is one of the most heavily fished rivers in the state of Michigan and is a valuable component of the multi-billion dollar sport fishery in the Great Lakes. Although significant stocking effort has been invested to maintain and improve the steelhead (Oncorhynchus mykiss) fishery in the Muskegon River, natural recruitment has been severely limited due to high summer water temperatures. The goal of this research project was to evaluate the success of a diffuser system installed in 2008 at Croton Dam to moderate high summer water temperatures in the lower Muskegon River. I estimated natural juvenile …

Functional Characterization Of The Arginine Vasotocin 4 Receptor (Vt4r) In Sensory Circumventricular Organs Of The Chicken Gallus Gallus, Nguiessan Alphonse Aman

Graduate Theses and Dissertations

Past studies have shown that the avian vasotocin 4 receptor (VT4R), homologous to the mammalian arginine vasopressin receptor 1a (V1aR/AVPR1A) is involved in immobilization stress. It was not known, however, whether the receptor is also associated with osmotic stress, and if so, what brain regions may be involved. Four treatment groups of chicks were used for the study. One group was subjected to 1h immobilization stress and two groups were administered intraperitoneal injection of 3 M NaCl or 0.15 M NaCl. One additional group served as controls. After 1 h, blood samples were taken for the determination of plasma levels …

Investigation Of Nuclear And Cytoplasmic Functions Of The Dlipin Protein Of Drosophila Melanogaster, Qiuyu Chen

Graduate Theses and Dissertations

Lipin family proteins are highly conserved proteins present in species ranging from mammals to yeast. Lipin 1, the first Lipin gene identified in fatty liver dystrophy (fld) mutant mice, encodes the bifunctional protein Lipin 1, which can serve as an Mg2+-dependent phosphatidic acid phosphatase (PAP) and transcriptional co-regulator. dLipin, the single Lipin ortholog of Drosophila melanogaster, is required in triglyceride synthesis and fat body development. To study the transcriptional co-regulator activity of dLipin, nuclear receptors were screened to find receptors that interact with dLipin. The genetic interaction data indicated that Drosophila hepatic nuclear receptor 4 (HNF4) was a promising candidate …

The Elimination Of Listeria Monocytogenes Biofilms From Stainless Steel Deli Meat Slicer Components By The Use Of Hurdle Technologies, Alexandria Wynne Mertz

Graduate Theses and Dissertations

Listeria monocytogenes growth is of the greatest concern amongst ready-to-eat foods. The United States Department of Agriculture's Food Safety and Inspection Services determined that deli luncheon meats pose the greatest risk of contamination from L. monocytogenes Industrial meat slicers have many removable parts that are connected with sealers and gaskets, which can become worn over time. These spaces cannot be cleaned adequately, therefore are susceptible to bacterial growth. Planktonic cells form biofilms in order to protect the cell from adverse conditions, like during routine cleaning and sanitation. Once a biofilm is formed, the bacteria are much more difficult to eradicate …