Life Sciences Commons^™

Identification Of Eurycea Using Cytochrome B, Karis A. Myers

Senior Honors Theses

Genomic sequencing is a powerful tool that has many applications for research, one of which is in the field of taxonomy and the identification of species. This thesis discusses the mitochondrial gene cytochrome b and its utility in population genetics and identification of larval amphibians. The development of the Polymerase Chain Reaction and primers are an integral part of the modern DNA sequencing process. The Polymerase Chain Reaction is used to amplify a target DNA sequence, and the protocol for this procedure must be optimized for the specific sequence of target DNA. Primers must also be designed and modified for …

Damage To Association Fiber Tracts Impairs Recognition Of The Facial Expression Of Emotion, Carissa Philippi, Shruti Mehta, Thomas Grabowski, Ralph Adolphs, David Rudrauf

Psychology Faculty Works

No abstract provided.

Genetic Dissection Of Triterpenoid Saponin Production In Chenopodium Quinoa Using Microarray Analysis, Derrick James Reynolds

Theses and Dissertations

Quinoa (Chenopodium quinoa Willd.) is an important food crop for subsistence farmers in the Altiplano (high plains) of Peru, Bolivia, and Argentina. Saponins are part of a diverse family of secondary metabolites that are found in high concentrations in the pericarp of many varieties of quinoa. Due to their bitter taste and anti-nutritive properties, saponins must be removed before the quinoa grain is consumed. There are ‘sweet’ varieties of quinoa that have significantly reduced levels of saponin. Previous research suggests saponin production is controlled by a single locus. The major objective of this research was to elucidate the genetic …

Examining The Effects Of Penning On Juvenile Eastern Box Turtles (Terrapene Carolina Carolina), Nicolas Frederick

Theses and Dissertations

Box turtle (Terrapene carolina) populations have been declining over the last several decades, and one major cause is increasing urbanization. As a result of habitat fragmentation, wildlife managers are frequently turning to new and alternative management strategies. Traditional box turtle management has included relocation, which has been met with limited success. This study aims to combine these strategies with another less-studied one: forcing turtles to overwinter on site by penning them in an outdoor enclosure. Two sets of juvenile box turtles were released at the Virginia Commonwealth University Rice Center: one penned on site in a pen for one year, …

Synchrotron X-Ray Visualisation Of Ice Formation In Insects During Lethal And Non-Lethal Freezing, Brent J. Sinclair, Allen G. Gibbs, Wah Keat Lee, Arun Rajamohan, Stephen P. Roberts, John J. Socha

Biological Sciences Faculty Research & Creative Works

Although the Biochemical Correlates of Freeze Tolerance in Insects Are Becoming Well-Known, the Process of Ice Formation in Vivo is Subject to Speculation. We Used Synchrotron X-Rays to Directly Visualize Real-Time Ice Formation at 3.3 Hz in Intact Insects. We Observed Freezing in Diapausing 3rd Instar Larvae of Chymomyza Amoena (Diptera: Drosophilidae), Which Survive Freezing If It Occurs above 214uC, and non-Diapausing 3rd Instar Larvae of C. Amoena and Drosophila Melanogaster (Diptera: Drosophilidae), Neither of Which Survive Freezing. Freezing Was Readily Observed in All Larvae, and on One Occasion the Gut Was Seen to Freeze Separately from the Haemocoel. There …

Culturally Speaking: Ethnic Dairy Foods, Phillip S. Tong

Dairy Science

No abstract provided.

Overexpression And Partial Purification Of Serine Hydroxymethyltransferase, Kimberly Rose Stevens

Chemistry and Biochemistry

No abstract provided.

Dairy Cow Grouping, Allison Mcmillan

Dairy Science

No abstract provided.

Relationship Of Copper, Zinc And Selenium Status With Udder Health And Mastitis Incidence In The Cal Poly Holstein Herd, Michelle Van Ryn

Dairy Science

No abstract provided.

Lameness And Locomotion Scoring Of Dairy Cows, Haley Lynne Aquino

Dairy Science

No abstract provided.

Algae As An Economical Protein Source For Dairy Cattle Nutrition, Brian Azevedo

Dairy Science

Dairying continues to change with economic and environmental demands of today’s fast moving society. Feed costs continue to rise and now account for a large majority of expenses on dairies; especially protein, which has become expensive to purchase. I believe that Algae can serve as a possible solution to aid in alleviating this problem. Algae grow rapidly and consume undesirable carbon dioxide to release oxygen to the environment, making it an environmentally friendly source of feed. There are particular strands of algae that are high in protein. During the course of this project, I intend to discover how effective protein …

Usage Of The Penn State Forage Separator For Evaluating Particle Size Of Tmrs, Ashley Rene Garcia

Dairy Science

Particle size has many effects on the cow. Adequate forage particle length is necessary for proper rumen function. Dairymen are also having a hard time measuring particle size. Proper particle size distribution of feeds is an important part of ration formulation. Management of forage particle size starts with harvesting forages at the proper stage of maturity. Chopping the crop at the right length helps to achieve the desired particle length in a TMR. Measuring particle length of the forages is only a portion of the solution. Analyzing the TMR particle size is the main goal in measuring the distribution of …

Where The Wild Stuff Grows: Bacteria From The Cow To The Pasture-Izer, Michael Stewart

Dairy Science

No abstract provided.

Two Additional Invasive Scarabaeoid Beetles (Coleoptera: Scarabaeidae: Dynastinae) In Hawaii, Mary Liz Jameson, Darcy E. Oishi, Brett C. Ratcliffe, Grant T. Mcquate

University of Nebraska State Museum: Entomology Papers

Two species of dynastine scarab beetles are reported for the first time on the island of Hawaii: the Pasadena masked chafer, Cyclocephala pasadenae (Casey) (Scarabaeidae: Dynastinae: Cyclocephalini) from North America and Temnorhynchus retusus (Fabricius) (Scarabaeidae: Dynastinae: Pentodontini) from Africa. The Pasadena masked chafer is established on the island of Hawaii, is a recognized pest of turf grass, and is a concern for agriculture and native Hawaiian arthropod species. Temnorhynchus retusus is not known to be an agricultural pest, but its spread on the islands could pose a problem for Hawaiian native species. We provide an overview of each species, characteristics …

Distribution And Diversity Of Archaeal And Bacterial Ammonia Oxidizers In Salt Marsh Sediments, Nicole S. Moin, Katelyn A. Nelson, Alexander Bush, Anne E. Bernhard

Biology Faculty Publications

Diversity and abundance of ammonia-oxidizing Betaproteobacteria (β-AOB) and archaea (AOA) were investigated in a New England salt marsh at sites dominated by short or tall Spartina alterniflora (SAS and SAT sites, respectively) or Spartina patens (SP site). AOA amoA gene richness was higher than β-AOB amoA richness at SAT and SP, but AOA and β-AOB richness were similar at SAS. β-AOB amoA clone libraries were composed exclusively of Nitrosospira-like amoA genes. AOA amoA genes at SAT and SP were equally distributed between the water column/sediment and soil/sediment clades, while AOA amoA sequences at SAS were primarily affiliated with the …

Student Sample Perceptions Of Organic Food Products And Buying Habits, Alycia M. Deus

Agricultural Education and Communication

The purposes of this senior project were to identify college students’ perceptions and knowledge of organic food. This study also focuses on the respondents’ purchasing and budgeting of organic food. This information is essential to inform farmers and ranchers because it serves as an indicator of future market’s demands.

A thirteen item questionnaire was distributed to sixty-five students attending California Polytechnic State University, San Luis Obispo, enrolled in either Agricultural Education and Communication courses or Agricultural Business courses. Both upper and lower division classes were surveyed. The questionnaires were distributed and collected from June 2, 2009 to June 4, 2009. …

Effects Of Zilpaterol Hydrochloride On Growth Rates, Feed Converstion, And Carcass Traits In Calf-Fed Holstein Steers, J. L. Beckett, R. J. Delmore, G. C. Duff, D. A. Yates, D. M. Allen, T. E. Lawrence, N. Elam

Animal Science

Two experiments were conducted to evaluate the effectiveness of zilpaterol hydrochloride (ZH) to enhance growth performance and carcass characteristics in calf-fed Holstein steers. In Exp. 1, Holstein steers (n = 2,311) were fed in a large-pen trial in 2 phases at a commercial feed yard in the desert Southwest. In Exp. 2, a total of 359 steers were fed in a small-pen university study. In Exp. 1 and 2, cattle were implanted with a combination trenbolone acetate-estradiol implant approximately 120 d before slaughter. Cattle were fed ZH for 0, 20, 30, or 40 d before slaughter at a rate of …

Tc17 Cd8 T Cells: Functional Plasticity And Subset Diversity, Adam J. Adler

UCHC Articles - Research

L-17-secreting CD8 T cells (Tc17) have been described in several settings, but little is known regarding their functional characteristics. While Tc1 cells produced IFN-γ and efficiently killed targets, Tc17 cells lacked lytic function in vitro. Interestingly, the small numbers of IFN-γ-positive or IL-17/IFN-γ-double-positive cells generated under Tc17 conditions also lacked lytic activity and expressed a similar pattern of cell surface proteins to IL-17-producing cells. As is the case for Th17 (CD4) cells, STAT3 is important for Tc17 polarization, both in vitro and in vivo. Adoptive transfer of highly purified, Ag-specific IL-17-secreting Tc17 cells into Ag-bearing hosts resulted in near complete …

Lucerne Guidelines For Western Australia Principles For Integrating A Perennial Pasture Into Broadacre Dryland Farming Systems, Diana Fedorenko, Perry Dolling, Christopher Loo, Tom Bailey, Roy Latta

Bulletins 4000 -

Lucerne, often regarded as the queen of forages, has been used in agriculture for centuries. In Western Australia, however, its potential has never been fully realised despite its well-documented ability to dewater soils and reduce groundwater recharge. Over the years adoption has been limited by a range of factors including pests and diseases, soil acidity, grazing management and economics. During the last decade research has addressed these issues, paving the way for much greater impact at the farm and catchment level.

Charge Switch Nucleotides, John G. K. Williams, Gregory R. Bashford, Jiyan Chen, Dan Draney, Nara Narayanan, Bambi Reynolds, Pamela Sheaff

Biomedical Imaging and Biosignal Analysis Laboratory

The present invention provides compounds, methods and systems for sequencing nucleic acid using single molecule detection. Using labeled NPs that exhibit charged-switching behavior, single-molecule DNA sequencing in a microchannel sorting system is realized. In operation, sequencing products are detected enabling real-time sequencing as successive detectable moieties flow through a detection channel. By electrically sorting charged molecules, the cleaved product molecules are detected in isolation Without interference from unincorporated NPs and Without illuminating the polymerase-DNA complex.

Vitisnet: ‘‘Omics’’ Integration Through Grapevine Molecular Networks, Jerome Grimplet, Grant R. Cramer, Julie A. Dickerson, Kathy Mathiason, John Van Hemert, Anne Y. Fennell Dr

Agronomy, Horticulture and Plant Science Faculty Publications

Background: Genomic data release for the grapevine has increased exponentially in the last five years. The Vitis vinifera genome has been sequenced and Vitis EST, transcriptomic, proteomic, and metabolomic tools and data sets continue to be developed. The next critical challenge is to provide biological meaning to this tremendous amount of data by annotating genes and integrating them within their biological context. We have developed and validated a system of Grapevine Molecular Networks (VitisNet). Methodology/Principal Findings: The sequences from the Vitis vinifera (cv. Pinot Noir PN40024) genome sequencing project and ESTs from the Vitis genus have been paired and the …

Lmproving Education Through Brain Research, Lisa J. Ihnen

Master of Education Program Theses

Educational practices are improved because of brain research. Cognitive and behavior sciences have provided important concepts for educational practice. Within recent decades, neuroscience has also contributed to the field of brain research, thus enhancing our understanding oflearning. Three main concepts from neuroscience are explored: synaptogenesis, critical periods, and enriched environments. When the entire body of knowledge from all disciplines of brain research is viewed together, then fmdings can be applied to educational practices.

Vitisnet: ‘‘Omics’’ Integration Through Grapevine Molecular Networks, Jerome Grimplet, Grant R. Cramer, Julie A. Dickerson, Kathy Mathiason, John Van Hemert, Anne Y. Fennell

Agronomy, Horticulture and Plant Science Faculty Publications

Background: Genomic data release for the grapevine has increased exponentially in the last five years. The Vitis vinifera genome has been sequenced and Vitis EST, transcriptomic, proteomic, and metabolomic tools and data sets continue to be developed. The next critical challenge is to provide biological meaning to this tremendous amount of data by annotating genes and integrating them within their biological context. We have developed and validated a system of Grapevine Molecular Networks (VitisNet).

Methodology/Principal Findings: The sequences from the Vitis vinifera (cv. Pinot Noir PN40024) genome sequencing project and ESTs from the Vitis genus have been paired and the …

Phylogeography And Postglacial Dispersal Of Smallmouth Bass (Micropterus Dolomieu) Into The Great Lakes, W. Calvin Borden, Robert A. Krebs

Biological, Geological, and Environmental Faculty Publications

Refugia and dispersal routes of smallmouth bass (Micropterus dolomieu) into the Great Lakes were identified using 427 mitochondrial sequences from across their native range. Overall, smallmouth bass accessed the Great Lakes via long-distance dispersal from multiple refugia, and the distribution of genetic variation reflected the consequences of vicariant, dispersal, and paleogeological events. Proximity of lakes to glacial outlets had a greater predictive power on the resulting distribution of mitochondrial diversity relative to interbasin migration. Populations in the Eastern and Interior Highlands contained the most divergent and oldest haplotypes, reflecting their role as glacial refugia and subsequent high incidence of endemicity. …

Remodeling Of The Tight Junction During Recovery From Exposure To Hydrogen Peroxide In Kidney Epithelial Cells, Jeannette E. Gonzalez, R. J. Digeronimo, D'Ann E. Arthur, Jonathan M. King

Biology Faculty Research

Renal ischemia-reperfusion injury results in oxidative stress-induced alterations in barrier function. Activation of the mitogen-activated protein (MAP) kinase pathway during recovery from oxidative stress may be an effector of oxidant-induced tight junction reorganization. We hypothesized that tight junction composition and barrier function would be perturbed during recovery from oxidative stress. We developed a model of short-term H₂O₂ exposure followed by recovery using Madin Darby canine kidney (MDCK II) cells. H₂O₂ perturbs barrier function without a significant cytotoxic effect except in significant doses. ERK-1/2 and p38, both enzymes of the MAP kinase pathway, were activated …

Communicator, Dec. 2009, San Jose State University, Department Of Kinesiology

Communicator (Kinesiology)

Volume 22, Issue 2

Description Of The Larva Of Lophodiplosis Trifida, An Australian Gall Midge (Diptera: Cecidomyiidae) And Biocontrol Agent Of Paperbark In Florida, Usa, Raymond Gagne, Susan Wright, Matthew F. Purcell, Bradley T. Brown, Paul D. Pratt, Ted D. Center

USDA Systematic Entomology Laboratory

Lophodiplosis trifida Gagné, an Australian gall midge on paperbark, Melaleuca quinquenervia (Myrtaceae), is a recent release in southern Florida for the biological control of that host. The larval stage is described for the first time and compared to that of other Lophodiplosis species. Photos of galls and illustrations of larvae are provided. Second and third instars of L. trifida are unusual among Cecidomyiidae for the lack of setae on most papillae.

Economic Contribution Of The Agricultural Sector To The Arkansas Economy In 2007 And Revised Estimates For 2006, Nathan Kemper, Jennie Popp, Wayne Miller

AAES Research Reports and Research Bulletins

This report is the fourth in a series of reports examining agriculture’s economic contribution on the Arkansas economy. Utilizing data from the United States Bureau of Economic Analysis, USDA Economics Research Service, USDA National Agricultural Statistics Service, and Minnesota IMPLAN Group, Inc., the economic contribution of agriculture on the Arkansas economy was estimated for the most recent year available, 2007. Gross Domestic Product (GDP) by State information for Arkansas was compared with those of other states in the southeast U.S. to give a measure of the relative importance of agriculture in Arkansas. The total economic contribution of agriculture (direct, indirect, …

Remote Sensing Of Harmful Algal Blooms In The Mississippi Sound And Mobile Bay: Modelling And Algorithm Formation, Dan Martin Holiday

Dissertations

The incidence and severity of harmful algal blooms have increased in recent decades, as have the economic effects of their occurrence./The diatom Pseudo-nitzschia spp. caused fisheries closures in Mobile Bay during 2005 due to elevated levels of domoic acid. In the previous 4 years Karenia brevis counts of >5,000 cells L"1 have occurred in Mobile Bay and the Mississippi Sound. Population levels of this magnitude had previously been recorded only in 1996. Increases in human populations, urban sprawl, development of shoreline properties, sewage effluent and resultant changes in NP ratios of discharge waters, and decline in forest and marsh lands, …

Experimental Population Dynamics Of Amyloodiniumocellatum In The Spotted Seatrout, Cynoscion Nebulosus, And The Red Snapper, Lutjanus Campechanus, Ignacio Masson

Dissertations

Amyloodinium ocellatum is a parasitic dinoflagellate that infects warm water marine bony fishes and causes high mortalities in aquaculture settings. It has three life history stages: the feeding trophont, the reproductive tomont, and the infective dinospore. This dissertation describes the characteristics of A. ocellatum infections in juvenile spotted seatrout, Cynoscion nebulosus, and red snapper, Lutjanus campechanus, studies the survival and fecundities of the stages in the life cycle of the parasite and presents a population model for A. ocellatum.

At 25°C and 33 ppt, the peak of trophont detachment occurred on day 2 postinfection in spotted seatrout and day 3 …