Life Sciences Commons^™

Geology And Wine In Missouri: Spatial Analysis Of Terroir Using A Geographic Information System And Remote Sensing, Kathryn Nora Barnard

Graduate Theses/Dissertations

The concept of terroir, based on the French word meaning 'sense of place,' suggests that flavor and quality of wine is associated with certain physical characteristics of the earth as well as viticulture and viniculture practices. The physical characteristics of terroir include soil, geology, topography, and climate. Designated American Viticultural Areas (AVAs) are discrete appellations in the United States that have well-established and historic viticultural practices, but the relationship between terroir and AVA appellations is complex. Wineries are a growing industry in the United States and the use of a GIS and remote sensing has proved useful in many other …

The Influence Of Concurrent Visual Feedback During Treadmill Training In Parkinson’S Disease, Rose Ellen Johnston

Theses and Dissertations (Comprehensive)

Walking is an exercise that has been thought to benefit Parkinson’s disease (PD). In a laboratory setting treadmills have been employed to successfully improve certain aspects of gait in PD (Cakit et al., 2007, Fisher et al., 2008, Herman et al., 2007, Miyai et al., 2000, Miyai et al., 2002, Pohl et al., 2003). Research has also indicated that walking with visual cues can improve aspects of gait that are impaired in PD (Azulay et al., 1999, Bagely et al., 1997, Nieuwboer et al., 2007), however, the integration of visual cues and treadmill training has yet to be studied. Thus, …

Mechanism(S) Of 3-Trifluoromethyl-4-Nitrophenol (Tfm) Toxicity In Sea Lamprey (Petromyzon Marinus) & Rainbow Trout (Oncorhynchus Mykiss), Oana Birceanu

Theses and Dissertations (Comprehensive)

The pesticide, 3-trifluoromethyl-4-nitrophenol (TFM), has been extensively used over the last 50 years to control invasive sea lamprey (P. marinus) populations in the Great Lakes, but its mechanism of toxicity is unresolved. Due to its structural similarity to phenolic compounds known to inhibit mitochondrial ATP production, one hypothesis on the mode of action of TFM is that it impairs mitochondrial oxidative phosphorylation. A second hypothesis is that TFM targets the gill, interfering with gill ion uptake. Exposure of larval sea lamprey and rainbow trout (O. mykiss), to each animal’s respective 12-h TFM LC50, resulted in marked …

Effects Of Estrogen Receptor Activation On Post-Exercise Muscle Satellite Cell Proliferation, Kareem Bunyan

Theses and Dissertations (Comprehensive)

This study investigated the role of estrogen receptor activation on total and proliferating satellite cells following running exercise in rats, by using an estrogen receptor-alpha (ER-α) specific agonist, 4,4,4 (4-Propyl-[1H]-pyrazole-1,3,5-triyl) trisphenol (PPT). PPT is the first ER-α specific agonist (Stauffer et al 2000) which binds the ER-a subtype with a 410-greater affinity than ER-β (Stauffer et al 2000). Previous studies have demonstrated that estrogen can augment muscle satellite cell numbers following exercise (Enns and Tiidus, 2008) and that this augmentation may be due to mechanisms activated via estrogen receptors that can influence total, proliferating, and differentiating satellite cell numbers (Enns …

A Study Of The Waterborne And Dietary Toxicityof Cadmium To Hydra Attenuata And Daphnia Plux In Soft Waters And The Development Of Biotic Ligand Models To Predict Such Toxicity, Matthew S.A. Clifford

Theses and Dissertations (Comprehensive)

The goal of this thesis was to compare the effects of waterborne Cd exposures and dietary Cd exposures to Hydra attenuata. Before the Hydra could be exposed to dietborne exposures of Cd, the effects of waterborne exposures had to be understood. This was done by looking at the sensitivity of D. pulex to Cd and then understanding the accumulation of Cd. After bioaccumulation patterns of Cd. to D. pulex were known, we could then feed the prey to the Hydra and comapre the effects to waterborne effects of Cd to Hydra.

To understand the waterborne effects of Cd to …

Population Density, Circulating Testosterone And Dawn Chorus Behaviour In White-Throated Sparrows (Zonotrichia Albicollis), Simon Richard Hall

Theses and Dissertations (Comprehensive)

An integrative approach to biological research has facilitated the study not only of specific phenomena, but of the connections between these phenomena. A particularly striking example is that of the relationships between testosterone, social behavior, and social context. Increased levels of testosterone increase the frequency of aggressive behaviors and reduce the quality of parental care in many songbird species, and these changes in behavior can affect the surrounding social environment. Different social contexts, population density as a readily studied example, affect the cues that control the release of testosterone into the bloodstream. The distinct tan-stripe and white-stripe phenotypic morphs of …

Using Chemical, Optical And Biological Methods To Characterize Cu Complexation By Different Natural Organic Matter Sources Collected From Canadian Shield Waters, Keegan Andrew Hicks

Theses and Dissertations (Comprehensive)

The effects of natural organic matter (NOM) source on Cu complexation were characterized chemically, optically and biologically in soft waters. NOM from different Canadian Shield soft water lakes were concentrated using reverse osmosis and for comparison NOM was also sampled from a hard water source. NOM complexation capacity was assessed directly in Cu spiked solutions (with and without additions of NOM) by measuring free Cu activity using an ion selective electrode. Additional chemical and optical characterizations of NOM included specific absorbance coefficient (SAC; at 340 nm), excitation-emission matrix spectroscopy, protein content, and molecular weight fractionation (fraction of DOCkDa). The biological …

Piecing Together The Puzzle Of Autism: Aspects Of Its Effects On Children With The Condition And Their Caregivers, Roula Markoulakis

Theses and Dissertations (Comprehensive)

As one of the most prevalent developmental disorders, extensive research exists concerning Autism Spectrum Disorders (ASD). However, gaps are still evident in a number of areas. Information pertaining to any costs and benefits associated with the experience of caring for a child with autism is inadequate. In Study 1, the lived experiences of married female primary caregivers of children with autism were explored, specifically by examining the costs and benefits of caregiving and its influence on the women’s situations. Furthermore, motor control deficits are apparent in many children with high functioning autism (HFA) and Asperger syndrome (AS), but need to …

The Influence Of Propyl Pyrazole Triol On The Post-Exercise Alpha Estrogen Receptor-Mediated Activation Of Satellite Cells In Skeletal Muscle Of Ovariectomized Rats, Amy Elisabeth Thomas

Theses and Dissertations (Comprehensive)

Estrogen has been shown to augment satellite cell activation, proliferation and total number and that this may occur through an estrogen receptor (ER) mediated mechanism. The purpose of this study was to investigate the role of ERs in the postexercise estrogen-induced augmentation of satellite cells in skeletal muscle of ovariectomized rats. Furthermore, the specific role of the ERα was examined through the use of an ERa agonist, propyl pyrazole triol (PPT). Ovariectomized rats were used (n=64) and separated into 4 groups: sham, estrogen supplemented, agonist supplemented and a combined estrogen and agonist supplemented group. These groups were further subdivided into …

Inference, Orthology, And Inundation: Addressing Current Challenges In The Field Of Metagenomics, Gregory Detlev Alexander Vey

Theses and Dissertations (Comprehensive)

The vast increase in the number of sequenced genomes has irreversibly changed the landscape of the biological sciences and has spawned the current post-genomic era of research. Genomic data have illuminated many adaptation and survival strategies between species and their habitats. Moreover, the analysis of prokaryotic genomic sequences is indispensible for understanding the mechanisms of bacterial pathogens and for subsequently developing effective diagnostics, drugs, and vaccines. Computational strategies for the annotation of genomic sequences are driven by the inference of function from reference genomes. However, the effectiveness of such methods is bounded by the fractional diversity of known genomes. Although …

The Relationship Between Socioeconomic Status And Physical Activity Among Adolescents, Meredith Laurel Stockie

Theses and Dissertations (Comprehensive)

Background: Many Canadian youth are inactive (Active Healthy Kids Canada, 2009). Socioeconomic status (SES) is one factor that influences youth physical activity (PA) levels; however, the factors involved in this relationship are not well understood. Given that numerous quantitative studies suggest there is a positive relationship between SES and PA among adolescents, but offer little insight into why the given relationship exists, the purpose of the current thesis is to study the factors that influence the relationship between SES and PA among youth aged 12–14 years through a qualitative lens.

Methods: Low (n = 2) and high SES (n = …

An Examination Of Intercollegiate Athletic Injury Tracking Systems Within Canadian Universities, Andrew D. Ross

Theses and Dissertations (Comprehensive)

Information regarding the practice of athletic injury tracking within Canadian intercollegiate institutions has been relatively deficient. The study aimed to assess the current status of athletic injury tracking systems (AITS) in Canadian universities within the Canadian Interuniversity Sport Association (CIS). A secondary purpose was to obtain data from therapists within Canadian Universities, to identify and analyze issues, benefits, barriers and obtain information regarding how athletic injury tracking methods are conducted. This was achieved by obtaining data regarding the current state of AITS in CIS institutions, clinical demographics, athletic therapists and/or physiotherapists’ opinions regarding the pro’s and con’s of athletic injury …

Metabolic And Molecular Approaches To The Study Of Bacterial Communities In Wetlands Of The Alberta Athabasca Oil Sands Region, Jessica Dawn Morrison

Theses and Dissertations (Comprehensive)

Bacterial communities in wetlands from the Athabasca region (Alberta, Canada) were surveyed and their utility as bio-indicators for wetland reclamation was assessed. Sediment samples were collected from wetlands categorized as: (1) natural (off mining leases), (2) reference (on mining sites but not directly impacted by oil sands processed material (OSPM)), and (3) OSPM (directly affected by OSPM). Wetlands of the latter two groups ranged in age from 11 to 24 years. Analysis involved community level physiological profiling (CLPP) with BIOLOG™ EcoPlates, and denaturing gradient gel electrophoresis (DGGE). Multivariate analysis applied to data from both techniques revealed differences in the bacterial …

Evolution And Systematic Significance Of Reproductive Structures In The Genus Cuscuta (Dodders, Convolvulaceae): Pollen And Gynoecium, Mark Welsh

Theses and Dissertations (Comprehensive)

The genus Cuscuta (dodders, Convolvulaceae) is one of the most significant lineages of parasitic plants from economic, conservation, and anthropogenic perspectives. Members of the genus are twining stem parasites with no roots, lacking almost completely chlorophyll or its function, and gather required nutrition from their host via specialized haustorial connections. While there are almost 200 described species, problems with species identification exist because many diagnostic characters are restricted to their tiny flowers. Probably contributing to this identification difficulty is the fact that the group has not received taxonomic attention for over 75 years; even knowledge of its basic reproductive biology …

Characterization Of The Interaction Between Arabidopsis Toc159 And Toc33 Gtpase Domains, Wesley A. Farquharson

Theses and Dissertations (Comprehensive)

Protein-protein interactions are critical for many biochemical processes and for cellular function. Understanding interactions between proteins and ligands, as well as other proteins are necessary for the modelling of biological systems. Protein import into chloroplasts is one such system where the understanding of interactions between members of the translocation complex is limited. The translocon at the outer envelope membrane of chloroplasts (Toe complex) consists of three core components: Toc75, Tocl59, and Toc34. Members of the Tocl59 and Toc34 gene families in Arabidopsis thaliana have been shown to assemble differentially into structurally and functionally distinct complexes. More specifically, there is evidence …

Oral Rabies Vaccination In North America: Opportunities, Complexities, And Challenges, Dennis Slate, Timothy P. Algeo, Kathleen M. Nelson, Richard B. Chipman, Dennis Donovan, Jesse D. Blanton, Michael Niezgoda, Charles E. Rupprecht

United States Department of Agriculture Wildlife Services: Staff Publications

Steps to facilitate inter-jurisdictional collaboration nationally and continentally have been critical for implementing and conducting coordinated wildlife rabies management programs that rely heavily on oral rabies vaccination (ORV). Formation of a national rabies management team has been pivotal for coordinated ORV programs in the United States of America. The signing of the North American Rabies Management Plan extended a collaborative framework for coordination of surveillance, control, and research in border areas among Canada, Mexico, and the US. Advances in enhanced surveillance have facilitated sampling of greater scope and intensity near ORV zones for improved rabies management decision-making in real time. …

Role Of P33 In Tombusvirus Replication, Jozsef Stork

University of Kentucky Doctoral Dissertations

Replication of the nonsegmented, plus-stranded RNA genome of Cucumber necrosis tombusvirus (CNV) requires two essential overlapping viral-coded replication proteins, the p33 replication co-factor and the p92 RNA-dependent RNA polymerase. In my thesis I describe (i) the effect of phosphorylation of p33, (ii) the RNA chaperone-like activity of p33, and (iii) the role of HSP70s a host proteins in the viral replication. To test the effect of phosphorylation on p33 function, I used in vitro phosphorylated p33. I found that phosphorylation inhibited the ability of p33 to bind to the viral RNA. Phosphorylation-mimicking mutations rendered p33 nonfunctional in plant protoplasts and …

Coordination Of Swimbench Freestyle In Elite And Non-Elite Swimmers: A Dynamical System Approach, Tracy H. Spigelman

University of Kentucky Doctoral Dissertations

Elite swimmers can be distinguished from novice swimmers by freestyle stroke technique. Elite swimmers move through multiple coordination modes, increases in stroke lengths, stroke rates, and body roll allowing for a more symmetrical stroke and increased speed compared with novice swimmer during 100m freestyle.

Coaches strive to improve swimmers’ performance by providing feedback about stroke technique, mostly from the pool deck where view of the full stroke cycle is obstructed by the water. Tools to assess swimming are often expensive and require extra training, which does not provide a pragmatic solution. A dryland rotational swimbench would provide a means to …

Insights Into Expression, Cellular Localization, And Regulation Of Supernatant Protein Factor, A Putative Regulator Of Cholesterol Biosynthesis, Elzbieta Ilona Stolarczyk

University of Kentucky Doctoral Dissertations

SPF (Supernatant Protein Factor) is a cytosolic protein that stimulates at least two enzymes in the cholesterol biosynthetic pathway: squalene monooxygenase and HMGCoA reductase. The mechanism of action has not been established but may be related to lipid transfer between intracellular membranes.

There are three human genes for SPF: SEC14L2 (SPF1), SEC14L3 (SPF2) and SEC14L4 (SPF3). The present study differentiates these closely related genes by evaluating their tissue-specific and relative expression levels. SPF1 mRNA was found to be most abundant in liver, mammary gland and stomach. SPF2 showed negligible expression in all tissues tested; SPF3 expression pattern was similar to …

Hymenopteran Molecular Phylogenetics: From Apocrita To Braconidae (Ichneumonoidea), Barbara J. Sharanowski

University of Kentucky Doctoral Dissertations

Two separate phylogenetic studies were performed for two different taxonomic levels within Hymenoptera. The first study examined the utility of expressed sequence tags for resolving relationships among hymenopteran superfamilies. Transcripts were assembled from 14,000 sequenced clones for 6 disparate Hymenopteran taxa, averaging over 660 unique contigs per species. Orthology and gene determination were performed using modifications to a previously developed computerized pipeline and compared against annotated insect genomes. Sequences from additional taxa were added from public databases with a final dataset of 24 genes for 16 taxa.

The concatenated dataset recovered a robust and well-supported topology; however, there was extreme …

Evaluating The Effects Of Organic And Conventional Inputs On Soil Chemical And Biological Properties In A Four-Year Vegetable Rotation And The Investigation Of Soil Microbial Properties On Plant Gene Expression, Audrey Law

University of Kentucky Doctoral Dissertations

The objective of this research was to determine the effects of conventional inputs on soil chemical and biological properties compared to organic systems in a four year vegetable rotation. Tillage and cover crops were the same in all treatments to avoid confounding factors often present in similar research. Additional experiments investigated plant gene expression in organic and conventional management systems and in soils with decreased microbial diversity. Experimental plots were prepared in the spring of 2004; four replications of three management treatments, organic, low-input and conventional, were arranged in a randomized complete block design. The rotation consisted of edamame soybean, …

Changes In Longitudinally Assessed Biomechanical Parameters Related To Increased Risk Of Anterior Cruciate Ligament (Acl) Injuries In Adolescent Female And Male Athletes, Kevin Ray Ford

University of Kentucky Doctoral Dissertations

Females suffer anterior cruciate ligament (ACL) injuries at a 2 to 10-fold greater rate compared to male athletes participating in similar sports. Altered movement patterns and inadequate knee stiffness are two interrelated factors that may increase ACL injury risk. Onset of these neuromuscular risk factors may coincide with the rapid adolescent growth that results in the divergence of a multitude of neuromuscular parameters between sexes. The overall purpose of this dissertation was to determine if neuromuscular ACL injury risk factors in female athletes increase following rapid growth and development compared to males. Male and female athletes were tested with three-dimensional …

Substrate And Regulation Of Mitochondrial Μ-Calpain, Aashish Joshi

University of Kentucky Doctoral Dissertations

μ -Calpain is localized to the mitochondrial intermembrane space. Apoptosisinducing factor (AIF), which executes caspase-independent cell death, is also localized to the mitochondrial intermembrane space. Following processing at the N-terminus, AIF becomes truncated (tAIF) and is released from mitochondria. The protease responsible for AIF processing has not been established. The same submitochondrial localization of mitochondrial μ-calpain and AIF gives support to the hypothesis that mitochondrial μ-calpain may be responsible for processing AIF. Atractyloside-induced tAIF release in rat liver mitochondria was inhibited by cysteine protease inhibitor MDL28170, but not by calpain inhibitors PD150606 or calpastatin. Moreover, μ-calpain immunoreactivity was difficult to …

Identification And Characterization Of Host Factors Involved In Tombusvirus Replication, Yi Jiang

University of Kentucky Doctoral Dissertations

Positive strand RNA viruses are intracellular parasites, and their genome replication and infection involves complex virus-host interactions. Therefore, identification of host factors and dissection of their functions during virus replication could facilitate our understanding of the mechanism of virus infection. Those host factors may also provide new targets for viral disease control. Tomato bushy stunt virus (TBSV) has recently become one of the model viruses to study positive strand RNA virus replication and hostvirus interactions. To identify host factors involved in TBSV replication we used yeast as a model host. Co-expression of the replication proteins and a replicon RNA (DI …

Biology And Management Of The Bed Bug, Cimex Lectularius L. (Heteroptera: Cimicidae), Alvaro Romero

University of Kentucky Doctoral Dissertations

The near absence of bed bugs from human dwellings for fifty or more years has left us with limited knowledge of its biology and few answers to eliminate populations. I explored a diverse set of objectives to answer key questions concerning bed bug biology and control. Major objectives were studies of circadian rhythmicity, pyrethroid resistance, sublethal effects of insecticides, synergism of pyrethroids, and evaluation of a pyrrole insecticides, chlorfenapyr. Additional studies included persistence of Borrelia in bed bugs after ingestion, and aggregation factors from feces.

In the absence of host stimuli, insects were much more active in the dark than …

The Interplay Between The Expression And Functions Of Wnt13 Isoforms During Apoptosis In Bovine Aortic Endothelial Cells, Tao Tang

University of Kentucky Doctoral Dissertations

Wnt proteins are crucial for development/homeostasis by controlling cell fate including apoptosis (Moon RT et al. 1997). Three humanWnt13 isoforms were identified: the secreted Wnt13A, mitochondrial Wnt13B, and nuclear Wnt13C forms; and nuclear Wnt13 had an increased sensitivity to LPS/TNF-induced apoptosis in primary endothelial cells (EC); both Wnt13B and C mRNA contain two start codons (AUG+1 and +74), but the same protein encoded from AUG+74 by Wnt13C was expressed lower than Wnt13B (Struewing IT et al.2006). We hypothesize that during EC apoptosis, the nuclear Wnt13C expression is regulated translationally; nuclear Wnt13 favors apoptosis through regulating the activity/expression of apoptosis-related factors; …

Transcriptional And Morphological Changes During Thyroxine-Induced Metamorphosis Of The Mexican Axolotl And Axolotl-Tiger Salamander Hybrids, Robert Bryce Page

University of Kentucky Doctoral Dissertations

For nearly a century, amphibian metamorphosis has served as an important model of how thyroid hormones regulate vertebrate development. Consequently metamorphosis has been studied in a number of ways including: morphologically, developmentally, ecologically, and from an endocrine perspective. Over the last two decades, much has been learned about the molecular basis of anuran (frog) metamorphosis. However, very little is known about the molecular underpinnings of urodele (salamander) metamorphosis. Using the axolotl and axolotl hybrids as models, I present some of the first studies on the gene expression changes that occur during urodele metamorphosis. In Chapter 1, the motivation for the …

The Distribution Of Unpaired During Drosophila Oogenesis, Travis Sexton

University of Kentucky Doctoral Dissertations

Janus Kinase (JAK) activity specifies the cell fates of the follicular epithelium during Drosophila oogenesis by establishing a gradient of JAK activity with highest levels at the A/P poles. Unpaired (Upd), a ligand for the pathway, is expressed and secreted exclusively from the polar cells potentially establishing the JAK activity gradient. This project proposed that Upd acts as a morphogen to directly establish the JAK activity gradient, specifying the fates of the follicular epithelium. The aims of this work were to investigate the extracellular distribution of Upd and, in addition, factors that may be involved. Furthermore, upd3, a gene …

Towards Elimination And Genetic Manipulation Of Ergot Alkaloid Production In Fungal Endophytes, Simona Florea

University of Kentucky Doctoral Dissertations

Clavicipitaceous fungal endophytes provide several ecological benefits to their hosts. Besides improving host’s growth characteristics, Neotyphodium coenophialum, the endophyte of tall fescue (Lolium arundinaceum), produces ergot alkaloids that have been proposed to be involved in fescue toxicosis. One approach to address the toxicosis problem is to genetically manipulate and modify N. coenophialum by knocking out a pair of homologous genes, (dmaW1 and dmaW2), encoding dimethylallyltryptophan synthase, the enzyme for the first and determinant step in ergot-alkaloid biosynthesis. In this study, disruption of dmaW2 was attempted using several disruption methods. Out of 1522 transformants screened, three …

Improving Follow-Up Of Abnormal Cancer Screens Using Electronic Health Records: Trust But Verify Test Result Communication, Hardeep Singh, Lindsey Wilson, Laura A Petersen, Mona K Sawhney, Brian Reis, Donna Espadas, Dean F Sittig

Faculty, Staff and Student Publications

BACKGROUND: Early detection of colorectal cancer through timely follow-up of positive Fecal Occult Blood Tests (FOBTs) remains a challenge. In our previous work, we found 40% of positive FOBT results eligible for colonoscopy had no documented response by a treating clinician at two weeks despite procedures for electronic result notification. We determined if technical and/or workflow-related aspects of automated communication in the electronic health record could lead to the lack of response.

METHODS: Using both qualitative and quantitative methods, we evaluated positive FOBT communication in the electronic health record of a large, urban facility between May 2008 and March 2009. …