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2007

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Articles 3241 - 3270 of 3598

Full-Text Articles in Life Sciences

Isolation Of Salmonella Enterica And Shiga-Toxigenic Escherichia Coli O157 From Feces Of Animals In Public Contact Areas Of United States Zoological Parks, James E. Keen, Lisa M. Durso, Thomas P. Meehan Jan 2007

Isolation Of Salmonella Enterica And Shiga-Toxigenic Escherichia Coli O157 From Feces Of Animals In Public Contact Areas Of United States Zoological Parks, James E. Keen, Lisa M. Durso, Thomas P. Meehan

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

The fecal prevalence of subclinical Salmonella enterica and Shiga-toxigenic Escherichia coli O157 among animals in human-animal contact exhibits at institutions in the United States accredited by the Association of Zoos and Aquariums was estimated to assess public health risk. The prevalence was less than 0.6% for both zoonotic pathogens among 997 animals sampled at 36 exhibits.


A Cellulose Fiber-Based Diet For Screwworm (Diptera: Calliphoridae) Larvae, M. F. Chaudhury, Steven R. Skoda Jan 2007

A Cellulose Fiber-Based Diet For Screwworm (Diptera: Calliphoridae) Larvae, M. F. Chaudhury, Steven R. Skoda

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

A highly absorbent cellulose fiber from recycled paper was tested and compared with a polyacrylate gelling agent, Aquatain, normally used for bulking and solidifying larval rearing medium of screwworm, Cochliomyia hominivorax (Coquerel) (Diptera: Calliphoridae). The absorbent fiber, when mixed with water and dietary ingredients, produced a diet medium of homogeneous texture that supported larval growth and development comparable with the standard gelled diet. Larval and pupal weights from two concentrations of cellulose fiber-based diet were significantly higher than those obtained using gelled diet. The number of pupae per tray, percentage of adult emergence, oviposition, percentage of egg hatch, and adult …


Lixus Cardui, A Biological Control Agent For Scotch Thistle (Onopordum Acanthium): Safe For Australia, But Not The Usa?, Joe Balciunas Jan 2007

Lixus Cardui, A Biological Control Agent For Scotch Thistle (Onopordum Acanthium): Safe For Australia, But Not The Usa?, Joe Balciunas

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Invasive exotic plants are often weeds in more than one country. After a biological control agent for a weed has been developed for use in one country, it is reasonable to consider using the same agent against the same weed in another country. ‘Transfer Projects’ can save considerable time and money, and they have been popular around the world. Lixus cardui Olivier (Coleoptera: Curculionidae), a weevil from Europe, was first used by Australian researchers to control Scotch thistle, Onopordum acanthium L. (Asteraceae). There are few close relatives of Scotch thistle in Australia, but that is not the case in North …


Physiological Host Range Of Ceratapion Basicorne, A Prospective Biological Control Agent Of Centaurea Solstitialis (Asteraceae), Lincoln Smith Jan 2007

Physiological Host Range Of Ceratapion Basicorne, A Prospective Biological Control Agent Of Centaurea Solstitialis (Asteraceae), Lincoln Smith

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Ceratapion basicorne (Coleoptera: Apionidae) is a weevil native to Europe and western Asia that is being evaluated as a prospective classical biological control agent of Centaurea solstitialis (yellow starthistle) in the United States. Host plant specificity of the insect was evaluated in no-choice oviposition experiments. Feeding on leaf tissue by adult females was highly correlated to oviposition rate, both of which occurred primarily on plants in the tribe Cardueae, and especially those in the monophyletic subtribe Centaureinae. The highest rates of larval development occurred on Ce. solstitialis and Centaurea cyanus (bachelor’s button, garden cornflower), and there was significant development on …


The Cercospora Nicotianae Gene Encoding Dual O-Methyltransferase And Fad-Dependent Monooxygenase Domains Mediates Cercosporin Toxin Biosynthesis, Katherine L. Dekkers, Bang-Jau You, Vivek S. Gowda, Hui-Ling Liao, Miin-Huey Lee, Huey-Jiunn Bau, Peter P. Ueng, Kuang-Ren Chung Jan 2007

The Cercospora Nicotianae Gene Encoding Dual O-Methyltransferase And Fad-Dependent Monooxygenase Domains Mediates Cercosporin Toxin Biosynthesis, Katherine L. Dekkers, Bang-Jau You, Vivek S. Gowda, Hui-Ling Liao, Miin-Huey Lee, Huey-Jiunn Bau, Peter P. Ueng, Kuang-Ren Chung

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Cercosporin, a photo-activated, non-host-selective phytotoxin produced by many species of the plant pathogenic fungus Cercospora, causes peroxidation of plant cell membranes by generating reactive oxygen species and is an important virulence determinant. Here we report a new gene, CTB3 that is involved in cercosporin biosynthesis in Cercospora nicotianae. CTB3 is adjacent to a previously identified CTB1 encoding a polyketide synthase which is also required for cercosporin production. CTB3 contains a putative O-methyltransferase domain in the N-terminus and a putative flavin adenine dinucleotide (FAD)-dependent monooxygenase domain in the C-terminus. The N-terminal amino acid sequence also is similar to …


Columns Developing People Skills In The Human–Wildlife Conflict Community, Ben C. West Jan 2007

Columns Developing People Skills In The Human–Wildlife Conflict Community, Ben C. West

Human–Wildlife Interactions

No abstract provided.


Scent Deterrence To Reduce Southern Flying Squirrel Kleptoparasitism Of Red-Cockaded Woodpecker Cavities, Jonathon M. Stober, L. Mike Conner Jan 2007

Scent Deterrence To Reduce Southern Flying Squirrel Kleptoparasitism Of Red-Cockaded Woodpecker Cavities, Jonathon M. Stober, L. Mike Conner

Human–Wildlife Interactions

When establishing new populations of endangered red-cockaded woodpeckers (RCW; Picoides borealis), cavity kleptoparasites can pose a considerable obstacle to successful restoration. Southern flying squirrels (SFS; Glaucomys volans) are the principal kleptoparasite of RCW roost and nest cavities. Managers restoring RCW populations primarily use labor-intensive, direct removal to mitigate cavity competition by SFS. We fi eld tested the use of red fox (Vulpes vulpes) urine and rat snake (Elaphe spp.) musk as predator scents to examine if SFS could be deterred from using RCW cavities and to observe RCW roost behavior at cavities treated with red …


Comparison Of 2 Vegetation-Height Management Practices For Wildlife Control At Airports, Thomas W. Seamans, Scott C. Barras, Glen E. Bernhardt, Bradley F. Blackwell, Jonathon D. Cepek Jan 2007

Comparison Of 2 Vegetation-Height Management Practices For Wildlife Control At Airports, Thomas W. Seamans, Scott C. Barras, Glen E. Bernhardt, Bradley F. Blackwell, Jonathon D. Cepek

Human–Wildlife Interactions

Vegetation-height management is a potential method to reduce bird numbers at airports. Based on studies in Europe, researchers recommended vegetation heights around 25 cm; however, preliminary studies in the United States produced conflicting results regarding the effect of tall (18 to >25 cm) vegetation on bird numbers at airports. From 1999 to 2002, we compared birds and other wildlife use of 4 short-vegetation plots (mean maximum height of 15.6 cm ± 5.1 SE and visual obstruction reading of 4.6 ± 3.0 cm) and 4 tall-vegetation plots (mean maximum height of 26.9 ± 8.4 cm and visual obstruction reading of 10.0 …


The Director's Desk Hogs Gone Wild, William H. Clay Jan 2007

The Director's Desk Hogs Gone Wild, William H. Clay

Human–Wildlife Interactions

No abstract provided.


Demographic And Spatial Characteristics Of Feral Hogs In The Chihuahuan Desert, Texas, Roger N. Adkins, Louis A. Harveson Jan 2007

Demographic And Spatial Characteristics Of Feral Hogs In The Chihuahuan Desert, Texas, Roger N. Adkins, Louis A. Harveson

Human–Wildlife Interactions

Feral hogs (Sus scrofa) have recently expanded their range to include portions of the arid regions of the Chihuahuan Desert, Texas. We examined feral hog density, survival rates, range size, and habitat use in the Davis Mountains, Texas, to understand hog ecology in a desert environment. We tested the hypothesis that densities of feral hogs across Texas would be positively related to precipitation. Feral hog densities in the Chihuahuan Desert were low (0.65 individuals/km2), supporting our prediction. Annual home range sizes (100% minimum convex polygon) were also high and averaged 48.3 ± 4.4 km2 and …


Feral Hog Research In Western Louisiana: Expanding Populations And Unforeseen Consequences, Michael D. Kaller, James D. Hudson Iii, Eric C. Achberger, William E. Kelso Jan 2007

Feral Hog Research In Western Louisiana: Expanding Populations And Unforeseen Consequences, Michael D. Kaller, James D. Hudson Iii, Eric C. Achberger, William E. Kelso

Human–Wildlife Interactions

Hunter harvest data suggest that feral hog (Sus scrofa) populations in western Louisiana are increasing, and population control in this region is complicated by the mixing of feral and domestic free-ranged hogs. Aggressive management may be warranted as feral and domestic hogs appear to be having unexpected effects on their ecosystem. We present the results of 3 recent investigations of genetic source-tracking to link waterborne bacteria with bacteria from feral hogs. We integrate our most recent findings with data regarding: (1) water quality in a watershed without hog management and (2) aquatic biota of the same watershed. Hog …


Distribution And Disease Prevalence Of Feral Hogs In Missouri, R. Edwin Hartin, Mark R. Ryan, Tyler A. Campbell Jan 2007

Distribution And Disease Prevalence Of Feral Hogs In Missouri, R. Edwin Hartin, Mark R. Ryan, Tyler A. Campbell

Human–Wildlife Interactions

No attempts have been made to document the distribution of feral hogs (Sus scrofa) in Missouri. Also, antibody prevalence for pseudorabies virus and swine brucellosis have not been reported from Missouri. Our objectives were to characterize the current distribution of feral hogs in Missouri and to determine prevalence and distribution of feral hogs with antibodies against selected important diseases. We collected feral hog sighting data both from the public and Missouri Wildlife Services and Missouri Department of Conservation wildlife biologists. We determined prevalence of antibodies against pseudorabies virus, swine brucellosis, tularemia, and classical swine fever. From 2000 to …


Efficacy Of Cpth-Treated Egg Baits For Removing Ravens, Peter S. Coates, Jack O. Spencer Jr., David J. Delehanty Jan 2007

Efficacy Of Cpth-Treated Egg Baits For Removing Ravens, Peter S. Coates, Jack O. Spencer Jr., David J. Delehanty

Human–Wildlife Interactions

Human-altered landscapes have provided resource subsidies for common ravens (Corvus corax) resulting in a substantial increase in raven abundance and distribution throughout the United States and Canada in the past 25 years. Ravens are effective predators of eggs and young of ground-nesting birds. During 2002–2005, we tested whether chicken egg baits treated with CPTH (3-chloro-p-toluidine hydrochloride) could be used to manage raven numbers in an area where raven depredation was impacting sharp-tailed grouse (Tympanuchus phasianellus columbianus) and greater sage-grouse (Centrocercus urophasianus) populations in Nevada. We performed multiple raven surveys at a treatment site and …


Use Of Decoy Traps To Protect Blueberries From Juvenile European Starlings, Michael R. Conover, Richard A. Dolbeer Jan 2007

Use Of Decoy Traps To Protect Blueberries From Juvenile European Starlings, Michael R. Conover, Richard A. Dolbeer

Human–Wildlife Interactions

Fruit consumption by large flocks of juvenile European starlings (Sturnus vulgaris) is a serious problem for growers of strawberries, grapes, apples, cherries, blueberries, and other small fruit. This study examined if numbers of juvenile European starlings foraging in blueberry orchards could be reduced by catching them in decoy traps and relocating the birds elsewhere. From late July through August of 1989, 620 juvenile starlings were captured in 2 decoy traps at a blueberry orchard in Connecticut. A similar number were caught during the same period in 1990. During these 2 years, numbers of juvenile starlings foraging daily in …


Trampling, Peeling And Nibbling Mussels: An Experimental Assessment Of Mechanical And Predatory Damage To Shells Of Mytilus Trossulus (Mollusca: Mytilidae), Carlos E. Cintra-Buenrostro Jan 2007

Trampling, Peeling And Nibbling Mussels: An Experimental Assessment Of Mechanical And Predatory Damage To Shells Of Mytilus Trossulus (Mollusca: Mytilidae), Carlos E. Cintra-Buenrostro

School of Earth, Environmental, & Marine Sciences Faculty Publications

Shell damage, if properly recognized, can provide information about biotic interactions between molluscs and their predators. However, it can be difficult to distinguish predatory damage from mechanical breakage, thus making interpretation of damaged modern and fossil shells problematic. To establish a clear-cut distinction between antemortem predatory crab damage and ante- and postmortem mechanical damage in Mytilus trossulus shells, a combined field and experimental approach was used. Mussels were exposed to predation by crabs, tumbled-live, tumbled-dead, and trampled. After 100 h of tumbling, live-collected mussel shells were abraded and disarticulated but not otherwise damaged. Eight percent of the dead-collected shells were …


Role Of Ceramide-1-Phosphate As A Specific And Potent Activator Of Group Iva Cytosolic Phospholipase A2 Alpha, Preeti Subramanian Jan 2007

Role Of Ceramide-1-Phosphate As A Specific And Potent Activator Of Group Iva Cytosolic Phospholipase A2 Alpha, Preeti Subramanian

Theses and Dissertations

Eicosanoids are potent mediators of inflammatory response whose role has been well established in inflammatory disorders. Release of arachidonic acid by group IVA cytosolic phospholipase A2 α (cPLA2α) is the initial rate limiting step for the production of eicosonoids in response to inflammatory mediators. Previous findings from our laboratory have demonstrated that cPLA2α is directly activated by the emerging bioactive sphingolipid, ceramide-1-phosphate (C1P). In this study, we have developed a modified Triton X-100/phosphatidylcholine (PC) mixed micelle assay which was utilized to determine the kinetics and specificity of this lipid-enzyme interaction. Using this assay, the activity of the enzyme increased in …


Cell Signaling Pathways In Lps-Mediated Apoptosis Of Dedifferentiated Hepatoma Cells, Michael Ryan Reidy Jan 2007

Cell Signaling Pathways In Lps-Mediated Apoptosis Of Dedifferentiated Hepatoma Cells, Michael Ryan Reidy

Masters Theses

Using liver tumor cells and their dedifferentiated cell lines, mechanisms underlying tissue specific gene expression can be studied. We have derived cell lines, from liver tumor cells, which fail to express liver genes. These cells are termed "dedifferentiated cells". One significant observation made was that cells which fail to express liver genes also rapidly undergo programmed cell death (known as apoptosis) under the influence of the bacterial endotoxin lipopolysaccharide (LPS). Understanding the mechanism of how liver function relates to cell apoptosis is the goal of this study.

Apoptosis is known to play a critical role in development and homeostasis, yet …


Comparison Of Avian Nest Success Among Linear Wooded Habitats In An Agricultural Landscape, Terri J.E. Thompson Jan 2007

Comparison Of Avian Nest Success Among Linear Wooded Habitats In An Agricultural Landscape, Terri J.E. Thompson

Masters Theses

In the Midwest, urban expansion and more intensive agricultural practices have reduced habitat available to most species of breeding birds. Linear wooded habitats including fencerows and riparian strips are a common habitat feature in many agricultural landscapes. Changes in agricultural practices and the subsequent reduction in grasslands and fencerows have lead to greater breeding densities in species that use the remaining fencerows as nest sites. This may lead to a lower nesting success due to factors such as higher predation risk. Specifically, it is not known how such linear habitats currently contribute to avian populations in central Illinois.

We monitored …


Effects Of Food Deprivation, Body Size, And Egg Load On The Mating Behavior Of Female Japanese Beetles, Popillia Japonica Newman (Coleoptera: Scarabaeidae), Natasha Tigreros Jan 2007

Effects Of Food Deprivation, Body Size, And Egg Load On The Mating Behavior Of Female Japanese Beetles, Popillia Japonica Newman (Coleoptera: Scarabaeidae), Natasha Tigreros

Masters Theses

Female condition may play an important role in the mating frequency of species that mate at foraging areas. For example, in species where feeding females are more easily encountered or less resistant to mating than females that are performing other activities, low energy levels could increase mating activity. We studied the relationship between female condition and mating activity in the Japanese beetle, Popillia japonica (Coleoptera: Scarabaeidae) in a laboratory study. We altered condition by depriving some females of food for one day and comparing their mating behavior to well-fed females. Our results showed that the effect of condition on mating …


Red Squirrel Distribution And Habitat Use In Illinois, Corinna Hanson Jan 2007

Red Squirrel Distribution And Habitat Use In Illinois, Corinna Hanson

Masters Theses

The North American red squirrel (Tamiasciurus hudsonicus) is a species of special concern in Illinois due to its restricted geographic range and low numbers. This species occupies a broad geographic range in North America and is usually associated with the boreal forests of Canada and coniferous forests in the Rocky Mountain range. The squirrels also occupy deciduous and mixed deciduous-coniferous forests in the eastern U.S. In Illinois, available habitat is suboptimal, and limited to pine plantations and small tracts of deciduous forest embedded in an agricultural matrix landscapе. Information on the spatial distribution of red squirrels in northeastern Illinois was …


Mating, Kinship, And Population Structure In Illinois Beaver Populations, Joanne C. Crawford Jan 2007

Mating, Kinship, And Population Structure In Illinois Beaver Populations, Joanne C. Crawford

Masters Theses

The North American beaver, Castor canadensis, is an important wildlife species for a variety of reasons. As a furbearer, it provides income and recreation to trappers throughout the state. As a wetland engineer, it helps maintain functioning ecosystems (Naiman et al. 1986, McKinstry and Anderson 2002). Finally, the beaver serves as a model organism for studies of mating and kinship, as it has beed reported to be almost exclusively monogamous (Sun 2003).

Monogamy in mammals is generally restricted to the primates, canids, and rodents, and is defined as a mating system in which a pair remains together for at least …


Classification Of Illinois Lakes And Reservoirs And Evaluation Of The Potential Use Of Phytoplankton As Biocriteria, Jessica A. Rasmussen Jan 2007

Classification Of Illinois Lakes And Reservoirs And Evaluation Of The Potential Use Of Phytoplankton As Biocriteria, Jessica A. Rasmussen

Masters Theses

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Habitat Use And Overwintering Ecology Of The Northern Watersnake (Nerodia Sipedon) On An Artificial Levee In Central Illinois, Melanie J. Olds Jan 2007

Habitat Use And Overwintering Ecology Of The Northern Watersnake (Nerodia Sipedon) On An Artificial Levee In Central Illinois, Melanie J. Olds

Masters Theses

Several reptile species have populations that are in decline, and anthropogenic habitat loss and alteration are arguably the factors having the greatest impact on these species. More attention has been paid to understanding the activity season habitat needs in snakes than on the habitat requirements for overwintering. I examined the patterns of both activity season and overwintering habitat use by a population of northern watersnakes (Nerodia sipedon) that inhabit an artificial levee in central Illinois. My research objectives were to determine how a population of northern watersnakes uses an artificial levee during the activity season and to determine what type …


Influence Of Fish Presence And Removal On Woodland Pond Breeding Amphibians, J. Brian Towey Jan 2007

Influence Of Fish Presence And Removal On Woodland Pond Breeding Amphibians, J. Brian Towey

Masters Theses

Predatory fish can have devastating effects on populations of amphibians, many of which are already declining around the world. Land managers seeking to restore disturbed woodland ponds to more historically-accurate conditions may consider removing fish, a potentially difficult or costly process. I report on the effects of introduced sunfish (Lepomis spp.) and black bullhead catfish (Ameiurus melas), and their removal, on the reproductive efforts of three species of amphibians: smallmouth salamanders (Ambystoma texanum), American toads (Bufo americanus) and wood frogs (Rana sylvatica). Using drift fences and pitfall traps around four woodland ponds, amphibians were monitored for one year prior to …


Law, Responsibility, And The Brain, Owen D. Jones, Hakwan C. Lau, Dean Mobbs, Christopher D. Frith Jan 2007

Law, Responsibility, And The Brain, Owen D. Jones, Hakwan C. Lau, Dean Mobbs, Christopher D. Frith

Vanderbilt Law School Faculty Publications

This article addresses new developments in neuroscience, and their implications for law. It explores, for example, the relationships between brain injury and violence, as well as the connections between mental disorders and criminal behaviors. It discusses a variety of issues surrounding brain fingerprinting, the use of brain scans for lie detection, and concerns about free will. It considers the possible uses for, and legal implications of, brain-imaging technology. And it also identifies six essential limits on the use of brain imaging in courtroom procedures.


Endowment Effects In Chimpanzees, Owen D. Jones, Sarah F. Brosnan, Susan P. Lambeth, Mary Catherine Mareno, Amanda S. Richardson, Steven Schapiro Jan 2007

Endowment Effects In Chimpanzees, Owen D. Jones, Sarah F. Brosnan, Susan P. Lambeth, Mary Catherine Mareno, Amanda S. Richardson, Steven Schapiro

Vanderbilt Law School Faculty Publications

Human behavior is not always consistent with standard rational choice predictions. The much-investigated variety of apparent deviations from rational choice predictions provides a promising arena for the merger of economics and biology. Although little is known about the extent to which other species also exhibit these seemingly irrational patterns of human decision-making and choice behavior, similarities across species would suggest a common evolutionary root to the phenomena.

The present study investigated whether chimpanzees exhibit an endowment effect, a seemingly paradoxical behavior in which humans tend to value a good they have just come to possess more than they would have …


Law, Responsibility, And The Brain, Owen D. Jones, Dean Mobbs, Hakwan C. Lau, Christopher D. Frith Jan 2007

Law, Responsibility, And The Brain, Owen D. Jones, Dean Mobbs, Hakwan C. Lau, Christopher D. Frith

Vanderbilt Law School Faculty Publications

This article addresses new developments in neuroscience, and their implications for law. It explores, for example, the relationships between brain injury and violence, as well as the connections between mental disorders and criminal behaviors. It discusses a variety of issues surrounding brain fingerprinting, the use of brain scans for lie detection, and concerns about free will. It considers the possible uses for, and legal implications of, brain-imaging technology. And it also identifies six essential limits on the use of brain imaging in courtroom procedures.


Giant Pandas Balance Energy Budgets With Metabolic Depression: Insights From Radar Detection Of Heart Rates, Tommy Gene Finley Jan 2007

Giant Pandas Balance Energy Budgets With Metabolic Depression: Insights From Radar Detection Of Heart Rates, Tommy Gene Finley

Theses and Dissertations

Giant pandas ( Ailuropoda melanoleuca ) are flagship species for conservation because of their endangered status and charismatic nature. Giant pandas are placed in the bear family (Urisidae) within the order Carnivora. They specialize on a diet of bamboo, yet retain a typical carnivore gastrointestinal tract. Low energy digestive efficiency (9.5–34%) was calculated from bamboo-only digestibility trials conducted on captive giant pandas. Huge amounts of bamboo are consumed to overcome inefficient extraction and thus obtain sufficient energy and nutrients. I designed this study to gain insights into giant panda energy balance by measuring heart rates during sleep, their primary means …


Establishment And Maintenance Of An Asm Student Chapter, Amy Cheng Vollmer Jan 2007

Establishment And Maintenance Of An Asm Student Chapter, Amy Cheng Vollmer

Biology Faculty Works

No abstract provided.


Stabel Isotope Turnover Rates And Diet-Tissue Discrimination In The Skin Of West Indian Manatees: Implcations For Evaluating Their Feeding Ecology And Habitat Use, Christy Alves Jan 2007

Stabel Isotope Turnover Rates And Diet-Tissue Discrimination In The Skin Of West Indian Manatees: Implcations For Evaluating Their Feeding Ecology And Habitat Use, Christy Alves

Electronic Theses and Dissertations

The West Indian manatee (Trichechus manatus) is an herbivorous marine mammal that occupies freshwater, estuarine, and marine habitats. Despite being considered endangered, relatively little is known about the feeding ecology of either of the two recognized subspecies, the Florida manatee (T.m. latirostris) and Caribbean or Antillean manatee (T.m. manatus). A better understanding of their respective feeding preferences and habitat use is essential to establish criteria on which conservation plans can be based. The present study expands on previous work on manatee feeding ecology by both assessing the application of stable isotope analysis to manatee tissue and providing critical baseline parameters …