Life Sciences Commons^™

Computer-Assisted Docking Of Flavodoxin With The Atp:Co(I)Rrinoid Adenosyltransferase (Coba) Enzyme Reveals Residues Critical For Protein-Protein Interactions But Not For Catalysis*, Nicole R. Buan, Jorge C. Escalante-Semerena

Department of Biochemistry: Faculty Publications

The activity of the housekeeping ATP:co(I)rrinoid adenosyltransferase (CobA) enzyme of Salmonella enterica sv. Typhimurium is required to adenosylate de novo biosynthetic intermediates of adenosylcobalamin and to salvage incomplete and complete corrinoids from the environment of this bacterium. In vitro, reduced flavodoxin (FldA) provides an electron to generate the co(I)rrinoid substrate in the CobA active site. To understand how CobAand FldA interact, a computer model of aCobA∙FldA complex was generated. This model was used to guide the introduction of mutations into CobA using site-directed mutagenesis and the synthesis of a peptide mimic of FldA. Residues Arg-9 and Arg-165 of CobA …

Small Grains And Field Peas: 2006 Variety Recommendations (2005 Crop Performance Results), Cooperative Extension Service, South Dakota State University

SDSU Extension Circulars

Variety selection is a fundamental element in a sound crop production program. This report contains variety recommendations, descriptions, and yield data for the spring-seeded small grains – hard red spring wheat, oat, and barley, along with the fall-seeded small grain –hard red winter wheat. Key factors in variety selection include yield, yield stability, maturity, straw strength, height, test weight, quality, and disease resistance. Yield is an important factor; however, a variety with good disease resistance, straw strength, and high grain quality may be more profitable in some cases than the highest yielding variety. Disease resistance information is based on reactions …

Exposure To Methamphetamine And Phencyclidine During Development And Subsequent Behavioral Change, Takehiro Minamoto

Morehead State Theses and Dissertations

A thesis presented to the faculty of the College of Science and Technology at Morehead State University in partial fulfillment of the requirements for the Degree of Master of Science by Takehiro Minamoto on December 2, 2005.

Establishment Stand Thresholds For Switchgrass Grown As A Bioenergy Crop, Marty R. Schmer, Kenneth P. Vogel, Robert B. Mitchell, Lowell E. Moser, Kent M. Eskridge, Richard K. Perrin

Department of Agronomy and Horticulture: Faculty Publications

Switchgrass (Panicum virgatum L.) is a warm-season (C4) perennial grass and a potential bioenergy crop. On-farm switchgrass field scale trials, which were initiated to obtain economic production information for switchgrass grown as a bioenergy crop in the northern Plains, provided information on establishment year stands and post-establishment year yields and stands both within and across fields and were used to determine if a stand threshold exists for switchgrass grown as a biomass energy crop. Switchgrass was seeded in 10 cropland fields, ranging in size from 3 to 9.5 ha, in Nebraska, South Dakota, and North Dakota in 2000 and …

Western Rock Lobster Fishery, W J. Fletcher, C Chubb, J Mccrea, N Caputi, F J. Webster, Ross Gould, Tim Bray

Ecologically Sustainable Development (ESD) series

Ecologically Sustainable Development (ESD) is the concept that seeks to integrate short and longterm economic, social and environmental effects in all decision-making. The Western Australian Government is committed to the concepts of ESD and these principles are implicitly contained in the objectives of the Fisheries Resources Management Act 1994 (FRMA).

Openws-Transaction: Enabling Reliable Web Service Transactions, Ivan Vasquez, John A. Miller, Kunal Verma, Amit P. Sheth

Kno.e.sis Publications

OpenWS-Transaction is an open source middleware that enables Web services to participate in a distributed transaction as prescribed by the WS-Coordination and WS-Transaction set of specifications. Central to the framework are the Coordinator and Participant entities, which can be integrated into existing services by introducing minimal changes to application code. OpenWS-Transaction allows transaction members to recover their original state in case of operational failure by leveraging techniques in logical logging and recovery at the application level. Depending on transaction style, system recovery may involve restoring key application variables and replaying uncommitted database activity. Transactions are assumed to be defined in …

Limits Of Temperature Tolerance Of Leishmania Enriettii And Leishmania Hertigi In A Real-Time Pcr Assay, Christina Salinas

Theses & Dissertations

Protozoans of the genus Leishmania are intracellular parasites found across five continents. They cycle between a promastigote form inside the digestive tracts of blood-feeding female sand flies and an amastigote form within mammalian host macrophages. Temperature is an important factor that must be defeated in order for the parasites' survival and continuance. This paper studies the limits of temperature tolerance by Leishmania enriettii and Leishmania hertigi. It was hypothesized that a correlation would exist between the limits of temperature tolerance to body temperature of their host. I explored growth characteristics at 21,27,29, 33, 35, 37, and 39° C. It was …

Monthly Variability In Florida Bay Benthic Foraminifera Community Structure, C. Featherstone, Patricia Blackwelder

Marine & Environmental Sciences Faculty Proceedings, Presentations, Speeches, Lectures

Florida Bay is a shallow subtropical estuary, which experiences highly variable environmental fluctuations due to natural forces (hurricanes, climatic variations and sea level rise) and anthropogenic influences (agricultural activity, water management and urbanization). Study of short time-scale variability in benthic community population structure and synchronous environmental change is essential to understanding forcing relationships between environment and its effects on population. Benthic foraminifera assemblage variability is an excellent indicator of environmental change in estuarine and coastal areas because populations may respond relatively quickly on spatial and temporal scales (Alve, 1995). Temporal resolution is enhanced because foraminifera may reproduce as often as …

Stemborers Associated With Smooth Cordgrass, Spartina Alterniflora (Poaceae), In A Nursery Habitat, W. H. White, D. Adamski, G. Fine, E. P. Richard, Jr.

USDA Systematic Entomology Laboratory

Extensive ecological studies have been conducted on insects inhabiting native stands of smooth cordgrass, Spartina alterniflora Loisel; however, this is not the case for insects found in smooth cordgrass in a nursery habitat. We investigated species composition and larval disposition among stemborers (Lepidoptera: Coleophoridae and Crambidae) infesting stems of smooth cordgrass in nursery plots. One thousand and forty stems of smooth cordgrass were randomly selected in 2003 and examined for presence of stemborer larvae. Height of larvae on or within stem, height of stem from ground level to top-visible dewlap, and condition of the leaf-whorl were documented. Stemborers representing six …

The Role Of An Explicit Causal Framework In Affected Sib Pair Designs With Covariates , Constantine E. Frangakis, Fan Li, Betty Q. Doan

Johns Hopkins University, Dept. of Biostatistics Working Papers

The affected sib/relative pair (ASP/ARP) design is often used with covariates to find genes that can cause a disease in pathways other than through those covariates. However, such "covariates" can themselves have genetic determinants, and the validity of existing methods has so far only been argued under implicit assumptions. We propose an explicit causal formulation of the problem using potential outcomes and principal stratification. The general role of this formulation is to identify and separate the meaning of the different assumptions that can provide valid causal inference in linkage analysis. This separation helps to (a) develop better methods under explicit …

Center For Grassland Studies Newsletter, Winter 2005, Volume 11, No. 1

Center for Grassland Studies: Newsletters

Grass Seed Production Research in Nebraska Panhandle by David Baltensperger, Department of Agronomy and Horticulture, UNL

Can Perennial Grass Pastures be Profitable in the Great Plains by Rob Mitchell, Ken Vogel, and Gary Varvel, USDA-ARS, Lincoln, NE Terry Klopfenstein (Animal Science), Dick Clark (Agricultural Economics), and Bruce Anderson (Agronomy and Horticulture), UNL

Forum – Monitoring Rangeland Health for an Experiment in Public Land Ownership

2005 Nebraska Grazing Conference to be Aug. 8-9

PGM Program Swings into 2nd Semester

Dates for 7th Nebraska Ranch Practicum Set

Changing Natural Landscapes: Ecological and Human Dimensions

Farm Service Agency Direct Farm Loan Program Effectiveness Study, John Nwoha, Bruce L. Ahrendsen, Bruce L. Dixon, Eddie C. Chavez, Sandra J. Hamm, Daniel M. Settlage, Diana Danforth

AAES Research Reports and Research Bulletins

This final report presents the results of an independent, performance-focused review of the effectiveness of Farm Service Agency (FSA) Direct Farm Loan Programs (FLPs) as requested by the Office of Management and Budget (OMB) in the 2005 Passback for FSA. The study focuses on FSA’s direct Farm Ownership (FO), Farm Operating (OL), and Emergency (EM) loan programs.

Demonstrating Dynamic Configuration And Execution Of Web Processes, Karthik Gomadam, Kunal Verma, Amit P. Sheth, John A. Miller

Kno.e.sis Publications

Web processes are next generation workflows on the web, created using Web services. In this paper we demonstrate the METEOR-S Configuration and Execution Environment (MCEE) system. It will illustrate the capabilities of the system to a) Discover partners b) Optimize partner selection using constraint analysis, c) Perform interaction protocol and data mediation. A graphical execution monitor to monitor the various phases of execution will be used to demonstrate various aspects of the system.

2004 Coastal Municipal Stormwater Infrastructure Mapping Project, Jeffrey Marcoux

PREP Reports & Publications

The New Hampshire Department of Environmental Services (DES) received funds in 2004 from the New Hampshire Estuaries Project (NHEP) to provide assistance to coastal communities to develop storm sewer infrastructure maps. This final report describes the grant project that was completed in Rochester. NHEP chose to fund stormwater infrastructure mapping projects for a number of reasons. Primarily, this grant was established in order to fulfill one of the water quality action plans identified in the NHEP Management Plan. In addition, the Coastal/Piscataqua watershed has been identified by DES as a priority watershed in need of restoration. Updated and comprehensive maps …

Summary Of Coastal And Estuarine Monitoring Programs In New Hampshire (2005), Phil Trowbridge

PREP Reports & Publications

The New Hampshire Estuaries Project (NHEP) compiles data from many coastal and estuarine monitoring programs to assess the status and trends of environmental indicators in the Great Bay and Hampton/Seabrook Harbor. The full list of NHEP environmental indicators has been published in the NHEP Monitoring Plan. The following catalog is a summary of the coastal and estuarine monitoring programs that provide data for NHEP indicators or for State water quality assessments. This list is limited to long-term monitoring programs that do not have an end date.

Select Listeria Monocytogenes Subtypes Commonly Found In Foods Carry Distinct Nonsense Mutations In Inla, Leading To Expression Of Truncated And Secreted Internalin A, And Are Associated With A Reduced Invasion Phenotype For Human Intestinal Epithelial Cells, K. K. Nightingale, K. Windham, K. E. Martin, M. Yeung, M. Wiedmann

Biological Sciences

The surface protein internalin A (InlA) contributes to the invasion of human intestinal epithelial cells by Listeria monocytogenes. Screening of L. monocytogenes strains isolated from human clinical cases (n=46), foods (n=118), and healthy animals (n=58) in the United States revealed mutations in inlA leading to premature stop codons (PMSCs) in L. monocytogenes ribotypes DUP-1052A and DUP-16635A (PMSC mutation type 1), DUP-1025A and DUP-1031A (PMSC mutation type 2), and DUP-1046B and DUP-1062A (PMSC mutation type 3). While all DUP-1046B, DUP-1062A, DUP-16635A, and DUP-1031A isolates (n=76) contained inlA PMSCs, ribotypes DUP-1052A and DUP-1025A ( …

[Accepted Article Manuscript Version (Postprint)] Identification And Functional Characterization Of Arabidopsis Peroxin4 And The Interacting Protein Peroxin22, Bethany Zolman, Melanie Monroe-Augustus, Illeana Silva, Bonnie Bartel

Biology Department Faculty Works

Peroxins are genetically defined as proteins necessary for peroxisome biogenesis. By screening for reduced response to indole-3-butyric acid, which is metabolized to active auxin in peroxisomes, we isolated an Arabidopsis thaliana peroxin4 (pex4) mutant. This mutant displays sucrose-dependent seedling development and reduced lateral root production, characteristics of plant peroxisome malfunction. We used yeast two-hybrid analysis to determine that PEX4, an apparent ubiquitinconjugating enzyme, interacts with a previously unidentified Arabidopsis protein, PEX22. A pex4 pex22 double mutant enhanced pex4 defects, confirming that PEX22 is a peroxin. Expression of both Arabidopsis genes together complemented yeast pex4 or pex22 mutant defects, whereas expression …

Fisheries Research Report No. 152 - Development Of A Dna Database For Compliance And Management Of Western Australian Sharks., Rory Mcauley, K. Ho, R. Thomas

Fisheries Research Reports

Final FRDC Report - Project 2003/067

The capability to identify individual shark species from processed body parts is necessary for the WA Department of Fisheries to ensure the compliance of all WA fisheries with both existing protected species regulations and proposed new management measures for commercially important shark species. This project established a reference database of genetic profiles or ‘fingerprints’ for nine of Western Australia’s protected and commercially important shark species. Fingerprints from a 10th species, the shortfin mako (Isurus oxyrinchus) could not be obtained due to probable primer sequence mismatching. The database, in conjunction with an associated inspection and …

The Perceptions Of Roles And Practices Of Preceptors And Their Expectations For Students In The Coordinated Program In Dietetics At Indiana State University, Carin R. Aloisio

All-Inclusive List of Electronic Theses and Dissertations

Dietetics education requires a minimum of a baccalaureate degree and 900 hours of supervised practice for eligibility of candidates to sit for the Registration Exam. The primary goals of this study are to determine perceptions of preceptors of supervised practice about their roles and practices and their expectations for students, and to compare and/or contrast these between the three main areas of dietetics: clinical, community and food service management.

The Effects Of Heat Stress On High Oil Corn, Summer Goldman

Masters Theses & Specialist Projects

High oil corn (HOC) is essentially dent corn that has been selected for high oil content in the scutellum. It is a value-added crop that can potentially offer a premium price for producers. Though southeastern U.S. producers have not had problems achieving sufficient yields, the oil content necessary for premium prices has been elusive. One of the most evident differences between these growers and their northern counterparts is the climate. Temperatures during the reproductive growth and grain fill periods are higher in southern latitudes. A two-part research project was conducted to further investigate the significance of temperature on oil content. …

Bartonella Bacilliformis Groel: Effect On Growth Of Human Vascular Endothelial Cells In Infected Cocultures, Laura S. Smitherman, Michael F. Minnick

Biological Sciences Faculty Publications

Bartonella are the only bacteria known to induce angioproliferative lesions of the human vasculature and liver during infection. Previous work from our lab suggests that GroEL participates in the mitogenic response observed in HUVEC cultures supplemented with the soluble fraction of Bartonella bacilliformis. Work in this study shows that exposure to high concentrations of the fraction is actually cytotoxic for HUVECs. To analyze this phenomenon, live B. bacilliformis-HUVEC cocultures were employed to study the effect of excess bacterial GroEL on the host cell during active infection. Four B. bacilliformis strains were generated to produce varying levels of GroEL. HUVEC cocultures …

2004 Coastal Illicit Connection Identification And Elimination Grant Project, Jeffrey Marcoux

PREP Reports & Publications

The New Hampshire Department of Environmental Services (DES) received funds in 2004 from the New Hampshire Estuaries Project (NHEP) to administer grants to coastal municipalities to eliminate illicit discharges into their storm drainage systems. This final report describes the two projects that were funded under this grant. Projects in Dover, and Portsmouth involved eliminating sewage discharges into storm drainage systems from houses, apartment buildings, and commercial buildings. All of these efforts helped improve water quality in the coastal area by reducing pollution. NHEP chose to fund illicit discharge detection and elimination projects for a number of reasons. Primarily, this grant …

Spatial Autocorrelation Of Deer-Vehicle Accidents In Vigo County, J. Scott Horn

All-Inclusive List of Electronic Theses and Dissertations

Deer-vehicle accidents (DV As) are a problem in many areas. They cause millions of dollars in property damage, personal injury, and loss of life each year. This thesis examines whether DVAs in Vigo County, Indiana during the year 2000 were spatially autocorrelated; and whether DV As selected by deer habitat were spatially autocorrelated. Specifically using Moran's I to test for spatial autocorrelation this study determined if and to what degree spatial autocorrelation existed among these DVAs. A Moran's I value of .0023 shows that the accidents occurred randomly. These DV A data were then used to plot a map which …

Biomass Estimation Using Statistical And Neural Network Analysis Of Aster Data, Vijay O. Lulla

All-Inclusive List of Electronic Theses and Dissertations

This study assessed the performance of different biomass estimation methods using Advanced Spaceborne Thermal Emission and Reflection Radiometer (ASTER) lll data in a temperate forest. Multiple linear regression statistics of spectral band data and derived indices with biomass values were compared with advanced neural network models created with spectral band data and indices to model biomass values. Biomass for the study area was estimated using both of these methods and the results were discussed. The data were analyzed using multivariate statistical analysis. Correlation analysis and regression analysis were employed to understand the relationship of biomass to the spectral data and …

Fisheries Research Report No. 151 - Biology And Stock Assessment Of The Thickskin (Sandbar) Shark, Carcharhinus Plumbeus, In Western Australia And Further Refinement Of The Dusky Shark, Carcharhinus Obscurus, Stock Assessment., Rory Mcauley, R Lenanton, J. Chidlow, R. Allison, E. Heist

Fisheries Research Reports

Final FRDC Report - Project 2000/134

The purpose of this project was to collect the biological and fishery information necessary to conduct a stock assessment of the sandbar (known locally as ‘thickskin’) shark, Carcharhinus plumbeus and to improve and update the existing stock assessment for the dusky shark, Carcharhinus obscurus. Results from this project have already been used by the Western Australian Department of Fisheries to determine appropriate management arrangements for the State’s shark fisheries to ensure the sustainable exploitation of these species. Results have also assisted the WA target-shark fisheries in conducting Ecologically Sustainable Development (ESD) assessment in order …

Aquatic Animal Health Subprogram: Technical Guidelines For The Translocation Of Live Aquatic Animals - With Reference To Barramundi Nodavirus, Brian Jones

Fisheries Research Reports

FRDC Project No. 2005/640

A workshop was conducted involving input from most jurisdictions. Technical issues including hazard identification and risk management associated with the translocation of live finfish were documented.

A series of recommendations, which resulted from the workshop process, will be progressed through the National Aquatic Animal Health Technical Working group and the Aquatic Animal Health Committee.

Ard News December 2005

Agricultural Research Division: News and Annual Reports

CONTENTS:
Comments from the Interim Dean
William G. Whitmore Student Travel Endowment
David H. and Annie E. Larrick Fund, 2006
Undergraduate Honors Research Program
New or Revised Projects September and October 2005
Proposals Submitted for Federal Grants September and October 2005
Grants and Contracts Received for September and October 2005
Fall 2005 Graduate Census

Bayer Ag V. Housey Pharmaceuticals: Protection For Biotechnological Research Tools Under Section 271(G) Found Wanting, Matthew Barthalow

The University of New Hampshire Law Review

[Excerpt] "Research tools, a subset of biotechnological inventions protected by process patents, are “tools that scientists use in the laboratory, including cell lines, monoclonal antibodies, reagents, animal models, growth factors, combinatorial chemistry and DNA libraries, clones and cloning tools (such as PCR), methods, laboratory equipment and machines.” Many companies base their business models on the ability to find pharmaceutical products using their proprietary drug discovery research tools. Research tools used for drug discovery ‘include bioinformatic methods for identifying the interaction of certain proteins and their association with disease, methods for confirming protein targets, screening assays to identify molecules active against …

The Eggshell Structures Of Ringed Turtle - Doves (Streptopelia Risoria), Natalie Ellen Hubbard

Biology Theses - Archive

The avian egg provides nutrients and protects the growing embryo from physical and microbial harm. Structures fundamental to the protection are the membrane layers, which are tightly meshed networks of collagen fibers, and the eggshell, which is composed of calcite crystals, (CaC03). The eggs of Ringed Turtle-Dove and commercially bred chickens were selected for the study. Eggs of commercially bred chickens are twice in length and width of the dove eggs. Features analyzed are components of quality and strength of eggshells included shell thickness, membrane thickness, and concentration and size of vesicles. A comparative study of the bird eggs was …

Reproductive Behaviors Of Two Congeneric, Sympatric Jumping Spiders: Gene Flow Barriers And Polyandry, Stacey Nicole Gingras

Biology Theses - Archive

This two part study compares aspects of jumping spider reproductive behavior and their implications 1) as gene flow barriers during the process of speciation and 2) in the occurrence of polyandry. Courtship behaviors of Phidippus audax and P. carolinensis were observed. P. carolinensis displays simple courtship behavior when compared to the visually complex behavior of P. audax. P. carolinensis males appear to be incorporating a vibratory element into their courtship display, and a possible stridulatory organ, located on the male pedipalp, is described. Large differences in courtship display, complexity of courtship badges, and phenologies between the species most likely prevent …