Life Sciences Commons^™

Tsc2 Modulates Actin Cytoskeleton And Focal Adhesion Through Tsc1-Binding Domain And The Rac1 Gtpase, Elena Goncharova, Dmitry Goncharov, Daniel J. Noonan, Vera P Krymskaya

Molecular and Cellular Biochemistry Faculty Publications

Tuberous sclerosis complex (TSC) 1 and TSC2 are thought to be involved in protein translational regulation and cell growth, and loss of their function is a cause of TSC and lymphangioleiomyomatosis (LAM). However, TSC1 also activates Rho and regulates cell adhesion. We found that TSC2 modulates actin dynamics and cell adhesion and the TSC1-binding domain (TSC2-HBD) is essential for this function of TSC2. Expression of TSC2 or TSC2-HBD in TSC2-/- cells promoted Rac1 activation, inhibition of Rho, stress fiber disassembly, and focal adhesion remodeling. The down-regulation of TSC1 with TSC1 siRNA in TSC2-/- cells activated Rac1 and induced loss of …

Conservation Easement Outreach Project, Wenninger, Ray Wenninger

PREP Reports & Publications

Stratham voters approved a 5 million dollar conservation bond issue in 2002 to preserve open space through easement or outright purchase. The Ad-hoc Conservation Bond Subcommittee to the Conservation Commission is helping achieve Stratham’s open space goals and is continuing to reach out to landowners that have undeveloped land that may be suitable for Stratham’s open space initiative. It was an opportune time for the subcommittee to provide updated outreach materials when a grant by the Natural Resources Outreach Coalition (NROC) Implementation Grant Program became available. The grant, managed by the New Hampshire Estuaries Project, provided funding to produce and …

A Comparison Of Linear And Daily Undulating Periodizied Strength Training Programs., Andrew Morrow Caldwell

Electronic Theses and Dissertations

The purpose of this study is to compare linear periodized (LP) and daily undulating (DUP) periodized strength training programs, and determine if either method of periodization elicits superior gains in 1 Repetitions maximums (1RM) for back squat (BS) and bench press (BP) (core exercises). Nineteen subjects (n=19) underwent a 6-week resistance-training (RT) program. Participants performed both BS and BP two day a week. The LP group linearly increased intensity while decreasing volume. While the DUP group daily changed intensity and volume. Total volume for both BS and BP were equal for both LP and DUP. Both BP and BS increased …

Registered Dietitans Practicing Advanced Level Skills In The State Of Tennessee And Their Perceived Job Satisfaction., Charlotte Norene Cochran

Electronic Theses and Dissertations

The purpose of this study was to ascertain the number of registered dietitians in Tennessee who perceive they are practicing at advanced levels versus those making recommendations only. Job satisfaction according to order writing privileges was also assessed. A five question survey was sent to hospitals meeting selection criteria. Thirty-three surveys (89%) were returned. Eighty-nine percent of dietitians with order writing privileges considered themselves to be advanced level practitioners compared to 60% in the group of dietitians who did not have order writing privileges. Dietitians with order writing privileges indicated greater job satisfaction compared to dietitians that did not have …

Isolation Of Cancer Stem Cells From Adult Glioblastoma Multiforme, Xianpeng Yuan, James Curtin, Yizhi Xiong, Gentao Liu, Sebastian Waschsmann-Hogiu, Daniel Farkas, Keith Black, John Yu

Articles

Glioblastoma multiforme (GBM) is the most common adult primary brain tumor and is comprised of a heterogeneous population of cells. It is unclear which cells within the tumor mass are responsible for tumor initiation and maintenance. In this study, we report that brain tumor stem cells can be identified from adult GBMs. These tumor stem cells form neurospheres, possess the capacity for self-renewal, express genes associated with neural stem cells (NSCs), generate daughter cells of different phenotypes from one mother cell, and differentiate into the phenotypically diverse populations of cells similar to those present in the initial GBM. Having a …

Effect Of Stocking Density On Sunshine Bass (Morone Chrysops X M. Saxatilis) Production And Water Quality At Minor Clark Fish Hatchery, Kentucky, Todd A. Leonard

Morehead State Theses and Dissertations

A thesis presented to the faculty of the College of Science and Technology at Morehead State University in partial fulfillment of the Requirements for the Degree of Master of Science by Todd A. Leonard on December 15, 2004.

West River Ag Center Crops And Soils Research Annual Progress Report, 2004, Agricultural Experiment Station

Agricultural Experiment Station and Research Farm Annual Reports

This is the 2004 progress report of the West River Crops and Soils Research Projects, South Dakota Agricultural Experiment Station. This document includes reports on: weather and climate, wheat and grain variety trials, management and tillage, and weed and pest control.

Expression Of The Tobacco Β- 1,3-Glucanase Gene, Pr-2d, Following Induction Of Sar With Peronospora Tabacina, Deanna L. Funnell, Christopher B. Lawrence, Jeffrey F. Pedersen, Christopher L. Schardl

Department of Plant Pathology: Faculty Publications

Systemic acquired resistance (SAR) is induced following inoculation of Peronospora tabacina sporangia into the stems of Nicotiana tabacum plants highly susceptible to the pathogen. Previous results have shown that accumulation of acidic P-1,3-glucanases (PR-2's) following induction of SAR by P. tabacina may contribute to resistance to P. tabacina. We showed that up-regulation of the PR-2 gene, PR-2d, following stem inoculation with P. tabacina, is associated with SAR. Studies using plants transformed with GUS constructs containing the full length promoter from PR-2d or promoter deletions, provided evidence that a previously characterized regulatory element that is involved in response to …

Phylogenetic Relationships Of African Microhylid Frogs Inferred From Dna Sequences Of Mitochondrial 12s And 16s Rrna Genes, Simon P. Loader, David J. Gower, Kim M. Howell, Nike Doggart, Mark-Oliver Rödel, Barry T. Clarke, Rafael O. De Sá, Bernard L. Cohen, Mark Wilkinson

Biology Faculty Publications

The phylogenetic relationships of microhylid frogs are poorly understood. The first molecular phylogeny for continental African microhylids is presented, including representatives of all subfamilies, six of the eight genera, and the enigmatic hemisotid Hemisus. Mitochondrial 12S and 16S rRNA sequence data were analysed using parsimony, likelihood and Bayesian methods. Analyses of the data are consistent with the monophyly of all sampled subfamilies and genera. Hemisus does not nest within either brevicipitines or non-brevicipitines. It is possibly the sister group to brevicipitines, in which case brevicipitines might not be microhylids. Phrynomantis and Hoplophryne potentially group with non-African, non-brevicipitine microhylids, in …

Revision Of The Phyllophaga Of Hispaniola (Coleoptera: Scarabaeidae: Melolonthinae) — Part 2, Robert E. Woodruff, Milton W. Sanderson

Insecta Mundi

With this study, the fauna of Hispaniolan Phyllophaga is now composed of 48 species, all of which are endemic (precinctive), including 22 new species described herein (4 attributed to Woodruff and Sanderson: approxima, bonfils, jimenezi, rex; 18 to Woodruff: aceitillar, alcoa, androw, baoruco, carnegie, davidsoni, eladio, haitiensis, jaragua, larimar, marcano, nunezi, ortizi, pedernales, rawlinsi, rustica, santachloe, toni). Additionally, allotypes are described for 7 species with previously unknown males (aliada, canoa) or females (esquinada, fossoria, imprima, kenscoffi, panicula), and 6 new country records (Dominican Republic) are provided (aliada, leptospica, minutissima, panicula, permagna, recorta). Of …

Revision Of The Phyllophaga Of Hispaniola (Coleoptera: Scarabaeidae: Melolonthinae) — Part 3, Robert E. Woodruff, Milton W. Sanderson

Insecta Mundi

With this study, the fauna of Hispaniolan Phyllophaga is now composed of 48 species, all of which are endemic (precinctive), including 22 new species described herein (4 attributed to Woodruff and Sanderson: approxima, bonfils, jimenezi, rex; 18 to Woodruff: aceitillar, alcoa, androw, baoruco, carnegie, davidsoni, eladio, haitiensis, jaragua, larimar, marcano, nunezi, ortizi, pedernales, rawlinsi, rustica, santachloe, toni). Additionally, allotypes are described for 7 species with previously unknown males (aliada, canoa) or females (esquinada, fossoria, imprima, kenscoffi, panicula), and 6 new country records (Dominican Republic) are provided (aliada, leptospica, minutissima, panicula, permagna, recorta). Of …

Revision Of The Phyllophaga Of Hispaniola (Coleoptera: Scarabaeidae: Melolonthinae) — Part 5, Robert E. Woodruff, Milton W. Sanderson

Insecta Mundi

With this study, the fauna of Hispaniolan Phyllophaga is now composed of 48 species, all of which are endemic (precinctive), including 22 new species described herein (4 attributed to Woodruff and Sanderson: approxima, bonfils, jimenezi, rex; 18 to Woodruff: aceitillar, alcoa, androw, baoruco, carnegie, davidsoni, eladio, haitiensis, jaragua, larimar, marcano, nunezi, ortizi, pedernales, rawlinsi, rustica, santachloe, toni). Additionally, allotypes are described for 7 species with previously unknown males (aliada, canoa) or females (esquinada, fossoria, imprima, kenscoffi, panicula), and 6 new country records (Dominican Republic) are provided (aliada, leptospica, minutissima, panicula, permagna, recorta). Of …

Revision Of The Phyllophaga Of Hispaniola (Coleoptera: Scarabaeidae: Melolonthinae) — Part 1, Robert E. Woodruff, Milton W. Sanderson

Insecta Mundi

With this study, the fauna of Hispaniolan Phyllophaga is now composed of 48 species, all of which are endemic (precinctive), including 22 new species described herein (4 attributed to Woodruff and Sanderson: approxima, bonfils, jimenezi, rex; 18 to Woodruff: aceitillar, alcoa, androw, baoruco, carnegie, davidsoni, eladio, haitiensis, jaragua, larimar, marcano, nunezi, ortizi, pedernales, rawlinsi, rustica, santachloe, toni). Additionally, allotypes are described for 7 species with previously unknown males (aliada, canoa) or females (esquinada, fossoria, imprima, kenscoffi, panicula), and 6 new country records (Dominican Republic) are provided (aliada, leptospica, minutissima, panicula, permagna, recorta). Of …

Revision Of The Phyllophaga Of Hispaniola (Coleoptera: Scarabaeidae: Melolonthinae) — Part 4, Robert E. Woodruff, Milton W. Sanderson

Insecta Mundi

With this study, the fauna of Hispaniolan Phyllophaga is now composed of 48 species, all of which are endemic (precinctive), including 22 new species described herein (4 attributed to Woodruff and Sanderson: approxima, bonfils, jimenezi, rex; 18 to Woodruff: aceitillar, alcoa, androw, baoruco, carnegie, davidsoni, eladio, haitiensis, jaragua, larimar, marcano, nunezi, ortizi, pedernales, rawlinsi, rustica, santachloe, toni). Additionally, allotypes are described for 7 species with previously unknown males (aliada, canoa) or females (esquinada, fossoria, imprima, kenscoffi, panicula), and 6 new country records (Dominican Republic) are provided (aliada, leptospica, minutissima, panicula, permagna, recorta). Of …

Regulatory Role Of Glycogen Synthase Kinase 3 For Transcriptional Activity Of Add1/Srebp1c, Kang Ho Kim, Min Jeong Song, Eung Jae Yoo, Sung Sik Choe, Sang Dai Park, Jae Bum Kim

Faculty, Staff and Student Publications

Adipocyte determination- and differentiation-dependent factor 1 (ADD1) plays important roles in lipid metabolism and insulin-dependent gene expression. Because insulin stimulates carbohydrate and lipid synthesis, it would be important to decipher how the transcriptional activity of ADD1/SREBP1c is regulated in the insulin signaling pathway. In this study, we demonstrated that glycogen synthase kinase (GSK)-3 negatively regulates the transcriptional activity of ADD1/SREBP1c. GSK3 inhibitors enhanced a transcriptional activity of ADD1/SREBP1c and expression of ADD1/SREBP1c target genes including fatty acid synthase (FAS), acetyl-CoA carboxylase 1 (ACC1), and steroyl-CoA desaturase 1 (SCD1) in adipocytes and hepatocytes. In contrast, overexpression of GSK3beta down-regulated the transcriptional …

Multiple Testing Procedures: R Multtest Package And Applications To Genomics, Katherine S. Pollard, Sandrine Dudoit, Mark J. Van Der Laan

U.C. Berkeley Division of Biostatistics Working Paper Series

The Bioconductor R package multtest implements widely applicable resampling-based single-step and stepwise multiple testing procedures (MTP) for controlling a broad class of Type I error rates, in testing problems involving general data generating distributions (with arbitrary dependence structures among variables), null hypotheses, and test statistics. The current version of multtest provides MTPs for tests concerning means, differences in means, and regression parameters in linear and Cox proportional hazards models. Procedures are provided to control Type I error rates defined as tail probabilities for arbitrary functions of the numbers of false positives and rejected hypotheses. These error rates include tail probabilities …

Distance Between Pollen Donor And Recipient Influences Fruiting Success In Slickspot Peppergrass, Lepidium Papilliferum, Ian C. Robertson, Amy C. Ulappa

Biology Faculty Publications and Presentations

Plant populations are often spatially structured owing to limited dispersal of pollen and seed. Mating between neighboring individuals in such populations often leads to reduced reproductive performance relative to matings between distant individuals. This response, which may be a result of inbreeding depression or prezygotic mating barriers, was investigated for slickspot peppergrass, Lepidium papilliferum L. (Brassicaceae), a rare insect-pollinated mustard endemic to sagebrush–steppe habitat in southwestern Idaho. Through hand pollination experiments we found that individual plants receiving pollen from distant sources (75–100 m and 6.5–20 km away) had significantly higher percent fruit sets than those relying on pollen from neighboring …

Smokejumper Obituary: Wenger, Roy Emerson (Cps-103 Administrator 0000), National Smokejumper Association

Smokejumper Obituaries

No abstract provided.

Smokejumper Obituary: Hammer, Loren (Pilot 0000), National Smokejumper Association

Smokejumper Obituaries

No abstract provided.

Small Grains: 2005 Variety Recommendations (2004 Crop Performance Results), Cooperative Extension Service, South Dakota State University

SDSU Extension Circulars

Variety selection is a fundamental element in a sound crop production program. This report contains variety recommendations, descriptions, and yield data for the spring-seeded small grains – hard red spring wheat, oat, and barley, along with the fall-seeded small grain –hard red winter wheat. Key factors in variety selection include yield, yield stability, maturity, straw strength, height, test weight, quality, and disease resistance. Yield is an important factor; however, a variety with good disease resistance, straw strength, and high grain quality may be more profitable in some cases than the highest yielding variety. Disease resistance information is based on reactions …

Betty Bender, Betty Bender, Institute Of Child Nutrition. Child Nutrition Archives.

Oral History Project (all interviews)

Betty Bender, a native of Kentucky, was educated at the University of Kentucky, the University of Montana, and Duke University. After working in Massachusetts and Indiana, Bender married and moved to Dayton, OH, and worked for the Dayton School System for thirty years. While there she earned her master’s in Food Management. Betty was ASFSA President in 1983-84.

Sylvia Elam And Paul Mcelwain, Sylvia Elam, Paul Mcelwain, Institute Of Child Nutrition. Child Nutrition Archives

Oral History Project (all interviews)

Sylvia Elam’s involvement with the child nutrition profession goes back over thirty years. She is a native Kentuckian and holds an undergraduate degree in Home Economics from Morehead State University, with graduate work in Education. As a home economist she has worked in business and also taught at the secondary level. In the early 1970s she began work for the Kentucky Department of Education as a consultant in child nutrition. Since that time she has held various positions within the department from consultant all the way up to Assistant Director of Nutrition Services. She is now retired.

Paul McElwain has …

Atrazine Runoff In The Blue River Basin: Geomorphology, Rainfall, And Agronomic Practices, Kundan Dhakal

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

Atrazine concentrations in the Big Blue River Basin (BRB) in Nebraska and Kansas periodically exceed the U.S. EPA Maximum Contaminant Level (MCL) of 3 µg L^-1. The present study is focused on watershed variables influencing surface runoff of atrazine. The assessment includes the impact of corn and sorghum planting progress (indicating atrazine application), rainfall, antecedent soil water content, and soil restrictive layer on stream-measured weekly atrazine load in independent BRB subwatersheds for 1997 - 2004. Maximum atrazine loading occurred after most of the corn had been planted but during sorghum planting from mid-May to early June, immediately following …

Kinetics And Relative Importance Of Phosphorolytic And Hydrolytic Cleavage Of Cellodextrins And Cellobiose In Cell Extracts Of Clostridium Thermocellum, Yie.-Heng P. Zhang, Lee R. Lynd

Dartmouth Scholarship

Rates of phosphorolytic cleavage of 􏰁-glucan substrates were determined for cell extracts from Clostridium thermocellum ATCC 27405 and were compared to rates of hydrolytic cleavage. Reactions with cellopentaose and cellobiose were evaluated for both cellulose (Avicel)- and cellobiose-grown cultures, with more limited data also obtained for cellotetraose. To measure the reaction rate in the chain-shortening direction at elevated temper- atures, an assay protocol was developed featuring discrete sampling at 60°C followed by subsequent analysis of reaction products (glucose and glucose-1-phosphate) at 35°C. Calculated rates of phosphorolytic cleavage for cell extract from Avicel-grown cells exceeded rates of hydrolytic cleavage by >20-fold …

Effects Of Video Modeling On Skill Acquisition In Learning The Golf Swing, Joshua L. Smith

Theses and Dissertations

The purpose of this study was to determine the effects of video modeling on skill acquisition in learning the golf swing. One-hundred-eight college students participated in this study. All participants were pre-tested via videotaping to determine initial skill level. The pre-test videotaping was analyzed using DartTrainer software. Each participant was randomly assigned to one of three groups (1) control (2) single-view or (3) multi-view. Participants in the control group viewed a compact disc (CD) with a putting demonstration repeated 40 times. The participants in the single-view group viewed a CD with a front view only demonstration repeated 40 times of …

Identification Of Proteins Involved In Chloroplast Dna Replication, Matthew G. Lassen

Theses and Dissertations

Chapter 1

Chloroplast nucleoids (ct-nucleoids) are DNA/protein complexes involved in compacting the chloroplast genome, and may play a role in regulating DNA replication. Ct-nucleoids were isolated from young soybean plants and separated by 2-D gel electrophoresis. Gel spots were excised and analyzed by MALDI-ToF mass spectrometry, resulting in several protein identifications. The proteins identified all have functions unrelated to DNA replication. While some of these proteins may be due to contamination, it is possible that some of these proteins are dual-functional, playing direct roles in the regulation of DNA replication.

Chapter 2

A 28 kDa soybean protein was isolated by …

A Biomechanical Analysis Of Sprinters Vs. Distance Runners At Equal And Maximal Speeds, Tyler D. Bushnell

Theses and Dissertations

In the sport of track and field, sprinting and distance running represent two major categories of athletes. Sprinting is associated with power and speed, whereas distance running focuses on the economy of movement. With distance running there are elements of sprint technique that overlap. With distance events, there comes a time near the end of the race where economy gives way to speed. If the distance runners knew how to alter their technique in a way to become more sprint-like, this process could possibly be more successful. PURPOSE: This study compared the differences in technique between sprinters and distance runners …

Coral Reef Ecosystem Restoration Off Southeast Florida, David S. Gilliam, Walter Jaap, Richard E. Dodge, Elizabeth Glynn Fahy, Jamie A. Monty, Brian K. Walker, Lauren F. Shuman, B. D. Ettinger, Daniel P. Fahy, Shaun M. Gill, Richard Shaul

Marine & Environmental Sciences Faculty Proceedings, Presentations, Speeches, Lectures

Significant coral reef community development along the eastern shelf of the United States continues northward of the Florida Keys through Miami-Dade, Broward, Palm Beach, and Martin Counties, Florida (to Latitude 27° N). These Southeast Florida high-latitude coral communities have approximately 30 species of stony corals, stony coral coverage of 2-3%, and a diverse assemblage of reef gorgonians, sponges, and fishes.

This system lays within 3 km of the coast offshore a highly urbanized area comprising a population of over 5 million people (the population of Broward County alone exceeds 1.7 million). These reefs are important economic assets: a 2001 economic …

Center For Grassland Studies Newsletter, Winter 2004, Volume 10, No. 1

Center for Grassland Studies: Newsletters

Matching Beef Cattle Genetics to Feed Resources by Jim Gosey, Department of Animal Science, UNL

Ecological Monitoring to Assist Producers with Grassland Production Forecasts by George Green, School of Natural Resources, UNL

Nebraska Ranch Practicum Enters Sixth Year by Jerry Volesky, Don Adams, Richard Clark, Dillon Feuz, Patrick Reece, Brent Plugge, Bud Stolzenburg and Troy Walz, UNL

Bull Riders for Biodiversity? – And Other Heresies for the Northern Great Plains by Tyler Sutton, for The Conservation Alliance of the Great Plains

2004 Nebraska Grazing Conference to be Aug. 10-11

The Nebraska Turfgrass Conference: 42 Years of Educating Nebraska Turfgrass Managers …

2003 Coastal Municipal Stormwater Infrastructure Mapping Project, Jeffrey Marcoux

PREP Reports & Publications

This final report describes the grant program funded by NHEP and administered by DES. A Memorandum of Agreement (MOA) between NHEP and DES created a grant program to provide assistance to coastal communities to develop storm sewer infrastructure maps. DES issued a request for proposals (RFP), chose grant recipients, and managed the grant agreements. This report provides details on the grant projects completed by Farmington, Dover, Durham, Seabrook, Somersworth, and North Hampton. The deadline for completion of all grant projects was December 31, 2004.