Life Sciences Commons^™

Availability Of Use Of Total Extracellular Proteins In Sds-Page For Typing Staphylococcus Aureus And Coagulase-Negative Staphylococci, Sefa Can Saçilik, Özlem Osmanağaoğlu, A. Hakki Sayar, Cumhur Çökmüş

Turkish Journal of Biology

A total of nineteen Staphylococcus references, nine of which are Staphylococcus aureus and ten coagulase-negative Staphylococci (CNS), were characterized by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Whole-cell protein (WCP) profiles obtained by SDS-PAGE were compared with banding patterns produced by SDS-PAGE of total extracellular proteins (TEP). No report has yet been issued which explains Staphylococcus typing by SDS-PAGE of extracellular proteins. SDS-PAGE of whole-cell extracts did not usefully distinguish different isolates of Staphylococci. Our preliminary results suggest that SDS-PAGE of total extracellular proteins provides additional criteria and characteristic banding patterns for the study of the epidemiology and characterization of Staphylococci. …

Determination Of Some Fungal Metabolite As Influenced By Temperature, Time, Ph And Sugars By Bioassay Method, Meti̇n Diğrak, Sami̇ Özçeli̇k

Turkish Journal of Biology

This study investigated the fungal biomass and inhibition of Bacillus megaterium DSM 32 with extracts of Fusarium equiseti, Penicillium italicum, Penicillium digitatum, Aspergillus candidus and Aspergillus parasiticus in modified Czapek Dox Broth (mCDB) as affected by temperature (6, 15, 25 °C), incubation time (up to 4 wk), pH (3.0, 5.0 and 8.0) and glucose the and fructose (4.0, 6.0 and 9.0%). The biomass production was greatest at 25 °C. In general, biomass production was greatest in media containing an addition of 4.0% glucose or 6.0% fructose; however, bacterial inhibition production was reduced by elevated sugars in mCDB over a 4-wk …

Effects Of Cd^{2+}, Zn^{2+}, Li^+ And Al^{3+} On The Fertilization-Induced Membrane Potential Changes In The Egg Of The Frog Rana Cameranoi, Şeref Erdoğan, Gülay Loğoğlu, Kerem Tuncay Özgünen, Tuncay Özgünen

Turkish Journal of Biology

The apparent change in the egg membrane potential at fertilization gives rise to fertilization potential (FP). FP is the initial, transient electrical block to polyspermy as shown in most species especially those exhibiting external fertilization, and it protects the egg from a second sperm entry until the permanent, mechanical block is set up. Polyspermy risk is increased when this effective electrical block cannot reach a certain peak value or when it is of short duration. On the other hand, metal accumulation in the external environment is potentially toxic to living beings, and we aimed in the present study to evaluate …

Assessment Of Polymorphic Aflp Markers In Triticum Durum And Aegilops Sp., Aytül İnci̇rli̇, Hati̇ce Bi̇lgi̇ç, Mahi̇nur S. Akkaya

Turkish Journal of Biology

Compared to other Polymerase Chain Reaction (PCR) based molecular markers, the Amplified Fragment Length Polymorphism (AFLP) marker system probably requires more technical expertise for the sequential enzymatic reaction steps. In addition, the reproducibility of the AFLP marker patterns is much more dependent on the quality and the purity of the DNA samples to be processed. The present study describes the optimization of reaction conditions and determination of the most polymorphic selective amplification primer sets in a comparison of winter and spring type Turkish durum wheat varieties. AFLP products resulted in about 100 bands, and the number of polymorphic AFLP loci …

In Vitro Inhibition Of The Mycelial Growth Of Some Root Rot Fungi By Rhizobium Leguminosarum Biovar Phaseoli Isolates, İbrahi̇m Özkoç, Muhammet Hani̇fi̇ Deli̇veli̇

Turkish Journal of Biology

In solid medium, the effects of twenty-three Rhizobium leguminosarum biovar phaseoli isolates on the mycelium development of the phytopathogenic fungi of three different genera (Fusarium oxysporum, Pythium ultimum and Rhizoctonia solani) were tested in vitro. The experiments were achieved by applying the superimpose dual culture technique. Most of the rhizobia tested inhibited the fungal development. The inhibition rates displayed differences in accordance with the bacteria and fungi isolates utilized. However, the inhibitory effect of the rhizobia occurred most on the isolates of Fusarium and Pythium species (up to 30.35% and 29.75% respectively) and least on those of Rhizoctonia species (16.03%). …

Aspects Of The Taxonomy And Ecology Of The Ground Beetle (Coleoptera: Carabidae) Assemblage Of The Swan Coastal Plain : (With Particular Reference To Habitat Fragmentation And The Quindalup Dune System), Nadine A. Guthrie

Theses: Doctorates and Masters

Long term studies encompassing seasonal variation in abundance and species present, forming base-line phenological data, arc required to understand the responses of the carabid (Ground Beetle) fauna to major habitat changes. In addition, few habitats unaffected by human disturbance remain in Australia, and evaluation of these fauna in these areas is required before further habitat loss occurs. Generally an unknown group of terrestrial predatory invertebrates in Australia, carabids have been the subjects of few ecological studies in this country. This restricts the utility of the Carabidae in environmental or conservation assessment programs in Australia. A study was initiated to assess …

The Reproductive Biology And Temporal Distribution Of A Great Egret And Nankeen Night Heron Colony At The Perth Zoo, Robyn Phillimore

Theses: Doctorates and Masters

A colony of Great Egrets (Ardea alba) and Nankeen Night Herons (Nycticorax caledonicus) has existed at the Perth Zoo in Metropolitan Perth for over 25 years. The colony is particularly significant for the conservation and management of Great Egrets in Western Australia as it is the only colony located in the Metropolitan area. Baseline information of their breeding biology was needed to facilitate the development of management guidelines for the zoo colony. Foraging behaviour was used to highlight specific adaptations in hunting strategies and diet. However, it was not possible to observe foraging Nankeen Night Herons as they forage at …

Time Series Modelling Of The Environmental Factors Affecting The Daily Catch Rate Of Western Rock Lobster, Sukanlaya Srisurichan

Theses: Doctorates and Masters

The western rock lobster fishery is one of the most significant and valuable single species fisheries in Australia and in the world. It generates a gross commercial value of $200-300 million dollars per year for the economy of Western Australia. The impact of environmental factors on the daily catch rate of the western rock lobster is of particular interest to the W.A. Marine Research Laboratories, at the Ministry of Fisheries, Western Australia. Considerable time and effort has been invested into building and developing suitable models to measure such impact on this fishery. While past research has focussed on monthly or …

A New Species Of Marsupial Frog (Anura: Hylidae: Gastrotheca), Edgar Lehr, William Duellman, Cesar Aguilar

Scholarship

A new species of Gastrotheca from the southern end of the Cordillera Azul in Peru seems to be most closely related to G. lateollota known only from the Cordillera Huancabamba in northern Peru. Both of these species and G. marsupiata, monticola and peruana produce tadpoles, whereas the other species of Gastrotheea in Peruvian Andes have direct development.

The Effect Of Moderate +Gz On Cervical Muscle Strength Of Raaf Trainee Pilots Flying Pc-9 Aircraft, Elissa Jane Burton

Theses : Honours

External stimulus/loading initiates adaptations within skeletal muscle. Whilst performing flying manoeuvres under +Gz it has been previously found that the cervical area has the highest loading. The purpose of this study was to examine cervical muscle response to moderate +Gz force (+4-6Gz) loading generated during RAAF pilot training. Cervical muscle strength was monitored in nine RAAF pilots completing an eight-month flight training course and ten controls matched for gender, age, height and weight. Cervical muscle strength and range of movement were measured at baseline and at eight months using the Multi-Cervical Rehabilitation Unit (Hanoun, Canada). Also measured, using EMG, was …

The Effect Of The Moon Phase On The Daily Catch Rate Of King, Tiger And Endeavour Prawns In The Shark Bay And Exmouth Gulf Fisheries, Tenniel Harman

Theses : Honours

Two of the major commercial prawn fisheries in Western Australia arc located in Exmouth Gulf and Shark Bay. Prawn fishing is the third most valuable fishing industry in WA, producing an annual income of $62 million. These two fishing regions are considered fully exploited (Fisheries WA Home Page) and careful management is needed to produce and maintain them as sustainable fisheries. The Western Australian Marine Research Laboratories have developed detailed computer models which estimate the stock abundance of the prawn fisheries. This provides information on what sort of restrictions should be made to maintain a stainable fishery. The impact of …

Relationships Of Stemborer Damage And Plant Physical Conditions To Maize Yield In A Semi-Arid Zone Of Eastern Kenya, Josephine Moraa Songa, Zhou Guofa, William Allan Overholt

All Peer-Reviewed Publications

A field survey on maize stemborers, stemborer damage, maize plant physical conditions, and maize yield was conducted in eastern Kenya under natural conditions. Chilo partellus was the dominant stemborer accounting for 89.5 % of all borers. Stemborer damage greatly reduced maize yield, with tunnel lengths greater than 20 cm causing a 40 % reduction of potential yield. A 33 % yield loss was found in plants with more than one stemborer exit hole. Each stemborer (at harvest time) was correlated with a 8-10 % yield loss. Good plant physical characteristics significantly increased grain yield. Principle component analysis showed that stemborer …

Polymorphism For Wolbachia Infections In Eastern And Southern African Cotesia Sesamiae (Cameron) (Hymenoptera: Braconidae) Populations, Adele J. Ngi-Song, Moses B. Mochiah

All Peer-Reviewed Publications

Cotesia sesamiae (Cameron) (Hymenoptera: Braconidae) is an indigenous, gregarious, larval endo/parasitoid which is widely distributed in Africa and attacks mid- to late instars of stemborers. There is renewed interest in using Co. sesamiae for biological control to reduce the problems caused by stemborers. However, Wolbachia sp., a bacterial symbiont of some populations of Co. sesamiae, poses some threat to the successful use of the parasitoid for stemborer control. This bacterium's common effect is cytoplasmic incompatibility, between infected males and uninfected females. Polymerase chain reaction (PCR) tests were carried out on parasitoid samples collected from 5 locations in Kenya and 8 …

2001 Weed Science Research Report - Green Section, Ndsu Weed Science, Dept. Of Plant Sciences

Plant Sciences Weed Science Reports

The Weed Science team at North Dakota State University conduct field research in the State of North Dakota. This specific document reports on field research conducted in 2001. It includes work on small grains and the management of several weeds in North Dakota.

Annual Meeting Of The Iowa Academy Of Science

Journal of the Iowa Academy of Science: JIAS

No abstract provided.

Lessons From An Inventory Of The Ames, Iowa, Flora (1859-2000), William R. Norris, Deborah Q. Lewis, Mark P. Widrlechner, Jimmie D. Thompson, Richard O. Pope

Journal of the Iowa Academy of Science: JIAS

A botanical survey of the vascular flora of the "planning and zoning jurisdiction" of the city of Ames, Iowa (i.e., the area within a boundary 3.2 km beyond the current city limits) was compiled from 1990 to 2000. During this survey, 916 taxa (71 % native) were encountered within chis boundary. Literature reviews and a survey of Iowa State University's Ada Hayden Herbarium for specimens that had been collected in Ames since 1859 add 204 taxa to the flora. This total of 1,120 taxa exceeds the number of taxa known from any comparable area (including counties) in Iowa. We produced …

Back Cover

Journal of the Iowa Academy of Science: JIAS

No abstract provided.

Duane Isely (1918-2000): A Tribute, Stanley L. Welsh, Deborah Q. Lewis

Journal of the Iowa Academy of Science: JIAS

Duane Isely, Distinguished Professor Emeritus, Department of Botany, Iowa State University, Ames, Iowa, died on 6 December 2000. Dr. Isely was an outstanding plant taxonomist with expertise in other fields as well, especially in seed technology and weed identification and control. He was born in Bentonville, Arkansas, on 24 October 1918 into a family of academicians. His father, Dwight Isely, was professor and later Dean of Agriculture at the University of Arkansas, and his mother, Blessie Elise Dort Isely, also taught at the University of Arkansas and eventually received her Ph.D.

The Personal Is Political And The Political Can Be Very Bloody Personal : Family Farming In A Deregulated Dairy Industry, Terese Reid

Theses : Honours

This qualitative study considers the links between the political ideology that led to the deregulation of the dairy industry in July 2000 and the personal lived experiences of dairy farming families at a grass roots level. The post-deregulation experiences of dairy farming families within the South West region of Western are investigated through an engaged critical ethnographic methodology. A critical analysis of the political policies relating to dairy deregulation, is also incorporated within the study's methodological framework. The underlying aims of critical ethnography are emancipation, empowerment and liberation. The central purpose of this research is to give the daily farming …

The Annual Meeting Of The Iowa Academy Of Science April 20-21, 2001 [Program, 113th Meeting], Iowa Academy Of Science

Iowa Academy of Science Documents

Contents:

Program Summary --- 1
General Session Speakers --- 4
Awards --- 7
Symposia Programs --- 8
Special Events --- 8
Poster Sessions --- 9
Section Programs/Special Sessions --- 9
Agricultural Sciences --- 9
Anthropology --- 10
Botany --- 11
Cellular, Molecular & Microbiology --- 15
Chemical Education --- 13
Chemistry: Inorganic, Analytical & Physical --- 16
Chemistry: Organic & Biological --- 16
Conservation --- 17, 24
Earth Science Teaching --- 13
Elementary Science Teaching --- 13
Engineering --- 12
Geology --- 17, 19
Physics --- 20
Physiology --- 20
Psychology & Linguistics --- 10
Science Teaching --- 13, 22, …

2001 Weed Science Research Report - Yellow Section, Ndsu Weed Science, Dept. Of Plant Sciences

Plant Sciences Weed Science Reports

The Weed Science team at North Dakota State University conduct field research in the State of North Dakota. This specific document reports on field research conducted in 2001. It includes work on several crops including checkpea, wheat, potato, canola, flax and others.

Iowa Academy Of Science 113th Annual Meeting [2001]: Advance Program, Iowa Academy Of Science

Iowa Academy of Science Documents

No abstract provided.

Distribution And Species Composition Of Cereal Stemborers In The Eastern Zone Of Tanzania, E. Nsami, B. Pallangyo, V. Mcoo, C. O. Omwega

All Peer-Reviewed Publications

A survey was conducted in the four regions of Tanga, Morogoro, Coast and Dar es Salaam in eastern Tanzania to determine the distribution and species composition of cereal stemborers and their natural enemies. A total of 22 fields were sampled in Tanga, 28 in Morogoro, 17 in Coast and one in Dar es Salaam. Chilo parlellns was found to be the predominant species, accounting for 80 % of all stemborers collected. Bnsseolafnsca, Sesamia calamistis and Chilo orichalcociliellus made up 15,4 and less than 1 % of the stemborers collected respectively. Infestation levels varied with the age of the plants, whereby …

Farmers’ Perceptions Of Importance, Control Methods And Natural Enemies Of Maize Stemborers At The Kenya Coast, M. J. Bonhof, A. Van Huis, F. G. Kiros, N. Dlbogo

All Peer-Reviewed Publications

A survey among 240 resource-poor farmers at the Kenya coast indicated that insect pests were considered to be the main production constraint. Over 70% of respondents mentioned stemborers to be the most important insect pest. Unlike the eggs and adults, stemborer larvae and pupae were frequently seen by farmers. Alternative gramineous host plants for pests were common near maize fields and were often not destroyed until after the first rains. Nearly all farmers left dry stems and stubble in the field after harvest. Farmers knew of many methods for controlling stemborers, but only 32 and 56% of farmers in Kilifi …

Impact Of Solar Radiation Rainfall And Cannibalism On Disappearance Of Maize Stemborers In Kenya, M. J. Bonhof, W. A. Overholt

All Peer-Reviewed Publications

The effect of solar radiation, rainfall and cannibalism on the disappearance of eggs and larvae of the cereal stemborer Chilo partellus (Swinhoe) were studied. Disappearance and nonviability of egg batches was higher on plants exposed to solar radiation than on shaded plants. Significantly fewer small larvae were recovered from plants exposed to rainfall than from shielded plants. Cannibalism among larvae of the same size was not common except at high larval densities. Contrary to the common belief that disappearance is the result of prédation, these studies show that solar radiation and rainfall might also play an important role in the …

Properties Of A Factor In Eland Plasma That Inhibits Transformation Of Bloodstream-Form Trypanosoma Brucei Brucei, E. K. Nguu, E. O. Osir

All Peer-Reviewed Publications

Fresh plasma from the African eland, Taiirotragus oryx, contains a factor that inhibits the transformation of bloodstream-form Trypanosma bnicei bntcei into procyclic (midgut) forms. Heating of the plasma at 42, 50 and 60 °C for 30 min resulted in a 20, 38 and 40% loss of inhibitor activity respectively, whereas only negligible loss occurred below 42 C. Similarly, one and four freeze-thawing cycles resulted in 32 and 60% loss of activity respectively. Inactivation of the inhibitor activity, which occurred rapidly during storage (for example, 80% loss after 7 days at -20 °C) could not be stopped by the addition of …

Habitat Management Strategies For The Control Of Cereal Stemborers And Striga In Maize In Kenya, Z. R. Khan, J. A. Pickett, L. Wadhams, F. Muyekho

All Peer-Reviewed Publications

Maize is the principal food and cash crop for millions of people in the predominantly mixed crop-livestock farming systems in Kenya. Stemborers and striga (Striga hermonthica) are major constraints to increased maize production in eastern Africa. An intercropping and trap crop system has been developed, using a ‘push-pull’ strategy, for the control of stemborers in small scale maize farming systems. The ‘push-pull’ strategy involves trapping stemborers on highly susceptible trap plants (pull) and driving them away from the crop using repellent intercrops (push). Napier grass (Pennisetum piirpiirenm Schumach) and Sudan grass (Sorghum vulgare sudanense Stapf J are used as trap …

The Chemical Ecology Of Host Location By Parasitoids Of African Stemborers, Linnet S. Gohole, Adele J. Ngi-Song

All Peer-Reviewed Publications

During foraging, parasitoids of insect herbivores are aided by chemical compounds generally termed ‘infochemicals‘, to locate their hosts. The origin of the infochemicals varies; they may be derived from the herbivore itself or activities related to the biology of the herbivore, the host plant, or from interactions between the plant and the herbivore. This paper reviews the status of research in this area, and presents an account of the infochemicals used by African stemborer parasitoids during foraging, as well as recommending possible areas for further investigation. The information generated has potential for application in behaviour modification of parasitoids species in …

Pathogenicity And Antifeedant Activity Of Entomopathogenic Hyphomycetes To The Cowpea Leaf Beetle, Ootheca Mutabilis Shalberg, S. Ekesi

All Peer-Reviewed Publications

Conidial suspensions of four isolates each of Bcauvcria bassiana and Melnrhizhtm anisopliae were evaluated in the laboratory for their virulence to adult Ootlicca mntabilis. All the isolates tested were pathogenic to the beetle, but to varying degrees. Three of them, B. bassiana CPD 3 and CPD10 and M. anisopliae CPD 6 were superior to all other isolates in terms of mortality induced on adult beetle, LT50 and LC50, with B. bnssiana CPD 3 having the lowest LC50 value. In feeding assayi consumption by beetles treated with B. bnssiana CPD 3 was significantly reduced within 2 days treatment (DAT) and the …

Stemborer Research In Zimbabwe: Prospects For The Establishment Of Cotesia Flavipes Cameron, P. Clhnwada, C. O. Omvvega, W. A. Overholt

All Peer-Reviewed Publications

The stemborers Busseola fnsca (Fuller) and Chilo partellus (Svvinhoe) cause the highest economic damage to maize and sorghum in Zimbabwe. A number of studies on stemborer bioecology and management in Zimbabwe have been conducted, but most are still incomplete. The more important of these are studies on life history, incidence and distribution patterns, host plants, yield losses, chemical control, cultural control, host plant resistance, the use of sex pheromones, and biological control. These are discussed here in detail, and areas where further research is needed are pointed out. As part of an integrated strategy for managing stemborers in Zimbabwe, an …