Life Sciences Commons^™

Production Of Auxin And Abscisic Acid Byphanerochaete Chrysosporiumme446 Immobilizedon Polyurethane Foam, Serpi̇l Ünyayar, Eli̇f Ünal, Ali̇ Ünyayar

Turkish Journal of Biology

The fungus Phanerochaete chrysosporiumME446 was entrapped on polyurethane foam to synthesize auxin and abscisic acid (ABA). The maximum amounts of auxin produced on the 18 th day in both free and immobilized cells were 55.91mg/mL and 76.07mg/mL, respectively. Maximum ABA concentrations on the 12 th day in both free and immobilized cells were 6.87 mg/mL and 10.69 mg/mL, respectively. An immobilized system of auxin and ABA production resulted in a comparatively higher yield than free fungal mycelium.

Temporal And Spatial Determination Of Germinbiosynthesis In Wheat Tissues, Mahmut Çalişkan, Andrew C. Cuming

Turkish Journal of Biology

In the current study, the biosynthesis of germin was determined in the organs of wheat embryos during germination. The temporal and spatial determination of germin protein was carried out by employing an anti-germin sera in gel-blot analysis. Germin was detected in the coleoptile, roots and vascular transition region of and 6-day-old germinated wheat seedlings. It was shown that the leaf tissue acquires germin accumulation only at the late stage of germination. The pellet fraction of protein homogenate obtained from embryos at different ages revealed higher germin accumulation than the supernatant fraction from the same sources.

Cultural Characteristics Of Morchella Esculentamycelium On Some Nutrients, Peri̇han Güler, Oğuz Arkan

Turkish Journal of Biology

The cultural characteristics of Morchella esculenta mycelium were investigated in potato dextrose agar, malt extract agar and complete medium yeast plates prepared with casein (cas), Casamino Acids (caa), peptone (p) and sodium nitrate (NaNO 3 ). Aerial and vegetative mycelium types were determined in the agar media with cas, caa and p. The third colonial characteristic was observed in the agar media with NaNO 3 . In this media, a colonization increase in circular forms spreading from the centre to the edges was determined. Mycelium colonization periods were 20-24 days in agar media with NaNO 3 .

Bacteriocin Typing Of Some Turkish Isolates Ofpseudomonas Syringae Pv. Phaseolicola, Kiymet Güven

Turkish Journal of Biology

Eighty-six Pseudomonas syringae pv. phaseolicola isolates collected from different bean growing areas in Eskişehir were typed for the production of bacteriocin.All the isolates tested produced bacteriocin and 24 bacteriosin groups were determined. No correlation was found between the bacteriocin groups and geographical origin. Authentic isolates of the bacterium representing 3 different races were also tested for bacteriocin production and bacteriocin types did not correlate with the races.

Electrophoretic Analysis Of Candida Albicans Isolatescollected From Turkish Hospitals By Native-Polyacrylamidegel Electrophoresis (N-Page), Özlem Osmanağaoğlu, Sefa Can Saçilik, Ufuk Gündüz, Nurten Altinlar, Cumhur Çökmüş

Turkish Journal of Biology

Native-polyacrylamide gel electrophoresis (N-PAGE) was used to characterize Candida albicansstrains isolated from vaginal specimens of patients suffering from vaginitis in Ankara hospitals, Turkey. Three different protein band profiles were observed among these 38 C. albicansisolates when whole cell protein extracts of these strains were used in N-PAGE. 34 of the 38 isolates were shown to belong to a single group or clone with a dissemination percentage of 89, while three isolates belonged to another group with a percentage of 8. Furthermore only one C. albicans isolate was detected with a dissemination percentage of 3 and assigned to a third group. …

Protein Profiles And Prevalence Of Methicillinresistant Staphylococcus Aureus (Mrsa) In Gülhanemilitary Academy Hospital In Turkey, Sefa Can Saçilik, Özlem Osmanağaoğlu, Özgül Kisa, Ahmet Başustaoğlu, Cumhur Çökmüş

Turkish Journal of Biology

The present study was undertaken to determine the relationship between Methicillin-Resistant Staphylococcus aureus (MRSA) strains isolated from various clinical specimens in Gülhane Military Medical Academy, in Ankara, Turkey. In 1997, at this hospital, the percentage of S. aureus among all nosocomial isolates was 20% and the percentage of MRSA among these S. aureus isolates was 51%. Both sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) and Native-Page (N-PAGE) were used for typing MRSA isolates which have common antibiotic susceptibility test results. MRSA isolates were also evaluated according to the whole-cell proteins (WCPs) and filtrate proteins (FPs). Using these methods, we concluded that …

Availibility Of Use Of Total Extracellular Proteins Insds-Page For Characterization Of Gram-Positive Cocci, Sefa Can Saçilik, Özlem Osmanağaoğlu, Ufuk Gündüz, Cumhur Çökmüş

Turkish Journal of Biology

There are no reports in the literature dealing with gram-positive cocci typing by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) of extracellular proteins. In this study, Staphylococcus aureus, Staphylococcus warneri, Staphylococcus chromogenes, Micrococcus lysodeikticus and Micrococcus ssp. of the Micrococcaceae family, and Pediococcus halophilus, Streptococcus ssp. and Enterococcus faecalis of the Streptococcaceae family were characterized by SDS-PAGE of whole cell proteins (WCPs) and total extracellular proteins (TEPs). Comparison of SDS-PAGE of WCP profiles with banding patterns produced by that of TEPs provided additional criteria and characteristic banding patterns for the study of the characterization of gram-positive cocci. This method was found …

Nonspecific Esterase Patterns Of Rattus Norvegicus(Berkenhout, 1769) In Western Turkey, Reyhan Çolak, Nuri̇ Yi̇ği̇t, Ercüment Çolak, Mustafa Sözen, Şaki̇r Özkurt

Turkish Journal of Biology

Nonspecific esterases in the muscle, heart, kidney and liver tissues of wild rats (Rattus norvegicus) were examined by horizontal starch gel electrophoresis. Zymogram bands corresponding to Es-10 and Es-4 in the muscle, heart, kidney and liver homogenates, Es-3 and Es-17 in the kidney and liver, Es-18 in the liver tissue, Es-15 in kidney and liver, Es-2 in the muscle and heart, Es-16 in the heart tissue were detected. These findings showed that nonspecific esterases are very polymorphic and tissue-specific, and are not of taxonomic importance for the populations of Rattus norvegicus.

Antiviral Activity Of Some Plant Extracts On Thereplication Of Autographa Californicanuclearpolyhedrosis Virus, Ömer Ertürk, Zi̇hni̇ Demi̇rbağ, Ali̇ Osman Beldüz

Turkish Journal of Biology

The effect of crude extracts, obtained from Aconitum nasutum, Daphne glomerata, Hypericum androsaemum, Laurus nobilis, Nerium oleander, Olea europaea, Prunus laurocerasus, Punica granatum, Rhododendron caucasicum, and Urtica dioica on the replication of Autographa californica nuclear polyhedrosis virus (AcNPV) grown in Spodoptera frugiperda cell culture were investigated by observing changes in cytophatic effects, progeny virus concentration and viral protein (polyhedrin) synthesis. We determined an inhibitory effect on cytophatic changes, virus concentration and synthesis of polyhedrin by utilizing the extract of A conitum nasutum, Hypericum androsaemum, Laurus nobilis, Rhododendron caucasicum, and Urtica dioica plants. On the other hand, the effects of Daphne …

The Possibility Of Using Crustacean Waste Products(Cwp) On Rainbow Trout ( Oncorhynchus Mykiss)Feeding, Yeşi̇m Özoğul

Turkish Journal of Biology

Crustacean waste product (CWP) generated from the fishing industry could be used in diets for rainbow trout as an alternative animal protein. Some experiments have been undertaken with penaid species, showing that it is possible to partially replace fish meal with CWP in penaid feeds. This study has been designed to assess whether CWP could be used to partially replace fish meal in diets for rainbow trout. The results show that CWP improved the digestibility of the diet, yielding the highest performance, and also that the addition of large amounts of CWP to a low-protein diet did not depress appetite. …

Blood Serum Proteins Of The Genus Mesocricetusnehring, 1898 (Mammalia: Rodentia) In Turkey, Reyhan Çolak, Nuri̇ Yi̇ği̇t, Ercüment Çolak, Mustafa Sözen, Şaki̇r Özkurt

Turkish Journal of Biology

In this study, 18 live specimens collected from six localities in the distribution areas of Mesocricetus brandti and Mesocricetus auratus were examined using SDS-PAGE (sodium dodecyl sulphate-polyacrylamide gel electrophoresis). The findings showed that there is no difference in globulin and albumin proteins of M. brandti and M. auratus, and supported the claim that M. auratus is very closely related to M. brandti.

Humerus Primer Ossification Zone Development In Rat Fetuses: A Histological And Histochemical Study, İsmai̇l Üstünel, Gökhan Akkoyunlu, Erdoğan Kocamaz, Ramazan Demi̇r

Turkish Journal of Biology

In this investigation of the primary ossification zones of 14-18 day-old fetal rat humeri by histological, histochemical and histoenzymatical methods, 30 rat fetuses of the species Rattus norvegicus were used. The humeral diaphysis samples of the fetuses were subjected to alcian blue-heamatoxylin, periodic acid Schiff (PAS)-heamatoxylin, alcian blue-PAS-heamatoxylin, alizarin red and toluidine blue, alkaline phosphatase (ALP), adenosine triphosphatase (ATPase) and acid phophatase (ACP) staining techniques. The results can be summarized as follows: On day 14, no ossification signs were evident yet, whereas on day 15, the initiation of degenerations in some chondrocyte cytoplasms and the formation of osteoblastic cells on …

Production Of Uricase Enzyme From Aspergillus Niger And Determination Of Some Factors Affecting The Activity, Fi̇gen Ertan, Erol Aksöz

Turkish Journal of Biology

This study was undertaken to obtain uricase enzyme from Aspergillus niger and also to determine the production conditions and the effects of some factors on enzyme activity. The results of the experiments, in consideration of the production of the uricase enzyme and the factors affecting the production, demonstrated that enzyme activity was maximum when the production time was 3 days at a temperature of 30ºC with the initial pH at 6.0 and inductor concentration at 0.1%. It was determined that ammonium compounds, which were added to the production medium, inhibited enzyme synthesis, and that MgS0_4 has an important role in …

Isolation, Identification And Toxigenic Tests Of Aeromonas Hydrophila From Several Sources In Izmir, Ataç Uzel, Füsun Uçar

Turkish Journal of Biology

A total of 43 samples (4 sea water, 5 water, 4 river water, 10 oyster and 10 fish samples) from Izmir were examined for the presence of Aeromonas hydrophila. Alkaline peptone Water (APW) for the environmental samples and Trypticase Soy Broth with Ampicillin and Toluidine Blue (TSBA) for the food samples were used for enrichment of the samples. In isolation, 5 different selective media were used: DNase Agar with Toluidine blue and Ampicillin (DNTA), Aeromonas Agar (AA), Rimler Shotts Agar (RSA), mA Agar and Aeromonas Mediums Base (AMB). Identification of the isolates was performed according to Bergey's Manual of Determinative …

Determination Of Organophosphate Resistance-Dependent Over-Produce Esterase Allel Type With Electrophoresis In The Malaria Vector Species Anopheles Sacharovi And Culex Pipiens (Diptera: Culicidae), Ümi̇t Lüleyap, Davut Alpteki̇n, Hali̇l Kasap, Mülki̇ye Kasap

Turkish Journal of Biology

In this study, over-produce esterase allele types dependent on organophosphate resistance in mosquitoes were determined by cellulose acetate gel electrophoresis in the human malaria vector species Anopheles sacharovi and Culex pipiens in Çukurova (Adana). We found A_2 and A_5 allele bands with some unknown and unidentified gel bands in some An. sacharovi field samples, of which some bands seemed to match with over-produce esterase. We identified A_2-B_2 allele types for all the Cx. pipiens samples collected in this study.

Histological Changes Induced By Sialoadenectomy And Flutamide On The Epididymal Tissue And Epididymal Abnormalities, M.Aydin Ketani̇, Ahmet Ersay, Yusuf Nergi̇z, Mustafa Deni̇z, Şennur Ketani̇

Turkish Journal of Biology

In this study the purpose was to determine the effect of sialoadenectomy (Maternal Epidermal Growth Factor deficiency) on epididymal development and to compare it with that of antiandrogen (Flutamide) application.Forty 12-week-old, sexually mature and pregnant Sprague-Dawley rats were chosen as the animal model. They were divided into four groups; Group I : Control group, Group II : Sialoadenectomized (mated on 28^{th} day), Group III: Flutamide applied (between gestational day 11 and 21), Group IV: Sialoadenectomy + Flutamide applied. After birth, all male rats were observed morphologically on the 60th day and sacrificed on the 60^{th} day postpartum under general anaesthesia. …

Effects Of Endosulfan On The Isolated Sciatic Nerve Of The Frog, Ülkü Çömelekoğlu, Belgi̇n Büyükakilli, Aynur Avci Özge

Turkish Journal of Biology

The purpose of this study was to investigate the effect of endosulfan on the isolated sciatic nerve of the frog. The nerve was exposed to 0.5 ppm endosulfan for 60 min and then compound action potentials were recorded extracellulary. The latencies, amplitudes, durations of depolarization and repolarization and threshold voltage were measured (n=30). Conduction velocities were calculated using the nerve lengths and latency values of each nerve. It was observed that values of amplitude and duration of repolarization were significantly higher (p

Distribution Of The Bacteria Involved In The Nitrogen And Sulfur Cycle In İzmir Bay, Fatma Güngör, Füsun Uçar

Turkish Journal of Biology

Qualitative seasonal distributions of sulfate-reducing bacteria, colorless sulfur bacteria, nitrifying and denitrifying bacteria in the water and sediment samples in the inner part of İzmir Bay were investigated in relation to certain physico-chemical parameters (pH, oxygen, hydrogen sulfide, nitrate, nitrite, ammonium). Denitrifying bacteria and sulfate-reducing bacteria were present in the sediment and even in the aerobic water samples. Hydrogen sulfide was found almost in all water samples measured but it was at lower concentrations. It is suggested that denitrifying bacteria and sulfate reducing bacteria which produce hydrogen sulfide are involved in the mineralization of organic matter. Members of Thiobacillus and …

In Vitro Propagation Of Thymus Sipyleus Boiss. (Lamiaceae), Bengi̇ Baba Erdağ, A. Kami̇l Yürekli̇

Turkish Journal of Biology

In vitro propagation of Thymus sipyleus Boiss. (Lamiaceae), an endemic from western Anatolia was investigated. Sterilized seeds of the plant were germinated on modified Murashige-Skoog medium (mMS) and Heller's nutrient media. Then, seedlings were transferred to Murashige-Skoog basal medium containing 0.4 mg/l Naphtaleneacetic acid, (NAA) and 3 mg/l Benzyladenin (BA) and then they formed callus where they were in contact with the medium. Plantlets which regenerated on the same medium formed multiple shoots within 2 weeks and they were rooted after the 4th subculture.

The Feeding Of Brachionus Plicatilis (Rotifera) With Different Microalgae Species, Isochrysis Galbana Parke, Tetraselmis Chuii (Bucker), Rhinomonas Reticulata (Lucas) Novamizo, Pavlova Lutheri (Droop) Green And Chlorella Vulgaris Beijerinck, Seli̇n Sayin, Oya Işik, Sevi̇m Polat

Turkish Journal of Biology

In this study, the feeding of Brachionus plicatilis with different single cell microalgae species, Tetraselmis chuii, Isochrysis galbana, Pavlova lutheri, Chlorella vulgaris and Rhinomonas reticulata which are used widely for rotifer culture was investigated. At the first stage of the study, B. plicatilis was fed a mixture of T. chuii, C. vulgaris, I. galbana and R. reticulata the densities of which were 130x104, 650x10^4, 260x10^4 and 130x10^4 cell/ml respectively. The highest ingestion rate, namely 1643 cell/rot./min, was obtained with C. vulgaris. At the second stage, B. plicatilis was fed a the mixture of T. chuii and R. reticulata, of which …

Risk Of Imported Filariasis In Pakistan, M A. Beg

Department of Pathology and Laboratory Medicine

No abstract provided.

In Vitro Mutagenesis For Structure/Function Studies Of The Bacteriophage T4 Rega Protein And The Murine Ahr Protein, Estel Rick Barbour

MUSC Theses and Dissertations

The bacteriophage T4 regA protein translationally represses over 30 T4 early genes, including the regA gene, by binding to the translation initiation region of the T4 mRNAs (Karam et al., 1981). Although the recognition sequence has been at least partially characterized in a number regA sensitive mRNAs, there is no consensus sequence or structural feature that is recognized by regA in all transcripts (Winter et al., 1987; Webster et al., 1989; Unnithan et al., 1990). Recent studies have identified an RNA binding site on regA protein, but there remains a large gap in our understanding of how regA protein functions …

Variation In Boll Weevil (Coleoptera: Curculionidae) Captures In Pheromone Traps Arising From Wind Speed Moderation By Brush Lines, Thomas W. Sappington, Dale W. Spurgeon

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Paired trap lines of Þve boll weevil pheromone traps each were placed on opposite sides of a brush line at six different sites on a plantation in the Lower Rio Grande Valley of Texas. Temperature, wind speed, and wind direction were monitored with a nearby weather station. We observed a strong negative relationship between mean daily wind speed and total daily capture of boll weevils. About half of the day-to-day variation in weevil captures was explained by wind speed alone. In addition, our data indicate that much of the variation between traps within days may arise from differences in local …

Analysis Of Human Skin Emanations By Gas Chromatography/Mass Spectrometry. 2. Identification Of Volatile Compounds That Are Candidate Attractants For The Yellow Fever Mosquito (Aedes Aegypti), Ulrich R. Bernier, Daniel L. Kline, Donald Barnard, Carl Schreck, Richard A. Yost

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Volatile compounds emanated from human skin were studied by gas chromatography/mass spectrometry (GC/ MS). The purpose of this study was to identify compounds that may be human-produced kairomones which are used for host location by the mosquito, Aedes aegypti (L.). The procedure used to collect volatiles was chosen because of prior knowledge that attractive substances can be transferred from skin to glass by handling. Laboratory bioassays have shown that the residuum on the glass remains attractive to mosquitoes until the compounds of importance evaporate. The sampling and analytical procedures modeled the above-cited process as closely as possible except that the …

Hearing With The Mind's Eye, Gottfried Schlaug, Chi Chen, Dan Press, Andrea R. Halpern, Aisling Warde, Qun Chen, Alvaro Pascual-Leone

Faculty Journal Articles

No abstract provided.

Oxygen Metabolism In Plantlbacteria Interactions: Characterization Of The Oxygen Uptake Response Of Plant Suspension Cells, C. Jacyn Baker, E. W. Orlandi, Kenneth L. Deahl

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

In recent years the accumulation of reactive oxygen species (ROS) has been studied in plant cell suspension systems treated with bacterial pathogens. However, the associated utilization of molecular oxygen has not been well characterized. Using a multi-electrode oxygen analyser, the rates of oxygen consumption by tobacco cells during bacterial interactions were monitored. Heat-killed (HK) bacteria, which initiate an immediate ROS response in plant cells, were used as an elicitor to avoid complications of oxygen consumption by viable bacteria. An increase in oxygen uptake by the tobacco cells occurred within 4 min after addition of HK-bacteria and lasted for about 10 …

Inhibition Of Cysteine And Aspartyl Proteinases In The Alfalfa Weevil Midgut With Biochemical And Plant-Derived Proteinase Inhibitors, Stephen E. Wilhite, Thomas C. Elden, Joze Brzin, Ann C. Smigocki

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Proteolytic activities in alfalfa weevil (Hypera postica) larval midguts have been characterized. Effects of pH, thiol activators, low-molecular weight inhibitors, and proteinase inhibitors (PIs) on general substrate hydrolysis by midgut extracts were determined. Hemoglobinolytic activity was highest in the acidic to mildly acidic pH range, but was maximal at pH 3.5. Addition of thiolactivators dithiothreitol (DTT), 2-mercaptoethanol (2-ME), or l-cysteine had little effect on hemoglobin hydrolysis at pH 3.5, but enhanced azocaseinolytic activity two to three-fold at pH 5.0. The broad cysteine PI E-64 reduced azocaseinolytic activity by 64% or 42% at pH 5 in the presence or …

Functional Characterization Of Mitogen-Dependent And Self-Perpetuating Nb2 Lymphoma Cell Lines, Charlotte Cockrell

Theses and Dissertations

This investigation involved the functional characterization of four Nb2 sub lines (Nb2-U- 17, Nb2-l l , SFJCD l , and D5) for their mitogenic and apoptotic responses to DEX ± PRL treatment. Many protein factors have been implicated to be crucial in controlling the apoptotic and mitogenic pathways. In conjunction with functional studies, an immunocytochemical investigation of four protein signals (NFKB, IKBa, STAT 5b, and the GR) was undertaken in an attempt to establish a relationship between the functional responsiveness of a given cell line and the concentration of a particular protein marker. Comparison of the functional data obtained for …

Client-Centered Versus Traditional-Directive Educational Approach: A Randomized Study Of Exercises And Perceived Control Among Patients With Type 2 Diabetes, Melissa D. Olfert

Loma Linda University Electronic Theses, Dissertations & Projects

A patient’s adherence to self-management therapies in diabetes strongly influences health outcomes, health care utilization, and ultimately health care costs. Engaging in regular exercise therapy reduces risk factors associated with type 2 diabetes. The purpose of this randomized prospective study was to determine if a client-centered approach (CCA) toward diabetes education, verses a traditional-directive approach (TDA), would improve exercise participation, perceived control, intention to exercise, and glycemic control in patients with type 2 diabetes.

Fifty-nine type 2 patients were randomized into two groups: CCA and TDA. Diabetes education in the CCA group provided empowerment toward acquiring knowledge, skills, and responsibility …

Reproduction Partitioning In Polygyne Nests Of The Yellowjacket Vespula Squamosa, Stephanie A. Stewart

Masters Theses

No abstract provided.