Life Sciences Commons^™

Cytoplasmic Filament-Deficient Mutant Of Treponema Denticola Has Pleiotropic Defects, Jacques Izard, William A. Samsonoff, Ronald J. Limberger

Department of Food Science and Technology: Faculty Publications

In Treponema denticola, a ribbon-like structure of cytoplasmic filaments spans the cytoplasm at all stages of the cell division process. Insertional inactivation was used as a first step to determine the function of the cytoplasmic filaments. A suicide plasmid was constructed that contained part of cfpA and a nonpolar erythromycin resistance cassette (ermF and ermAM) inserted near the beginning of the gene. The plasmid was electroporated into T. denticola, and double-crossover recombinants which had the chromosomal copy of cfpA insertionally inactivated were selected. Immunoblotting and electron microscopy confirmed the lack of cytoplasmic filaments. The mutant was further …

Legume Logic Number 123

Legume Logic

Contents

Lupins on the menu in Europe

Lupin trials develop market opportunities

Farming Future

Pulse points

Viral diseases affect seed quality of pulses

From the lupin desk

Fast Transport Of Neurofilament Protein Along Microtubules In Squid Axoplasm, Veena Prahlad, Brian T. Helfand, George M. Langford, Ron D. Vale, Robert D. Goldman

Biology - All Scholarship

Using squid axoplasm as a model system, we have visualized the fast transport of non-filamentous neurofilament protein particles along axonal microtubules. This transport occurs at speeds of 0.5-1.0 microm/second and the majority of neurofilament particles stain with kinesin antibody. These observations demonstrate, for the first time, that fast (0.5-1.0 microm/second) transport of neurofilament proteins occurs along microtubules. In addition, our studies suggest that neurofilament protein can be transported as non-membrane bound, nonfilamentous subunits along axons, and that the transport is kinesin-dependent. Microtubule-based fast transport might therefore provide a mechanism for the distribution and turnover of neurofilament, and perhaps other cytoskeletal …

Hexapod Herald - Vol. 12, No. 5, November 2000

Hexapod Herald and Other Entomology Department Newsletters

Calendar of events

Grants

Welcome

Congratulations

Publications

Travel

From the office

The Use Of Live-Bait Traps For The Study Of Sylvatic Rhodnius Populations (Hemiptera: Reduviidae) In Palm Trees, F. Abad-Franch, F. Noireau, A. Paucar C., H. M. Aguilar V., C. Carpio C., J. Racines V.

University of Nebraska State Museum: Entomology Papers

Chagas disease is a major public health challenge for most Latin American countries. An initiative for the coordinated control of Chagas disease transmission throughout the Andean countries was launched in 1997. Since the early 1990s, control measures based on elimination of domestic/peridomestic triatomine colonies and screening of donor blood by serological testing have resulted in a reduction in incidence of ~70% in the Southern Cone countries (WHO, 1991; Dias & Schofield, 1999; Moncayo, 1999; WHO/CTD, 2000).

Kentucky Warbler (Vol. 76, No. 4), Kentucky Library Research Collections

The Kentucky Warbler

No abstract provided.

Forage News [2000-11], Department Of Plant And Soil Sciences, University Of Kentucky

Forage News

• Statewide Grazing Conference Set for Bowling Green
• Fall Grazing School
• Three-Cornered Alfalfa Hopper
• Funding Source for Livestock/Forage Development
• Forages at KCA
• Forage Testing
• Forage Testing Has Economic Value
• Upcoming Events

Monitoring Bacterial Transport By Stable Isotope Enrichment Of Cells, William Holben, Peggy H. Ostrom

Biological Sciences Faculty Publications

Understanding the transport and behavior of bacteria in the environment has broad implications in diverse areas, ranging from agriculture to groundwater quality, risk assessment, and bioremediation. The ability to reliably track and enumerate specific bacterial populations in the context of native communities and environments is key to developing this understanding. We report a novel bacterial tracking approach, based on altering the stable carbon isotope value (delta C-13) Of bacterial cells, which provides specific and sensitive detection and quantification of those cells in environmental samples. This approach was applied to the study of bacterial transport in saturated porous media. The transport …

Natural Antibiotic Resistance Of Bacteria Isolated From Larvae Of The Oil Fly, Helaeomyia Petrolei, Dana R. Kadavy, Jacob M. Hornby, Terry Haverkost, Kenneth W. Nickerson

Papers in Microbiology

Helaeomyia petrolei (oil fly) larvae inhabit the asphalt seeps of Rancho La Brea in Los Angeles, Calif. The culturable microbial gut contents of larvae collected from the viscous oil were recently examined, and the majority (9 of 14) of the strains were identified as Providencia spp. Subsequently, 12 of the bacterial strains isolated were tested for their resistance or sensitivity to 23 commonly used antibiotics. All nine strains classified as Providencia rettgeri exhibited dramatic resistance to tetracycline, vancomycin, bacitracin, erythromycin, novobiocin, polymyxin, colistin, and nitrofurantoin. Eight of nine Providencia strains showed resistance to spectinomycin, six of nine showed resistance to …

The International Lepidoptera Survey Newsletter, November 2000, International Lepidoptera Survey, Ron Gatrelle

International Lepidoptera Survey Newsletter

Contents

Chlosyne ismeria ismeria by Ron Gatrelle (page 1)

Announcement: TC-ISBN Taxonomically Correct - Index of Scientific Butterfly Names

The Taxonomic Report update

Donations urgently needed for the International Lepidoptera Survey

2001 subscription rate lowered For TTR volume 3

Nebline, November 2000

NEBLINE Newsletter Archive from Nebraska Extension in Lancaster County

Family Meals Nourish Body and Soul
Holiday Safety for Your Home
Houseplant Problems
Gifts for The Gardener
Winter Protection for Roses
Invisible Bites and Itches
America Recycles Day: Wednesday, November 15, 2000
Voracious Voles
Bugs in Your Firewood?
Grain Stubble Rental Rates
Assessing Alternative Ag Opportunities
Irrigation Well Data Available on Internet
Biosolids Cuts Production Costs and Improves Yields
Planting Designs With Wildlife in Mind
Sweet Dreams Little Tractor
Space Heater Cautions
Eat More Fruits for Good Health
Healthy Eating: Turkey Crescents
Serve Food Safely at Holiday or Party Buffets
Focus on Food
Family & Community Education (FCE): Clarice's Column …

Fish & Wildlife News: November/December 2000

Fish and Wildlife News

Contents:

Jones Named Deputy Director 2
New Guidelines Announced for Federal Aid 3
Landmark Legislation for Refuge System 4
Maine Salmon Given ESA Protection 7
Service a Presence in Fisheries Society 11
Information Portal Makes Life Easy 13
Region 5 Invests in People 15
New Refuge Dedicated in Montana 16
U.S. and Russia Sign Polar Bear Treaty 18
Fighting the War on Weeds 22
Mexican Students Flock to Alaska 28
Fish and Wildlife...In Brief 35

Wpa News 63 (2000), World Pheasant Association

Galliformes Specialist Group and Affiliated Societies: Newsletters

WPA News (November 2000), number 63

Published by the World Pheasant Association

Proposed Design Modifications To Reduce Risk Of Operating Rotary Field Mowers, Katie L. White, Larry G. Wells, Scott A. Shearer, Larry R. Piercy

Biosystems and Agricultural Engineering Faculty Publications

The primary objective of this project was to reduce risk of injury associated with operating a rotary mower driven by a tractor power take-off (PTO) by developing and evaluating design improvements and determining their economic feasibility. Researchers have concluded that alteration of machinery design has a greater impact on the reduction of accidents than safety training. Implementation of an Operator Presence Sensing System (OPSS) and removal of the PTO are the two injury-reducing, engineering modifications evaluated by this research. Hydraulic power allows this to occur by providing dynamic braking, few moving parts (removal of the PTO), and controllable power. A …

Producer Protection Act Of 2000, J. David Aiken

Cornhusker Economics

Agricultural production contracts are becoming more widespread in Nebraska. (Contract production should be distinguished from custom farming, which is not at issue here). Under ag production contracts, farmers may contract to produce crops or livestock typically for processors. Contracting is widespread in the broiler industry, and is becoming more common in the swine and beef industry, and for some crops. The Producer Protection Act of 2000 (PPA) is a proposed model state statute prepared by the National Association of Attorney Generals to deal with legal issues associated with agricultural contracting. The PPA was developed in order to avoid some problems …

Meeting Explores Sensor Technology For Remote, Interactive Aquatic Experiments, Kendra L. Daly

Marine Science Faculty Publications

Oceans, lakes, rivers, and groundwater are complex, dynamic environments in which physical, chemical, and biological processes occur over varying temporal and spatial scales (e.g., eddies, nutrient fluxes, patchiness of organisms, benthic processes, and pollution). In addition, deep, remote, or hostile systems such as hydrothermal vents and polar regions traditionally are poorly sampled, but are important to understanding global biogeochemical and hydrological cycles. In the coming decades, moored, cabled, and autonomous observatories will be used to investigate a spectrum of basic processes in aquatic environments. In anticipation of the need to develop or re‐engineer sensors to measure physical, chemical, biological, and …

Comparison Of Coastal Fringe And Interior Forests As Reserves For Marbled Murrelets On Vancouver Island, Alan E. Burger, Volker Bahn, Angeline R.M. Tillmanns

Biological Sciences Faculty Publications

Much of the protected habitat available to the threatened Marbled Murrelet Brachyramphus marmoratus and other old-growth associated species in the Pacific Northwest is in narrow strips along the coast (e.g., parks and scenic fringes). Using data over two years from three watersheds on southwest Vancouver Island, we show that such shoreline strip forests represent suboptimal habitat for murrelets. Murrelet detections, including circling and subcanopy behaviors, were significantly lower at 30 coastal stations (20–250 m from the shoreline edge) than at 30 interior stations (1.5–21.0 km inland). Densities of predators were significantly higher at the coastal stations. The coastal trees were …

Dna Binding Properties Of The Arabidopsis Floral Development Protein Aintegumenta, Staci Nole-Wilson, Beth A. Krizek

Faculty Publications

The Arabidopsis protein AINTEGUMENTA (ANT) is a member of a plant-specific family of transcription factors (AP2/EREBP) that share either one or two copies of an approximately 70 amino acid region called the AP2 repeat. DNA binding activity has been demonstrated previously for members of this family containing a single AP2 repeat. Using an in vitro selection procedure, the DNA binding specificity of the two AP2 repeat containing protein ANT was found to be 5'-gCAC(A/G)N(A/T)TcCC(a/g)ANG(c/t)-3'. This consensus site is much longer than sites recognized by proteins containing a single AP2 repeat and neither AP2 repeat of ANT was alone capable of …

Effect Of Harvest And Effective Population Size On Genetic Diversity In A Striped Bass Population, Marilyn Diaz, David S. Wethey, James Bulak, Bert Ely

Faculty Publications

A major factor that contributes to loss of genetic variation in natural populations is a small effective population size. In species with a complex life history that involves overlapping generations and delayed maturity, the impact of infrequent annual reproductive bottlenecks is likely to be small because effective population size is defined by the number of individuals contributing to a generation and not to a single year-class. The striped bass Morone saxatilis is a longlived species with overlapping generations and age structure, whose recreational and commercial importance has made it a target of intense harvest.We analyzed allele frequency fluctuation among juvenile …

Ichthyofauna And Biogeography Of Russell Fork (Big Sandy River - Ohio River), Steven L. Powers, Patrick A. Ceas

Southeastern Fishes Council Proceedings

No abstract provided.

Regional Southeastern Fishes Council Reports, Southeastern Fishes Council

Southeastern Fishes Council Proceedings

No abstract provided.

Front Matter, Southeastern Fishes Council

Southeastern Fishes Council Proceedings

No abstract provided.

Minutes, Business Meeting, Southeastern Fishes Council

Southeastern Fishes Council Proceedings

No abstract provided.

Number 41 (November 2000), Southern Fishes Council

Southeastern Fishes Council Proceedings

(November 2000) - Ichthyofauna and biogeography of Russell Fork (Big Sandy River - Ohio River). By Steven L. Powers and Patrick A. Ceas

Minutes, Business Meeting, 25th Annual Meeting, Southeastern Fishes Council Regional Southeastern Fishes Council Reports

Horse Creek Fen, Birdwood Creek And Kelly Ranch Inventory, Robert F. Steinauer

Nebraska Game and Parks Commission: White Papers, Conference Presentations, and Manuscripts

Horse Creek Fen preserve is a complex of wetland communities (Sandhills fen, Sandhills freshwater marsh, and northern sedge wet meadow) associated with Horse Creek, a ditched stream that drains the Allen Valley fen. Fen vegetation on the preserve is limited to approximately 20 acres on the western boundary of the preserve where it abuts the eastern end of the Allen Valley fen (Steinauer et al. 1996). The fen vegetation is surrounded primarily by Sandhills freshwater marsh, which in turn is surrounded by northern sedge wet meadow. Additional Sandhills freshwater marsh occurs near the eastern boundary of the preserve. The eastern …

Porcine Reproductive And Respiratory Syndrome Virus: Description Of Persistence In Individual Pigs Upon Experimental Infection, R. Allende, W. W. Laegreid, G. F. Kutish, J. A. Galeota, R. W. Wills, Fernando A. Osorio

Nebraska Center for Virology: Faculty Publications

We studied the persistence of porcine reproductive and respiratory syndrome virus (PRRSV) in individual experimentally infected pigs, during a period of up to 150 days postinfection (dpi). The results of this study suggest that the persistence of PRRSV involves continuous viral replication but that it is not a true steady-state persistent infection. The virus eventually clears the body and seems to do it in most of the animals by 150 dpi or shortly thereafter. High genetic stability was seen for several regions of the persistent PRRSV’s genome, although some consistent mutations in the genes of envelope glycoproteins and M protein …

Biomechanics And Energetics In Aquatic And Semiaquatic Mammals: Platypus To Whale, Frank E. Fish

Biology Faculty Publications

A variety of mammalian lineages have secondarily invaded the water. To locomote and thermoregulate in the aqueous medium, mammals developed a range of morphological, physiological, and behavioral adaptations. A distinct difference in the suite of adaptations, which affects energetics, is apparent between semiaquatic and fully aquatic mammals. Semiaquatic mammals swim by paddling, which is inefficient compared to the use of oscillating hydrofoils of aquatic mammals. Semiaquatic mammals swim at the water surface and experience a greater resistive force augmented by wave drag than submerged aquatic mammals. A dense, nonwettable fur insulates semiaquatic mammals, whereas aquatic mammals use a layer of …

Mortality Of Escherichia Coli O157:H7 In Two Soils With Different Physical And Chemical Properties, D. N. Mubiru, Mark S. Coyne, John H. Grove

Plant and Soil Sciences Faculty Publications

Wild and domesticated animals can harbor a pathogenic Escherichia coli strain designated as O157:H7. Potential health problems could occur if strain O157:H7 is a more robust survivor in defecated waste than commonly used indicator bacteria. A laboratory study was conducted to assess E. coli O157:H7 survival relative to a nonpathogenie E. coli strain in two soils with different physical and chemical characteristics. Bacteria in the inoculated soils were enumerated on a weekly basis for 8 wk using a most probable number (MPN) technique. First-order decay models were used to describe bacteria mortality in the soils. Decay series were described slightly …

From Hopeful Monsters To Homeotic Effects: Richard Goldschmidt's Integration Of Development, Evolution, And Genetics, Michael Dietrich

Dartmouth Scholarship

Richard Goldschmidts research on homeotic mutants from 1940 until his death in 1958 represents one of the first serious efforts to integrate genetics, development, and evolution. Using two different models, Goldschmidt tried to show how different views of genetic structure and gene action could provide a mechanism for rapid speciation. Developmental systems were emphasized in one model and a hierarchy of genetic structures in the other. While Goldschmidt tried to find a balance between development and genetics, critics, such as Sewall Wright, urged him and eventually helped him incorporate population dynamics into his models as well. As such, the history …

A Novel Penalty Scheme In Genetic Algorithms For Structural Design Optimization, Nanakorn Pruettha, Meesomklin Konlakartn

ASEAN Journal on Science and Technology for Development

A Novel Penalty Scheme in Genetic Algorithms For Structural Design Optimization